Citrus chlorotic dwarf associated virus
Average proteome isoelectric point is 7.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6X8D9|I6X8D9_9GEMI Replication-associated protein (Fragment) OS=Citrus chlorotic dwarf associated virus OX=1202142 PE=4 SV=1
MM1 pKa = 7.37 CHH3 pKa = 6.11 YY4 pKa = 10.41 ALSVQDD10 pKa = 4.22 LPEE13 pKa = 4.26 SLFGLMSMLSVRR25 pKa = 11.84 YY26 pKa = 8.35 LKK28 pKa = 10.63 CVEE31 pKa = 3.93 EE32 pKa = 5.02 RR33 pKa = 11.84 EE34 pKa = 4.35 MEE36 pKa = 4.14 RR37 pKa = 11.84 SLMVGNPVGGALGNARR53 pKa = 11.84 ILIRR57 pKa = 11.84 LIRR60 pKa = 11.84 RR61 pKa = 11.84 YY62 pKa = 9.47 CRR64 pKa = 11.84 CRR66 pKa = 11.84 DD67 pKa = 3.14 WVRR70 pKa = 11.84 KK71 pKa = 9.55 GRR73 pKa = 11.84 VNGEE77 pKa = 3.79 YY78 pKa = 10.38 QEE80 pKa = 4.47 WRR82 pKa = 11.84 TIWDD86 pKa = 4.3 KK87 pKa = 11.28 PCNDD91 pKa = 4.68 CGDD94 pKa = 4.08 AADD97 pKa = 4.27 VGHH100 pKa = 6.76 KK101 pKa = 9.21 EE102 pKa = 3.93 KK103 pKa = 11.08 KK104 pKa = 8.14 EE105 pKa = 3.78 AQVAEE110 pKa = 4.42 KK111 pKa = 10.63 GKK113 pKa = 9.84 EE114 pKa = 3.94 AQDD117 pKa = 3.4 CWGCICEE124 pKa = 5.02 GPAQEE129 pKa = 5.4 KK130 pKa = 10.29 VEE132 pKa = 4.08 QRR134 pKa = 11.84 CSSGVV139 pKa = 3.12
Molecular weight: 15.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.572
IPC2_protein 6.649
IPC_protein 6.693
Toseland 6.313
ProMoST 7.205
Dawson 7.337
Bjellqvist 7.468
Wikipedia 7.234
Rodwell 7.337
Grimsley 6.3
Solomon 7.38
Lehninger 7.41
Nozaki 7.892
DTASelect 7.497
Thurlkill 7.541
EMBOSS 7.571
Sillero 7.878
Patrickios 4.037
IPC_peptide 7.38
IPC2_peptide 7.263
IPC2.peptide.svr19 7.094
Protein with the highest isoelectric point:
>tr|I6XMA9|I6XMA9_9GEMI Movement protein BC1 OS=Citrus chlorotic dwarf associated virus OX=1202142 PE=3 SV=1
MM1 pKa = 7.27 VSTRR5 pKa = 11.84 SGGQYY10 pKa = 7.93 GTSRR14 pKa = 11.84 VTTVEE19 pKa = 4.29 TLPMWATKK27 pKa = 9.65 RR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 PRR33 pKa = 11.84 WPRR36 pKa = 11.84 KK37 pKa = 7.42 EE38 pKa = 4.19 KK39 pKa = 10.34 KK40 pKa = 9.58 PKK42 pKa = 8.88 IAGAVFVKK50 pKa = 10.33 ARR52 pKa = 11.84 PRR54 pKa = 11.84 RR55 pKa = 11.84 KK56 pKa = 9.35 SSKK59 pKa = 10.14 GVPPGCKK66 pKa = 9.88 GPCKK70 pKa = 9.45 THH72 pKa = 5.31 TVDD75 pKa = 3.77 VIKK78 pKa = 9.89 TIYY81 pKa = 10.0 HH82 pKa = 7.04 DD83 pKa = 3.92 GRR85 pKa = 11.84 GSGMISNIDD94 pKa = 3.42 RR95 pKa = 11.84 GDD97 pKa = 3.52 EE98 pKa = 3.84 LGQRR102 pKa = 11.84 EE103 pKa = 4.13 GRR105 pKa = 11.84 KK106 pKa = 9.19 IRR108 pKa = 11.84 VSRR111 pKa = 11.84 MIIRR115 pKa = 11.84 GKK117 pKa = 9.83 IWLDD121 pKa = 3.25 VNNASVPGSNLAKK134 pKa = 10.0 IWIFKK139 pKa = 9.87 DD140 pKa = 3.25 RR141 pKa = 11.84 RR142 pKa = 11.84 PGTEE146 pKa = 3.16 PVAFNALMDD155 pKa = 4.0 MSDD158 pKa = 4.42 SEE160 pKa = 4.32 PLSAFVKK167 pKa = 9.91 VDD169 pKa = 3.05 YY170 pKa = 10.25 RR171 pKa = 11.84 DD172 pKa = 3.29 RR173 pKa = 11.84 FIALHH178 pKa = 5.79 TMTVDD183 pKa = 3.0 LHH185 pKa = 6.18 GGKK188 pKa = 10.07 DD189 pKa = 3.64 FRR191 pKa = 11.84 VDD193 pKa = 3.82 EE194 pKa = 4.86 LDD196 pKa = 3.57 LDD198 pKa = 4.05 EE199 pKa = 5.33 LVEE202 pKa = 4.28 INSDD206 pKa = 3.64 VLFSHH211 pKa = 7.09 EE212 pKa = 4.67 DD213 pKa = 3.56 DD214 pKa = 4.3 GSVAHH219 pKa = 7.03 TIQNGIFIYY228 pKa = 8.78 YY229 pKa = 9.6 ACSDD233 pKa = 3.54 PRR235 pKa = 11.84 QTVQITAQARR245 pKa = 11.84 LYY247 pKa = 10.75 FYY249 pKa = 10.96 DD250 pKa = 3.54 STSNN254 pKa = 3.23
Molecular weight: 28.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.068
IPC2_protein 9.048
IPC_protein 9.048
Toseland 9.853
ProMoST 9.516
Dawson 10.058
Bjellqvist 9.721
Wikipedia 10.204
Rodwell 10.423
Grimsley 10.116
Solomon 10.087
Lehninger 10.058
Nozaki 9.897
DTASelect 9.706
Thurlkill 9.911
EMBOSS 10.262
Sillero 9.984
Patrickios 9.882
IPC_peptide 10.087
IPC2_peptide 8.346
IPC2.peptide.svr19 7.968
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1103
135
306
220.6
25.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.074 ± 0.44
3.173 ± 0.867
6.346 ± 0.774
5.621 ± 1.078
3.626 ± 0.737
5.984 ± 1.082
2.539 ± 0.327
5.44 ± 0.767
6.346 ± 0.288
6.981 ± 0.65
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.539 ± 0.306
4.17 ± 0.448
5.802 ± 0.745
3.717 ± 0.408
7.525 ± 0.869
8.432 ± 1.075
3.989 ± 0.891
6.437 ± 0.766
1.813 ± 0.265
3.445 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here