Amino acid dipepetide frequency for Microbacterium phage Gelo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.505AlaAla: 11.505 ± 1.309
0.533AlaCys: 0.533 ± 0.243
4.648AlaAsp: 4.648 ± 0.587
5.79AlaGlu: 5.79 ± 0.658
2.819AlaPhe: 2.819 ± 0.521
7.695AlaGly: 7.695 ± 1.143
1.981AlaHis: 1.981 ± 0.337
5.333AlaIle: 5.333 ± 0.996
4.952AlaLys: 4.952 ± 0.727
8.457AlaLeu: 8.457 ± 0.982
2.438AlaMet: 2.438 ± 0.456
3.352AlaAsn: 3.352 ± 0.464
3.962AlaPro: 3.962 ± 0.652
4.876AlaGln: 4.876 ± 0.65
5.79AlaArg: 5.79 ± 0.916
5.486AlaSer: 5.486 ± 0.72
6.4AlaThr: 6.4 ± 0.641
6.857AlaVal: 6.857 ± 0.613
1.829AlaTrp: 1.829 ± 0.406
2.59AlaTyr: 2.59 ± 0.618
0.0AlaXaa: 0.0 ± 0.0
Cys
0.457CysAla: 0.457 ± 0.156
0.0CysCys: 0.0 ± 0.0
0.229CysAsp: 0.229 ± 0.126
0.152CysGlu: 0.152 ± 0.111
0.229CysPhe: 0.229 ± 0.122
0.762CysGly: 0.762 ± 0.28
0.305CysHis: 0.305 ± 0.146
0.0CysIle: 0.0 ± 0.0
0.533CysLys: 0.533 ± 0.2
0.686CysLeu: 0.686 ± 0.288
0.0CysMet: 0.0 ± 0.0
0.229CysAsn: 0.229 ± 0.127
0.533CysPro: 0.533 ± 0.223
0.0CysGln: 0.0 ± 0.0
0.305CysArg: 0.305 ± 0.158
0.457CysSer: 0.457 ± 0.222
0.305CysThr: 0.305 ± 0.151
0.457CysVal: 0.457 ± 0.172
0.152CysTrp: 0.152 ± 0.097
0.381CysTyr: 0.381 ± 0.188
0.0CysXaa: 0.0 ± 0.0
Asp
5.029AspAla: 5.029 ± 0.66
0.61AspCys: 0.61 ± 0.215
5.333AspAsp: 5.333 ± 0.827
6.095AspGlu: 6.095 ± 1.348
2.362AspPhe: 2.362 ± 0.413
4.267AspGly: 4.267 ± 0.656
1.143AspHis: 1.143 ± 0.26
3.429AspIle: 3.429 ± 0.437
2.438AspLys: 2.438 ± 0.449
5.562AspLeu: 5.562 ± 0.713
1.371AspMet: 1.371 ± 0.346
1.524AspAsn: 1.524 ± 0.369
4.419AspPro: 4.419 ± 0.612
2.133AspGln: 2.133 ± 0.362
3.429AspArg: 3.429 ± 0.538
3.429AspSer: 3.429 ± 0.445
2.819AspThr: 2.819 ± 0.513
4.724AspVal: 4.724 ± 0.698
2.21AspTrp: 2.21 ± 0.397
2.667AspTyr: 2.667 ± 0.488
0.0AspXaa: 0.0 ± 0.0
Glu
7.848GluAla: 7.848 ± 0.747
0.229GluCys: 0.229 ± 0.156
5.181GluAsp: 5.181 ± 1.288
5.638GluGlu: 5.638 ± 1.169
2.362GluPhe: 2.362 ± 0.405
3.962GluGly: 3.962 ± 0.63
0.99GluHis: 0.99 ± 0.277
2.895GluIle: 2.895 ± 0.472
2.362GluLys: 2.362 ± 0.43
5.333GluLeu: 5.333 ± 0.712
2.438GluMet: 2.438 ± 0.547
1.905GluAsn: 1.905 ± 0.404
2.895GluPro: 2.895 ± 0.617
2.667GluGln: 2.667 ± 0.504
3.581GluArg: 3.581 ± 0.564
2.895GluSer: 2.895 ± 0.526
3.048GluThr: 3.048 ± 0.469
5.029GluVal: 5.029 ± 0.582
1.448GluTrp: 1.448 ± 0.293
1.752GluTyr: 1.752 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
2.514PheAla: 2.514 ± 0.405
0.152PheCys: 0.152 ± 0.103
2.362PheAsp: 2.362 ± 0.431
2.286PheGlu: 2.286 ± 0.387
0.838PhePhe: 0.838 ± 0.249
3.352PheGly: 3.352 ± 0.511
0.686PheHis: 0.686 ± 0.21
1.295PheIle: 1.295 ± 0.331
1.829PheLys: 1.829 ± 0.298
2.21PheLeu: 2.21 ± 0.468
0.686PheMet: 0.686 ± 0.302
1.219PheAsn: 1.219 ± 0.271
1.143PhePro: 1.143 ± 0.324
1.067PheGln: 1.067 ± 0.304
2.362PheArg: 2.362 ± 0.39
1.905PheSer: 1.905 ± 0.276
2.743PheThr: 2.743 ± 0.459
1.905PheVal: 1.905 ± 0.323
0.61PheTrp: 0.61 ± 0.255
0.686PheTyr: 0.686 ± 0.283
0.0PheXaa: 0.0 ± 0.0
Gly
6.019GlyAla: 6.019 ± 0.925
0.686GlyCys: 0.686 ± 0.216
4.19GlyAsp: 4.19 ± 0.386
3.657GlyGlu: 3.657 ± 0.445
2.819GlyPhe: 2.819 ± 0.4
5.181GlyGly: 5.181 ± 0.868
1.829GlyHis: 1.829 ± 0.339
5.333GlyIle: 5.333 ± 1.012
5.257GlyLys: 5.257 ± 0.749
5.867GlyLeu: 5.867 ± 0.761
2.362GlyMet: 2.362 ± 0.362
2.362GlyAsn: 2.362 ± 0.411
3.276GlyPro: 3.276 ± 0.507
3.2GlyGln: 3.2 ± 0.643
4.571GlyArg: 4.571 ± 0.723
4.724GlySer: 4.724 ± 0.63
5.943GlyThr: 5.943 ± 0.787
6.248GlyVal: 6.248 ± 0.782
1.676GlyTrp: 1.676 ± 0.321
2.514GlyTyr: 2.514 ± 0.608
0.0GlyXaa: 0.0 ± 0.0
His
1.219HisAla: 1.219 ± 0.371
0.076HisCys: 0.076 ± 0.074
0.914HisAsp: 0.914 ± 0.288
1.448HisGlu: 1.448 ± 0.347
0.762HisPhe: 0.762 ± 0.249
1.981HisGly: 1.981 ± 0.369
0.381HisHis: 0.381 ± 0.163
0.762HisIle: 0.762 ± 0.299
1.143HisLys: 1.143 ± 0.342
1.295HisLeu: 1.295 ± 0.294
0.305HisMet: 0.305 ± 0.146
0.762HisAsn: 0.762 ± 0.271
0.838HisPro: 0.838 ± 0.268
0.381HisGln: 0.381 ± 0.154
0.838HisArg: 0.838 ± 0.266
1.524HisSer: 1.524 ± 0.334
1.371HisThr: 1.371 ± 0.394
1.829HisVal: 1.829 ± 0.376
0.533HisTrp: 0.533 ± 0.152
0.838HisTyr: 0.838 ± 0.189
0.0HisXaa: 0.0 ± 0.0
Ile
5.638IleAla: 5.638 ± 0.591
0.305IleCys: 0.305 ± 0.142
5.105IleAsp: 5.105 ± 0.593
3.429IleGlu: 3.429 ± 0.447
0.762IlePhe: 0.762 ± 0.316
3.962IleGly: 3.962 ± 0.749
0.99IleHis: 0.99 ± 0.289
3.124IleIle: 3.124 ± 0.735
2.514IleLys: 2.514 ± 0.526
2.819IleLeu: 2.819 ± 0.512
1.295IleMet: 1.295 ± 0.31
1.829IleAsn: 1.829 ± 0.395
2.819IlePro: 2.819 ± 0.502
2.438IleGln: 2.438 ± 0.519
2.514IleArg: 2.514 ± 0.552
3.124IleSer: 3.124 ± 0.434
3.581IleThr: 3.581 ± 0.674
3.2IleVal: 3.2 ± 0.559
0.914IleTrp: 0.914 ± 0.3
1.524IleTyr: 1.524 ± 0.297
0.0IleXaa: 0.0 ± 0.0
Lys
5.029LysAla: 5.029 ± 0.803
0.305LysCys: 0.305 ± 0.142
2.438LysAsp: 2.438 ± 0.517
3.657LysGlu: 3.657 ± 0.477
0.838LysPhe: 0.838 ± 0.212
3.352LysGly: 3.352 ± 0.519
0.99LysHis: 0.99 ± 0.267
1.905LysIle: 1.905 ± 0.412
2.362LysLys: 2.362 ± 0.553
4.419LysLeu: 4.419 ± 0.545
1.143LysMet: 1.143 ± 0.278
1.295LysAsn: 1.295 ± 0.331
3.505LysPro: 3.505 ± 0.538
2.133LysGln: 2.133 ± 0.454
2.743LysArg: 2.743 ± 0.485
2.286LysSer: 2.286 ± 0.432
2.667LysThr: 2.667 ± 0.33
3.81LysVal: 3.81 ± 0.491
1.143LysTrp: 1.143 ± 0.259
1.295LysTyr: 1.295 ± 0.249
0.0LysXaa: 0.0 ± 0.0
Leu
8.381LeuAla: 8.381 ± 0.787
0.686LeuCys: 0.686 ± 0.189
6.4LeuAsp: 6.4 ± 0.622
6.095LeuGlu: 6.095 ± 0.89
2.21LeuPhe: 2.21 ± 0.342
5.79LeuGly: 5.79 ± 0.747
1.371LeuHis: 1.371 ± 0.329
4.876LeuIle: 4.876 ± 0.951
4.648LeuLys: 4.648 ± 0.699
8.076LeuLeu: 8.076 ± 0.735
2.133LeuMet: 2.133 ± 0.414
3.2LeuAsn: 3.2 ± 0.462
3.962LeuPro: 3.962 ± 0.551
2.971LeuGln: 2.971 ± 0.462
5.714LeuArg: 5.714 ± 0.645
4.19LeuSer: 4.19 ± 0.591
5.562LeuThr: 5.562 ± 0.553
5.79LeuVal: 5.79 ± 0.631
1.143LeuTrp: 1.143 ± 0.256
1.905LeuTyr: 1.905 ± 0.373
0.0LeuXaa: 0.0 ± 0.0
Met
2.743MetAla: 2.743 ± 0.389
0.229MetCys: 0.229 ± 0.119
1.752MetAsp: 1.752 ± 0.387
1.067MetGlu: 1.067 ± 0.261
0.838MetPhe: 0.838 ± 0.228
1.752MetGly: 1.752 ± 0.382
0.229MetHis: 0.229 ± 0.145
1.219MetIle: 1.219 ± 0.264
0.381MetLys: 0.381 ± 0.172
2.667MetLeu: 2.667 ± 0.549
0.533MetMet: 0.533 ± 0.161
0.914MetAsn: 0.914 ± 0.287
1.752MetPro: 1.752 ± 0.31
0.99MetGln: 0.99 ± 0.271
0.686MetArg: 0.686 ± 0.228
3.048MetSer: 3.048 ± 0.427
2.057MetThr: 2.057 ± 0.387
1.524MetVal: 1.524 ± 0.33
0.152MetTrp: 0.152 ± 0.101
0.381MetTyr: 0.381 ± 0.168
0.0MetXaa: 0.0 ± 0.0
Asn
2.971AsnAla: 2.971 ± 0.577
0.076AsnCys: 0.076 ± 0.074
1.829AsnAsp: 1.829 ± 0.447
1.829AsnGlu: 1.829 ± 0.411
0.914AsnPhe: 0.914 ± 0.251
3.124AsnGly: 3.124 ± 0.477
0.533AsnHis: 0.533 ± 0.175
1.981AsnIle: 1.981 ± 0.421
1.524AsnLys: 1.524 ± 0.333
3.505AsnLeu: 3.505 ± 0.547
0.381AsnMet: 0.381 ± 0.148
1.143AsnAsn: 1.143 ± 0.286
1.752AsnPro: 1.752 ± 0.368
1.448AsnGln: 1.448 ± 0.327
1.905AsnArg: 1.905 ± 0.416
2.133AsnSer: 2.133 ± 0.446
1.752AsnThr: 1.752 ± 0.382
2.057AsnVal: 2.057 ± 0.374
0.686AsnTrp: 0.686 ± 0.237
1.219AsnTyr: 1.219 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
5.867ProAla: 5.867 ± 0.831
0.0ProCys: 0.0 ± 0.0
3.124ProAsp: 3.124 ± 0.449
3.276ProGlu: 3.276 ± 0.742
1.829ProPhe: 1.829 ± 0.464
4.343ProGly: 4.343 ± 0.589
0.61ProHis: 0.61 ± 0.256
2.21ProIle: 2.21 ± 0.389
1.676ProLys: 1.676 ± 0.328
3.733ProLeu: 3.733 ± 0.474
1.067ProMet: 1.067 ± 0.247
1.752ProAsn: 1.752 ± 0.354
1.371ProPro: 1.371 ± 0.37
2.59ProGln: 2.59 ± 0.511
1.981ProArg: 1.981 ± 0.462
2.59ProSer: 2.59 ± 0.367
3.505ProThr: 3.505 ± 0.632
4.876ProVal: 4.876 ± 0.674
0.61ProTrp: 0.61 ± 0.193
1.371ProTyr: 1.371 ± 0.299
0.0ProXaa: 0.0 ± 0.0
Gln
3.81GlnAla: 3.81 ± 0.585
0.152GlnCys: 0.152 ± 0.097
2.057GlnAsp: 2.057 ± 0.442
2.895GlnGlu: 2.895 ± 0.543
0.99GlnPhe: 0.99 ± 0.307
3.276GlnGly: 3.276 ± 0.54
1.448GlnHis: 1.448 ± 0.348
1.752GlnIle: 1.752 ± 0.494
1.371GlnLys: 1.371 ± 0.262
3.81GlnLeu: 3.81 ± 0.72
0.914GlnMet: 0.914 ± 0.285
1.752GlnAsn: 1.752 ± 0.423
1.524GlnPro: 1.524 ± 0.404
2.286GlnGln: 2.286 ± 0.572
2.971GlnArg: 2.971 ± 0.498
2.21GlnSer: 2.21 ± 0.433
2.667GlnThr: 2.667 ± 0.392
2.21GlnVal: 2.21 ± 0.325
1.143GlnTrp: 1.143 ± 0.268
1.067GlnTyr: 1.067 ± 0.287
0.0GlnXaa: 0.0 ± 0.0
Arg
4.571ArgAla: 4.571 ± 0.565
0.381ArgCys: 0.381 ± 0.161
3.657ArgAsp: 3.657 ± 0.431
3.352ArgGlu: 3.352 ± 0.516
2.286ArgPhe: 2.286 ± 0.415
3.352ArgGly: 3.352 ± 0.48
1.067ArgHis: 1.067 ± 0.285
3.124ArgIle: 3.124 ± 0.409
3.2ArgLys: 3.2 ± 0.549
5.41ArgLeu: 5.41 ± 0.707
2.057ArgMet: 2.057 ± 0.424
1.676ArgAsn: 1.676 ± 0.315
2.743ArgPro: 2.743 ± 0.502
2.133ArgGln: 2.133 ± 0.366
4.267ArgArg: 4.267 ± 0.739
4.343ArgSer: 4.343 ± 0.631
3.124ArgThr: 3.124 ± 0.553
4.419ArgVal: 4.419 ± 0.6
0.914ArgTrp: 0.914 ± 0.22
1.6ArgTyr: 1.6 ± 0.345
0.0ArgXaa: 0.0 ± 0.0
Ser
6.095SerAla: 6.095 ± 0.748
0.229SerCys: 0.229 ± 0.167
3.886SerAsp: 3.886 ± 0.63
2.819SerGlu: 2.819 ± 0.457
2.667SerPhe: 2.667 ± 0.46
5.029SerGly: 5.029 ± 0.729
1.067SerHis: 1.067 ± 0.287
2.819SerIle: 2.819 ± 0.62
2.743SerLys: 2.743 ± 0.465
5.257SerLeu: 5.257 ± 0.471
2.133SerMet: 2.133 ± 0.478
1.752SerAsn: 1.752 ± 0.367
2.438SerPro: 2.438 ± 0.356
2.59SerGln: 2.59 ± 0.426
3.2SerArg: 3.2 ± 0.456
3.733SerSer: 3.733 ± 0.46
3.429SerThr: 3.429 ± 0.519
4.495SerVal: 4.495 ± 0.649
1.295SerTrp: 1.295 ± 0.362
1.981SerTyr: 1.981 ± 0.352
0.0SerXaa: 0.0 ± 0.0
Thr
5.562ThrAla: 5.562 ± 1.03
0.305ThrCys: 0.305 ± 0.159
3.2ThrAsp: 3.2 ± 0.419
3.505ThrGlu: 3.505 ± 0.44
2.971ThrPhe: 2.971 ± 0.602
6.095ThrGly: 6.095 ± 0.686
1.143ThrHis: 1.143 ± 0.357
3.429ThrIle: 3.429 ± 0.472
2.286ThrLys: 2.286 ± 0.468
6.248ThrLeu: 6.248 ± 0.641
1.448ThrMet: 1.448 ± 0.344
1.067ThrAsn: 1.067 ± 0.343
3.352ThrPro: 3.352 ± 0.429
2.057ThrGln: 2.057 ± 0.349
3.505ThrArg: 3.505 ± 0.43
3.733ThrSer: 3.733 ± 0.554
4.952ThrThr: 4.952 ± 0.824
5.714ThrVal: 5.714 ± 0.706
1.295ThrTrp: 1.295 ± 0.302
2.21ThrTyr: 2.21 ± 0.447
0.0ThrXaa: 0.0 ± 0.0
Val
8.0ValAla: 8.0 ± 0.73
0.61ValCys: 0.61 ± 0.225
4.648ValAsp: 4.648 ± 0.644
4.343ValGlu: 4.343 ± 0.604
1.676ValPhe: 1.676 ± 0.31
5.714ValGly: 5.714 ± 0.789
1.448ValHis: 1.448 ± 0.327
3.733ValIle: 3.733 ± 0.59
3.886ValLys: 3.886 ± 0.576
5.562ValLeu: 5.562 ± 0.727
1.524ValMet: 1.524 ± 0.41
3.2ValAsn: 3.2 ± 0.482
3.657ValPro: 3.657 ± 0.588
2.971ValGln: 2.971 ± 0.452
4.038ValArg: 4.038 ± 0.611
4.495ValSer: 4.495 ± 0.609
5.41ValThr: 5.41 ± 0.723
5.257ValVal: 5.257 ± 0.664
1.676ValTrp: 1.676 ± 0.354
2.59ValTyr: 2.59 ± 0.473
0.0ValXaa: 0.0 ± 0.0
Trp
1.143TrpAla: 1.143 ± 0.316
0.229TrpCys: 0.229 ± 0.132
1.905TrpAsp: 1.905 ± 0.345
1.143TrpGlu: 1.143 ± 0.256
0.838TrpPhe: 0.838 ± 0.263
1.371TrpGly: 1.371 ± 0.321
0.533TrpHis: 0.533 ± 0.155
1.295TrpIle: 1.295 ± 0.286
0.686TrpLys: 0.686 ± 0.195
2.057TrpLeu: 2.057 ± 0.356
0.152TrpMet: 0.152 ± 0.115
0.762TrpAsn: 0.762 ± 0.195
0.99TrpPro: 0.99 ± 0.254
0.762TrpGln: 0.762 ± 0.278
1.143TrpArg: 1.143 ± 0.247
1.219TrpSer: 1.219 ± 0.35
1.524TrpThr: 1.524 ± 0.323
1.6TrpVal: 1.6 ± 0.375
0.61TrpTrp: 0.61 ± 0.267
0.99TrpTyr: 0.99 ± 0.235
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.895TyrAla: 2.895 ± 0.449
0.381TyrCys: 0.381 ± 0.159
2.21TyrAsp: 2.21 ± 0.372
1.981TyrGlu: 1.981 ± 0.462
0.838TyrPhe: 0.838 ± 0.233
3.352TyrGly: 3.352 ± 0.565
0.457TyrHis: 0.457 ± 0.187
1.371TyrIle: 1.371 ± 0.264
1.524TyrLys: 1.524 ± 0.333
2.133TyrLeu: 2.133 ± 0.315
0.457TyrMet: 0.457 ± 0.203
1.067TyrAsn: 1.067 ± 0.372
1.448TyrPro: 1.448 ± 0.368
0.61TyrGln: 0.61 ± 0.221
2.133TyrArg: 2.133 ± 0.428
2.133TyrSer: 2.133 ± 0.378
1.219TyrThr: 1.219 ± 0.252
2.438TyrVal: 2.438 ± 0.564
0.914TyrTrp: 0.914 ± 0.235
1.295TyrTyr: 1.295 ± 0.375
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (13126 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski