Streptococcus phage Javan638
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6BGW7|A0A4D6BGW7_9CAUD Major capsid protein OS=Streptococcus phage Javan638 OX=2548293 GN=Javan638_0030 PE=4 SV=1
MM1 pKa = 7.62 TLTFLDD7 pKa = 4.78 FFAGVGCFRR16 pKa = 11.84 RR17 pKa = 11.84 GLKK20 pKa = 10.2 LAGITCFGYY29 pKa = 10.85 YY30 pKa = 9.66 VDD32 pKa = 5.54 EE33 pKa = 4.58 IQTGIDD39 pKa = 2.95 KK40 pKa = 11.1 GQFTDD45 pKa = 3.83 MEE47 pKa = 4.43 VTYY50 pKa = 9.25 ITEE53 pKa = 4.23 DD54 pKa = 3.44 TNQLWEE60 pKa = 4.45 EE61 pKa = 4.15 VAPTLSKK68 pKa = 11.17
Molecular weight: 7.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.216
IPC2_protein 4.329
IPC_protein 4.151
Toseland 3.973
ProMoST 4.291
Dawson 4.126
Bjellqvist 4.279
Wikipedia 4.037
Rodwell 3.986
Grimsley 3.897
Solomon 4.101
Lehninger 4.062
Nozaki 4.253
DTASelect 4.406
Thurlkill 4.024
EMBOSS 4.05
Sillero 4.266
Patrickios 3.198
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.181
Protein with the highest isoelectric point:
>tr|A0A4D6BCG3|A0A4D6BCG3_9CAUD Portal protein OS=Streptococcus phage Javan638 OX=2548293 GN=Javan638_0028 PE=4 SV=1
MM1 pKa = 7.78 NDD3 pKa = 2.94 NQRR6 pKa = 11.84 RR7 pKa = 11.84 GIWKK11 pKa = 9.4 LRR13 pKa = 11.84 RR14 pKa = 11.84 DD15 pKa = 3.63 GFGYY19 pKa = 10.46 GAIAQMLNLSLGSVKK34 pKa = 10.25 QYY36 pKa = 10.58 CRR38 pKa = 11.84 RR39 pKa = 11.84 HH40 pKa = 5.73 LEE42 pKa = 4.13 LKK44 pKa = 10.93 GMGQFVKK51 pKa = 10.78 YY52 pKa = 10.3 QLDD55 pKa = 3.54 EE56 pKa = 4.48 GEE58 pKa = 5.04 RR59 pKa = 11.84 PYY61 pKa = 11.22 CKK63 pKa = 10.36 NCMKK67 pKa = 10.41 KK68 pKa = 9.81 LHH70 pKa = 6.69 HH71 pKa = 6.58 AVQGRR76 pKa = 11.84 PKK78 pKa = 9.96 KK79 pKa = 10.34 FCSNSCRR86 pKa = 11.84 AIWWRR91 pKa = 11.84 NHH93 pKa = 5.09 QSQHH97 pKa = 6.41 DD98 pKa = 4.05 KK99 pKa = 11.04 TKK101 pKa = 9.57 TAYY104 pKa = 10.17 DD105 pKa = 3.63 EE106 pKa = 4.54 LTCQNCGRR114 pKa = 11.84 SFLSYY119 pKa = 11.22 ANPTRR124 pKa = 11.84 KK125 pKa = 9.4 FCGHH129 pKa = 6.83 PCYY132 pKa = 10.56 VEE134 pKa = 3.41 YY135 pKa = 10.67 RR136 pKa = 11.84 FRR138 pKa = 11.84 KK139 pKa = 9.81 GVTNDD144 pKa = 2.98 NATQDD149 pKa = 3.13 GDD151 pKa = 3.74 YY152 pKa = 10.38 GTFSKK157 pKa = 11.14
Molecular weight: 18.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.918
IPC2_protein 8.975
IPC_protein 8.916
Toseland 9.472
ProMoST 9.399
Dawson 9.794
Bjellqvist 9.619
Wikipedia 9.97
Rodwell 10.028
Grimsley 9.853
Solomon 9.823
Lehninger 9.794
Nozaki 9.78
DTASelect 9.531
Thurlkill 9.648
EMBOSS 9.94
Sillero 9.794
Patrickios 4.762
IPC_peptide 9.823
IPC2_peptide 8.668
IPC2.peptide.svr19 7.934
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
46
0
46
10598
41
832
230.4
25.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.077 ± 0.353
0.878 ± 0.123
6.001 ± 0.408
7.077 ± 0.344
3.803 ± 0.188
6.964 ± 0.327
2.029 ± 0.143
5.85 ± 0.264
7.143 ± 0.199
9.181 ± 0.286
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.434 ± 0.148
4.595 ± 0.218
2.925 ± 0.209
4.303 ± 0.244
4.388 ± 0.292
6.643 ± 0.413
6.813 ± 0.367
6.539 ± 0.263
1.51 ± 0.117
3.85 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here