Algibacter pectinivorans
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3126 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I1Q546|A0A1I1Q546_9FLAO TPM_phosphatase domain-containing protein OS=Algibacter pectinivorans OX=870482 GN=SAMN04487987_104232 PE=4 SV=1
MM1 pKa = 7.02 KK2 pKa = 10.0 TSLLFVVCIVMTIMSCNDD20 pKa = 3.06 EE21 pKa = 5.3 DD22 pKa = 4.74 DD23 pKa = 4.05 SQGINSTPEE32 pKa = 3.98 FISFVDD38 pKa = 5.39 AISSLPGEE46 pKa = 4.37 TLTFEE51 pKa = 5.33 AGISDD56 pKa = 3.82 PAGIKK61 pKa = 10.11 SINIKK66 pKa = 10.02 YY67 pKa = 9.83 EE68 pKa = 3.67 SWFLDD73 pKa = 2.96 KK74 pKa = 10.96 TIFKK78 pKa = 10.66 DD79 pKa = 3.52 SLPDD83 pKa = 3.67 VYY85 pKa = 11.0 EE86 pKa = 3.77 LAYY89 pKa = 10.79 DD90 pKa = 4.6 FIVPDD95 pKa = 4.2 DD96 pKa = 4.32 AEE98 pKa = 4.44 MNSVHH103 pKa = 6.51 TIVITAYY110 pKa = 10.51 NVGGNQITKK119 pKa = 10.25 NVVVTLDD126 pKa = 3.62 KK127 pKa = 11.27 DD128 pKa = 3.88 VTNPTIQIVSPTNQSTVLIGDD149 pKa = 3.6 GNEE152 pKa = 3.59 IVLDD156 pKa = 3.62 ITVSDD161 pKa = 3.97 AEE163 pKa = 4.03 LAEE166 pKa = 4.32 FKK168 pKa = 10.59 IEE170 pKa = 4.13 SSVLNEE176 pKa = 3.77 TKK178 pKa = 10.42 AISGEE183 pKa = 4.11 TYY185 pKa = 10.51 NYY187 pKa = 9.63 IRR189 pKa = 11.84 ALDD192 pKa = 3.52 ISTLGNYY199 pKa = 8.17 EE200 pKa = 3.86 FAITVTDD207 pKa = 3.5 ASGNIRR213 pKa = 11.84 TEE215 pKa = 4.28 TISVNVLDD223 pKa = 4.24 EE224 pKa = 4.41 LLFEE228 pKa = 4.41 VMYY231 pKa = 9.5 ITDD234 pKa = 3.68 VTSNAALNDD243 pKa = 3.82 DD244 pKa = 4.57 LFGVPYY250 pKa = 9.62 STIASTASTEE260 pKa = 3.95 DD261 pKa = 4.03 GYY263 pKa = 11.96 VFTGKK268 pKa = 10.48 YY269 pKa = 9.9 YY270 pKa = 10.83 SSTPNSEE277 pKa = 3.65 VRR279 pKa = 11.84 FIPQQTAFEE288 pKa = 4.84 PYY290 pKa = 10.6 SFGADD295 pKa = 3.33 PNAMGEE301 pKa = 4.38 LILGNDD307 pKa = 3.61 ASVSPIVVPGVGYY320 pKa = 9.9 YY321 pKa = 10.11 EE322 pKa = 3.81 ITMDD326 pKa = 5.79 LRR328 pKa = 11.84 DD329 pKa = 3.23 QSYY332 pKa = 8.99 VVEE335 pKa = 4.74 PYY337 pKa = 11.06 VPIDD341 pKa = 3.27 TAFDD345 pKa = 3.4 QVYY348 pKa = 8.89 VLGRR352 pKa = 11.84 GVYY355 pKa = 10.39 LDD357 pKa = 4.07 ATSSTCVDD365 pKa = 3.34 NSDD368 pKa = 2.95 GSTRR372 pKa = 11.84 CWHH375 pKa = 6.17 FLSGKK380 pKa = 9.53 PFIPDD385 pKa = 3.19 SNNPYY390 pKa = 10.43 LWTIDD395 pKa = 3.2 VTAQDD400 pKa = 3.67 QPDD403 pKa = 3.78 DD404 pKa = 3.7 NGANGFILNANPEE417 pKa = 3.86 GWGPFWRR424 pKa = 11.84 VDD426 pKa = 3.48 ANDD429 pKa = 3.97 PSVTIPNAGANYY441 pKa = 9.94 VFDD444 pKa = 5.61 DD445 pKa = 3.62 SSLNKK450 pKa = 10.46 DD451 pKa = 3.26 YY452 pKa = 11.0 TFVFDD457 pKa = 3.24 THH459 pKa = 6.93 LNRR462 pKa = 11.84 LVVKK466 pKa = 10.66 NRR468 pKa = 3.29
Molecular weight: 51.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.703
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.935
Patrickios 0.935
IPC_peptide 3.808
IPC2_peptide 3.91
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A1I1QK21|A0A1I1QK21_9FLAO MJ0570-related uncharacterized domain-containing protein OS=Algibacter pectinivorans OX=870482 GN=SAMN04487987_106226 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.06 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 8.0 KK42 pKa = 10.66 LSVSTEE48 pKa = 3.92 TRR50 pKa = 11.84 HH51 pKa = 6.44 KK52 pKa = 10.57 KK53 pKa = 9.81
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3126
0
3126
1081701
39
2748
346.0
39.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.381 ± 0.039
0.771 ± 0.016
5.627 ± 0.039
6.312 ± 0.044
5.282 ± 0.038
6.261 ± 0.045
1.768 ± 0.021
7.971 ± 0.049
8.016 ± 0.059
9.246 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.031 ± 0.024
6.69 ± 0.051
3.313 ± 0.026
3.251 ± 0.024
3.203 ± 0.028
6.482 ± 0.034
5.996 ± 0.053
6.26 ± 0.032
1.042 ± 0.016
4.098 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here