Muricauda sp.

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Muricauda; unclassified Muricauda

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2810 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M8GJ28|A0A3M8GJ28_9FLAO Dihydroxy-acid dehydratase OS=Muricauda sp. OX=192149 GN=ilvD PE=3 SV=1
MM1 pKa = 7.65AGLFFVACSNDD12 pKa = 3.53DD13 pKa = 3.8DD14 pKa = 4.4ATGPTCTDD22 pKa = 4.75GIQNGDD28 pKa = 3.59EE29 pKa = 4.23TGVDD33 pKa = 3.85CGGSCAPCEE42 pKa = 4.08VAATCDD48 pKa = 4.06DD49 pKa = 5.12GIQNGDD55 pKa = 3.9EE56 pKa = 4.21EE57 pKa = 5.49GVDD60 pKa = 4.2CGGSSCSPCTTGIEE74 pKa = 4.05IPATYY79 pKa = 10.33VFEE82 pKa = 4.99RR83 pKa = 11.84NGEE86 pKa = 4.32STVSFSGQTTRR97 pKa = 11.84LLMARR102 pKa = 11.84EE103 pKa = 3.99VLGLLRR109 pKa = 11.84DD110 pKa = 3.99NTSTEE115 pKa = 3.67ADD117 pKa = 2.77IDD119 pKa = 3.79AAFDD123 pKa = 3.74HH124 pKa = 6.68QEE126 pKa = 3.79GAMDD130 pKa = 5.78FSDD133 pKa = 5.47DD134 pKa = 5.09DD135 pKa = 5.04LNASDD140 pKa = 3.54KK141 pKa = 10.26QVRR144 pKa = 11.84NKK146 pKa = 9.38TAGSYY151 pKa = 10.04VFSQLLPAVASIAVKK166 pKa = 10.68ADD168 pKa = 3.12FDD170 pKa = 4.42GYY172 pKa = 10.36IAEE175 pKa = 4.37QVNEE179 pKa = 4.69VFPFWNQVATEE190 pKa = 4.22GNAGQLPDD198 pKa = 3.94GSSTRR203 pKa = 11.84YY204 pKa = 9.06VNARR208 pKa = 11.84GLEE211 pKa = 3.88VDD213 pKa = 3.37QAFIKK218 pKa = 10.69GLTGAFVSDD227 pKa = 4.01QILNNYY233 pKa = 7.43TDD235 pKa = 4.5PGQLAQFEE243 pKa = 4.82DD244 pKa = 4.96DD245 pKa = 4.01NDD247 pKa = 3.62NDD249 pKa = 4.04VIVDD253 pKa = 4.23GKK255 pKa = 9.5TYY257 pKa = 10.88TDD259 pKa = 4.54MEE261 pKa = 4.9HH262 pKa = 7.29DD263 pKa = 3.17WDD265 pKa = 3.56EE266 pKa = 4.37AYY268 pKa = 10.52GYY270 pKa = 10.28FFGVAADD277 pKa = 4.04GEE279 pKa = 4.66NPVAALLDD287 pKa = 4.23GGDD290 pKa = 4.04PGDD293 pKa = 3.97FSSGSDD299 pKa = 3.18EE300 pKa = 4.48FLYY303 pKa = 10.56KK304 pKa = 10.79YY305 pKa = 10.55VDD307 pKa = 3.43NVEE310 pKa = 4.46ANFDD314 pKa = 3.62GTAAAIFDD322 pKa = 3.97AYY324 pKa = 10.02KK325 pKa = 10.52AGRR328 pKa = 11.84TAIVNKK334 pKa = 10.57DD335 pKa = 3.07YY336 pKa = 11.36DD337 pKa = 3.93EE338 pKa = 5.84RR339 pKa = 11.84DD340 pKa = 3.44AQADD344 pKa = 3.4ILRR347 pKa = 11.84EE348 pKa = 4.12KK349 pKa = 10.41IAMLVAVRR357 pKa = 11.84AIHH360 pKa = 5.46YY361 pKa = 8.28LKK363 pKa = 10.82AGASGVGGTNQGGTFHH379 pKa = 7.57DD380 pKa = 4.11LSEE383 pKa = 4.98AYY385 pKa = 10.21GFIYY389 pKa = 10.38SLQFLRR395 pKa = 11.84SPGSDD400 pKa = 2.63LAFYY404 pKa = 9.59FSRR407 pKa = 11.84DD408 pKa = 3.28EE409 pKa = 3.92VLGYY413 pKa = 10.77LDD415 pKa = 4.65TIYY418 pKa = 9.52NTPTNGFWNVTTEE431 pKa = 4.02NLDD434 pKa = 3.6EE435 pKa = 4.2VAEE438 pKa = 4.69AIAAKK443 pKa = 10.48FSFTAAEE450 pKa = 3.87VDD452 pKa = 3.42

Molecular weight: 48.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M8GQ66|A0A3M8GQ66_9FLAO Uncharacterized protein OS=Muricauda sp. OX=192149 GN=ED555_04480 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84MFYY5 pKa = 10.79GILYY9 pKa = 7.91YY10 pKa = 9.77FQKK13 pKa = 10.55RR14 pKa = 11.84GFEE17 pKa = 3.99VCRR20 pKa = 11.84RR21 pKa = 11.84VAEE24 pKa = 4.14RR25 pKa = 11.84FGIRR29 pKa = 11.84ARR31 pKa = 11.84VVRR34 pKa = 11.84TTFIYY39 pKa = 9.47LTFATLGFGFALYY52 pKa = 10.87LFMAFWLRR60 pKa = 11.84IKK62 pKa = 10.7DD63 pKa = 4.08LIYY66 pKa = 10.52TKK68 pKa = 9.75RR69 pKa = 11.84TSVFDD74 pKa = 3.66LL75 pKa = 4.18

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2810

0

2810

964233

38

4548

343.1

38.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.125 ± 0.044

0.72 ± 0.016

5.763 ± 0.045

6.626 ± 0.051

5.248 ± 0.039

7.026 ± 0.05

1.887 ± 0.027

7.019 ± 0.037

6.861 ± 0.076

9.638 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.026

5.5 ± 0.042

3.613 ± 0.03

3.65 ± 0.024

3.894 ± 0.035

6.104 ± 0.036

5.576 ± 0.065

6.535 ± 0.033

1.144 ± 0.019

3.793 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski