Sulfobacillus acidophilus (strain ATCC 700253 / DSM 10332 / NAL)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Clostridiales Family XVII. Incertae Sedis; Sulfobacillus; Sulfobacillus acidophilus

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3444 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8U176|G8U176_SULAD Uncharacterized protein OS=Sulfobacillus acidophilus (strain ATCC 700253 / DSM 10332 / NAL) OX=679936 GN=Sulac_0806 PE=4 SV=1
MM1 pKa = 7.6AFDD4 pKa = 4.16ILNIASRR11 pKa = 11.84AIAAQQLALEE21 pKa = 4.37VTGNNMANATTPGYY35 pKa = 9.72HH36 pKa = 6.06VEE38 pKa = 4.22SAVLAEE44 pKa = 4.88AMPSPDD50 pKa = 3.27PTEE53 pKa = 4.2PNALVGQGVDD63 pKa = 3.13VLTVSRR69 pKa = 11.84AANAFLSRR77 pKa = 11.84SVRR80 pKa = 11.84TQQSILGYY88 pKa = 10.21DD89 pKa = 3.33QAKK92 pKa = 9.68SQGLGEE98 pKa = 4.2VQNIFQEE105 pKa = 4.31PAPQGLAEE113 pKa = 4.16TMNAFFQSWLTLSEE127 pKa = 4.29NPTSVAAEE135 pKa = 3.98EE136 pKa = 5.14GVIQQGQNLSTVFNQMANTLTSQIASVNQNIGNLVTQVNQLATQIAQINQQIVTVGSSQEE196 pKa = 3.97PPNNLMDD203 pKa = 3.7QRR205 pKa = 11.84SALVDD210 pKa = 3.48QLSKK214 pKa = 10.96LVNISYY220 pKa = 10.99APGPDD225 pKa = 3.12NTMDD229 pKa = 3.62IYY231 pKa = 10.63IGTHH235 pKa = 5.92PLVSGNQASTLVTTANALGFNQPSWADD262 pKa = 3.22SGAPAAITSGTLGGNMALLYY282 pKa = 9.65TSSGSSGSPPGNGYY296 pKa = 8.22LTGYY300 pKa = 9.31LQSLNNLASAIVTQVNAQFNAGFTTSGTAPSQPFFVTTGTTAATIAVTPNLPPSDD355 pKa = 3.31IAAASSPNSPGDD367 pKa = 3.43GSNASAIFNLSQTAEE382 pKa = 4.63PIGSLTTTYY391 pKa = 9.85GQYY394 pKa = 8.94YY395 pKa = 5.92TTLVGQVGMDD405 pKa = 3.46GQSAEE410 pKa = 4.02NAANQDD416 pKa = 3.5QTTLTSLSNALQSATGVDD434 pKa = 3.55INQQSVQMIQEE445 pKa = 4.12QQSYY449 pKa = 7.7MAAAKK454 pKa = 9.92LVQTQQSIIQSLLAAVSS471 pKa = 3.45

Molecular weight: 48.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8TV16|G8TV16_SULAD Uncharacterized protein OS=Sulfobacillus acidophilus (strain ATCC 700253 / DSM 10332 / NAL) OX=679936 GN=Sulac_0020 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNRR9 pKa = 11.84RR10 pKa = 11.84HH11 pKa = 5.61RR12 pKa = 11.84AKK14 pKa = 10.59VHH16 pKa = 5.69GFRR19 pKa = 11.84QRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84QGRR28 pKa = 11.84AVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.22GRR39 pKa = 11.84KK40 pKa = 8.7RR41 pKa = 11.84LAGG44 pKa = 3.61

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3444

0

3444

1017646

30

1580

295.5

32.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.302 ± 0.044

0.553 ± 0.012

4.891 ± 0.031

5.347 ± 0.044

3.372 ± 0.028

8.19 ± 0.038

2.629 ± 0.019

5.23 ± 0.029

2.408 ± 0.025

10.78 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.35 ± 0.019

2.364 ± 0.025

5.951 ± 0.033

4.13 ± 0.027

7.253 ± 0.043

5.106 ± 0.029

5.744 ± 0.03

8.346 ± 0.033

2.363 ± 0.026

2.693 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski