Amino acid dipepetide frequency for bacterium HR38

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.507AlaAla: 8.507 ± 0.362
0.805AlaCys: 0.805 ± 0.094
2.644AlaAsp: 2.644 ± 0.153
7.875AlaGlu: 7.875 ± 0.302
5.24AlaPhe: 5.24 ± 0.246
8.152AlaGly: 8.152 ± 0.251
2.549AlaHis: 2.549 ± 0.16
3.527AlaIle: 3.527 ± 0.216
4.096AlaLys: 4.096 ± 0.185
20.659AlaLeu: 20.659 ± 0.584
2.083AlaMet: 2.083 ± 0.137
1.113AlaAsn: 1.113 ± 0.102
4.861AlaPro: 4.861 ± 0.202
4.395AlaGln: 4.395 ± 0.217
9.217AlaArg: 9.217 ± 0.339
4.253AlaSer: 4.253 ± 0.21
3.961AlaThr: 3.961 ± 0.193
6.526AlaVal: 6.526 ± 0.239
2.06AlaTrp: 2.06 ± 0.135
3.504AlaTyr: 3.504 ± 0.22
0.0AlaXaa: 0.0 ± 0.0
Cys
0.339CysAla: 0.339 ± 0.047
0.047CysCys: 0.047 ± 0.019
0.15CysAsp: 0.15 ± 0.039
0.181CysGlu: 0.181 ± 0.041
0.158CysPhe: 0.158 ± 0.034
0.663CysGly: 0.663 ± 0.092
0.221CysHis: 0.221 ± 0.053
0.15CysIle: 0.15 ± 0.034
0.142CysLys: 0.142 ± 0.04
0.481CysLeu: 0.481 ± 0.066
0.063CysMet: 0.063 ± 0.026
0.087CysAsn: 0.087 ± 0.025
0.623CysPro: 0.623 ± 0.086
0.181CysGln: 0.181 ± 0.039
0.395CysArg: 0.395 ± 0.06
0.197CysSer: 0.197 ± 0.047
0.276CysThr: 0.276 ± 0.042
0.245CysVal: 0.245 ± 0.059
0.079CysTrp: 0.079 ± 0.023
0.174CysTyr: 0.174 ± 0.042
0.0CysXaa: 0.0 ± 0.0
Asp
2.841AspAla: 2.841 ± 0.156
0.15AspCys: 0.15 ± 0.031
0.971AspAsp: 0.971 ± 0.097
2.194AspGlu: 2.194 ± 0.141
1.428AspPhe: 1.428 ± 0.098
3.007AspGly: 3.007 ± 0.182
0.971AspHis: 0.971 ± 0.108
1.121AspIle: 1.121 ± 0.114
0.844AspLys: 0.844 ± 0.099
6.447AspLeu: 6.447 ± 0.222
0.45AspMet: 0.45 ± 0.07
0.41AspAsn: 0.41 ± 0.063
4.301AspPro: 4.301 ± 0.222
1.026AspGln: 1.026 ± 0.094
2.833AspArg: 2.833 ± 0.166
0.931AspSer: 0.931 ± 0.084
1.31AspThr: 1.31 ± 0.102
2.123AspVal: 2.123 ± 0.147
0.608AspTrp: 0.608 ± 0.072
1.105AspTyr: 1.105 ± 0.094
0.0AspXaa: 0.0 ± 0.0
Glu
13.541GluAla: 13.541 ± 0.465
0.118GluCys: 0.118 ± 0.032
3.046GluAsp: 3.046 ± 0.158
9.343GluGlu: 9.343 ± 0.412
1.815GluPhe: 1.815 ± 0.12
9.975GluGly: 9.975 ± 0.314
0.923GluHis: 0.923 ± 0.088
2.502GluIle: 2.502 ± 0.194
3.598GluLys: 3.598 ± 0.207
7.781GluLeu: 7.781 ± 0.31
1.357GluMet: 1.357 ± 0.117
1.618GluAsn: 1.618 ± 0.115
3.259GluPro: 3.259 ± 0.16
0.955GluGln: 0.955 ± 0.097
6.976GluArg: 6.976 ± 0.322
2.02GluSer: 2.02 ± 0.122
2.604GluThr: 2.604 ± 0.14
8.467GluVal: 8.467 ± 0.32
0.971GluTrp: 0.971 ± 0.08
1.326GluTyr: 1.326 ± 0.108
0.0GluXaa: 0.0 ± 0.0
Phe
3.977PheAla: 3.977 ± 0.209
0.189PheCys: 0.189 ± 0.039
1.152PheAsp: 1.152 ± 0.1
1.531PheGlu: 1.531 ± 0.115
1.925PhePhe: 1.925 ± 0.159
3.575PheGly: 3.575 ± 0.182
1.065PheHis: 1.065 ± 0.103
0.979PheIle: 0.979 ± 0.097
0.963PheLys: 0.963 ± 0.085
7.0PheLeu: 7.0 ± 0.299
0.481PheMet: 0.481 ± 0.06
0.663PheAsn: 0.663 ± 0.083
3.046PhePro: 3.046 ± 0.162
1.649PheGln: 1.649 ± 0.128
3.291PheArg: 3.291 ± 0.137
1.933PheSer: 1.933 ± 0.149
1.602PheThr: 1.602 ± 0.125
2.336PheVal: 2.336 ± 0.172
0.71PheTrp: 0.71 ± 0.086
1.397PheTyr: 1.397 ± 0.107
0.0PheXaa: 0.0 ± 0.0
Gly
7.939GlyAla: 7.939 ± 0.238
0.473GlyCys: 0.473 ± 0.068
3.038GlyAsp: 3.038 ± 0.206
8.404GlyGlu: 8.404 ± 0.223
4.522GlyPhe: 4.522 ± 0.19
7.986GlyGly: 7.986 ± 0.351
2.052GlyHis: 2.052 ± 0.181
3.472GlyIle: 3.472 ± 0.185
4.056GlyLys: 4.056 ± 0.196
14.591GlyLeu: 14.591 ± 0.471
1.996GlyMet: 1.996 ± 0.138
1.657GlyAsn: 1.657 ± 0.11
4.64GlyPro: 4.64 ± 0.181
3.472GlyGln: 3.472 ± 0.235
6.786GlyArg: 6.786 ± 0.257
3.717GlySer: 3.717 ± 0.182
3.725GlyThr: 3.725 ± 0.179
8.68GlyVal: 8.68 ± 0.244
1.16GlyTrp: 1.16 ± 0.102
3.093GlyTyr: 3.093 ± 0.154
0.0GlyXaa: 0.0 ± 0.0
His
1.847HisAla: 1.847 ± 0.141
0.11HisCys: 0.11 ± 0.028
0.6HisAsp: 0.6 ± 0.055
0.939HisGlu: 0.939 ± 0.101
0.702HisPhe: 0.702 ± 0.074
1.823HisGly: 1.823 ± 0.139
0.773HisHis: 0.773 ± 0.122
0.505HisIle: 0.505 ± 0.069
0.434HisLys: 0.434 ± 0.058
3.717HisLeu: 3.717 ± 0.206
0.268HisMet: 0.268 ± 0.046
0.268HisAsn: 0.268 ± 0.046
2.233HisPro: 2.233 ± 0.169
0.797HisGln: 0.797 ± 0.103
1.918HisArg: 1.918 ± 0.157
0.568HisSer: 0.568 ± 0.053
0.884HisThr: 0.884 ± 0.079
1.247HisVal: 1.247 ± 0.11
0.284HisTrp: 0.284 ± 0.045
0.616HisTyr: 0.616 ± 0.074
0.0HisXaa: 0.0 ± 0.0
Ile
2.999IleAla: 2.999 ± 0.181
0.158IleCys: 0.158 ± 0.034
1.057IleAsp: 1.057 ± 0.116
1.515IleGlu: 1.515 ± 0.136
0.923IlePhe: 0.923 ± 0.102
2.494IleGly: 2.494 ± 0.159
0.639IleHis: 0.639 ± 0.068
0.758IleIle: 0.758 ± 0.103
0.947IleLys: 0.947 ± 0.113
5.137IleLeu: 5.137 ± 0.264
0.434IleMet: 0.434 ± 0.062
0.56IleAsn: 0.56 ± 0.088
2.628IlePro: 2.628 ± 0.145
1.192IleGln: 1.192 ± 0.101
2.344IleArg: 2.344 ± 0.148
1.231IleSer: 1.231 ± 0.104
1.326IleThr: 1.326 ± 0.11
1.878IleVal: 1.878 ± 0.182
0.347IleTrp: 0.347 ± 0.054
0.852IleTyr: 0.852 ± 0.086
0.0IleXaa: 0.0 ± 0.0
Lys
5.587LysAla: 5.587 ± 0.215
0.134LysCys: 0.134 ± 0.044
2.162LysAsp: 2.162 ± 0.145
4.285LysGlu: 4.285 ± 0.215
0.434LysPhe: 0.434 ± 0.074
4.435LysGly: 4.435 ± 0.196
0.379LysHis: 0.379 ± 0.054
0.805LysIle: 0.805 ± 0.093
1.76LysLys: 1.76 ± 0.149
2.975LysLeu: 2.975 ± 0.179
0.481LysMet: 0.481 ± 0.068
0.655LysAsn: 0.655 ± 0.07
2.281LysPro: 2.281 ± 0.15
0.639LysGln: 0.639 ± 0.072
2.967LysArg: 2.967 ± 0.164
1.128LysSer: 1.128 ± 0.098
1.602LysThr: 1.602 ± 0.112
3.125LysVal: 3.125 ± 0.161
0.253LysTrp: 0.253 ± 0.049
0.489LysTyr: 0.489 ± 0.063
0.0LysXaa: 0.0 ± 0.0
Leu
17.392LeuAla: 17.392 ± 0.514
0.616LeuCys: 0.616 ± 0.074
5.595LeuAsp: 5.595 ± 0.241
13.155LeuGlu: 13.155 ± 0.428
5.761LeuPhe: 5.761 ± 0.311
14.496LeuGly: 14.496 ± 0.494
2.683LeuHis: 2.683 ± 0.185
3.591LeuIle: 3.591 ± 0.198
6.66LeuLys: 6.66 ± 0.278
21.433LeuLeu: 21.433 ± 0.755
1.815LeuMet: 1.815 ± 0.145
2.241LeuAsn: 2.241 ± 0.146
9.509LeuPro: 9.509 ± 0.31
3.654LeuGln: 3.654 ± 0.18
10.393LeuArg: 10.393 ± 0.359
8.452LeuSer: 8.452 ± 0.31
5.405LeuThr: 5.405 ± 0.24
9.714LeuVal: 9.714 ± 0.353
2.146LeuTrp: 2.146 ± 0.145
3.819LeuTyr: 3.819 ± 0.181
0.0LeuXaa: 0.0 ± 0.0
Met
2.407MetAla: 2.407 ± 0.128
0.032MetCys: 0.032 ± 0.016
0.758MetAsp: 0.758 ± 0.072
1.665MetGlu: 1.665 ± 0.113
0.339MetPhe: 0.339 ± 0.053
1.918MetGly: 1.918 ± 0.123
0.292MetHis: 0.292 ± 0.043
0.418MetIle: 0.418 ± 0.054
0.734MetLys: 0.734 ± 0.069
1.349MetLeu: 1.349 ± 0.115
0.268MetMet: 0.268 ± 0.051
0.308MetAsn: 0.308 ± 0.052
0.836MetPro: 0.836 ± 0.082
0.268MetGln: 0.268 ± 0.054
1.894MetArg: 1.894 ± 0.129
0.544MetSer: 0.544 ± 0.07
0.552MetThr: 0.552 ± 0.063
1.57MetVal: 1.57 ± 0.113
0.197MetTrp: 0.197 ± 0.042
0.276MetTyr: 0.276 ± 0.049
0.0MetXaa: 0.0 ± 0.0
Asn
1.697AsnAla: 1.697 ± 0.124
0.103AsnCys: 0.103 ± 0.028
0.489AsnAsp: 0.489 ± 0.061
0.592AsnGlu: 0.592 ± 0.067
0.616AsnPhe: 0.616 ± 0.075
1.097AsnGly: 1.097 ± 0.098
0.276AsnHis: 0.276 ± 0.051
0.608AsnIle: 0.608 ± 0.074
0.426AsnLys: 0.426 ± 0.065
3.054AsnLeu: 3.054 ± 0.191
0.134AsnMet: 0.134 ± 0.031
0.363AsnAsn: 0.363 ± 0.057
2.186AsnPro: 2.186 ± 0.134
0.442AsnGln: 0.442 ± 0.068
1.326AsnArg: 1.326 ± 0.098
0.316AsnSer: 0.316 ± 0.051
0.789AsnThr: 0.789 ± 0.094
0.939AsnVal: 0.939 ± 0.096
0.205AsnTrp: 0.205 ± 0.043
0.395AsnTyr: 0.395 ± 0.06
0.0AsnXaa: 0.0 ± 0.0
Pro
4.522ProAla: 4.522 ± 0.175
0.466ProCys: 0.466 ± 0.084
2.478ProAsp: 2.478 ± 0.131
6.534ProGlu: 6.534 ± 0.227
3.346ProPhe: 3.346 ± 0.175
6.281ProGly: 6.281 ± 0.273
1.547ProHis: 1.547 ± 0.117
1.791ProIle: 1.791 ± 0.116
3.007ProLys: 3.007 ± 0.146
8.815ProLeu: 8.815 ± 0.301
1.334ProMet: 1.334 ± 0.09
0.758ProAsn: 0.758 ± 0.091
4.53ProPro: 4.53 ± 0.283
2.154ProGln: 2.154 ± 0.149
3.922ProArg: 3.922 ± 0.18
2.825ProSer: 2.825 ± 0.175
2.225ProThr: 2.225 ± 0.15
4.735ProVal: 4.735 ± 0.202
1.444ProTrp: 1.444 ± 0.12
2.028ProTyr: 2.028 ± 0.135
0.0ProXaa: 0.0 ± 0.0
Gln
5.216GlnAla: 5.216 ± 0.251
0.087GlnCys: 0.087 ± 0.024
1.586GlnAsp: 1.586 ± 0.121
3.496GlnGlu: 3.496 ± 0.207
0.6GlnPhe: 0.6 ± 0.078
4.64GlnGly: 4.64 ± 0.223
0.481GlnHis: 0.481 ± 0.065
0.773GlnIle: 0.773 ± 0.078
1.121GlnLys: 1.121 ± 0.089
2.012GlnLeu: 2.012 ± 0.14
0.466GlnMet: 0.466 ± 0.059
0.75GlnAsn: 0.75 ± 0.079
0.947GlnPro: 0.947 ± 0.08
0.505GlnGln: 0.505 ± 0.066
1.823GlnArg: 1.823 ± 0.142
1.002GlnSer: 1.002 ± 0.089
0.971GlnThr: 0.971 ± 0.098
3.37GlnVal: 3.37 ± 0.18
0.166GlnTrp: 0.166 ± 0.033
0.497GlnTyr: 0.497 ± 0.059
0.0GlnXaa: 0.0 ± 0.0
Arg
7.528ArgAla: 7.528 ± 0.28
0.331ArgCys: 0.331 ± 0.057
2.257ArgAsp: 2.257 ± 0.161
7.047ArgGlu: 7.047 ± 0.333
3.685ArgPhe: 3.685 ± 0.17
6.029ArgGly: 6.029 ± 0.189
1.633ArgHis: 1.633 ± 0.122
3.33ArgIle: 3.33 ± 0.161
2.857ArgLys: 2.857 ± 0.172
11.513ArgLeu: 11.513 ± 0.387
1.61ArgMet: 1.61 ± 0.112
1.136ArgAsn: 1.136 ± 0.087
4.301ArgPro: 4.301 ± 0.165
2.486ArgGln: 2.486 ± 0.14
5.926ArgArg: 5.926 ± 0.265
2.557ArgSer: 2.557 ± 0.146
2.857ArgThr: 2.857 ± 0.144
6.715ArgVal: 6.715 ± 0.233
0.907ArgTrp: 0.907 ± 0.093
2.817ArgTyr: 2.817 ± 0.151
0.0ArgXaa: 0.0 ± 0.0
Ser
3.275SerAla: 3.275 ± 0.165
0.245SerCys: 0.245 ± 0.04
1.192SerAsp: 1.192 ± 0.111
2.115SerGlu: 2.115 ± 0.13
1.839SerPhe: 1.839 ± 0.148
3.575SerGly: 3.575 ± 0.176
0.789SerHis: 0.789 ± 0.081
0.986SerIle: 0.986 ± 0.09
0.884SerLys: 0.884 ± 0.096
7.126SerLeu: 7.126 ± 0.292
0.852SerMet: 0.852 ± 0.081
0.623SerAsn: 0.623 ± 0.079
3.669SerPro: 3.669 ± 0.188
1.531SerGln: 1.531 ± 0.112
2.904SerArg: 2.904 ± 0.186
1.523SerSer: 1.523 ± 0.127
1.641SerThr: 1.641 ± 0.125
2.399SerVal: 2.399 ± 0.152
0.702SerTrp: 0.702 ± 0.079
1.026SerTyr: 1.026 ± 0.079
0.0SerXaa: 0.0 ± 0.0
Thr
2.999ThrAla: 2.999 ± 0.173
0.229ThrCys: 0.229 ± 0.044
1.531ThrAsp: 1.531 ± 0.104
1.996ThrGlu: 1.996 ± 0.136
1.618ThrPhe: 1.618 ± 0.13
4.159ThrGly: 4.159 ± 0.203
0.868ThrHis: 0.868 ± 0.083
0.829ThrIle: 0.829 ± 0.117
0.789ThrLys: 0.789 ± 0.088
6.755ThrLeu: 6.755 ± 0.287
0.434ThrMet: 0.434 ± 0.065
0.552ThrAsn: 0.552 ± 0.069
3.504ThrPro: 3.504 ± 0.177
1.057ThrGln: 1.057 ± 0.109
2.517ThrArg: 2.517 ± 0.15
1.468ThrSer: 1.468 ± 0.112
1.673ThrThr: 1.673 ± 0.161
2.975ThrVal: 2.975 ± 0.171
0.687ThrTrp: 0.687 ± 0.078
1.223ThrTyr: 1.223 ± 0.1
0.0ThrXaa: 0.0 ± 0.0
Val
8.68ValAla: 8.68 ± 0.271
0.331ValCys: 0.331 ± 0.067
2.928ValAsp: 2.928 ± 0.146
6.471ValGlu: 6.471 ± 0.265
3.046ValPhe: 3.046 ± 0.173
6.96ValGly: 6.96 ± 0.27
1.562ValHis: 1.562 ± 0.12
2.217ValIle: 2.217 ± 0.155
2.257ValLys: 2.257 ± 0.147
10.866ValLeu: 10.866 ± 0.325
1.46ValMet: 1.46 ± 0.111
1.452ValAsn: 1.452 ± 0.11
4.214ValPro: 4.214 ± 0.186
2.375ValGln: 2.375 ± 0.126
6.116ValArg: 6.116 ± 0.296
2.73ValSer: 2.73 ± 0.132
2.249ValThr: 2.249 ± 0.135
7.071ValVal: 7.071 ± 0.302
1.105ValTrp: 1.105 ± 0.09
2.517ValTyr: 2.517 ± 0.113
0.0ValXaa: 0.0 ± 0.0
Trp
1.799TrpAla: 1.799 ± 0.132
0.047TrpCys: 0.047 ± 0.019
0.521TrpAsp: 0.521 ± 0.064
1.168TrpGlu: 1.168 ± 0.106
0.371TrpPhe: 0.371 ± 0.06
1.405TrpGly: 1.405 ± 0.111
0.284TrpHis: 0.284 ± 0.045
0.481TrpIle: 0.481 ± 0.054
0.45TrpLys: 0.45 ± 0.057
1.815TrpLeu: 1.815 ± 0.123
0.355TrpMet: 0.355 ± 0.058
0.363TrpAsn: 0.363 ± 0.063
0.971TrpPro: 0.971 ± 0.102
0.663TrpGln: 0.663 ± 0.072
1.286TrpArg: 1.286 ± 0.109
0.521TrpSer: 0.521 ± 0.073
0.639TrpThr: 0.639 ± 0.08
1.105TrpVal: 1.105 ± 0.097
0.221TrpTrp: 0.221 ± 0.042
0.316TrpTyr: 0.316 ± 0.051
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.936TyrAla: 2.936 ± 0.173
0.181TyrCys: 0.181 ± 0.04
0.915TyrAsp: 0.915 ± 0.081
1.689TyrGlu: 1.689 ± 0.142
1.097TyrPhe: 1.097 ± 0.098
2.793TyrGly: 2.793 ± 0.153
0.544TyrHis: 0.544 ± 0.06
0.576TyrIle: 0.576 ± 0.057
0.552TyrLys: 0.552 ± 0.061
5.003TyrLeu: 5.003 ± 0.235
0.276TyrMet: 0.276 ± 0.042
0.473TyrAsn: 0.473 ± 0.067
2.202TyrPro: 2.202 ± 0.143
1.065TyrGln: 1.065 ± 0.098
2.691TyrArg: 2.691 ± 0.152
0.979TyrSer: 0.979 ± 0.094
1.57TyrThr: 1.57 ± 0.103
1.397TyrVal: 1.397 ± 0.11
0.529TyrTrp: 0.529 ± 0.059
0.813TyrTyr: 0.813 ± 0.097
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 485 proteins (126724 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski