Hubei narna-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KIU3|A0A1L3KIU3_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 4 OX=1922958 PE=4 SV=1
MM1 pKa = 7.64FIFEE5 pKa = 4.64TFSGQCKK12 pKa = 8.64EE13 pKa = 4.22WQNLCHH19 pKa = 6.45SRR21 pKa = 11.84VKK23 pKa = 10.17EE24 pKa = 3.66ALRR27 pKa = 11.84IRR29 pKa = 11.84KK30 pKa = 8.62LPACWACHH38 pKa = 5.88FPSDD42 pKa = 5.2DD43 pKa = 3.59NPEE46 pKa = 4.02YY47 pKa = 10.56LQVVTSLFNSKK58 pKa = 9.95VEE60 pKa = 4.1EE61 pKa = 4.33KK62 pKa = 10.42DD63 pKa = 3.02WPKK66 pKa = 10.52YY67 pKa = 8.74IQWAIDD73 pKa = 3.47FSTRR77 pKa = 11.84LLEE80 pKa = 4.44SYY82 pKa = 8.96LTYY85 pKa = 10.74FEE87 pKa = 5.78LGLNRR92 pKa = 11.84VLPTDD97 pKa = 3.68RR98 pKa = 11.84KK99 pKa = 9.82ILRR102 pKa = 11.84KK103 pKa = 10.23VIFDD107 pKa = 3.94CLDD110 pKa = 3.11YY111 pKa = 10.99WFLLSLIGKK120 pKa = 8.46LPKK123 pKa = 9.57YY124 pKa = 10.18LKK126 pKa = 10.47YY127 pKa = 7.9WTCKK131 pKa = 9.58FFSDD135 pKa = 4.7ILQQFDD141 pKa = 3.61QPEE144 pKa = 4.22KK145 pKa = 10.89PEE147 pKa = 4.26IPLKK151 pKa = 10.42FKK153 pKa = 10.78PFICGQIAGKK163 pKa = 9.22IHH165 pKa = 7.44LITYY169 pKa = 8.64VRR171 pKa = 11.84TRR173 pKa = 11.84SDD175 pKa = 3.26RR176 pKa = 11.84KK177 pKa = 9.93PSEE180 pKa = 3.77DD181 pKa = 3.29HH182 pKa = 6.64IKK184 pKa = 10.73FFFGLLDD191 pKa = 3.71LKK193 pKa = 10.75KK194 pKa = 10.53ASRR197 pKa = 11.84PIHH200 pKa = 6.16KK201 pKa = 9.92IFVTEE206 pKa = 3.96EE207 pKa = 3.91VEE209 pKa = 4.43KK210 pKa = 10.95SIEE213 pKa = 4.01RR214 pKa = 11.84LTTSPEE220 pKa = 3.86RR221 pKa = 11.84EE222 pKa = 3.9PLRR225 pKa = 11.84NGIIRR230 pKa = 11.84SIKK233 pKa = 9.61RR234 pKa = 11.84VCNSLVFSHH243 pKa = 6.86IRR245 pKa = 11.84GNCSDD250 pKa = 3.86IPINLPSCLPTTGGLEE266 pKa = 3.91NPRR269 pKa = 11.84RR270 pKa = 11.84TGGCYY275 pKa = 10.42GFLQGNLSSLCNNISGSFHH294 pKa = 6.77LCNMEE299 pKa = 3.92KK300 pKa = 10.39DD301 pKa = 4.0GEE303 pKa = 4.2ILPVSVPFNPRR314 pKa = 11.84IEE316 pKa = 4.05LSYY319 pKa = 10.78YY320 pKa = 10.26RR321 pKa = 11.84HH322 pKa = 6.83LDD324 pKa = 3.9DD325 pKa = 5.99LSKK328 pKa = 9.66TYY330 pKa = 10.1PGSIPVEE337 pKa = 4.06MVGLPEE343 pKa = 4.12PFKK346 pKa = 11.32VRR348 pKa = 11.84TISKK352 pKa = 10.7GPIDD356 pKa = 5.32LYY358 pKa = 11.01AQARR362 pKa = 11.84RR363 pKa = 11.84YY364 pKa = 9.71QGLFWHH370 pKa = 7.31LLRR373 pKa = 11.84HH374 pKa = 5.66SPVFSLTWSPLCEE387 pKa = 3.6YY388 pKa = 10.23HH389 pKa = 7.34LRR391 pKa = 11.84YY392 pKa = 9.83LYY394 pKa = 10.77NGNPFSKK401 pKa = 10.49NRR403 pKa = 11.84LEE405 pKa = 4.49SYY407 pKa = 10.43LLSADD412 pKa = 3.72YY413 pKa = 10.42EE414 pKa = 4.21AATDD418 pKa = 4.02SIYY421 pKa = 11.09KK422 pKa = 9.96EE423 pKa = 4.26IIEE426 pKa = 4.28EE427 pKa = 4.12TAEE430 pKa = 4.16HH431 pKa = 6.98LFHH434 pKa = 7.68LLDD437 pKa = 4.31IPWSDD442 pKa = 3.2RR443 pKa = 11.84ALLLKK448 pKa = 10.28ILTRR452 pKa = 11.84CEE454 pKa = 3.48IEE456 pKa = 4.21RR457 pKa = 11.84LDD459 pKa = 4.94GEE461 pKa = 4.38SLKK464 pKa = 10.61QKK466 pKa = 10.17RR467 pKa = 11.84GQLMGSPLSFVLLCIINAGVNLFVIEE493 pKa = 4.14KK494 pKa = 10.5SIGEE498 pKa = 4.15RR499 pKa = 11.84IKK501 pKa = 10.82FPLFGEE507 pKa = 4.23EE508 pKa = 4.83VPFLVNGDD516 pKa = 4.53DD517 pKa = 3.92YY518 pKa = 11.94LSFLPVRR525 pKa = 11.84YY526 pKa = 9.6YY527 pKa = 10.25EE528 pKa = 4.02FWKK531 pKa = 10.77EE532 pKa = 4.03CVTSVGLKK540 pKa = 10.06PSLGKK545 pKa = 10.42NYY547 pKa = 9.58LHH549 pKa = 7.57PYY551 pKa = 9.49LGTVNSTLIKK561 pKa = 10.12INRR564 pKa = 11.84PLHH567 pKa = 5.13FDD569 pKa = 3.38EE570 pKa = 5.4RR571 pKa = 11.84FKK573 pKa = 11.71DD574 pKa = 3.7NLPGKK579 pKa = 9.94IFRR582 pKa = 11.84HH583 pKa = 5.54LKK585 pKa = 10.48LSLAYY590 pKa = 9.37PPEE593 pKa = 4.27EE594 pKa = 3.6QDD596 pKa = 4.77VLIKK600 pKa = 10.78DD601 pKa = 3.9DD602 pKa = 3.64TFDD605 pKa = 3.18WRR607 pKa = 11.84KK608 pKa = 10.08ISIKK612 pKa = 10.01TNLEE616 pKa = 3.76RR617 pKa = 11.84LILDD621 pKa = 3.26QKK623 pKa = 11.33EE624 pKa = 4.2DD625 pKa = 3.35IQRR628 pKa = 11.84TLIFYY633 pKa = 10.53ALRR636 pKa = 11.84KK637 pKa = 8.86FKK639 pKa = 10.77KK640 pKa = 9.3FLSVIPKK647 pKa = 8.32EE648 pKa = 4.08IPYY651 pKa = 10.27SVSPSLGGLGIPLVDD666 pKa = 4.01HH667 pKa = 6.47KK668 pKa = 11.08QVSDD672 pKa = 3.46PHH674 pKa = 7.63RR675 pKa = 11.84MRR677 pKa = 11.84VSYY680 pKa = 10.82LITRR684 pKa = 11.84PPKK687 pKa = 10.1RR688 pKa = 11.84GRR690 pKa = 11.84TLIFPGEE697 pKa = 4.2VSQVDD702 pKa = 3.97LLLKK706 pKa = 10.4EE707 pKa = 4.88KK708 pKa = 10.54EE709 pKa = 4.16NQLLDD714 pKa = 3.5QLEE717 pKa = 4.55IPWEE721 pKa = 4.42PGTEE725 pKa = 3.99KK726 pKa = 10.58PSPRR730 pKa = 11.84EE731 pKa = 3.82GEE733 pKa = 4.37SPFSRR738 pKa = 11.84WCFLYY743 pKa = 10.22EE744 pKa = 4.07FEE746 pKa = 5.09KK747 pKa = 10.66IKK749 pKa = 10.7PKK751 pKa = 10.77VSILQTLKK759 pKa = 9.63KK760 pKa = 8.33WSYY763 pKa = 9.61IIKK766 pKa = 10.28RR767 pKa = 11.84EE768 pKa = 4.02LKK770 pKa = 10.09EE771 pKa = 4.17SNRR774 pKa = 11.84LLKK777 pKa = 10.34VLRR780 pKa = 11.84PASSTSCKK788 pKa = 9.89QDD790 pKa = 3.13VKK792 pKa = 10.3PWTRR796 pKa = 11.84TFPLYY801 pKa = 10.62TFDD804 pKa = 5.62LGLGKK809 pKa = 10.25DD810 pKa = 3.91DD811 pKa = 4.08FVSRR815 pKa = 11.84AALFRR820 pKa = 11.84EE821 pKa = 4.09ITPGIDD827 pKa = 3.55LFII830 pKa = 6.14

Molecular weight: 96.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KIU3|A0A1L3KIU3_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 4 OX=1922958 PE=4 SV=1
MM1 pKa = 7.64FIFEE5 pKa = 4.64TFSGQCKK12 pKa = 8.64EE13 pKa = 4.22WQNLCHH19 pKa = 6.45SRR21 pKa = 11.84VKK23 pKa = 10.17EE24 pKa = 3.66ALRR27 pKa = 11.84IRR29 pKa = 11.84KK30 pKa = 8.62LPACWACHH38 pKa = 5.88FPSDD42 pKa = 5.2DD43 pKa = 3.59NPEE46 pKa = 4.02YY47 pKa = 10.56LQVVTSLFNSKK58 pKa = 9.95VEE60 pKa = 4.1EE61 pKa = 4.33KK62 pKa = 10.42DD63 pKa = 3.02WPKK66 pKa = 10.52YY67 pKa = 8.74IQWAIDD73 pKa = 3.47FSTRR77 pKa = 11.84LLEE80 pKa = 4.44SYY82 pKa = 8.96LTYY85 pKa = 10.74FEE87 pKa = 5.78LGLNRR92 pKa = 11.84VLPTDD97 pKa = 3.68RR98 pKa = 11.84KK99 pKa = 9.82ILRR102 pKa = 11.84KK103 pKa = 10.23VIFDD107 pKa = 3.94CLDD110 pKa = 3.11YY111 pKa = 10.99WFLLSLIGKK120 pKa = 8.46LPKK123 pKa = 9.57YY124 pKa = 10.18LKK126 pKa = 10.47YY127 pKa = 7.9WTCKK131 pKa = 9.58FFSDD135 pKa = 4.7ILQQFDD141 pKa = 3.61QPEE144 pKa = 4.22KK145 pKa = 10.89PEE147 pKa = 4.26IPLKK151 pKa = 10.42FKK153 pKa = 10.78PFICGQIAGKK163 pKa = 9.22IHH165 pKa = 7.44LITYY169 pKa = 8.64VRR171 pKa = 11.84TRR173 pKa = 11.84SDD175 pKa = 3.26RR176 pKa = 11.84KK177 pKa = 9.93PSEE180 pKa = 3.77DD181 pKa = 3.29HH182 pKa = 6.64IKK184 pKa = 10.73FFFGLLDD191 pKa = 3.71LKK193 pKa = 10.75KK194 pKa = 10.53ASRR197 pKa = 11.84PIHH200 pKa = 6.16KK201 pKa = 9.92IFVTEE206 pKa = 3.96EE207 pKa = 3.91VEE209 pKa = 4.43KK210 pKa = 10.95SIEE213 pKa = 4.01RR214 pKa = 11.84LTTSPEE220 pKa = 3.86RR221 pKa = 11.84EE222 pKa = 3.9PLRR225 pKa = 11.84NGIIRR230 pKa = 11.84SIKK233 pKa = 9.61RR234 pKa = 11.84VCNSLVFSHH243 pKa = 6.86IRR245 pKa = 11.84GNCSDD250 pKa = 3.86IPINLPSCLPTTGGLEE266 pKa = 3.91NPRR269 pKa = 11.84RR270 pKa = 11.84TGGCYY275 pKa = 10.42GFLQGNLSSLCNNISGSFHH294 pKa = 6.77LCNMEE299 pKa = 3.92KK300 pKa = 10.39DD301 pKa = 4.0GEE303 pKa = 4.2ILPVSVPFNPRR314 pKa = 11.84IEE316 pKa = 4.05LSYY319 pKa = 10.78YY320 pKa = 10.26RR321 pKa = 11.84HH322 pKa = 6.83LDD324 pKa = 3.9DD325 pKa = 5.99LSKK328 pKa = 9.66TYY330 pKa = 10.1PGSIPVEE337 pKa = 4.06MVGLPEE343 pKa = 4.12PFKK346 pKa = 11.32VRR348 pKa = 11.84TISKK352 pKa = 10.7GPIDD356 pKa = 5.32LYY358 pKa = 11.01AQARR362 pKa = 11.84RR363 pKa = 11.84YY364 pKa = 9.71QGLFWHH370 pKa = 7.31LLRR373 pKa = 11.84HH374 pKa = 5.66SPVFSLTWSPLCEE387 pKa = 3.6YY388 pKa = 10.23HH389 pKa = 7.34LRR391 pKa = 11.84YY392 pKa = 9.83LYY394 pKa = 10.77NGNPFSKK401 pKa = 10.49NRR403 pKa = 11.84LEE405 pKa = 4.49SYY407 pKa = 10.43LLSADD412 pKa = 3.72YY413 pKa = 10.42EE414 pKa = 4.21AATDD418 pKa = 4.02SIYY421 pKa = 11.09KK422 pKa = 9.96EE423 pKa = 4.26IIEE426 pKa = 4.28EE427 pKa = 4.12TAEE430 pKa = 4.16HH431 pKa = 6.98LFHH434 pKa = 7.68LLDD437 pKa = 4.31IPWSDD442 pKa = 3.2RR443 pKa = 11.84ALLLKK448 pKa = 10.28ILTRR452 pKa = 11.84CEE454 pKa = 3.48IEE456 pKa = 4.21RR457 pKa = 11.84LDD459 pKa = 4.94GEE461 pKa = 4.38SLKK464 pKa = 10.61QKK466 pKa = 10.17RR467 pKa = 11.84GQLMGSPLSFVLLCIINAGVNLFVIEE493 pKa = 4.14KK494 pKa = 10.5SIGEE498 pKa = 4.15RR499 pKa = 11.84IKK501 pKa = 10.82FPLFGEE507 pKa = 4.23EE508 pKa = 4.83VPFLVNGDD516 pKa = 4.53DD517 pKa = 3.92YY518 pKa = 11.94LSFLPVRR525 pKa = 11.84YY526 pKa = 9.6YY527 pKa = 10.25EE528 pKa = 4.02FWKK531 pKa = 10.77EE532 pKa = 4.03CVTSVGLKK540 pKa = 10.06PSLGKK545 pKa = 10.42NYY547 pKa = 9.58LHH549 pKa = 7.57PYY551 pKa = 9.49LGTVNSTLIKK561 pKa = 10.12INRR564 pKa = 11.84PLHH567 pKa = 5.13FDD569 pKa = 3.38EE570 pKa = 5.4RR571 pKa = 11.84FKK573 pKa = 11.71DD574 pKa = 3.7NLPGKK579 pKa = 9.94IFRR582 pKa = 11.84HH583 pKa = 5.54LKK585 pKa = 10.48LSLAYY590 pKa = 9.37PPEE593 pKa = 4.27EE594 pKa = 3.6QDD596 pKa = 4.77VLIKK600 pKa = 10.78DD601 pKa = 3.9DD602 pKa = 3.64TFDD605 pKa = 3.18WRR607 pKa = 11.84KK608 pKa = 10.08ISIKK612 pKa = 10.01TNLEE616 pKa = 3.76RR617 pKa = 11.84LILDD621 pKa = 3.26QKK623 pKa = 11.33EE624 pKa = 4.2DD625 pKa = 3.35IQRR628 pKa = 11.84TLIFYY633 pKa = 10.53ALRR636 pKa = 11.84KK637 pKa = 8.86FKK639 pKa = 10.77KK640 pKa = 9.3FLSVIPKK647 pKa = 8.32EE648 pKa = 4.08IPYY651 pKa = 10.27SVSPSLGGLGIPLVDD666 pKa = 4.01HH667 pKa = 6.47KK668 pKa = 11.08QVSDD672 pKa = 3.46PHH674 pKa = 7.63RR675 pKa = 11.84MRR677 pKa = 11.84VSYY680 pKa = 10.82LITRR684 pKa = 11.84PPKK687 pKa = 10.1RR688 pKa = 11.84GRR690 pKa = 11.84TLIFPGEE697 pKa = 4.2VSQVDD702 pKa = 3.97LLLKK706 pKa = 10.4EE707 pKa = 4.88KK708 pKa = 10.54EE709 pKa = 4.16NQLLDD714 pKa = 3.5QLEE717 pKa = 4.55IPWEE721 pKa = 4.42PGTEE725 pKa = 3.99KK726 pKa = 10.58PSPRR730 pKa = 11.84EE731 pKa = 3.82GEE733 pKa = 4.37SPFSRR738 pKa = 11.84WCFLYY743 pKa = 10.22EE744 pKa = 4.07FEE746 pKa = 5.09KK747 pKa = 10.66IKK749 pKa = 10.7PKK751 pKa = 10.77VSILQTLKK759 pKa = 9.63KK760 pKa = 8.33WSYY763 pKa = 9.61IIKK766 pKa = 10.28RR767 pKa = 11.84EE768 pKa = 4.02LKK770 pKa = 10.09EE771 pKa = 4.17SNRR774 pKa = 11.84LLKK777 pKa = 10.34VLRR780 pKa = 11.84PASSTSCKK788 pKa = 9.89QDD790 pKa = 3.13VKK792 pKa = 10.3PWTRR796 pKa = 11.84TFPLYY801 pKa = 10.62TFDD804 pKa = 5.62LGLGKK809 pKa = 10.25DD810 pKa = 3.91DD811 pKa = 4.08FVSRR815 pKa = 11.84AALFRR820 pKa = 11.84EE821 pKa = 4.09ITPGIDD827 pKa = 3.55LFII830 pKa = 6.14

Molecular weight: 96.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

830

830

830

830.0

96.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.289 ± 0.0

2.289 ± 0.0

4.94 ± 0.0

6.988 ± 0.0

6.024 ± 0.0

5.06 ± 0.0

2.169 ± 0.0

7.47 ± 0.0

7.831 ± 0.0

13.012 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.602 ± 0.0

3.253 ± 0.0

6.988 ± 0.0

2.651 ± 0.0

6.386 ± 0.0

7.59 ± 0.0

4.217 ± 0.0

4.578 ± 0.0

1.807 ± 0.0

3.855 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski