Amino acid dipepetide frequency for Exiguobacterium phage vB_EauM-23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.638AlaAla: 6.638 ± 1.085
0.262AlaCys: 0.262 ± 0.132
5.502AlaAsp: 5.502 ± 0.614
6.376AlaGlu: 6.376 ± 0.692
3.231AlaPhe: 3.231 ± 0.741
5.415AlaGly: 5.415 ± 1.013
1.223AlaHis: 1.223 ± 0.318
5.852AlaIle: 5.852 ± 0.856
5.153AlaLys: 5.153 ± 0.578
6.288AlaLeu: 6.288 ± 0.918
1.747AlaMet: 1.747 ± 0.569
4.017AlaAsn: 4.017 ± 0.687
1.397AlaPro: 1.397 ± 0.319
3.231AlaGln: 3.231 ± 0.537
3.581AlaArg: 3.581 ± 0.64
5.066AlaSer: 5.066 ± 0.743
5.939AlaThr: 5.939 ± 0.715
5.502AlaVal: 5.502 ± 0.919
1.135AlaTrp: 1.135 ± 0.292
2.882AlaTyr: 2.882 ± 0.598
0.0AlaXaa: 0.0 ± 0.0
Cys
0.175CysAla: 0.175 ± 0.182
0.087CysCys: 0.087 ± 0.078
0.524CysAsp: 0.524 ± 0.187
0.437CysGlu: 0.437 ± 0.191
0.349CysPhe: 0.349 ± 0.176
0.611CysGly: 0.611 ± 0.257
0.087CysHis: 0.087 ± 0.077
0.349CysIle: 0.349 ± 0.177
0.699CysLys: 0.699 ± 0.306
0.611CysLeu: 0.611 ± 0.217
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.175CysPro: 0.175 ± 0.124
0.349CysGln: 0.349 ± 0.165
0.349CysArg: 0.349 ± 0.16
0.437CysSer: 0.437 ± 0.259
0.349CysThr: 0.349 ± 0.203
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.087CysTyr: 0.087 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
4.454AspAla: 4.454 ± 0.698
0.262AspCys: 0.262 ± 0.148
3.406AspAsp: 3.406 ± 0.511
5.502AspGlu: 5.502 ± 0.74
2.62AspPhe: 2.62 ± 0.434
4.105AspGly: 4.105 ± 0.581
0.786AspHis: 0.786 ± 0.262
4.454AspIle: 4.454 ± 0.6
4.454AspLys: 4.454 ± 0.705
4.279AspLeu: 4.279 ± 0.603
1.921AspMet: 1.921 ± 0.38
3.231AspAsn: 3.231 ± 0.456
1.659AspPro: 1.659 ± 0.412
1.747AspGln: 1.747 ± 0.295
3.057AspArg: 3.057 ± 0.624
2.707AspSer: 2.707 ± 0.436
3.493AspThr: 3.493 ± 0.508
4.978AspVal: 4.978 ± 0.894
1.048AspTrp: 1.048 ± 0.275
2.358AspTyr: 2.358 ± 0.426
0.0AspXaa: 0.0 ± 0.0
Glu
6.987GluAla: 6.987 ± 0.824
0.524GluCys: 0.524 ± 0.213
4.716GluAsp: 4.716 ± 0.803
6.725GluGlu: 6.725 ± 0.976
2.62GluPhe: 2.62 ± 0.522
3.668GluGly: 3.668 ± 0.586
0.524GluHis: 0.524 ± 0.19
4.716GluIle: 4.716 ± 0.694
4.629GluLys: 4.629 ± 0.738
7.249GluLeu: 7.249 ± 0.842
3.144GluMet: 3.144 ± 0.604
2.009GluAsn: 2.009 ± 0.415
2.183GluPro: 2.183 ± 0.484
3.581GluGln: 3.581 ± 0.535
6.114GluArg: 6.114 ± 0.946
3.843GluSer: 3.843 ± 0.642
4.716GluThr: 4.716 ± 0.753
5.066GluVal: 5.066 ± 0.634
0.961GluTrp: 0.961 ± 0.336
3.93GluTyr: 3.93 ± 0.61
0.0GluXaa: 0.0 ± 0.0
Phe
3.493PheAla: 3.493 ± 0.574
0.524PheCys: 0.524 ± 0.214
3.843PheAsp: 3.843 ± 0.64
3.144PheGlu: 3.144 ± 0.567
1.834PhePhe: 1.834 ± 0.392
3.231PheGly: 3.231 ± 0.533
0.611PheHis: 0.611 ± 0.196
1.485PheIle: 1.485 ± 0.314
3.493PheLys: 3.493 ± 0.576
2.358PheLeu: 2.358 ± 0.447
1.485PheMet: 1.485 ± 0.42
1.747PheAsn: 1.747 ± 0.46
0.611PhePro: 0.611 ± 0.269
1.31PheGln: 1.31 ± 0.433
1.659PheArg: 1.659 ± 0.317
3.144PheSer: 3.144 ± 0.612
2.009PheThr: 2.009 ± 0.393
2.62PheVal: 2.62 ± 0.539
0.262PheTrp: 0.262 ± 0.144
1.135PheTyr: 1.135 ± 0.345
0.0PheXaa: 0.0 ± 0.0
Gly
5.852GlyAla: 5.852 ± 0.989
0.262GlyCys: 0.262 ± 0.147
4.192GlyAsp: 4.192 ± 0.579
5.24GlyGlu: 5.24 ± 0.828
2.358GlyPhe: 2.358 ± 0.431
5.153GlyGly: 5.153 ± 0.666
0.961GlyHis: 0.961 ± 0.315
3.406GlyIle: 3.406 ± 0.604
4.629GlyLys: 4.629 ± 0.567
7.336GlyLeu: 7.336 ± 0.765
1.921GlyMet: 1.921 ± 0.386
4.192GlyAsn: 4.192 ± 0.542
1.135GlyPro: 1.135 ± 0.243
2.096GlyGln: 2.096 ± 0.379
4.454GlyArg: 4.454 ± 0.582
5.328GlySer: 5.328 ± 0.956
3.843GlyThr: 3.843 ± 0.601
6.9GlyVal: 6.9 ± 0.889
0.873GlyTrp: 0.873 ± 0.28
3.493GlyTyr: 3.493 ± 0.707
0.0GlyXaa: 0.0 ± 0.0
His
1.135HisAla: 1.135 ± 0.266
0.262HisCys: 0.262 ± 0.137
0.786HisAsp: 0.786 ± 0.313
1.223HisGlu: 1.223 ± 0.422
0.611HisPhe: 0.611 ± 0.239
0.961HisGly: 0.961 ± 0.291
0.611HisHis: 0.611 ± 0.233
1.223HisIle: 1.223 ± 0.301
1.31HisLys: 1.31 ± 0.374
1.048HisLeu: 1.048 ± 0.246
0.349HisMet: 0.349 ± 0.222
0.786HisAsn: 0.786 ± 0.262
0.699HisPro: 0.699 ± 0.209
0.437HisGln: 0.437 ± 0.2
1.135HisArg: 1.135 ± 0.336
0.873HisSer: 0.873 ± 0.312
0.786HisThr: 0.786 ± 0.272
1.048HisVal: 1.048 ± 0.286
0.175HisTrp: 0.175 ± 0.125
1.135HisTyr: 1.135 ± 0.389
0.0HisXaa: 0.0 ± 0.0
Ile
5.24IleAla: 5.24 ± 0.802
0.087IleCys: 0.087 ± 0.082
5.066IleAsp: 5.066 ± 0.804
5.59IleGlu: 5.59 ± 0.734
1.223IlePhe: 1.223 ± 0.339
4.367IleGly: 4.367 ± 0.757
1.397IleHis: 1.397 ± 0.339
3.668IleIle: 3.668 ± 0.559
3.406IleLys: 3.406 ± 0.541
3.057IleLeu: 3.057 ± 0.541
1.659IleMet: 1.659 ± 0.276
3.581IleAsn: 3.581 ± 0.597
2.009IlePro: 2.009 ± 0.466
2.533IleGln: 2.533 ± 0.387
3.93IleArg: 3.93 ± 0.654
2.533IleSer: 2.533 ± 0.651
4.279IleThr: 4.279 ± 0.683
4.541IleVal: 4.541 ± 0.617
0.524IleTrp: 0.524 ± 0.222
1.921IleTyr: 1.921 ± 0.451
0.0IleXaa: 0.0 ± 0.0
Lys
5.328LysAla: 5.328 ± 0.651
0.437LysCys: 0.437 ± 0.253
3.057LysAsp: 3.057 ± 0.527
5.59LysGlu: 5.59 ± 0.928
3.057LysPhe: 3.057 ± 0.457
4.017LysGly: 4.017 ± 0.519
2.096LysHis: 2.096 ± 0.529
3.319LysIle: 3.319 ± 0.633
4.629LysLys: 4.629 ± 0.616
4.803LysLeu: 4.803 ± 0.672
2.445LysMet: 2.445 ± 0.519
2.969LysAsn: 2.969 ± 0.401
2.271LysPro: 2.271 ± 0.42
3.755LysGln: 3.755 ± 0.699
4.803LysArg: 4.803 ± 0.766
3.843LysSer: 3.843 ± 0.674
3.668LysThr: 3.668 ± 0.497
5.24LysVal: 5.24 ± 0.683
0.786LysTrp: 0.786 ± 0.291
2.271LysTyr: 2.271 ± 0.4
0.0LysXaa: 0.0 ± 0.0
Leu
7.074LeuAla: 7.074 ± 0.753
0.524LeuCys: 0.524 ± 0.2
4.803LeuAsp: 4.803 ± 0.767
5.415LeuGlu: 5.415 ± 0.666
3.668LeuPhe: 3.668 ± 0.609
6.9LeuGly: 6.9 ± 0.789
0.961LeuHis: 0.961 ± 0.363
4.716LeuIle: 4.716 ± 0.535
5.066LeuLys: 5.066 ± 0.766
6.114LeuLeu: 6.114 ± 0.946
2.707LeuMet: 2.707 ± 0.556
3.581LeuAsn: 3.581 ± 0.596
2.533LeuPro: 2.533 ± 0.426
2.096LeuGln: 2.096 ± 0.407
3.755LeuArg: 3.755 ± 0.601
4.978LeuSer: 4.978 ± 0.773
4.803LeuThr: 4.803 ± 0.786
4.454LeuVal: 4.454 ± 0.58
0.873LeuTrp: 0.873 ± 0.314
2.271LeuTyr: 2.271 ± 0.341
0.0LeuXaa: 0.0 ± 0.0
Met
2.969MetAla: 2.969 ± 0.616
0.175MetCys: 0.175 ± 0.126
1.048MetAsp: 1.048 ± 0.273
1.921MetGlu: 1.921 ± 0.385
1.048MetPhe: 1.048 ± 0.28
1.572MetGly: 1.572 ± 0.385
0.437MetHis: 0.437 ± 0.184
1.485MetIle: 1.485 ± 0.366
3.057MetLys: 3.057 ± 0.56
1.834MetLeu: 1.834 ± 0.369
0.961MetMet: 0.961 ± 0.323
1.223MetAsn: 1.223 ± 0.273
1.223MetPro: 1.223 ± 0.32
1.31MetGln: 1.31 ± 0.282
1.834MetArg: 1.834 ± 0.388
2.096MetSer: 2.096 ± 0.423
2.62MetThr: 2.62 ± 0.363
1.485MetVal: 1.485 ± 0.351
0.262MetTrp: 0.262 ± 0.125
0.262MetTyr: 0.262 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
3.231AsnAla: 3.231 ± 0.625
0.087AsnCys: 0.087 ± 0.095
2.62AsnAsp: 2.62 ± 0.54
3.057AsnGlu: 3.057 ± 0.59
1.485AsnPhe: 1.485 ± 0.296
5.415AsnGly: 5.415 ± 0.688
0.699AsnHis: 0.699 ± 0.24
3.231AsnIle: 3.231 ± 0.436
3.231AsnLys: 3.231 ± 0.425
2.62AsnLeu: 2.62 ± 0.391
0.524AsnMet: 0.524 ± 0.217
2.271AsnAsn: 2.271 ± 0.402
2.62AsnPro: 2.62 ± 0.61
1.135AsnGln: 1.135 ± 0.326
3.493AsnArg: 3.493 ± 0.568
3.057AsnSer: 3.057 ± 0.54
2.533AsnThr: 2.533 ± 0.562
2.271AsnVal: 2.271 ± 0.467
0.611AsnTrp: 0.611 ± 0.231
1.572AsnTyr: 1.572 ± 0.392
0.0AsnXaa: 0.0 ± 0.0
Pro
2.707ProAla: 2.707 ± 0.397
0.0ProCys: 0.0 ± 0.0
1.921ProAsp: 1.921 ± 0.448
2.358ProGlu: 2.358 ± 0.446
1.834ProPhe: 1.834 ± 0.357
2.445ProGly: 2.445 ± 0.518
0.786ProHis: 0.786 ± 0.226
1.223ProIle: 1.223 ± 0.374
2.358ProLys: 2.358 ± 0.561
2.533ProLeu: 2.533 ± 0.57
0.699ProMet: 0.699 ± 0.227
1.572ProAsn: 1.572 ± 0.355
1.135ProPro: 1.135 ± 0.292
1.31ProGln: 1.31 ± 0.354
0.961ProArg: 0.961 ± 0.271
2.009ProSer: 2.009 ± 0.439
1.572ProThr: 1.572 ± 0.434
2.271ProVal: 2.271 ± 0.345
0.175ProTrp: 0.175 ± 0.115
0.524ProTyr: 0.524 ± 0.194
0.0ProXaa: 0.0 ± 0.0
Gln
3.057GlnAla: 3.057 ± 0.413
0.175GlnCys: 0.175 ± 0.117
1.834GlnAsp: 1.834 ± 0.453
2.009GlnGlu: 2.009 ± 0.425
1.485GlnPhe: 1.485 ± 0.299
2.62GlnGly: 2.62 ± 0.463
0.437GlnHis: 0.437 ± 0.192
2.271GlnIle: 2.271 ± 0.442
2.707GlnLys: 2.707 ± 0.515
3.755GlnLeu: 3.755 ± 0.584
1.397GlnMet: 1.397 ± 0.296
2.009GlnAsn: 2.009 ± 0.448
0.786GlnPro: 0.786 ± 0.236
1.921GlnGln: 1.921 ± 0.378
1.921GlnArg: 1.921 ± 0.442
1.747GlnSer: 1.747 ± 0.382
2.445GlnThr: 2.445 ± 0.411
2.62GlnVal: 2.62 ± 0.438
0.349GlnTrp: 0.349 ± 0.156
0.786GlnTyr: 0.786 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
3.493ArgAla: 3.493 ± 0.71
0.437ArgCys: 0.437 ± 0.206
4.017ArgAsp: 4.017 ± 0.607
3.493ArgGlu: 3.493 ± 0.648
2.096ArgPhe: 2.096 ± 0.431
3.581ArgGly: 3.581 ± 0.631
1.485ArgHis: 1.485 ± 0.39
4.105ArgIle: 4.105 ± 0.721
3.319ArgLys: 3.319 ± 0.724
6.201ArgLeu: 6.201 ± 0.756
1.921ArgMet: 1.921 ± 0.395
2.445ArgAsn: 2.445 ± 0.512
1.572ArgPro: 1.572 ± 0.377
1.659ArgGln: 1.659 ± 0.38
4.192ArgArg: 4.192 ± 0.668
3.319ArgSer: 3.319 ± 0.456
3.057ArgThr: 3.057 ± 0.509
4.105ArgVal: 4.105 ± 0.634
0.786ArgTrp: 0.786 ± 0.337
1.921ArgTyr: 1.921 ± 0.499
0.0ArgXaa: 0.0 ± 0.0
Ser
3.493SerAla: 3.493 ± 0.641
0.175SerCys: 0.175 ± 0.119
3.057SerAsp: 3.057 ± 0.539
4.454SerGlu: 4.454 ± 0.673
2.882SerPhe: 2.882 ± 0.543
6.9SerGly: 6.9 ± 0.88
0.786SerHis: 0.786 ± 0.261
3.406SerIle: 3.406 ± 0.578
4.891SerLys: 4.891 ± 0.673
4.454SerLeu: 4.454 ± 0.814
1.31SerMet: 1.31 ± 0.306
2.795SerAsn: 2.795 ± 0.65
2.533SerPro: 2.533 ± 0.408
1.834SerGln: 1.834 ± 0.328
2.969SerArg: 2.969 ± 0.428
3.843SerSer: 3.843 ± 0.914
3.668SerThr: 3.668 ± 0.806
3.755SerVal: 3.755 ± 0.494
0.873SerTrp: 0.873 ± 0.361
1.834SerTyr: 1.834 ± 0.523
0.0SerXaa: 0.0 ± 0.0
Thr
5.852ThrAla: 5.852 ± 0.915
0.611ThrCys: 0.611 ± 0.251
3.755ThrAsp: 3.755 ± 0.58
4.192ThrGlu: 4.192 ± 0.632
2.882ThrPhe: 2.882 ± 0.602
5.328ThrGly: 5.328 ± 0.578
0.786ThrHis: 0.786 ± 0.279
4.541ThrIle: 4.541 ± 0.417
3.406ThrLys: 3.406 ± 0.5
3.93ThrLeu: 3.93 ± 0.58
1.31ThrMet: 1.31 ± 0.371
2.795ThrAsn: 2.795 ± 0.584
2.096ThrPro: 2.096 ± 0.418
1.921ThrGln: 1.921 ± 0.319
2.795ThrArg: 2.795 ± 0.382
3.319ThrSer: 3.319 ± 0.588
3.581ThrThr: 3.581 ± 0.494
5.066ThrVal: 5.066 ± 0.783
1.048ThrTrp: 1.048 ± 0.35
2.969ThrTyr: 2.969 ± 0.483
0.0ThrXaa: 0.0 ± 0.0
Val
5.59ValAla: 5.59 ± 0.721
0.524ValCys: 0.524 ± 0.236
3.93ValAsp: 3.93 ± 0.591
5.59ValGlu: 5.59 ± 0.62
2.969ValPhe: 2.969 ± 0.557
4.891ValGly: 4.891 ± 0.72
1.048ValHis: 1.048 ± 0.274
4.803ValIle: 4.803 ± 0.685
4.541ValLys: 4.541 ± 0.607
5.502ValLeu: 5.502 ± 1.004
1.485ValMet: 1.485 ± 0.29
2.533ValAsn: 2.533 ± 0.461
2.271ValPro: 2.271 ± 0.498
2.096ValGln: 2.096 ± 0.427
3.843ValArg: 3.843 ± 0.56
5.415ValSer: 5.415 ± 0.681
4.891ValThr: 4.891 ± 0.767
5.24ValVal: 5.24 ± 0.686
0.524ValTrp: 0.524 ± 0.207
2.358ValTyr: 2.358 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
0.786TrpAla: 0.786 ± 0.238
0.0TrpCys: 0.0 ± 0.0
0.699TrpAsp: 0.699 ± 0.249
0.873TrpGlu: 0.873 ± 0.239
0.524TrpPhe: 0.524 ± 0.179
0.786TrpGly: 0.786 ± 0.336
0.262TrpHis: 0.262 ± 0.175
0.524TrpIle: 0.524 ± 0.243
0.611TrpLys: 0.611 ± 0.241
0.786TrpLeu: 0.786 ± 0.228
0.873TrpMet: 0.873 ± 0.255
0.699TrpAsn: 0.699 ± 0.232
0.0TrpPro: 0.0 ± 0.0
0.524TrpGln: 0.524 ± 0.2
0.611TrpArg: 0.611 ± 0.199
0.611TrpSer: 0.611 ± 0.293
1.135TrpThr: 1.135 ± 0.388
1.223TrpVal: 1.223 ± 0.341
0.349TrpTrp: 0.349 ± 0.173
0.524TrpTyr: 0.524 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.882TyrAla: 2.882 ± 0.399
0.262TyrCys: 0.262 ± 0.152
1.747TyrAsp: 1.747 ± 0.373
4.716TyrGlu: 4.716 ± 0.723
1.223TyrPhe: 1.223 ± 0.3
1.659TyrGly: 1.659 ± 0.334
0.611TyrHis: 0.611 ± 0.238
1.834TyrIle: 1.834 ± 0.382
2.882TyrLys: 2.882 ± 0.518
2.533TyrLeu: 2.533 ± 0.469
0.873TyrMet: 0.873 ± 0.255
1.31TyrAsn: 1.31 ± 0.312
1.485TyrPro: 1.485 ± 0.392
1.485TyrGln: 1.485 ± 0.29
1.747TyrArg: 1.747 ± 0.449
1.747TyrSer: 1.747 ± 0.37
2.795TyrThr: 2.795 ± 0.529
1.659TyrVal: 1.659 ± 0.402
0.786TyrTrp: 0.786 ± 0.286
1.659TyrTyr: 1.659 ± 0.328
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (11451 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski