Port-miou virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Marseilleviridae; Lausannevirus

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 410 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9PVH4|A0A0N9PVH4_9VIRU Uncharacterized protein OS=Port-miou virus OX=1733873 GN=PMV_149 PE=4 SV=1
MM1 pKa = 7.49SRR3 pKa = 11.84LQGFFKK9 pKa = 10.51DD10 pKa = 3.47IPIVGSYY17 pKa = 10.37SALPPVLPRR26 pKa = 11.84GSLVFLTSDD35 pKa = 3.11EE36 pKa = 4.21TLYY39 pKa = 11.38VSDD42 pKa = 3.59GTTWNATGGDD52 pKa = 3.95IGPLAAQVAQNTADD66 pKa = 3.22IGVLQTQVGTLQGEE80 pKa = 4.73VATNTLDD87 pKa = 3.32IGTLQTQVSTNTSDD101 pKa = 3.09IGTLQTQVAGNSTNISALQTDD122 pKa = 4.12VATLEE127 pKa = 4.4GQVATNTTDD136 pKa = 2.67IGTLQSQVATNTTNIQQNSDD156 pKa = 4.12SITALLINQSQGFSILDD173 pKa = 3.48NTQTRR178 pKa = 11.84AYY180 pKa = 6.68TQPISRR186 pKa = 11.84VDD188 pKa = 3.54VASVSPTVIYY198 pKa = 10.0TLGIPLAININAVYY212 pKa = 10.13LIQWNVLAHH221 pKa = 6.05YY222 pKa = 7.5EE223 pKa = 4.06TANVYY228 pKa = 9.77FAVGQSSVLSNGVGSLVSVFGTTTNQTNNSGAANIAVTADD268 pKa = 3.8FSTPNFRR275 pKa = 11.84LIVTSDD281 pKa = 3.24SAVNTTYY288 pKa = 10.59SGYY291 pKa = 11.11VIVTNILNIVPP302 pKa = 4.08

Molecular weight: 31.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9PZE7|A0A0N9PZE7_9VIRU Uncharacterized protein OS=Port-miou virus OX=1733873 GN=PMV_395 PE=4 SV=1
MM1 pKa = 6.64QQRR4 pKa = 11.84LVNPEE9 pKa = 3.48TGRR12 pKa = 11.84NVLYY16 pKa = 10.64GGKK19 pKa = 7.33THH21 pKa = 7.11LNLVRR26 pKa = 11.84RR27 pKa = 11.84GILPRR32 pKa = 11.84EE33 pKa = 4.01EE34 pKa = 4.1PVKK37 pKa = 10.77KK38 pKa = 10.43GAGGSNVRR46 pKa = 11.84KK47 pKa = 9.73YY48 pKa = 10.73KK49 pKa = 10.37KK50 pKa = 9.1EE51 pKa = 3.7HH52 pKa = 6.04LKK54 pKa = 10.93ASEE57 pKa = 4.21FCGTVPGSFPVNTEE71 pKa = 3.4RR72 pKa = 11.84RR73 pKa = 11.84AKK75 pKa = 10.48AALSYY80 pKa = 10.95ARR82 pKa = 11.84NDD84 pKa = 3.41VNPEE88 pKa = 3.76RR89 pKa = 11.84VRR91 pKa = 11.84EE92 pKa = 4.01CARR95 pKa = 11.84RR96 pKa = 11.84KK97 pKa = 8.83AKK99 pKa = 10.52RR100 pKa = 11.84MGWFF104 pKa = 3.21

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

410

0

410

103435

50

1525

252.3

28.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.266 ± 0.114

2.457 ± 0.109

4.926 ± 0.071

8.213 ± 0.164

5.539 ± 0.09

6.2 ± 0.134

1.78 ± 0.057

5.084 ± 0.079

8.709 ± 0.2

8.729 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.037 ± 0.05

4.132 ± 0.095

3.932 ± 0.106

3.482 ± 0.09

5.4 ± 0.099

7.51 ± 0.151

5.359 ± 0.125

6.525 ± 0.078

1.505 ± 0.048

3.216 ± 0.065
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski