Salmonella phage Lumpael

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Murrayvirus; Salmonella virus Lumpael

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F346|A0A3G8F346_9CAUD Uncharacterized protein OS=Salmonella phage Lumpael OX=2488859 PE=4 SV=1
MM1 pKa = 7.07KK2 pKa = 8.37TTYY5 pKa = 11.04NLDD8 pKa = 3.22QMALFSAIAAAVGKK22 pKa = 8.52QAPGLPADD30 pKa = 3.41NRR32 pKa = 11.84ANVVIEE38 pKa = 4.02AANMIVAAYY47 pKa = 9.98ALTDD51 pKa = 3.49AEE53 pKa = 4.38YY54 pKa = 9.0MAKK57 pKa = 10.29KK58 pKa = 10.37AEE60 pKa = 4.07APIAVDD66 pKa = 3.91LQPTAEE72 pKa = 4.03QVEE75 pKa = 4.24AGIASLWDD83 pKa = 4.3TIPGAMDD90 pKa = 3.42QLCQDD95 pKa = 3.7VAEE98 pKa = 4.75EE99 pKa = 4.11DD100 pKa = 3.56QGDD103 pKa = 4.16LFSDD107 pKa = 3.79TVTFVWQAMIGKK119 pKa = 9.39RR120 pKa = 11.84PQQ122 pKa = 2.81

Molecular weight: 13.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8F3J9|A0A3G8F3J9_9CAUD Uncharacterized protein OS=Salmonella phage Lumpael OX=2488859 PE=4 SV=1
MM1 pKa = 8.01IPEE4 pKa = 4.32KK5 pKa = 10.23VQRR8 pKa = 11.84KK9 pKa = 9.53ADD11 pKa = 4.76DD12 pKa = 3.08ILARR16 pKa = 11.84YY17 pKa = 7.3YY18 pKa = 11.04ARR20 pKa = 11.84EE21 pKa = 4.06LSPKK25 pKa = 9.75RR26 pKa = 11.84LRR28 pKa = 11.84GFGKK32 pKa = 10.15VLAFNVGYY40 pKa = 9.58SWRR43 pKa = 11.84LVTWDD48 pKa = 4.45EE49 pKa = 4.51GEE51 pKa = 4.12TWDD54 pKa = 4.77LLSHH58 pKa = 6.52EE59 pKa = 4.83EE60 pKa = 4.27YY61 pKa = 10.93NNKK64 pKa = 8.11TRR66 pKa = 11.84GRR68 pKa = 11.84RR69 pKa = 11.84QRR71 pKa = 11.84LQVKK75 pKa = 9.25GKK77 pKa = 9.88RR78 pKa = 3.57

Molecular weight: 9.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12847

33

1008

221.5

24.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.139 ± 0.717

0.95 ± 0.175

6.375 ± 0.225

6.609 ± 0.424

3.106 ± 0.174

7.947 ± 0.405

1.767 ± 0.232

4.966 ± 0.306

5.418 ± 0.296

7.589 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.196

4.273 ± 0.293

4.772 ± 0.203

4.694 ± 0.467

6.001 ± 0.264

4.982 ± 0.255

5.83 ± 0.364

6.204 ± 0.274

1.58 ± 0.112

3.09 ± 0.168
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski