Cauliflower mosaic virus (strain CM-1841) (CaMV)
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P03546|MVP_CAMVC Movement protein OS=Cauliflower mosaic virus (strain CM-1841) OX=10644 GN=ORF I PE=1 SV=1
MM1 pKa = 7.73 AEE3 pKa = 4.5 SILDD7 pKa = 3.48 RR8 pKa = 11.84 TINRR12 pKa = 11.84 FWYY15 pKa = 10.5 NLGEE19 pKa = 4.56 DD20 pKa = 4.62 CLSEE24 pKa = 4.25 SQFDD28 pKa = 3.63 LMIRR32 pKa = 11.84 LMEE35 pKa = 4.36 EE36 pKa = 4.14 SLDD39 pKa = 3.54 GDD41 pKa = 3.95 QIIDD45 pKa = 3.92 LTSLPSDD52 pKa = 3.49 NLQVEE57 pKa = 4.7 QVMTTTDD64 pKa = 3.35 DD65 pKa = 4.2 SISEE69 pKa = 4.02 EE70 pKa = 4.57 SEE72 pKa = 3.64 FLLAIGEE79 pKa = 4.16 ISEE82 pKa = 4.84 DD83 pKa = 3.61 EE84 pKa = 4.21 SDD86 pKa = 3.66 SGEE89 pKa = 4.04 EE90 pKa = 4.03 PEE92 pKa = 4.39 FEE94 pKa = 4.26 QVRR97 pKa = 11.84 MDD99 pKa = 3.4 RR100 pKa = 11.84 TGGTEE105 pKa = 3.59 IPKK108 pKa = 10.47 EE109 pKa = 3.95 EE110 pKa = 4.82 DD111 pKa = 3.31 GEE113 pKa = 4.73 GPSRR117 pKa = 11.84 YY118 pKa = 9.29 NEE120 pKa = 4.24 RR121 pKa = 11.84 KK122 pKa = 9.81 RR123 pKa = 11.84 KK124 pKa = 7.47 TPEE127 pKa = 3.29 DD128 pKa = 4.14 RR129 pKa = 11.84 YY130 pKa = 10.74 FPTQPKK136 pKa = 8.96 TIPGQKK142 pKa = 6.7 QTSMGMLNIDD152 pKa = 3.45 CQINRR157 pKa = 11.84 RR158 pKa = 11.84 TLIDD162 pKa = 3.36 DD163 pKa = 4.03 WAAEE167 pKa = 3.64 IGLIVKK173 pKa = 7.4 TNRR176 pKa = 11.84 EE177 pKa = 4.21 DD178 pKa = 3.71 YY179 pKa = 10.97 LDD181 pKa = 4.59 PEE183 pKa = 4.86 TILLLMEE190 pKa = 5.35 HH191 pKa = 6.19 KK192 pKa = 9.65 TSGIAKK198 pKa = 8.99 EE199 pKa = 4.39 LIRR202 pKa = 11.84 NTRR205 pKa = 11.84 WNRR208 pKa = 11.84 TTGDD212 pKa = 3.23 IIEE215 pKa = 4.15 QVINAMYY222 pKa = 10.9 TMFLGLNYY230 pKa = 10.01 SDD232 pKa = 4.16 NKK234 pKa = 9.76 VAEE237 pKa = 4.95 KK238 pKa = 9.96 IDD240 pKa = 3.71 EE241 pKa = 4.11 QEE243 pKa = 3.87 KK244 pKa = 10.81 AKK246 pKa = 10.57 IRR248 pKa = 11.84 MTKK251 pKa = 10.09 LQLFDD256 pKa = 2.96 ICYY259 pKa = 10.29 LEE261 pKa = 5.62 EE262 pKa = 4.13 FTCDD266 pKa = 3.39 YY267 pKa = 9.76 EE268 pKa = 5.13 KK269 pKa = 11.56 NMYY272 pKa = 7.76 KK273 pKa = 9.52 TEE275 pKa = 3.96 MADD278 pKa = 3.35 FPGYY282 pKa = 10.03 INQYY286 pKa = 9.44 LSKK289 pKa = 10.49 IPIIGEE295 pKa = 3.82 KK296 pKa = 10.07 ALTRR300 pKa = 11.84 FRR302 pKa = 11.84 HH303 pKa = 5.1 EE304 pKa = 4.41 ANGTSIYY311 pKa = 10.98 SLGFAAKK318 pKa = 9.37 IVKK321 pKa = 10.16 EE322 pKa = 4.25 EE323 pKa = 3.98 LSKK326 pKa = 10.93 ICDD329 pKa = 3.44 LSKK332 pKa = 10.64 KK333 pKa = 8.32 QKK335 pKa = 10.13 KK336 pKa = 9.23 LKK338 pKa = 10.43 KK339 pKa = 9.92 FNKK342 pKa = 8.29 KK343 pKa = 9.46 CCSIGEE349 pKa = 4.05 ASVEE353 pKa = 3.97 YY354 pKa = 10.54 GGKK357 pKa = 8.25 KK358 pKa = 7.83 TSKK361 pKa = 9.95 KK362 pKa = 9.95 KK363 pKa = 7.8 YY364 pKa = 7.47 HH365 pKa = 5.97 KK366 pKa = 9.95 RR367 pKa = 11.84 YY368 pKa = 7.74 KK369 pKa = 10.15 KK370 pKa = 9.84 RR371 pKa = 11.84 YY372 pKa = 8.78 KK373 pKa = 10.27 VYY375 pKa = 10.7 KK376 pKa = 9.62 PYY378 pKa = 10.61 KK379 pKa = 8.37 KK380 pKa = 9.95 KK381 pKa = 10.76 KK382 pKa = 8.5 KK383 pKa = 9.57 FRR385 pKa = 11.84 SGKK388 pKa = 8.01 YY389 pKa = 8.61 FKK391 pKa = 10.48 PKK393 pKa = 9.37 EE394 pKa = 4.11 KK395 pKa = 10.27 KK396 pKa = 8.74 GSKK399 pKa = 9.49 RR400 pKa = 11.84 KK401 pKa = 9.66 YY402 pKa = 9.14 CPKK405 pKa = 10.31 GKK407 pKa = 9.77 KK408 pKa = 9.42 DD409 pKa = 3.64 CRR411 pKa = 11.84 CWICNIEE418 pKa = 3.72 GHH420 pKa = 5.91 YY421 pKa = 11.26 ANEE424 pKa = 4.29 CPNRR428 pKa = 11.84 QSSEE432 pKa = 3.98 KK433 pKa = 10.95 AHH435 pKa = 6.69 ILQQAEE441 pKa = 4.19 NLGLQPVEE449 pKa = 4.07 EE450 pKa = 4.93 PYY452 pKa = 11.05 EE453 pKa = 4.26 GVQEE457 pKa = 4.31 VFILEE462 pKa = 4.23 YY463 pKa = 10.61 KK464 pKa = 10.49 EE465 pKa = 4.56 EE466 pKa = 4.1 EE467 pKa = 4.44 EE468 pKa = 4.21 EE469 pKa = 4.39 TSTEE473 pKa = 3.97 EE474 pKa = 4.75 SDD476 pKa = 5.02 DD477 pKa = 3.84 EE478 pKa = 4.97 SSTSEE483 pKa = 5.36 DD484 pKa = 3.35 SDD486 pKa = 3.84 SDD488 pKa = 3.58
Molecular weight: 56.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.183
IPC2_protein 5.283
IPC_protein 5.219
Toseland 5.13
ProMoST 5.283
Dawson 5.181
Bjellqvist 5.321
Wikipedia 5.016
Rodwell 5.105
Grimsley 5.054
Solomon 5.181
Lehninger 5.13
Nozaki 5.283
DTASelect 5.397
Thurlkill 5.13
EMBOSS 5.054
Sillero 5.372
Patrickios 4.177
IPC_peptide 5.181
IPC2_peptide 5.372
IPC2.peptide.svr19 5.355
Protein with the highest isoelectric point:
>sp|P03552|VAP_CAMVC Virion-associated protein OS=Cauliflower mosaic virus (strain CM-1841) OX=10644 GN=ORF III PE=3 SV=1
MM1 pKa = 7.71 SITGQPHH8 pKa = 6.08 VYY10 pKa = 10.34 KK11 pKa = 10.15 KK12 pKa = 10.26 DD13 pKa = 3.28 TIIRR17 pKa = 11.84 LKK19 pKa = 10.51 PLSLNSNNRR28 pKa = 11.84 SYY30 pKa = 11.62 VFSSSKK36 pKa = 11.2 GNIQNIINHH45 pKa = 6.59 LNNLNEE51 pKa = 3.99 IVGRR55 pKa = 11.84 SLLGIWKK62 pKa = 9.14 INSYY66 pKa = 10.68 FGLSKK71 pKa = 10.89 DD72 pKa = 3.5 PSEE75 pKa = 4.67 SKK77 pKa = 10.77 SKK79 pKa = 10.86 NPSVFNTAKK88 pKa = 10.78 NIFKK92 pKa = 10.78 SRR94 pKa = 11.84 GVDD97 pKa = 3.2 YY98 pKa = 10.92 SSQLKK103 pKa = 8.92 EE104 pKa = 4.12 VKK106 pKa = 10.33 SLLEE110 pKa = 4.07 AQNTRR115 pKa = 11.84 IKK117 pKa = 10.7 NLEE120 pKa = 3.9 NAIQSLDD127 pKa = 3.51 NKK129 pKa = 10.23 IEE131 pKa = 4.11 PEE133 pKa = 4.1 PLTKK137 pKa = 10.77 EE138 pKa = 3.86 EE139 pKa = 4.05 VKK141 pKa = 10.52 EE142 pKa = 3.89 LKK144 pKa = 10.55 EE145 pKa = 4.32 SINSIKK151 pKa = 10.61 EE152 pKa = 3.7 GLKK155 pKa = 10.77 NIIGG159 pKa = 3.69
Molecular weight: 17.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.844
IPC2_protein 8.99
IPC_protein 8.873
Toseland 9.926
ProMoST 9.443
Dawson 10.058
Bjellqvist 9.633
Wikipedia 10.16
Rodwell 10.76
Grimsley 10.101
Solomon 10.087
Lehninger 10.072
Nozaki 9.867
DTASelect 9.648
Thurlkill 9.926
EMBOSS 10.292
Sillero 9.955
Patrickios 10.482
IPC_peptide 10.087
IPC2_peptide 7.966
IPC2.peptide.svr19 7.945
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2302
129
679
383.7
43.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.039 ± 0.733
1.564 ± 0.273
5.3 ± 0.511
8.036 ± 1.12
3.779 ± 0.583
5.083 ± 0.373
2.042 ± 0.488
8.08 ± 0.428
10.556 ± 0.457
8.558 ± 0.598
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.215 ± 0.244
5.995 ± 1.141
4.605 ± 0.662
4.605 ± 0.316
3.736 ± 0.454
7.124 ± 0.838
5.343 ± 0.559
4.387 ± 0.579
0.869 ± 0.168
3.084 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here