Amino acid dipepetide frequency for Bifidobacterium longum (strain NCC 2705)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	14.051AlaAla: 14.051 ± 0.257	1.122AlaCys: 1.122 ± 0.044	7.77AlaAsp: 7.77 ± 0.14	6.4AlaGlu: 6.4 ± 0.12	3.364AlaPhe: 3.364 ± 0.076	9.235AlaGly: 9.235 ± 0.144	2.226AlaHis: 2.226 ± 0.064	5.757AlaIle: 5.757 ± 0.108	5.112AlaLys: 5.112 ± 0.096	9.775AlaLeu: 9.775 ± 0.15	3.113AlaMet: 3.113 ± 0.077	3.655AlaAsn: 3.655 ± 0.073	4.218AlaPro: 4.218 ± 0.11	4.242AlaGln: 4.242 ± 0.101	5.805AlaArg: 5.805 ± 0.121	6.63AlaSer: 6.63 ± 0.119	6.363AlaThr: 6.363 ± 0.116	8.623AlaVal: 8.623 ± 0.132	1.377AlaTrp: 1.377 ± 0.049	2.684AlaTyr: 2.684 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	1.066CysAla: 1.066 ± 0.047	0.139CysCys: 0.139 ± 0.017	0.527CysAsp: 0.527 ± 0.029	0.484CysGlu: 0.484 ± 0.033	0.316CysPhe: 0.316 ± 0.022	1.002CysGly: 1.002 ± 0.042	0.236CysHis: 0.236 ± 0.023	0.394CysIle: 0.394 ± 0.028	0.301CysLys: 0.301 ± 0.021	0.751CysLeu: 0.751 ± 0.039	0.236CysMet: 0.236 ± 0.021	0.25CysAsn: 0.25 ± 0.021	0.481CysPro: 0.481 ± 0.029	0.235CysGln: 0.235 ± 0.021	0.635CysArg: 0.635 ± 0.032	0.626CysSer: 0.626 ± 0.03	0.538CysThr: 0.538 ± 0.028	0.795CysVal: 0.795 ± 0.037	0.15CysTrp: 0.15 ± 0.016	0.216CysTyr: 0.216 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	7.017AspAla: 7.017 ± 0.126	0.532AspCys: 0.532 ± 0.027	4.539AspAsp: 4.539 ± 0.122	4.735AspGlu: 4.735 ± 0.101	2.32AspPhe: 2.32 ± 0.057	5.848AspGly: 5.848 ± 0.117	1.315AspHis: 1.315 ± 0.051	3.404AspIle: 3.404 ± 0.078	2.6AspLys: 2.6 ± 0.082	5.29AspLeu: 5.29 ± 0.088	1.703AspMet: 1.703 ± 0.052	1.983AspAsn: 1.983 ± 0.067	3.436AspPro: 3.436 ± 0.081	2.069AspGln: 2.069 ± 0.056	3.223AspArg: 3.223 ± 0.092	3.728AspSer: 3.728 ± 0.099	3.489AspThr: 3.489 ± 0.093	4.968AspVal: 4.968 ± 0.097	1.039AspTrp: 1.039 ± 0.042	1.955AspTyr: 1.955 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	6.324GluAla: 6.324 ± 0.108	0.451GluCys: 0.451 ± 0.024	3.231GluAsp: 3.231 ± 0.085	3.243GluGlu: 3.243 ± 0.092	1.849GluPhe: 1.849 ± 0.057	3.91GluGly: 3.91 ± 0.088	1.747GluHis: 1.747 ± 0.055	2.777GluIle: 2.777 ± 0.066	2.155GluLys: 2.155 ± 0.069	5.228GluLeu: 5.228 ± 0.093	1.359GluMet: 1.359 ± 0.052	1.999GluAsn: 1.999 ± 0.056	2.708GluPro: 2.708 ± 0.07	2.684GluGln: 2.684 ± 0.068	3.902GluArg: 3.902 ± 0.09	3.483GluSer: 3.483 ± 0.07	3.45GluThr: 3.45 ± 0.079	3.713GluVal: 3.713 ± 0.079	0.664GluTrp: 0.664 ± 0.034	1.741GluTyr: 1.741 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	3.991PheAla: 3.991 ± 0.092	0.377PheCys: 0.377 ± 0.023	2.705PheAsp: 2.705 ± 0.064	1.838PheGlu: 1.838 ± 0.061	1.213PhePhe: 1.213 ± 0.056	3.253PheGly: 3.253 ± 0.072	0.747PheHis: 0.747 ± 0.035	1.737PheIle: 1.737 ± 0.058	1.177PheLys: 1.177 ± 0.047	2.625PheLeu: 2.625 ± 0.078	0.783PheMet: 0.783 ± 0.038	1.219PheAsn: 1.219 ± 0.048	1.387PhePro: 1.387 ± 0.053	0.97PheGln: 0.97 ± 0.038	1.603PheArg: 1.603 ± 0.056	2.168PheSer: 2.168 ± 0.06	2.365PheThr: 2.365 ± 0.068	2.639PheVal: 2.639 ± 0.072	0.47PheTrp: 0.47 ± 0.029	0.88PheTyr: 0.88 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	7.554GlyAla: 7.554 ± 0.117	0.795GlyCys: 0.795 ± 0.036	4.724GlyAsp: 4.724 ± 0.099	4.65GlyGlu: 4.65 ± 0.096	3.21GlyPhe: 3.21 ± 0.076	6.295GlyGly: 6.295 ± 0.134	1.85GlyHis: 1.85 ± 0.055	4.766GlyIle: 4.766 ± 0.103	4.023GlyLys: 4.023 ± 0.082	7.181GlyLeu: 7.181 ± 0.136	2.126GlyMet: 2.126 ± 0.062	2.788GlyAsn: 2.788 ± 0.09	2.705GlyPro: 2.705 ± 0.066	2.755GlyGln: 2.755 ± 0.096	4.422GlyArg: 4.422 ± 0.108	5.269GlySer: 5.269 ± 0.116	5.254GlyThr: 5.254 ± 0.11	6.267GlyVal: 6.267 ± 0.097	1.243GlyTrp: 1.243 ± 0.048	2.579GlyTyr: 2.579 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	2.223HisAla: 2.223 ± 0.063	0.266HisCys: 0.266 ± 0.018	1.528HisAsp: 1.528 ± 0.057	1.268HisGlu: 1.268 ± 0.05	0.646HisPhe: 0.646 ± 0.031	2.003HisGly: 2.003 ± 0.056	0.617HisHis: 0.617 ± 0.031	1.254HisIle: 1.254 ± 0.045	0.67HisLys: 0.67 ± 0.037	1.665HisLeu: 1.665 ± 0.054	0.579HisMet: 0.579 ± 0.035	0.667HisAsn: 0.667 ± 0.035	1.298HisPro: 1.298 ± 0.05	0.667HisGln: 0.667 ± 0.034	1.498HisArg: 1.498 ± 0.059	1.063HisSer: 1.063 ± 0.045	1.247HisThr: 1.247 ± 0.044	1.861HisVal: 1.861 ± 0.054	0.313HisTrp: 0.313 ± 0.019	0.554HisTyr: 0.554 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	6.846IleAla: 6.846 ± 0.109	0.618IleCys: 0.618 ± 0.037	4.041IleAsp: 4.041 ± 0.091	3.262IleGlu: 3.262 ± 0.084	1.493IlePhe: 1.493 ± 0.052	4.667IleGly: 4.667 ± 0.102	1.019IleHis: 1.019 ± 0.042	2.914IleIle: 2.914 ± 0.079	1.855IleLys: 1.855 ± 0.058	3.979IleLeu: 3.979 ± 0.092	1.18IleMet: 1.18 ± 0.046	1.892IleAsn: 1.892 ± 0.056	2.694IlePro: 2.694 ± 0.066	1.399IleGln: 1.399 ± 0.043	2.903IleArg: 2.903 ± 0.08	3.11IleSer: 3.11 ± 0.086	3.5IleThr: 3.5 ± 0.061	4.686IleVal: 4.686 ± 0.109	0.576IleTrp: 0.576 ± 0.029	1.103IleTyr: 1.103 ± 0.044	0.0IleXaa: 0.0 ± 0.0
Lys	5.139LysAla: 5.139 ± 0.114	0.203LysCys: 0.203 ± 0.018	2.619LysAsp: 2.619 ± 0.082	2.135LysGlu: 2.135 ± 0.066	1.072LysPhe: 1.072 ± 0.041	2.705LysGly: 2.705 ± 0.07	0.848LysHis: 0.848 ± 0.037	1.894LysIle: 1.894 ± 0.061	1.933LysLys: 1.933 ± 0.062	3.475LysLeu: 3.475 ± 0.086	0.953LysMet: 0.953 ± 0.037	1.512LysAsn: 1.512 ± 0.052	2.428LysPro: 2.428 ± 0.062	1.543LysGln: 1.543 ± 0.053	2.457LysArg: 2.457 ± 0.072	2.478LysSer: 2.478 ± 0.077	2.656LysThr: 2.656 ± 0.066	2.957LysVal: 2.957 ± 0.104	0.501LysTrp: 0.501 ± 0.031	1.124LysTyr: 1.124 ± 0.045	0.0LysXaa: 0.0 ± 0.0
Leu	10.179LeuAla: 10.179 ± 0.145	0.9LeuCys: 0.9 ± 0.043	6.026LeuAsp: 6.026 ± 0.103	4.525LeuGlu: 4.525 ± 0.099	3.082LeuPhe: 3.082 ± 0.081	6.99LeuGly: 6.99 ± 0.114	1.797LeuHis: 1.797 ± 0.06	4.796LeuIle: 4.796 ± 0.116	3.877LeuLys: 3.877 ± 0.086	7.474LeuLeu: 7.474 ± 0.145	2.147LeuMet: 2.147 ± 0.062	3.021LeuAsn: 3.021 ± 0.065	4.481LeuPro: 4.481 ± 0.088	2.312LeuGln: 2.312 ± 0.062	4.758LeuArg: 4.758 ± 0.09	5.316LeuSer: 5.316 ± 0.114	5.735LeuThr: 5.735 ± 0.099	6.416LeuVal: 6.416 ± 0.128	0.978LeuTrp: 0.978 ± 0.045	1.858LeuTyr: 1.858 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.874MetAla: 2.874 ± 0.074	0.263MetCys: 0.263 ± 0.019	1.357MetAsp: 1.357 ± 0.046	1.105MetGlu: 1.105 ± 0.043	0.853MetPhe: 0.853 ± 0.039	1.855MetGly: 1.855 ± 0.065	0.517MetHis: 0.517 ± 0.028	1.262MetIle: 1.262 ± 0.05	0.963MetLys: 0.963 ± 0.04	2.495MetLeu: 2.495 ± 0.071	0.715MetMet: 0.715 ± 0.037	0.923MetAsn: 0.923 ± 0.045	1.528MetPro: 1.528 ± 0.057	0.803MetGln: 0.803 ± 0.041	1.618MetArg: 1.618 ± 0.052	1.78MetSer: 1.78 ± 0.058	2.032MetThr: 2.032 ± 0.058	1.839MetVal: 1.839 ± 0.05	0.33MetTrp: 0.33 ± 0.022	0.518MetTyr: 0.518 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	4.026AsnAla: 4.026 ± 0.09	0.216AsnCys: 0.216 ± 0.021	2.06AsnAsp: 2.06 ± 0.069	1.859AsnGlu: 1.859 ± 0.063	0.986AsnPhe: 0.986 ± 0.044	3.249AsnGly: 3.249 ± 0.091	0.711AsnHis: 0.711 ± 0.033	1.714AsnIle: 1.714 ± 0.047	1.359AsnLys: 1.359 ± 0.057	2.869AsnLeu: 2.869 ± 0.075	0.79AsnMet: 0.79 ± 0.034	1.24AsnAsn: 1.24 ± 0.061	2.299AsnPro: 2.299 ± 0.055	1.136AsnGln: 1.136 ± 0.049	2.039AsnArg: 2.039 ± 0.065	1.736AsnSer: 1.736 ± 0.056	2.229AsnThr: 2.229 ± 0.076	2.468AsnVal: 2.468 ± 0.058	0.474AsnTrp: 0.474 ± 0.032	0.886AsnTyr: 0.886 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	5.463ProAla: 5.463 ± 0.116	0.36ProCys: 0.36 ± 0.026	3.395ProAsp: 3.395 ± 0.075	3.368ProGlu: 3.368 ± 0.079	1.706ProPhe: 1.706 ± 0.059	3.544ProGly: 3.544 ± 0.09	0.977ProHis: 0.977 ± 0.044	2.338ProIle: 2.338 ± 0.063	1.833ProLys: 1.833 ± 0.06	3.753ProLeu: 3.753 ± 0.087	1.103ProMet: 1.103 ± 0.04	1.69ProAsn: 1.69 ± 0.05	1.337ProPro: 1.337 ± 0.057	1.813ProGln: 1.813 ± 0.068	2.362ProArg: 2.362 ± 0.062	3.049ProSer: 3.049 ± 0.082	2.891ProThr: 2.891 ± 0.076	4.002ProVal: 4.002 ± 0.082	0.689ProTrp: 0.689 ± 0.038	1.426ProTyr: 1.426 ± 0.05	0.0ProXaa: 0.0 ± 0.0
Gln	3.821GlnAla: 3.821 ± 0.081	0.301GlnCys: 0.301 ± 0.024	1.515GlnAsp: 1.515 ± 0.051	1.47GlnGlu: 1.47 ± 0.047	1.149GlnPhe: 1.149 ± 0.042	2.354GlnGly: 2.354 ± 0.08	0.831GlnHis: 0.831 ± 0.042	1.983GlnIle: 1.983 ± 0.048	1.119GlnLys: 1.119 ± 0.042	3.298GlnLeu: 3.298 ± 0.074	0.95GlnMet: 0.95 ± 0.039	1.057GlnAsn: 1.057 ± 0.047	1.886GlnPro: 1.886 ± 0.093	1.669GlnGln: 1.669 ± 0.07	2.367GlnArg: 2.367 ± 0.07	2.23GlnSer: 2.23 ± 0.072	1.993GlnThr: 1.993 ± 0.062	2.478GlnVal: 2.478 ± 0.07	0.543GlnTrp: 0.543 ± 0.03	1.105GlnTyr: 1.105 ± 0.046	0.0GlnXaa: 0.0 ± 0.0
Arg	5.222ArgAla: 5.222 ± 0.104	0.526ArgCys: 0.526 ± 0.033	3.345ArgAsp: 3.345 ± 0.087	3.67ArgGlu: 3.67 ± 0.086	2.205ArgPhe: 2.205 ± 0.061	3.703ArgGly: 3.703 ± 0.086	1.496ArgHis: 1.496 ± 0.05	3.429ArgIle: 3.429 ± 0.078	2.562ArgLys: 2.562 ± 0.072	5.237ArgLeu: 5.237 ± 0.105	1.739ArgMet: 1.739 ± 0.054	2.008ArgAsn: 2.008 ± 0.054	2.507ArgPro: 2.507 ± 0.066	2.088ArgGln: 2.088 ± 0.06	4.528ArgArg: 4.528 ± 0.142	3.112ArgSer: 3.112 ± 0.075	3.328ArgThr: 3.328 ± 0.077	4.048ArgVal: 4.048 ± 0.087	0.826ArgTrp: 0.826 ± 0.041	1.772ArgTyr: 1.772 ± 0.061	0.0ArgXaa: 0.0 ± 0.0
Ser	6.579SerAla: 6.579 ± 0.121	0.518SerCys: 0.518 ± 0.033	4.021SerAsp: 4.021 ± 0.097	3.029SerGlu: 3.029 ± 0.073	2.071SerPhe: 2.071 ± 0.061	5.614SerGly: 5.614 ± 0.121	1.318SerHis: 1.318 ± 0.05	3.265SerIle: 3.265 ± 0.082	2.332SerLys: 2.332 ± 0.062	5.308SerLeu: 5.308 ± 0.105	1.678SerMet: 1.678 ± 0.06	2.154SerAsn: 2.154 ± 0.071	2.598SerPro: 2.598 ± 0.071	2.177SerGln: 2.177 ± 0.054	3.451SerArg: 3.451 ± 0.097	4.271SerSer: 4.271 ± 0.117	3.827SerThr: 3.827 ± 0.083	4.519SerVal: 4.519 ± 0.096	0.917SerTrp: 0.917 ± 0.042	1.631SerTyr: 1.631 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	6.64ThrAla: 6.64 ± 0.132	0.523ThrCys: 0.523 ± 0.025	3.974ThrAsp: 3.974 ± 0.091	3.038ThrGlu: 3.038 ± 0.071	2.157ThrPhe: 2.157 ± 0.069	5.442ThrGly: 5.442 ± 0.111	1.277ThrHis: 1.277 ± 0.049	3.667ThrIle: 3.667 ± 0.082	2.24ThrLys: 2.24 ± 0.064	5.724ThrLeu: 5.724 ± 0.092	1.495ThrMet: 1.495 ± 0.045	2.038ThrAsn: 2.038 ± 0.07	3.476ThrPro: 3.476 ± 0.079	1.947ThrGln: 1.947 ± 0.062	3.055ThrArg: 3.055 ± 0.075	3.638ThrSer: 3.638 ± 0.092	3.965ThrThr: 3.965 ± 0.095	5.722ThrVal: 5.722 ± 0.139	0.773ThrTrp: 0.773 ± 0.038	1.741ThrTyr: 1.741 ± 0.068	0.0ThrXaa: 0.0 ± 0.0
Val	8.357ValAla: 8.357 ± 0.12	0.803ValCys: 0.803 ± 0.039	5.153ValAsp: 5.153 ± 0.103	4.376ValGlu: 4.376 ± 0.088	2.932ValPhe: 2.932 ± 0.073	5.3ValGly: 5.3 ± 0.105	1.493ValHis: 1.493 ± 0.051	4.458ValIle: 4.458 ± 0.091	3.077ValLys: 3.077 ± 0.072	6.934ValLeu: 6.934 ± 0.133	2.016ValMet: 2.016 ± 0.064	2.722ValAsn: 2.722 ± 0.068	3.825ValPro: 3.825 ± 0.082	2.046ValGln: 2.046 ± 0.056	4.181ValArg: 4.181 ± 0.083	5.132ValSer: 5.132 ± 0.089	5.29ValThr: 5.29 ± 0.126	6.233ValVal: 6.233 ± 0.125	0.949ValTrp: 0.949 ± 0.041	1.802ValTyr: 1.802 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	1.179TrpAla: 1.179 ± 0.054	0.161TrpCys: 0.161 ± 0.018	0.787TrpAsp: 0.787 ± 0.036	0.587TrpGlu: 0.587 ± 0.034	0.574TrpPhe: 0.574 ± 0.034	0.894TrpGly: 0.894 ± 0.039	0.349TrpHis: 0.349 ± 0.022	0.679TrpIle: 0.679 ± 0.036	0.603TrpLys: 0.603 ± 0.033	1.39TrpLeu: 1.39 ± 0.055	0.385TrpMet: 0.385 ± 0.025	0.629TrpAsn: 0.629 ± 0.034	0.576TrpPro: 0.576 ± 0.033	0.618TrpGln: 0.618 ± 0.033	0.908TrpArg: 0.908 ± 0.043	0.825TrpSer: 0.825 ± 0.036	0.831TrpThr: 0.831 ± 0.039	0.883TrpVal: 0.883 ± 0.038	0.258TrpTrp: 0.258 ± 0.021	0.421TrpTyr: 0.421 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.975TyrAla: 2.975 ± 0.062	0.305TyrCys: 0.305 ± 0.023	1.877TyrAsp: 1.877 ± 0.069	1.571TyrGlu: 1.571 ± 0.054	0.947TyrPhe: 0.947 ± 0.04	2.592TyrGly: 2.592 ± 0.067	0.517TyrHis: 0.517 ± 0.029	1.251TyrIle: 1.251 ± 0.05	0.842TyrLys: 0.842 ± 0.043	2.216TyrLeu: 2.216 ± 0.062	0.576TyrMet: 0.576 ± 0.033	0.9TyrAsn: 0.9 ± 0.037	1.232TyrPro: 1.232 ± 0.048	0.944TyrGln: 0.944 ± 0.038	1.636TyrArg: 1.636 ± 0.052	1.636TyrSer: 1.636 ± 0.054	1.55TyrThr: 1.55 ± 0.064	1.963TyrVal: 1.963 ± 0.053	0.466TyrTrp: 0.466 ± 0.031	0.795TyrTyr: 0.795 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1725 proteins (638887 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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