Human gut gokushovirus

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; unclassified Gokushovirinae

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9Q0X9|A0A1X9Q0X9_9VIRU Putative VP1 OS=Human gut gokushovirus OX=1986031 PE=3 SV=1
MM1 pKa = 7.37NKK3 pKa = 9.69RR4 pKa = 11.84YY5 pKa = 10.12EE6 pKa = 4.15EE7 pKa = 4.1GRR9 pKa = 11.84EE10 pKa = 3.91PFFSEE15 pKa = 3.91SGEE18 pKa = 4.2KK19 pKa = 9.89FRR21 pKa = 11.84KK22 pKa = 8.98QYY24 pKa = 10.61VWTKK28 pKa = 10.67DD29 pKa = 3.35EE30 pKa = 4.07QGQEE34 pKa = 4.25VLQEE38 pKa = 3.91TAPIDD43 pKa = 3.37IQQEE47 pKa = 3.96IEE49 pKa = 4.6SYY51 pKa = 11.36AEE53 pKa = 3.78EE54 pKa = 4.7CDD56 pKa = 2.99IKK58 pKa = 11.3SIVRR62 pKa = 11.84KK63 pKa = 10.09ASFDD67 pKa = 3.67PQFLKK72 pKa = 10.93SLSEE76 pKa = 3.91GAMTGEE82 pKa = 4.18EE83 pKa = 3.83VDD85 pKa = 3.21ITEE88 pKa = 4.85FPQNIHH94 pKa = 6.34EE95 pKa = 4.31YY96 pKa = 10.49HH97 pKa = 6.87RR98 pKa = 11.84MIATAQANAMKK109 pKa = 10.67LEE111 pKa = 4.05EE112 pKa = 4.23LQKK115 pKa = 10.92KK116 pKa = 9.07AAEE119 pKa = 4.03EE120 pKa = 3.95AKK122 pKa = 9.99TEE124 pKa = 4.19SEE126 pKa = 4.07TKK128 pKa = 9.92EE129 pKa = 3.92AEE131 pKa = 4.18KK132 pKa = 10.99

Molecular weight: 15.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9Q0X5|A0A1X9Q0X5_9VIRU Peptidase M15 OS=Human gut gokushovirus OX=1986031 PE=4 SV=1
MM1 pKa = 7.48SCYY4 pKa = 10.22KK5 pKa = 10.36PLIRR9 pKa = 11.84LYY11 pKa = 10.75NPNDD15 pKa = 3.53KK16 pKa = 10.36EE17 pKa = 3.92ISGRR21 pKa = 11.84VYY23 pKa = 10.31SLSRR27 pKa = 11.84FSQLAGKK34 pKa = 7.37QLKK37 pKa = 10.34YY38 pKa = 10.6EE39 pKa = 4.03DD40 pKa = 4.56LMYY43 pKa = 10.69KK44 pKa = 9.86KK45 pKa = 10.34NVMLIPCGQCIGCRR59 pKa = 11.84IRR61 pKa = 11.84QRR63 pKa = 11.84EE64 pKa = 4.0DD65 pKa = 2.26WTTRR69 pKa = 11.84IEE71 pKa = 4.51LEE73 pKa = 3.75ARR75 pKa = 11.84DD76 pKa = 4.0YY77 pKa = 11.23PKK79 pKa = 10.6EE80 pKa = 3.92QVWFITLTYY89 pKa = 10.91DD90 pKa = 3.88DD91 pKa = 4.65DD92 pKa = 4.54HH93 pKa = 8.55VPGMIINTGEE103 pKa = 3.91IMRR106 pKa = 11.84KK107 pKa = 7.5VQYY110 pKa = 9.75VWRR113 pKa = 11.84PGEE116 pKa = 3.89KK117 pKa = 9.53RR118 pKa = 11.84PEE120 pKa = 4.07SVQTLLYY127 pKa = 10.51TDD129 pKa = 3.04IQKK132 pKa = 10.17FLKK135 pKa = 10.14RR136 pKa = 11.84LRR138 pKa = 11.84KK139 pKa = 9.69AYY141 pKa = 9.94GGKK144 pKa = 9.63LRR146 pKa = 11.84YY147 pKa = 8.78FVAGEE152 pKa = 4.04YY153 pKa = 10.92GEE155 pKa = 4.36QTARR159 pKa = 11.84PHH161 pKa = 4.93YY162 pKa = 10.8HH163 pKa = 6.27MILYY167 pKa = 8.99GWQPTDD173 pKa = 4.23LEE175 pKa = 4.28HH176 pKa = 7.5LYY178 pKa = 10.5KK179 pKa = 10.14IQHH182 pKa = 6.03NGYY185 pKa = 7.86FTSKK189 pKa = 9.62WLEE192 pKa = 4.19DD193 pKa = 3.66LWGMGQIQIAQAVPEE208 pKa = 4.37TYY210 pKa = 9.7RR211 pKa = 11.84YY212 pKa = 8.61VAGYY216 pKa = 7.14VTKK219 pKa = 10.66KK220 pKa = 9.59MYY222 pKa = 10.72EE223 pKa = 3.63IDD225 pKa = 3.75GQKK228 pKa = 10.9ANAYY232 pKa = 9.87YY233 pKa = 10.54EE234 pKa = 4.35LGQTKK239 pKa = 9.87PFACMSLKK247 pKa = 10.53PGLGDD252 pKa = 4.31HH253 pKa = 7.19YY254 pKa = 10.28YY255 pKa = 10.16QEE257 pKa = 4.59HH258 pKa = 6.52KK259 pKa = 11.28AEE261 pKa = 4.14IWRR264 pKa = 11.84QGYY267 pKa = 7.72IQCTNGKK274 pKa = 8.15HH275 pKa = 5.09AQIPRR280 pKa = 11.84YY281 pKa = 7.47YY282 pKa = 9.34EE283 pKa = 3.81KK284 pKa = 10.57MMEE287 pKa = 4.21AEE289 pKa = 4.03NPQRR293 pKa = 11.84LWKK296 pKa = 10.3IKK298 pKa = 8.01QNRR301 pKa = 11.84QAAAIAEE308 pKa = 4.05NRR310 pKa = 11.84LKK312 pKa = 11.21YY313 pKa = 10.68EE314 pKa = 3.84NTDD317 pKa = 3.45FAEE320 pKa = 4.0QCKK323 pKa = 9.23TKK325 pKa = 10.54EE326 pKa = 3.92RR327 pKa = 11.84VIKK330 pKa = 10.55KK331 pKa = 8.58QMKK334 pKa = 10.18KK335 pKa = 10.39KK336 pKa = 9.42GTLL339 pKa = 3.25

Molecular weight: 40.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1434

130

544

286.8

32.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.741 ± 0.974

0.976 ± 0.363

4.533 ± 0.509

7.322 ± 1.556

3.347 ± 0.629

8.159 ± 1.384

1.743 ± 0.29

5.649 ± 0.846

7.462 ± 1.096

5.509 ± 0.602

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.208 ± 0.375

5.021 ± 0.774

3.975 ± 0.678

5.858 ± 1.176

4.742 ± 0.854

5.788 ± 1.711

6.206 ± 0.634

5.439 ± 1.17

1.674 ± 0.254

5.649 ± 0.849
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski