Amino acid dipepetide frequency for Escherichia phage e4/1c

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.135AlaAla: 8.135 ± 0.827
0.487AlaCys: 0.487 ± 0.158
4.38AlaAsp: 4.38 ± 0.654
4.589AlaGlu: 4.589 ± 0.83
2.573AlaPhe: 2.573 ± 0.463
6.675AlaGly: 6.675 ± 0.602
1.46AlaHis: 1.46 ± 0.43
6.605AlaIle: 6.605 ± 0.798
6.119AlaLys: 6.119 ± 1.21
7.092AlaLeu: 7.092 ± 0.724
2.086AlaMet: 2.086 ± 0.38
3.963AlaAsn: 3.963 ± 0.502
1.738AlaPro: 1.738 ± 0.255
3.407AlaGln: 3.407 ± 0.424
4.311AlaArg: 4.311 ± 0.582
6.188AlaSer: 6.188 ± 0.678
3.963AlaThr: 3.963 ± 0.43
5.145AlaVal: 5.145 ± 0.648
1.252AlaTrp: 1.252 ± 0.299
2.295AlaTyr: 2.295 ± 0.36
0.0AlaXaa: 0.0 ± 0.0
Cys
0.487CysAla: 0.487 ± 0.193
0.07CysCys: 0.07 ± 0.075
0.626CysAsp: 0.626 ± 0.159
0.973CysGlu: 0.973 ± 0.257
0.834CysPhe: 0.834 ± 0.271
1.113CysGly: 1.113 ± 0.364
0.209CysHis: 0.209 ± 0.113
0.348CysIle: 0.348 ± 0.153
0.973CysLys: 0.973 ± 0.266
0.765CysLeu: 0.765 ± 0.216
0.348CysMet: 0.348 ± 0.153
0.765CysAsn: 0.765 ± 0.257
0.278CysPro: 0.278 ± 0.184
0.348CysGln: 0.348 ± 0.152
0.904CysArg: 0.904 ± 0.312
0.973CysSer: 0.973 ± 0.307
0.695CysThr: 0.695 ± 0.228
1.182CysVal: 1.182 ± 0.306
0.278CysTrp: 0.278 ± 0.144
0.348CysTyr: 0.348 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
4.728AspAla: 4.728 ± 0.755
0.278AspCys: 0.278 ± 0.142
4.102AspAsp: 4.102 ± 0.518
3.963AspGlu: 3.963 ± 0.545
2.503AspPhe: 2.503 ± 0.35
7.509AspGly: 7.509 ± 0.834
0.765AspHis: 0.765 ± 0.249
3.685AspIle: 3.685 ± 0.482
4.798AspLys: 4.798 ± 0.457
3.546AspLeu: 3.546 ± 0.512
1.043AspMet: 1.043 ± 0.253
2.99AspAsn: 2.99 ± 0.422
1.669AspPro: 1.669 ± 0.256
1.669AspGln: 1.669 ± 0.355
2.434AspArg: 2.434 ± 0.478
3.894AspSer: 3.894 ± 0.587
3.198AspThr: 3.198 ± 0.493
4.033AspVal: 4.033 ± 0.559
0.417AspTrp: 0.417 ± 0.169
3.059AspTyr: 3.059 ± 0.46
0.0AspXaa: 0.0 ± 0.0
Glu
5.006GluAla: 5.006 ± 0.708
0.973GluCys: 0.973 ± 0.314
2.712GluAsp: 2.712 ± 0.44
4.033GluGlu: 4.033 ± 0.643
3.824GluPhe: 3.824 ± 0.531
3.616GluGly: 3.616 ± 0.575
0.417GluHis: 0.417 ± 0.15
5.076GluIle: 5.076 ± 0.484
3.824GluLys: 3.824 ± 0.635
5.354GluLeu: 5.354 ± 0.57
2.434GluMet: 2.434 ± 0.565
2.99GluAsn: 2.99 ± 0.489
1.182GluPro: 1.182 ± 0.284
2.364GluGln: 2.364 ± 0.368
3.268GluArg: 3.268 ± 0.592
4.589GluSer: 4.589 ± 0.67
3.546GluThr: 3.546 ± 0.478
5.215GluVal: 5.215 ± 0.648
0.695GluTrp: 0.695 ± 0.223
2.295GluTyr: 2.295 ± 0.441
0.0GluXaa: 0.0 ± 0.0
Phe
2.92PheAla: 2.92 ± 0.512
0.904PheCys: 0.904 ± 0.253
3.616PheAsp: 3.616 ± 0.468
2.225PheGlu: 2.225 ± 0.456
1.043PhePhe: 1.043 ± 0.282
3.824PheGly: 3.824 ± 0.499
0.765PheHis: 0.765 ± 0.255
2.086PheIle: 2.086 ± 0.309
2.364PheLys: 2.364 ± 0.449
2.155PheLeu: 2.155 ± 0.429
1.182PheMet: 1.182 ± 0.295
2.92PheAsn: 2.92 ± 0.484
1.252PhePro: 1.252 ± 0.304
1.321PheGln: 1.321 ± 0.302
1.53PheArg: 1.53 ± 0.251
2.364PheSer: 2.364 ± 0.349
2.503PheThr: 2.503 ± 0.469
2.295PheVal: 2.295 ± 0.333
0.487PheTrp: 0.487 ± 0.184
1.321PheTyr: 1.321 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
3.963GlyAla: 3.963 ± 0.613
1.182GlyCys: 1.182 ± 0.278
5.493GlyAsp: 5.493 ± 1.314
5.215GlyGlu: 5.215 ± 0.597
3.129GlyPhe: 3.129 ± 0.857
6.536GlyGly: 6.536 ± 1.013
0.695GlyHis: 0.695 ± 0.285
6.258GlyIle: 6.258 ± 0.638
4.937GlyLys: 4.937 ± 0.718
6.258GlyLeu: 6.258 ± 0.671
2.086GlyMet: 2.086 ± 0.501
3.477GlyAsn: 3.477 ± 0.469
2.434GlyPro: 2.434 ± 1.557
1.599GlyGln: 1.599 ± 0.291
3.338GlyArg: 3.338 ± 0.375
6.188GlySer: 6.188 ± 0.603
4.589GlyThr: 4.589 ± 0.669
6.397GlyVal: 6.397 ± 0.662
1.113GlyTrp: 1.113 ± 0.265
4.311GlyTyr: 4.311 ± 0.522
0.0GlyXaa: 0.0 ± 0.0
His
0.834HisAla: 0.834 ± 0.273
0.209HisCys: 0.209 ± 0.143
0.765HisAsp: 0.765 ± 0.23
0.973HisGlu: 0.973 ± 0.278
0.556HisPhe: 0.556 ± 0.189
0.834HisGly: 0.834 ± 0.235
0.417HisHis: 0.417 ± 0.149
0.765HisIle: 0.765 ± 0.245
1.947HisLys: 1.947 ± 0.456
1.043HisLeu: 1.043 ± 0.277
0.139HisMet: 0.139 ± 0.089
0.695HisAsn: 0.695 ± 0.259
0.348HisPro: 0.348 ± 0.165
0.278HisGln: 0.278 ± 0.147
0.765HisArg: 0.765 ± 0.236
0.695HisSer: 0.695 ± 0.212
0.834HisThr: 0.834 ± 0.211
1.113HisVal: 1.113 ± 0.345
0.0HisTrp: 0.0 ± 0.0
0.348HisTyr: 0.348 ± 0.169
0.0HisXaa: 0.0 ± 0.0
Ile
5.423IleAla: 5.423 ± 0.561
1.113IleCys: 1.113 ± 0.272
5.006IleAsp: 5.006 ± 0.609
5.284IleGlu: 5.284 ± 0.739
1.877IlePhe: 1.877 ± 0.297
3.894IleGly: 3.894 ± 0.647
0.973IleHis: 0.973 ± 0.233
3.477IleIle: 3.477 ± 0.549
4.172IleLys: 4.172 ± 0.473
2.99IleLeu: 2.99 ± 0.452
1.46IleMet: 1.46 ± 0.405
3.338IleAsn: 3.338 ± 0.498
2.642IlePro: 2.642 ± 0.383
2.642IleGln: 2.642 ± 0.507
3.477IleArg: 3.477 ± 0.553
4.033IleSer: 4.033 ± 0.537
4.659IleThr: 4.659 ± 0.571
3.546IleVal: 3.546 ± 0.492
0.695IleTrp: 0.695 ± 0.244
3.129IleTyr: 3.129 ± 0.531
0.0IleXaa: 0.0 ± 0.0
Lys
6.745LysAla: 6.745 ± 0.915
0.487LysCys: 0.487 ± 0.193
3.407LysAsp: 3.407 ± 0.437
4.589LysGlu: 4.589 ± 0.909
2.573LysPhe: 2.573 ± 0.408
4.38LysGly: 4.38 ± 1.183
1.182LysHis: 1.182 ± 0.287
3.963LysIle: 3.963 ± 0.544
3.546LysLys: 3.546 ± 0.59
5.423LysLeu: 5.423 ± 0.604
2.642LysMet: 2.642 ± 0.564
1.947LysAsn: 1.947 ± 0.341
1.46LysPro: 1.46 ± 0.38
2.642LysGln: 2.642 ± 0.473
2.781LysArg: 2.781 ± 0.522
4.798LysSer: 4.798 ± 0.583
3.616LysThr: 3.616 ± 0.555
4.659LysVal: 4.659 ± 0.627
0.695LysTrp: 0.695 ± 0.193
2.99LysTyr: 2.99 ± 0.502
0.0LysXaa: 0.0 ± 0.0
Leu
6.884LeuAla: 6.884 ± 0.719
0.904LeuCys: 0.904 ± 0.307
4.45LeuAsp: 4.45 ± 0.634
3.963LeuGlu: 3.963 ± 0.441
2.225LeuPhe: 2.225 ± 0.339
3.894LeuGly: 3.894 ± 0.557
1.182LeuHis: 1.182 ± 0.286
3.755LeuIle: 3.755 ± 0.619
4.241LeuLys: 4.241 ± 0.417
4.102LeuLeu: 4.102 ± 0.557
1.808LeuMet: 1.808 ± 0.406
3.616LeuAsn: 3.616 ± 0.417
3.407LeuPro: 3.407 ± 0.48
2.99LeuGln: 2.99 ± 0.654
3.685LeuArg: 3.685 ± 0.496
6.049LeuSer: 6.049 ± 0.635
5.284LeuThr: 5.284 ± 0.707
5.006LeuVal: 5.006 ± 0.505
0.556LeuTrp: 0.556 ± 0.183
2.225LeuTyr: 2.225 ± 0.318
0.0LeuXaa: 0.0 ± 0.0
Met
3.477MetAla: 3.477 ± 0.488
0.278MetCys: 0.278 ± 0.138
0.834MetAsp: 0.834 ± 0.292
1.53MetGlu: 1.53 ± 0.387
0.973MetPhe: 0.973 ± 0.3
1.182MetGly: 1.182 ± 0.261
0.417MetHis: 0.417 ± 0.16
2.225MetIle: 2.225 ± 0.455
1.738MetLys: 1.738 ± 0.346
1.808MetLeu: 1.808 ± 0.49
1.043MetMet: 1.043 ± 0.344
1.391MetAsn: 1.391 ± 0.326
0.626MetPro: 0.626 ± 0.17
0.695MetGln: 0.695 ± 0.164
1.669MetArg: 1.669 ± 0.411
1.113MetSer: 1.113 ± 0.258
2.434MetThr: 2.434 ± 0.474
1.808MetVal: 1.808 ± 0.297
0.278MetTrp: 0.278 ± 0.143
0.487MetTyr: 0.487 ± 0.189
0.0MetXaa: 0.0 ± 0.0
Asn
3.963AsnAla: 3.963 ± 0.48
0.556AsnCys: 0.556 ± 0.282
3.198AsnAsp: 3.198 ± 0.436
3.824AsnGlu: 3.824 ± 0.605
1.113AsnPhe: 1.113 ± 0.236
5.284AsnGly: 5.284 ± 0.846
0.904AsnHis: 0.904 ± 0.33
3.268AsnIle: 3.268 ± 0.441
2.642AsnLys: 2.642 ± 0.426
3.616AsnLeu: 3.616 ± 0.486
1.113AsnMet: 1.113 ± 0.349
3.546AsnAsn: 3.546 ± 0.517
1.599AsnPro: 1.599 ± 0.314
1.738AsnGln: 1.738 ± 0.435
1.738AsnArg: 1.738 ± 0.319
4.102AsnSer: 4.102 ± 0.844
2.99AsnThr: 2.99 ± 0.444
3.059AsnVal: 3.059 ± 0.455
0.626AsnTrp: 0.626 ± 0.165
1.947AsnTyr: 1.947 ± 0.427
0.0AsnXaa: 0.0 ± 0.0
Pro
3.477ProAla: 3.477 ± 0.496
0.348ProCys: 0.348 ± 0.177
1.53ProAsp: 1.53 ± 0.434
2.642ProGlu: 2.642 ± 0.457
1.738ProPhe: 1.738 ± 0.369
2.225ProGly: 2.225 ± 0.497
0.348ProHis: 0.348 ± 0.155
1.391ProIle: 1.391 ± 0.284
1.113ProLys: 1.113 ± 0.258
1.947ProLeu: 1.947 ± 0.383
0.695ProMet: 0.695 ± 0.203
1.321ProAsn: 1.321 ± 0.274
0.834ProPro: 0.834 ± 0.271
2.712ProGln: 2.712 ± 0.983
1.391ProArg: 1.391 ± 0.271
1.808ProSer: 1.808 ± 0.397
1.46ProThr: 1.46 ± 0.384
3.338ProVal: 3.338 ± 0.505
0.417ProTrp: 0.417 ± 0.145
1.182ProTyr: 1.182 ± 0.232
0.0ProXaa: 0.0 ± 0.0
Gln
4.102GlnAla: 4.102 ± 0.657
0.556GlnCys: 0.556 ± 0.209
1.877GlnAsp: 1.877 ± 0.359
2.155GlnGlu: 2.155 ± 0.399
1.321GlnPhe: 1.321 ± 0.28
3.546GlnGly: 3.546 ± 1.667
0.348GlnHis: 0.348 ± 0.125
3.407GlnIle: 3.407 ± 0.699
2.295GlnLys: 2.295 ± 0.436
2.99GlnLeu: 2.99 ± 0.465
0.626GlnMet: 0.626 ± 0.184
1.53GlnAsn: 1.53 ± 0.319
1.46GlnPro: 1.46 ± 0.324
2.851GlnGln: 2.851 ± 0.904
1.321GlnArg: 1.321 ± 0.349
3.268GlnSer: 3.268 ± 0.472
1.53GlnThr: 1.53 ± 0.288
2.503GlnVal: 2.503 ± 0.378
0.556GlnTrp: 0.556 ± 0.187
1.391GlnTyr: 1.391 ± 0.374
0.0GlnXaa: 0.0 ± 0.0
Arg
3.129ArgAla: 3.129 ± 0.504
1.043ArgCys: 1.043 ± 0.373
2.225ArgAsp: 2.225 ± 0.369
3.268ArgGlu: 3.268 ± 0.456
2.295ArgPhe: 2.295 ± 0.313
3.129ArgGly: 3.129 ± 0.618
0.695ArgHis: 0.695 ± 0.278
2.99ArgIle: 2.99 ± 0.608
3.685ArgLys: 3.685 ± 0.55
3.755ArgLeu: 3.755 ± 0.478
0.765ArgMet: 0.765 ± 0.248
2.434ArgAsn: 2.434 ± 0.533
2.016ArgPro: 2.016 ± 0.335
2.434ArgGln: 2.434 ± 0.425
2.016ArgArg: 2.016 ± 0.285
2.851ArgSer: 2.851 ± 0.49
2.016ArgThr: 2.016 ± 0.327
3.894ArgVal: 3.894 ± 0.412
0.765ArgTrp: 0.765 ± 0.235
2.295ArgTyr: 2.295 ± 0.361
0.0ArgXaa: 0.0 ± 0.0
Ser
6.397SerAla: 6.397 ± 0.821
0.487SerCys: 0.487 ± 0.215
5.076SerAsp: 5.076 ± 0.616
4.867SerGlu: 4.867 ± 0.571
3.407SerPhe: 3.407 ± 0.53
6.466SerGly: 6.466 ± 0.822
0.626SerHis: 0.626 ± 0.177
3.407SerIle: 3.407 ± 0.6
4.728SerLys: 4.728 ± 0.633
5.215SerLeu: 5.215 ± 0.625
1.669SerMet: 1.669 ± 0.386
3.963SerAsn: 3.963 ± 0.512
2.434SerPro: 2.434 ± 0.443
3.198SerGln: 3.198 ± 0.471
2.712SerArg: 2.712 ± 0.573
5.702SerSer: 5.702 ± 1.209
3.546SerThr: 3.546 ± 0.594
5.91SerVal: 5.91 ± 0.676
0.695SerTrp: 0.695 ± 0.24
3.129SerTyr: 3.129 ± 0.551
0.0SerXaa: 0.0 ± 0.0
Thr
4.937ThrAla: 4.937 ± 0.689
0.834ThrCys: 0.834 ± 0.266
2.503ThrAsp: 2.503 ± 0.413
2.434ThrGlu: 2.434 ± 0.366
2.016ThrPhe: 2.016 ± 0.425
6.049ThrGly: 6.049 ± 0.825
0.487ThrHis: 0.487 ± 0.177
4.033ThrIle: 4.033 ± 0.421
2.851ThrLys: 2.851 ± 0.461
4.311ThrLeu: 4.311 ± 0.628
1.391ThrMet: 1.391 ± 0.33
3.407ThrAsn: 3.407 ± 0.458
2.781ThrPro: 2.781 ± 0.384
2.086ThrGln: 2.086 ± 0.324
2.225ThrArg: 2.225 ± 0.305
3.963ThrSer: 3.963 ± 0.521
3.338ThrThr: 3.338 ± 0.5
4.311ThrVal: 4.311 ± 0.544
0.765ThrTrp: 0.765 ± 0.212
2.503ThrTyr: 2.503 ± 0.438
0.0ThrXaa: 0.0 ± 0.0
Val
5.215ValAla: 5.215 ± 0.787
0.695ValCys: 0.695 ± 0.222
4.937ValAsp: 4.937 ± 0.397
4.033ValGlu: 4.033 ± 0.68
2.99ValPhe: 2.99 ± 0.442
4.867ValGly: 4.867 ± 0.611
0.973ValHis: 0.973 ± 0.285
4.102ValIle: 4.102 ± 0.511
5.354ValLys: 5.354 ± 0.813
4.798ValLeu: 4.798 ± 0.475
2.086ValMet: 2.086 ± 0.409
3.963ValAsn: 3.963 ± 0.561
2.295ValPro: 2.295 ± 0.433
2.712ValGln: 2.712 ± 0.473
4.311ValArg: 4.311 ± 0.64
6.814ValSer: 6.814 ± 0.698
3.824ValThr: 3.824 ± 0.408
5.702ValVal: 5.702 ± 0.953
0.834ValTrp: 0.834 ± 0.217
2.295ValTyr: 2.295 ± 0.34
0.0ValXaa: 0.0 ± 0.0
Trp
0.765TrpAla: 0.765 ± 0.219
0.348TrpCys: 0.348 ± 0.178
0.626TrpAsp: 0.626 ± 0.168
0.556TrpGlu: 0.556 ± 0.176
0.487TrpPhe: 0.487 ± 0.237
1.043TrpGly: 1.043 ± 0.245
0.139TrpHis: 0.139 ± 0.092
0.834TrpIle: 0.834 ± 0.221
0.765TrpLys: 0.765 ± 0.226
0.973TrpLeu: 0.973 ± 0.206
0.139TrpMet: 0.139 ± 0.083
0.278TrpAsn: 0.278 ± 0.138
0.278TrpPro: 0.278 ± 0.144
0.417TrpGln: 0.417 ± 0.215
0.973TrpArg: 0.973 ± 0.201
1.043TrpSer: 1.043 ± 0.337
0.626TrpThr: 0.626 ± 0.207
1.113TrpVal: 1.113 ± 0.274
0.278TrpTrp: 0.278 ± 0.135
0.278TrpTyr: 0.278 ± 0.122
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.877TyrAla: 1.877 ± 0.417
0.765TyrCys: 0.765 ± 0.254
2.99TyrAsp: 2.99 ± 0.467
2.295TyrGlu: 2.295 ± 0.425
1.599TyrPhe: 1.599 ± 0.36
3.129TyrGly: 3.129 ± 0.49
0.556TyrHis: 0.556 ± 0.186
2.155TyrIle: 2.155 ± 0.361
2.434TyrLys: 2.434 ± 0.45
2.086TyrLeu: 2.086 ± 0.379
1.182TyrMet: 1.182 ± 0.287
2.295TyrAsn: 2.295 ± 0.39
1.182TyrPro: 1.182 ± 0.303
1.53TyrGln: 1.53 ± 0.238
2.851TyrArg: 2.851 ± 0.512
3.198TyrSer: 3.198 ± 0.598
2.573TyrThr: 2.573 ± 0.431
2.573TyrVal: 2.573 ± 0.38
0.556TyrTrp: 0.556 ± 0.248
1.391TyrTyr: 1.391 ± 0.402
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (14383 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski