Mirim virus
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L5ITC5|A0A1L5ITC5_9VIRU Envelopment polyprotein OS=Mirim virus OX=1927814 PE=4 SV=1
MM1 pKa = 7.93 AILIEE6 pKa = 4.98 DD7 pKa = 4.18 IVRR10 pKa = 11.84 QYY12 pKa = 8.9 QARR15 pKa = 11.84 IRR17 pKa = 11.84 ACTDD21 pKa = 3.05 PEE23 pKa = 3.88 IGRR26 pKa = 11.84 DD27 pKa = 3.17 ILTEE31 pKa = 3.71 ITVARR36 pKa = 11.84 HH37 pKa = 5.5 NYY39 pKa = 8.11 FAQEE43 pKa = 4.09 FCASIDD49 pKa = 3.42 LEE51 pKa = 4.22 YY52 pKa = 11.12 RR53 pKa = 11.84 NDD55 pKa = 3.45 VPALDD60 pKa = 3.95 IVMEE64 pKa = 4.17 MVPDD68 pKa = 4.49 FDD70 pKa = 3.57 PTAIYY75 pKa = 9.88 VPNVTPDD82 pKa = 2.57 NFMRR86 pKa = 11.84 DD87 pKa = 3.41 GNKK90 pKa = 10.37 LYY92 pKa = 10.33 IIDD95 pKa = 4.33 FKK97 pKa = 11.67 VSVSDD102 pKa = 3.47 EE103 pKa = 4.59 SSNHH107 pKa = 4.94 TYY109 pKa = 10.76 KK110 pKa = 10.87 KK111 pKa = 8.55 YY112 pKa = 7.29 TTIFGDD118 pKa = 3.42 IFNQLGVEE126 pKa = 4.04 FEE128 pKa = 4.43 VVIIRR133 pKa = 11.84 MNPSNMHH140 pKa = 5.83 LTISSDD146 pKa = 3.59 QFAHH150 pKa = 7.35 LYY152 pKa = 9.76 PNIILNLDD160 pKa = 3.8 FSWYY164 pKa = 9.76 FRR166 pKa = 11.84 LKK168 pKa = 10.14 EE169 pKa = 3.74 ALFTKK174 pKa = 10.43 FQDD177 pKa = 3.42 NEE179 pKa = 3.93 EE180 pKa = 4.01 FMEE183 pKa = 4.37 LVAHH187 pKa = 6.95 GEE189 pKa = 4.2 FTPTFPWVTEE199 pKa = 3.9 PTFEE203 pKa = 5.25 LMQDD207 pKa = 3.85 PIFIEE212 pKa = 5.53 FIEE215 pKa = 4.58 SMDD218 pKa = 5.17 AEE220 pKa = 4.34 HH221 pKa = 7.1 QDD223 pKa = 3.63 DD224 pKa = 5.82 FFYY227 pKa = 11.32 ALNNNAFQSDD237 pKa = 3.25 KK238 pKa = 10.55 WNDD241 pKa = 3.44 MLHH244 pKa = 6.18 MFMQKK249 pKa = 10.21 YY250 pKa = 6.68 SQKK253 pKa = 10.43 YY254 pKa = 7.77 KK255 pKa = 11.07 AFVSKK260 pKa = 9.3 MAKK263 pKa = 9.68 EE264 pKa = 3.7 IFIIDD269 pKa = 3.74 EE270 pKa = 4.25 NFQKK274 pKa = 9.53 PTSRR278 pKa = 11.84 EE279 pKa = 3.43 ITLGWNEE286 pKa = 3.79 MIKK289 pKa = 10.35 RR290 pKa = 11.84 VGEE293 pKa = 3.95 MRR295 pKa = 11.84 NVTKK299 pKa = 10.37 DD300 pKa = 3.16 VHH302 pKa = 5.56 KK303 pKa = 10.27 QKK305 pKa = 10.7 PSIHH309 pKa = 6.58 FIWSPHH315 pKa = 6.19 DD316 pKa = 3.75 YY317 pKa = 10.63 KK318 pKa = 11.54 SPVDD322 pKa = 3.73 NNKK325 pKa = 10.42 KK326 pKa = 10.04 IIRR329 pKa = 11.84 LAQALSSIKK338 pKa = 10.91 DD339 pKa = 3.48 KK340 pKa = 11.24 DD341 pKa = 3.65 NHH343 pKa = 6.29 SLAFKK348 pKa = 10.83 YY349 pKa = 10.06 IGEE352 pKa = 4.25 LMDD355 pKa = 4.18 FSSDD359 pKa = 2.58 ISKK362 pKa = 11.05 YY363 pKa = 10.42 EE364 pKa = 3.84 NFCISLKK371 pKa = 10.44 AAARR375 pKa = 11.84 SSIKK379 pKa = 10.36 KK380 pKa = 9.79 KK381 pKa = 10.62 SEE383 pKa = 3.69 QIKK386 pKa = 9.99 PITIGKK392 pKa = 8.06 CTILWEE398 pKa = 4.22 QQFKK402 pKa = 10.76 LDD404 pKa = 3.6 SDD406 pKa = 4.81 IIPKK410 pKa = 9.98 EE411 pKa = 3.66 IRR413 pKa = 11.84 LKK415 pKa = 10.51 FLKK418 pKa = 9.52 EE419 pKa = 3.52 FCGIGGHH426 pKa = 5.36 KK427 pKa = 9.29 QFKK430 pKa = 10.58 NRR432 pKa = 11.84 MLDD435 pKa = 3.89 DD436 pKa = 5.21 LDD438 pKa = 4.5 LEE440 pKa = 4.88 KK441 pKa = 9.8 PTILDD446 pKa = 3.55 FSDD449 pKa = 3.46 IEE451 pKa = 4.52 IIRR454 pKa = 11.84 HH455 pKa = 4.39 AQIMVTDD462 pKa = 4.15 SKK464 pKa = 11.83 SFLSKK469 pKa = 10.69 KK470 pKa = 10.5 SGLTKK475 pKa = 10.15 IGNTLEE481 pKa = 4.13 DD482 pKa = 4.31 FKK484 pKa = 11.88 DD485 pKa = 3.67 KK486 pKa = 10.79 ILNANEE492 pKa = 4.09 NTWKK496 pKa = 10.27 IIEE499 pKa = 4.97 EE500 pKa = 3.88 ISKK503 pKa = 8.57 TRR505 pKa = 11.84 FWQCINDD512 pKa = 3.12 ISTLVKK518 pKa = 10.73 NILSVSQYY526 pKa = 10.54 NKK528 pKa = 9.68 HH529 pKa = 4.01 NTFRR533 pKa = 11.84 VVTSANNNFFGLVYY547 pKa = 9.34 PASSIQSKK555 pKa = 10.08 KK556 pKa = 8.66 ATIVFSTVTLHH567 pKa = 7.24 KK568 pKa = 10.75 NEE570 pKa = 4.6 KK571 pKa = 10.21 DD572 pKa = 3.57 VLNCGSLFKK581 pKa = 10.18 TYY583 pKa = 10.44 RR584 pKa = 11.84 IGAGFISISKK594 pKa = 10.37 AIRR597 pKa = 11.84 LDD599 pKa = 3.32 KK600 pKa = 10.58 EE601 pKa = 3.73 RR602 pKa = 11.84 CQRR605 pKa = 11.84 LVISPGIFLLSALLFKK621 pKa = 10.92 GDD623 pKa = 3.9 SDD625 pKa = 5.12 VEE627 pKa = 4.2 LTDD630 pKa = 3.15 ILIFVFFTSLSITKK644 pKa = 10.46 SMLSLTEE651 pKa = 3.7 PSRR654 pKa = 11.84 YY655 pKa = 9.4 MIMNSLALSSHH666 pKa = 3.86 VRR668 pKa = 11.84 EE669 pKa = 4.38 YY670 pKa = 10.57 ISEE673 pKa = 4.16 KK674 pKa = 9.97 FSPYY678 pKa = 9.81 TKK680 pKa = 9.97 TLFSVYY686 pKa = 7.18 MTEE689 pKa = 5.43 LIRR692 pKa = 11.84 KK693 pKa = 9.03 GCMSANNQRR702 pKa = 11.84 HH703 pKa = 6.03 KK704 pKa = 10.85 ISVRR708 pKa = 11.84 DD709 pKa = 3.49 IFLNEE714 pKa = 3.74 YY715 pKa = 10.26 EE716 pKa = 4.18 ITQKK720 pKa = 10.84 GVSDD724 pKa = 3.85 NKK726 pKa = 10.58 DD727 pKa = 3.34 LEE729 pKa = 5.08 SIWFPGNVGLKK740 pKa = 10.3 DD741 pKa = 4.15 YY742 pKa = 10.79 INQVYY747 pKa = 10.28 LPFYY751 pKa = 10.39 FNAKK755 pKa = 9.15 GLHH758 pKa = 5.76 NKK760 pKa = 8.23 HH761 pKa = 6.18 HH762 pKa = 6.02 VMIDD766 pKa = 3.33 LAKK769 pKa = 9.56 TILEE773 pKa = 4.08 IEE775 pKa = 4.18 LEE777 pKa = 4.16 QRR779 pKa = 11.84 EE780 pKa = 4.57 EE781 pKa = 4.64 LPNPWGDD788 pKa = 3.37 KK789 pKa = 10.52 FEE791 pKa = 4.34 KK792 pKa = 10.51 QSVNLDD798 pKa = 3.09 ILIYY802 pKa = 10.51 SISKK806 pKa = 8.77 MLNNDD811 pKa = 3.38 TSKK814 pKa = 10.92 HH815 pKa = 3.74 NHH817 pKa = 4.86 LRR819 pKa = 11.84 SRR821 pKa = 11.84 IEE823 pKa = 3.44 NRR825 pKa = 11.84 NNFKK829 pKa = 10.74 RR830 pKa = 11.84 SLTSISTFTSSKK842 pKa = 10.34 SCIKK846 pKa = 10.59 VGNFYY851 pKa = 10.7 DD852 pKa = 5.08 LKK854 pKa = 11.1 DD855 pKa = 3.73 AMSKK859 pKa = 10.47 KK860 pKa = 9.6 IRR862 pKa = 11.84 KK863 pKa = 8.12 QLEE866 pKa = 3.85 KK867 pKa = 10.03 EE868 pKa = 4.06 AKK870 pKa = 7.71 KK871 pKa = 9.07 TRR873 pKa = 11.84 IANTEE878 pKa = 3.85 FVNEE882 pKa = 4.04 SDD884 pKa = 3.66 RR885 pKa = 11.84 DD886 pKa = 3.8 LEE888 pKa = 4.5 VAHH891 pKa = 6.16 CTYY894 pKa = 11.32 SDD896 pKa = 3.73 LVKK899 pKa = 10.66 SIPHH903 pKa = 4.93 YY904 pKa = 9.36 TDD906 pKa = 3.07 YY907 pKa = 11.11 MSTKK911 pKa = 10.06 VFDD914 pKa = 3.87 RR915 pKa = 11.84 LYY917 pKa = 10.81 EE918 pKa = 3.94 KK919 pKa = 10.7 FKK921 pKa = 10.19 TKK923 pKa = 10.1 EE924 pKa = 4.03 YY925 pKa = 9.88 EE926 pKa = 4.12 DD927 pKa = 4.11 KK928 pKa = 10.91 PSIEE932 pKa = 4.57 IIMDD936 pKa = 3.11 VMKK939 pKa = 10.47 NHH941 pKa = 6.59 KK942 pKa = 9.71 NFRR945 pKa = 11.84 FCFFNKK951 pKa = 9.34 GQKK954 pKa = 6.26 TAKK957 pKa = 10.03 DD958 pKa = 3.32 RR959 pKa = 11.84 EE960 pKa = 4.35 IFVGEE965 pKa = 4.5 LEE967 pKa = 4.71 SKK969 pKa = 10.47 LCLYY973 pKa = 10.31 CVEE976 pKa = 5.19 RR977 pKa = 11.84 IAKK980 pKa = 7.76 EE981 pKa = 3.89 RR982 pKa = 11.84 CKK984 pKa = 10.8 LNPDD988 pKa = 3.47 EE989 pKa = 5.2 MISEE993 pKa = 4.56 PGDD996 pKa = 3.55 GKK998 pKa = 10.93 LKK1000 pKa = 10.49 KK1001 pKa = 10.59 LEE1003 pKa = 4.13 INAEE1007 pKa = 3.94 SEE1009 pKa = 3.54 IRR1011 pKa = 11.84 YY1012 pKa = 9.11 LIEE1015 pKa = 3.59 MTRR1018 pKa = 11.84 KK1019 pKa = 7.85 KK1020 pKa = 9.24 TKK1022 pKa = 10.18 EE1023 pKa = 3.69 EE1024 pKa = 3.95 EE1025 pKa = 4.17 FLSKK1029 pKa = 10.27 LTDD1032 pKa = 3.38 NPKK1035 pKa = 10.54 GIKK1038 pKa = 10.29 LEE1040 pKa = 4.28 INADD1044 pKa = 3.48 MSKK1047 pKa = 10.21 WSAQDD1052 pKa = 3.37 VFFKK1056 pKa = 10.47 YY1057 pKa = 10.17 FWLIALDD1064 pKa = 4.41 PILYY1068 pKa = 9.38 PFEE1071 pKa = 4.17 KK1072 pKa = 10.34 QRR1074 pKa = 11.84 IIYY1077 pKa = 7.38 FMCNYY1082 pKa = 7.71 MKK1084 pKa = 10.66 KK1085 pKa = 10.23 EE1086 pKa = 4.23 LILPDD1091 pKa = 4.0 EE1092 pKa = 4.6 MMCSLLDD1099 pKa = 3.46 QRR1101 pKa = 11.84 QSRR1104 pKa = 11.84 EE1105 pKa = 3.41 QDD1107 pKa = 2.92 IIKK1110 pKa = 10.56 QMTDD1114 pKa = 2.65 NFSKK1118 pKa = 9.55 NTVNIKK1124 pKa = 10.61 RR1125 pKa = 11.84 NWLQGNLNYY1134 pKa = 9.69 TSSYY1138 pKa = 9.82 IHH1140 pKa = 6.58 SCSMMVFKK1148 pKa = 10.72 DD1149 pKa = 3.45 IIKK1152 pKa = 8.03 EE1153 pKa = 4.23 TSSLLEE1159 pKa = 3.98 GSCNVSSLVHH1169 pKa = 6.91 SDD1171 pKa = 4.03 DD1172 pKa = 3.76 NQTSVIIIQDD1182 pKa = 3.38 KK1183 pKa = 9.7 LHH1185 pKa = 6.86 NDD1187 pKa = 4.26 FIINFVCNLFEE1198 pKa = 4.49 ACCLTFGNQANMKK1211 pKa = 8.16 KK1212 pKa = 8.88 TYY1214 pKa = 8.14 VTNNIKK1220 pKa = 10.67 EE1221 pKa = 4.24 FVSLFNIYY1229 pKa = 10.55 GEE1231 pKa = 4.16 PFSVYY1236 pKa = 10.58 GRR1238 pKa = 11.84 FLLPAVGDD1246 pKa = 3.79 CAYY1249 pKa = 9.78 IGPYY1253 pKa = 10.2 EE1254 pKa = 4.5 DD1255 pKa = 3.13 MASRR1259 pKa = 11.84 LSATQTAIKK1268 pKa = 9.76 HH1269 pKa = 5.3 GCPPSLAWVSIALNHH1284 pKa = 7.22 WITFSTYY1291 pKa = 11.2 NMLPGQQNDD1300 pKa = 3.45 PTKK1303 pKa = 10.91 VFNCSRR1309 pKa = 11.84 EE1310 pKa = 3.89 EE1311 pKa = 4.62 LPIEE1315 pKa = 3.9 LCGILKK1321 pKa = 10.33 SDD1323 pKa = 4.33 LSTIALVGLEE1333 pKa = 4.18 AGNISFLTSLLKK1345 pKa = 10.84 KK1346 pKa = 9.83 MSPPQLTKK1354 pKa = 10.92 EE1355 pKa = 4.3 SVQTQCNNIKK1365 pKa = 10.27 NWDD1368 pKa = 3.93 LNKK1371 pKa = 9.12 LTRR1374 pKa = 11.84 MEE1376 pKa = 4.0 IFRR1379 pKa = 11.84 LKK1381 pKa = 9.23 ILRR1384 pKa = 11.84 YY1385 pKa = 9.1 IVLDD1389 pKa = 3.77 TEE1391 pKa = 4.4 LSEE1394 pKa = 4.52 DD1395 pKa = 3.4 STMGEE1400 pKa = 4.03 TSEE1403 pKa = 3.91 MRR1405 pKa = 11.84 SRR1407 pKa = 11.84 SLITPRR1413 pKa = 11.84 KK1414 pKa = 7.48 FTTASSLNRR1423 pKa = 11.84 LISYY1427 pKa = 10.48 KK1428 pKa = 10.46 DD1429 pKa = 3.62 YY1430 pKa = 11.33 QDD1432 pKa = 4.61 VISNPTEE1439 pKa = 4.25 LDD1441 pKa = 3.39 SLLQDD1446 pKa = 3.69 MLDD1449 pKa = 3.67 HH1450 pKa = 7.3 QEE1452 pKa = 4.05 LLVTKK1457 pKa = 10.48 GEE1459 pKa = 4.11 TSEE1462 pKa = 4.21 EE1463 pKa = 3.87 FRR1465 pKa = 11.84 KK1466 pKa = 8.82 TILFRR1471 pKa = 11.84 FNSKK1475 pKa = 9.78 KK1476 pKa = 10.47 FKK1478 pKa = 10.37 EE1479 pKa = 4.3 SLSIQSPTQLFIEE1492 pKa = 4.64 QILFSNKK1499 pKa = 8.1 PTIDD1503 pKa = 3.22 YY1504 pKa = 11.16 SGIQDD1509 pKa = 3.94 RR1510 pKa = 11.84 YY1511 pKa = 10.87 LGLLDD1516 pKa = 4.08 IPAIQEE1522 pKa = 3.87 NEE1524 pKa = 4.26 GILGKK1529 pKa = 8.92 KK1530 pKa = 8.01 TIPEE1534 pKa = 4.28 TFGSLLEE1541 pKa = 4.58 DD1542 pKa = 4.13 LSNFTITHH1550 pKa = 6.16 EE1551 pKa = 4.64 DD1552 pKa = 2.87 IKK1554 pKa = 10.93 LVYY1557 pKa = 10.1 SFCILNDD1564 pKa = 3.93 PLNTTACNALLLSQIQSLMAKK1585 pKa = 8.86 TSLSAVTMPEE1595 pKa = 3.74 FRR1597 pKa = 11.84 NMKK1600 pKa = 9.54 LIKK1603 pKa = 10.15 YY1604 pKa = 9.86 SPALVLRR1611 pKa = 11.84 AYY1613 pKa = 10.77 LHH1615 pKa = 6.88 GDD1617 pKa = 3.62 LEE1619 pKa = 4.68 VGGASEE1625 pKa = 4.07 DD1626 pKa = 3.24 AMRR1629 pKa = 11.84 RR1630 pKa = 11.84 DD1631 pKa = 3.44 IYY1633 pKa = 11.03 HH1634 pKa = 6.62 LEE1636 pKa = 4.03 EE1637 pKa = 5.16 FIKK1640 pKa = 9.34 QTGILQRR1647 pKa = 11.84 LEE1649 pKa = 3.99 TRR1651 pKa = 11.84 IEE1653 pKa = 3.73 EE1654 pKa = 4.35 HH1655 pKa = 6.59 NRR1657 pKa = 11.84 LTGQQDD1663 pKa = 3.78 LLFEE1667 pKa = 4.19 IRR1669 pKa = 11.84 EE1670 pKa = 4.18 RR1671 pKa = 11.84 TKK1673 pKa = 10.69 FLQTCYY1679 pKa = 10.84 DD1680 pKa = 3.97 YY1681 pKa = 11.23 IKK1683 pKa = 10.11 STEE1686 pKa = 4.04 HH1687 pKa = 5.71 KK1688 pKa = 9.45 VKK1690 pKa = 10.8 VFILPSRR1697 pKa = 11.84 PYY1699 pKa = 9.84 TAFDD1703 pKa = 3.82 FCATIHH1709 pKa = 6.54 GNLIKK1714 pKa = 10.81 DD1715 pKa = 3.57 KK1716 pKa = 10.51 GWFSVHH1722 pKa = 5.58 YY1723 pKa = 9.51 LKK1725 pKa = 10.94 QIVSGSGKK1733 pKa = 10.42 ALVNQTPASEE1743 pKa = 4.16 QINMDD1748 pKa = 3.2 EE1749 pKa = 4.38 CFRR1752 pKa = 11.84 LIAHH1756 pKa = 6.79 FCDD1759 pKa = 3.45 TFIEE1763 pKa = 4.42 KK1764 pKa = 9.83 TSRR1767 pKa = 11.84 VSFLDD1772 pKa = 4.16 NILKK1776 pKa = 10.63 NYY1778 pKa = 7.18 TYY1780 pKa = 11.28 KK1781 pKa = 10.85 NLPVKK1786 pKa = 10.19 QLYY1789 pKa = 8.96 NQLINSPTKK1798 pKa = 9.8 RR1799 pKa = 11.84 QHH1801 pKa = 6.15 FLPLLFYY1808 pKa = 10.44 TKK1810 pKa = 10.64 EE1811 pKa = 4.0 LTQSDD1816 pKa = 4.61 LDD1818 pKa = 3.89 RR1819 pKa = 11.84 FDD1821 pKa = 4.58 AYY1823 pKa = 9.94 KK1824 pKa = 10.14 SHH1826 pKa = 6.89 EE1827 pKa = 4.09 RR1828 pKa = 11.84 VTWNDD1833 pKa = 2.87 WQVNRR1838 pKa = 11.84 DD1839 pKa = 3.58 MSTGAIDD1846 pKa = 3.87 LTIKK1850 pKa = 10.75 GYY1852 pKa = 8.7 MRR1854 pKa = 11.84 SIRR1857 pKa = 11.84 ILGEE1861 pKa = 4.08 DD1862 pKa = 4.14 DD1863 pKa = 3.62 GLKK1866 pKa = 9.48 IAEE1869 pKa = 4.3 MEE1871 pKa = 5.03 LIVGDD1876 pKa = 3.68 NTPIEE1881 pKa = 4.17 SHH1883 pKa = 5.97 GRR1885 pKa = 11.84 KK1886 pKa = 9.24 LLNTRR1891 pKa = 11.84 HH1892 pKa = 5.3 NLKK1895 pKa = 10.4 LEE1897 pKa = 4.02 RR1898 pKa = 11.84 MKK1900 pKa = 10.49 KK1901 pKa = 9.27 IQVLEE1906 pKa = 3.89 PNMYY1910 pKa = 10.5 YY1911 pKa = 8.87 ITWQKK1916 pKa = 10.9 KK1917 pKa = 7.19 SRR1919 pKa = 11.84 NTFTYY1924 pKa = 10.32 QILLSDD1930 pKa = 4.6 IIANRR1935 pKa = 11.84 NKK1937 pKa = 10.03 QSFSITGSKK1946 pKa = 10.37 YY1947 pKa = 9.34 NEE1949 pKa = 4.42 LVPVCPVLISRR1960 pKa = 11.84 IKK1962 pKa = 10.18 SVEE1965 pKa = 3.94 KK1966 pKa = 10.55 IKK1968 pKa = 10.73 ISAIRR1973 pKa = 11.84 HH1974 pKa = 5.48 LNQDD1978 pKa = 3.5 YY1979 pKa = 11.11 NLTKK1983 pKa = 10.84 LKK1985 pKa = 9.07 ITQNDD1990 pKa = 3.29 FAVIKK1995 pKa = 9.85 RR1996 pKa = 11.84 CHH1998 pKa = 5.68 FSKK2001 pKa = 10.42 MMFFYY2006 pKa = 10.77 GPSFVVGNIDD2016 pKa = 3.13 IAKK2019 pKa = 10.57 LMTTPSLLTVNYY2031 pKa = 9.07 PSLCQIPLITLAQIFNCNGDD2051 pKa = 3.71 NQDD2054 pKa = 3.19 LDD2056 pKa = 3.81 EE2057 pKa = 5.77 FEE2059 pKa = 4.65 FLSDD2063 pKa = 4.0 EE2064 pKa = 4.06 LLEE2067 pKa = 5.37 DD2068 pKa = 3.67 IEE2070 pKa = 5.07 SEE2072 pKa = 4.63 VVNSIPFFTVSYY2084 pKa = 10.36 ASKK2087 pKa = 10.09 SKK2089 pKa = 10.49 KK2090 pKa = 8.3 GHH2092 pKa = 4.71 TYY2094 pKa = 10.08 KK2095 pKa = 10.76 RR2096 pKa = 11.84 ALQEE2100 pKa = 3.9 ALRR2103 pKa = 11.84 SGLTEE2108 pKa = 4.49 LEE2110 pKa = 4.44 SEE2112 pKa = 5.29 LDD2114 pKa = 3.54 FCKK2117 pKa = 10.61 LGFFSSKK2124 pKa = 10.12 NVAILSTLVSVANRR2138 pKa = 11.84 LNMNEE2143 pKa = 3.8 WSTLLIKK2150 pKa = 9.99 CIHH2153 pKa = 6.1 MSFFNNNKK2161 pKa = 10.16 DD2162 pKa = 3.59 EE2163 pKa = 4.6 LFHH2166 pKa = 7.21 KK2167 pKa = 10.37 FRR2169 pKa = 11.84 IPKK2172 pKa = 10.13 AFLKK2176 pKa = 10.35 EE2177 pKa = 4.06 IIGEE2181 pKa = 4.23 VVDD2184 pKa = 3.74 WQKK2187 pKa = 11.33 VKK2189 pKa = 11.09 LFIDD2193 pKa = 5.1 GIEE2196 pKa = 4.61 AINPDD2201 pKa = 3.83 SHH2203 pKa = 6.2 WGQMLGHH2210 pKa = 6.99 FKK2212 pKa = 10.25 TKK2214 pKa = 10.62 AKK2216 pKa = 10.39 LAIDD2220 pKa = 4.63 LEE2222 pKa = 4.57 LKK2224 pKa = 10.95 LEE2226 pKa = 4.4 GSTWGEE2232 pKa = 3.91 MLDD2235 pKa = 4.1 MIDD2238 pKa = 3.61 EE2239 pKa = 4.5 FRR2241 pKa = 11.84 DD2242 pKa = 2.92 EE2243 pKa = 6.82 DD2244 pKa = 3.65 MFQFNN2249 pKa = 4.19
Molecular weight: 260.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.314
IPC2_protein 6.389
IPC_protein 6.415
Toseland 6.576
ProMoST 6.854
Dawson 6.81
Bjellqvist 6.839
Wikipedia 6.81
Rodwell 6.81
Grimsley 6.693
Solomon 6.81
Lehninger 6.81
Nozaki 7.132
DTASelect 7.22
Thurlkill 7.249
EMBOSS 7.22
Sillero 7.22
Patrickios 4.355
IPC_peptide 6.825
IPC2_peptide 6.839
IPC2.peptide.svr19 6.821
Protein with the highest isoelectric point:
>tr|A0A1L5ITD8|A0A1L5ITD8_9VIRU Nucleocapsid protein OS=Mirim virus OX=1927814 PE=3 SV=1
MM1 pKa = 7.83 AEE3 pKa = 4.24 QIPEE7 pKa = 3.81 IEE9 pKa = 4.33 FEE11 pKa = 4.46 EE12 pKa = 4.79 KK13 pKa = 10.62 SIAAGTNLFVPDD25 pKa = 3.98 DD26 pKa = 4.22 GYY28 pKa = 11.77 AYY30 pKa = 10.47 FLNEE34 pKa = 3.98 YY35 pKa = 10.09 SSQINFDD42 pKa = 4.1 SIRR45 pKa = 11.84 IFYY48 pKa = 10.58 LRR50 pKa = 11.84 AKK52 pKa = 9.79 DD53 pKa = 3.21 AKK55 pKa = 10.55 LRR57 pKa = 11.84 LRR59 pKa = 11.84 TTRR62 pKa = 11.84 AKK64 pKa = 10.62 VATLKK69 pKa = 10.6 FGSMKK74 pKa = 10.84 LMIVNNHH81 pKa = 6.22 HH82 pKa = 7.59 PDD84 pKa = 3.45 NEE86 pKa = 4.31 ATDD89 pKa = 4.61 LQPQDD94 pKa = 3.62 LTLHH98 pKa = 5.99 RR99 pKa = 11.84 VSGYY103 pKa = 8.52 LARR106 pKa = 11.84 YY107 pKa = 8.73 LKK109 pKa = 10.9 EE110 pKa = 4.1 KK111 pKa = 8.41 MTTNTAARR119 pKa = 11.84 IEE121 pKa = 4.09 IHH123 pKa = 6.58 SKK125 pKa = 9.38 IVNPIAEE132 pKa = 4.54 SVGITWTTGDD142 pKa = 4.72 DD143 pKa = 3.27 IYY145 pKa = 11.59 LSFFPGSEE153 pKa = 3.79 MFLEE157 pKa = 4.45 VFKK160 pKa = 10.38 MLPLAIGIFRR170 pKa = 11.84 VQQSQMKK177 pKa = 10.02 PEE179 pKa = 3.98 FLKK182 pKa = 10.77 KK183 pKa = 9.75 HH184 pKa = 5.91 LRR186 pKa = 11.84 QQYY189 pKa = 10.1 GDD191 pKa = 3.54 IPASQWMISMKK202 pKa = 10.77 DD203 pKa = 3.51 DD204 pKa = 3.25 IKK206 pKa = 11.41 NAVALVSKK214 pKa = 9.61 LPWGKK219 pKa = 10.59 AGLSAAARR227 pKa = 11.84 SFLGEE232 pKa = 3.95 FGIKK236 pKa = 9.16 II237 pKa = 3.72
Molecular weight: 26.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.366
IPC2_protein 8.317
IPC_protein 8.331
Toseland 9.033
ProMoST 8.741
Dawson 9.282
Bjellqvist 8.916
Wikipedia 9.443
Rodwell 9.516
Grimsley 9.355
Solomon 9.385
Lehninger 9.355
Nozaki 8.946
DTASelect 8.946
Thurlkill 9.092
EMBOSS 9.428
Sillero 9.18
Patrickios 4.838
IPC_peptide 9.385
IPC2_peptide 7.468
IPC2.peptide.svr19 7.588
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
3924
237
2249
1308.0
149.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.893 ± 1.017
2.829 ± 1.121
5.377 ± 0.463
6.626 ± 0.181
5.556 ± 0.427
4.23 ± 0.551
2.294 ± 0.072
8.614 ± 0.206
8.563 ± 0.223
9.531 ± 0.284
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.905 ± 0.196
5.632 ± 0.464
3.186 ± 0.216
3.211 ± 0.429
3.593 ± 0.446
7.645 ± 0.351
6.193 ± 0.432
4.587 ± 0.236
0.841 ± 0.209
3.695 ± 0.294
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here