Amino acid dipepetide frequency for Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.783AlaAla: 11.783 ± 0.172
0.985AlaCys: 0.985 ± 0.037
5.336AlaAsp: 5.336 ± 0.088
6.465AlaGlu: 6.465 ± 0.105
3.621AlaPhe: 3.621 ± 0.072
8.32AlaGly: 8.32 ± 0.147
2.26AlaHis: 2.26 ± 0.061
5.991AlaIle: 5.991 ± 0.075
4.1AlaLys: 4.1 ± 0.082
12.122AlaLeu: 12.122 ± 0.159
3.082AlaMet: 3.082 ± 0.061
3.343AlaAsn: 3.343 ± 0.067
3.965AlaPro: 3.965 ± 0.073
4.419AlaGln: 4.419 ± 0.078
6.162AlaArg: 6.162 ± 0.11
6.267AlaSer: 6.267 ± 0.097
4.901AlaThr: 4.901 ± 0.093
7.142AlaVal: 7.142 ± 0.11
1.425AlaTrp: 1.425 ± 0.046
2.761AlaTyr: 2.761 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
0.734CysAla: 0.734 ± 0.03
0.133CysCys: 0.133 ± 0.013
0.451CysAsp: 0.451 ± 0.024
0.437CysGlu: 0.437 ± 0.022
0.281CysPhe: 0.281 ± 0.021
0.796CysGly: 0.796 ± 0.039
0.264CysHis: 0.264 ± 0.017
0.442CysIle: 0.442 ± 0.023
0.289CysLys: 0.289 ± 0.018
0.812CysLeu: 0.812 ± 0.036
0.228CysMet: 0.228 ± 0.015
0.293CysAsn: 0.293 ± 0.017
0.458CysPro: 0.458 ± 0.023
0.313CysGln: 0.313 ± 0.019
0.49CysArg: 0.49 ± 0.022
0.521CysSer: 0.521 ± 0.026
0.373CysThr: 0.373 ± 0.022
0.563CysVal: 0.563 ± 0.026
0.109CysTrp: 0.109 ± 0.011
0.211CysTyr: 0.211 ± 0.018
0.0CysXaa: 0.0 ± 0.0
Asp
5.435AspAla: 5.435 ± 0.081
0.439AspCys: 0.439 ± 0.022
2.61AspAsp: 2.61 ± 0.062
3.444AspGlu: 3.444 ± 0.073
2.256AspPhe: 2.256 ± 0.055
4.117AspGly: 4.117 ± 0.082
1.212AspHis: 1.212 ± 0.04
3.91AspIle: 3.91 ± 0.075
2.322AspLys: 2.322 ± 0.063
4.972AspLeu: 4.972 ± 0.083
1.372AspMet: 1.372 ± 0.041
1.851AspAsn: 1.851 ± 0.047
2.441AspPro: 2.441 ± 0.049
1.949AspGln: 1.949 ± 0.045
2.775AspArg: 2.775 ± 0.063
2.64AspSer: 2.64 ± 0.053
2.555AspThr: 2.555 ± 0.065
3.671AspVal: 3.671 ± 0.074
0.881AspTrp: 0.881 ± 0.029
1.757AspTyr: 1.757 ± 0.052
0.0AspXaa: 0.0 ± 0.0
Glu
6.521GluAla: 6.521 ± 0.087
0.376GluCys: 0.376 ± 0.025
2.639GluAsp: 2.639 ± 0.065
3.46GluGlu: 3.46 ± 0.081
2.145GluPhe: 2.145 ± 0.053
3.572GluGly: 3.572 ± 0.069
1.654GluHis: 1.654 ± 0.048
3.852GluIle: 3.852 ± 0.074
2.89GluLys: 2.89 ± 0.062
6.261GluLeu: 6.261 ± 0.085
1.494GluMet: 1.494 ± 0.046
1.993GluAsn: 1.993 ± 0.048
2.243GluPro: 2.243 ± 0.058
3.253GluGln: 3.253 ± 0.065
3.897GluArg: 3.897 ± 0.08
3.098GluSer: 3.098 ± 0.059
2.79GluThr: 2.79 ± 0.072
4.242GluVal: 4.242 ± 0.081
0.75GluTrp: 0.75 ± 0.032
1.541GluTyr: 1.541 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.624PheAla: 3.624 ± 0.068
0.354PheCys: 0.354 ± 0.018
2.384PheAsp: 2.384 ± 0.05
2.089PheGlu: 2.089 ± 0.054
1.481PhePhe: 1.481 ± 0.048
3.113PheGly: 3.113 ± 0.07
0.812PheHis: 0.812 ± 0.032
2.249PheIle: 2.249 ± 0.054
1.497PheLys: 1.497 ± 0.04
3.343PheLeu: 3.343 ± 0.076
0.928PheMet: 0.928 ± 0.038
1.608PheAsn: 1.608 ± 0.04
1.54PhePro: 1.54 ± 0.041
1.143PheGln: 1.143 ± 0.039
1.896PheArg: 1.896 ± 0.05
2.894PheSer: 2.894 ± 0.066
2.062PheThr: 2.062 ± 0.062
2.444PheVal: 2.444 ± 0.059
0.499PheTrp: 0.499 ± 0.03
1.12PheTyr: 1.12 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
6.581GlyAla: 6.581 ± 0.11
0.739GlyCys: 0.739 ± 0.031
3.778GlyAsp: 3.778 ± 0.081
4.399GlyGlu: 4.399 ± 0.08
3.222GlyPhe: 3.222 ± 0.071
5.755GlyGly: 5.755 ± 0.16
1.85GlyHis: 1.85 ± 0.051
5.047GlyIle: 5.047 ± 0.074
4.1GlyLys: 4.1 ± 0.088
8.104GlyLeu: 8.104 ± 0.106
2.343GlyMet: 2.343 ± 0.052
2.993GlyAsn: 2.993 ± 0.108
2.134GlyPro: 2.134 ± 0.052
2.999GlyGln: 2.999 ± 0.062
4.366GlyArg: 4.366 ± 0.073
4.513GlySer: 4.513 ± 0.097
3.832GlyThr: 3.832 ± 0.096
5.436GlyVal: 5.436 ± 0.087
1.206GlyTrp: 1.206 ± 0.044
2.455GlyTyr: 2.455 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.667HisAla: 2.667 ± 0.063
0.249HisCys: 0.249 ± 0.017
1.423HisAsp: 1.423 ± 0.043
1.392HisGlu: 1.392 ± 0.045
1.055HisPhe: 1.055 ± 0.04
2.119HisGly: 2.119 ± 0.063
0.846HisHis: 0.846 ± 0.037
1.413HisIle: 1.413 ± 0.036
0.809HisLys: 0.809 ± 0.033
2.455HisLeu: 2.455 ± 0.053
0.599HisMet: 0.599 ± 0.028
0.694HisAsn: 0.694 ± 0.03
1.411HisPro: 1.411 ± 0.047
1.059HisGln: 1.059 ± 0.037
1.273HisArg: 1.273 ± 0.04
1.219HisSer: 1.219 ± 0.038
1.138HisThr: 1.138 ± 0.039
1.626HisVal: 1.626 ± 0.042
0.391HisTrp: 0.391 ± 0.019
0.906HisTyr: 0.906 ± 0.035
0.0HisXaa: 0.0 ± 0.0
Ile
6.779IleAla: 6.779 ± 0.103
0.488IleCys: 0.488 ± 0.029
3.54IleAsp: 3.54 ± 0.071
3.7IleGlu: 3.7 ± 0.066
1.921IlePhe: 1.921 ± 0.056
4.71IleGly: 4.71 ± 0.083
1.288IleHis: 1.288 ± 0.041
3.051IleIle: 3.051 ± 0.071
2.59IleLys: 2.59 ± 0.054
5.335IleLeu: 5.335 ± 0.087
1.292IleMet: 1.292 ± 0.043
2.26IleAsn: 2.26 ± 0.063
2.719IlePro: 2.719 ± 0.061
1.932IleGln: 1.932 ± 0.051
3.353IleArg: 3.353 ± 0.065
3.878IleSer: 3.878 ± 0.073
3.269IleThr: 3.269 ± 0.067
4.06IleVal: 4.06 ± 0.076
0.566IleTrp: 0.566 ± 0.026
1.477IleTyr: 1.477 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
4.296LysAla: 4.296 ± 0.073
0.204LysCys: 0.204 ± 0.016
1.989LysAsp: 1.989 ± 0.051
2.264LysGlu: 2.264 ± 0.062
1.248LysPhe: 1.248 ± 0.039
2.754LysGly: 2.754 ± 0.054
1.09LysHis: 1.09 ± 0.036
2.319LysIle: 2.319 ± 0.06
1.827LysLys: 1.827 ± 0.064
4.713LysLeu: 4.713 ± 0.084
0.999LysMet: 0.999 ± 0.035
1.416LysAsn: 1.416 ± 0.04
2.468LysPro: 2.468 ± 0.059
2.227LysGln: 2.227 ± 0.047
2.696LysArg: 2.696 ± 0.063
2.236LysSer: 2.236 ± 0.058
2.282LysThr: 2.282 ± 0.053
3.195LysVal: 3.195 ± 0.075
0.428LysTrp: 0.428 ± 0.025
0.967LysTyr: 0.967 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
12.101LeuAla: 12.101 ± 0.149
0.918LeuCys: 0.918 ± 0.034
6.065LeuAsp: 6.065 ± 0.097
6.279LeuGlu: 6.279 ± 0.091
3.786LeuPhe: 3.786 ± 0.079
8.008LeuGly: 8.008 ± 0.113
2.532LeuHis: 2.532 ± 0.059
5.445LeuIle: 5.445 ± 0.093
4.5LeuLys: 4.5 ± 0.077
11.759LeuLeu: 11.759 ± 0.188
2.607LeuMet: 2.607 ± 0.061
3.906LeuAsn: 3.906 ± 0.082
5.803LeuPro: 5.803 ± 0.089
4.911LeuGln: 4.911 ± 0.087
6.456LeuArg: 6.456 ± 0.101
6.871LeuSer: 6.871 ± 0.084
5.288LeuThr: 5.288 ± 0.07
7.3LeuVal: 7.3 ± 0.094
1.161LeuTrp: 1.161 ± 0.048
2.416LeuTyr: 2.416 ± 0.056
0.0LeuXaa: 0.0 ± 0.0
Met
2.795MetAla: 2.795 ± 0.06
0.143MetCys: 0.143 ± 0.014
1.222MetAsp: 1.222 ± 0.038
1.326MetGlu: 1.326 ± 0.037
0.8MetPhe: 0.8 ± 0.032
1.629MetGly: 1.629 ± 0.041
0.643MetHis: 0.643 ± 0.027
1.288MetIle: 1.288 ± 0.039
1.228MetLys: 1.228 ± 0.043
3.07MetLeu: 3.07 ± 0.064
0.749MetMet: 0.749 ± 0.03
0.888MetAsn: 0.888 ± 0.027
1.442MetPro: 1.442 ± 0.042
1.497MetGln: 1.497 ± 0.044
1.721MetArg: 1.721 ± 0.047
1.673MetSer: 1.673 ± 0.044
1.508MetThr: 1.508 ± 0.042
1.746MetVal: 1.746 ± 0.047
0.171MetTrp: 0.171 ± 0.015
0.42MetTyr: 0.42 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.664AsnAla: 3.664 ± 0.08
0.273AsnCys: 0.273 ± 0.021
1.647AsnAsp: 1.647 ± 0.054
1.686AsnGlu: 1.686 ± 0.044
1.24AsnPhe: 1.24 ± 0.044
2.906AsnGly: 2.906 ± 0.08
0.805AsnHis: 0.805 ± 0.028
2.385AsnIle: 2.385 ± 0.072
1.287AsnLys: 1.287 ± 0.04
3.645AsnLeu: 3.645 ± 0.074
0.857AsnMet: 0.857 ± 0.035
1.333AsnAsn: 1.333 ± 0.053
2.098AsnPro: 2.098 ± 0.054
1.462AsnGln: 1.462 ± 0.042
2.068AsnArg: 2.068 ± 0.05
1.85AsnSer: 1.85 ± 0.054
1.993AsnThr: 1.993 ± 0.064
2.443AsnVal: 2.443 ± 0.059
0.464AsnTrp: 0.464 ± 0.027
0.908AsnTyr: 0.908 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
4.966ProAla: 4.966 ± 0.082
0.346ProCys: 0.346 ± 0.019
2.953ProAsp: 2.953 ± 0.055
3.564ProGlu: 3.564 ± 0.075
1.681ProPhe: 1.681 ± 0.046
3.929ProGly: 3.929 ± 0.072
1.125ProHis: 1.125 ± 0.039
2.201ProIle: 2.201 ± 0.058
1.636ProLys: 1.636 ± 0.05
4.609ProLeu: 4.609 ± 0.096
1.075ProMet: 1.075 ± 0.04
1.464ProAsn: 1.464 ± 0.042
1.973ProPro: 1.973 ± 0.063
2.024ProGln: 2.024 ± 0.048
2.194ProArg: 2.194 ± 0.055
2.585ProSer: 2.585 ± 0.05
2.004ProThr: 2.004 ± 0.05
3.781ProVal: 3.781 ± 0.064
0.637ProTrp: 0.637 ± 0.027
1.275ProTyr: 1.275 ± 0.044
0.0ProXaa: 0.0 ± 0.0
Gln
5.646GlnAla: 5.646 ± 0.085
0.29GlnCys: 0.29 ± 0.019
2.205GlnAsp: 2.205 ± 0.054
2.547GlnGlu: 2.547 ± 0.068
1.471GlnPhe: 1.471 ± 0.044
3.211GlnGly: 3.211 ± 0.064
1.391GlnHis: 1.391 ± 0.044
2.098GlnIle: 2.098 ± 0.048
1.587GlnLys: 1.587 ± 0.044
4.638GlnLeu: 4.638 ± 0.086
1.029GlnMet: 1.029 ± 0.038
1.194GlnAsn: 1.194 ± 0.04
2.188GlnPro: 2.188 ± 0.056
2.704GlnGln: 2.704 ± 0.08
3.008GlnArg: 3.008 ± 0.068
2.356GlnSer: 2.356 ± 0.06
1.864GlnThr: 1.864 ± 0.045
3.274GlnVal: 3.274 ± 0.064
0.58GlnTrp: 0.58 ± 0.028
1.287GlnTyr: 1.287 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
5.045ArgAla: 5.045 ± 0.086
0.449ArgCys: 0.449 ± 0.024
3.146ArgAsp: 3.146 ± 0.068
3.868ArgGlu: 3.868 ± 0.075
2.507ArgPhe: 2.507 ± 0.049
3.569ArgGly: 3.569 ± 0.074
1.778ArgHis: 1.778 ± 0.052
3.818ArgIle: 3.818 ± 0.066
2.542ArgLys: 2.542 ± 0.063
6.902ArgLeu: 6.902 ± 0.099
1.609ArgMet: 1.609 ± 0.045
2.297ArgAsn: 2.297 ± 0.057
2.48ArgPro: 2.48 ± 0.051
3.154ArgGln: 3.154 ± 0.067
3.697ArgArg: 3.697 ± 0.077
2.972ArgSer: 2.972 ± 0.059
2.594ArgThr: 2.594 ± 0.059
3.879ArgVal: 3.879 ± 0.075
0.826ArgTrp: 0.826 ± 0.032
2.039ArgTyr: 2.039 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
5.79SerAla: 5.79 ± 0.104
0.508SerCys: 0.508 ± 0.027
2.749SerAsp: 2.749 ± 0.058
2.925SerGlu: 2.925 ± 0.062
2.253SerPhe: 2.253 ± 0.06
5.236SerGly: 5.236 ± 0.091
1.496SerHis: 1.496 ± 0.039
3.503SerIle: 3.503 ± 0.07
2.259SerLys: 2.259 ± 0.051
6.792SerLeu: 6.792 ± 0.098
1.602SerMet: 1.602 ± 0.044
2.023SerAsn: 2.023 ± 0.052
2.717SerPro: 2.717 ± 0.061
2.466SerGln: 2.466 ± 0.05
3.623SerArg: 3.623 ± 0.071
3.755SerSer: 3.755 ± 0.089
2.998SerThr: 2.998 ± 0.068
3.95SerVal: 3.95 ± 0.079
0.789SerTrp: 0.789 ± 0.031
1.607SerTyr: 1.607 ± 0.043
0.0SerXaa: 0.0 ± 0.0
Thr
4.875ThrAla: 4.875 ± 0.085
0.346ThrCys: 0.346 ± 0.023
2.509ThrAsp: 2.509 ± 0.055
2.564ThrGlu: 2.564 ± 0.059
1.848ThrPhe: 1.848 ± 0.048
4.423ThrGly: 4.423 ± 0.1
1.136ThrHis: 1.136 ± 0.039
2.929ThrIle: 2.929 ± 0.069
1.639ThrLys: 1.639 ± 0.046
6.17ThrLeu: 6.17 ± 0.097
1.189ThrMet: 1.189 ± 0.039
1.534ThrAsn: 1.534 ± 0.051
2.969ThrPro: 2.969 ± 0.062
2.015ThrGln: 2.015 ± 0.058
2.744ThrArg: 2.744 ± 0.067
2.922ThrSer: 2.922 ± 0.062
2.646ThrThr: 2.646 ± 0.067
3.664ThrVal: 3.664 ± 0.07
0.623ThrTrp: 0.623 ± 0.024
1.285ThrTyr: 1.285 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
7.425ValAla: 7.425 ± 0.097
0.566ValCys: 0.566 ± 0.025
3.926ValAsp: 3.926 ± 0.071
4.259ValGlu: 4.259 ± 0.085
2.638ValPhe: 2.638 ± 0.071
4.756ValGly: 4.756 ± 0.086
1.439ValHis: 1.439 ± 0.04
4.2ValIle: 4.2 ± 0.073
2.992ValLys: 2.992 ± 0.067
7.549ValLeu: 7.549 ± 0.105
1.927ValMet: 1.927 ± 0.056
2.61ValAsn: 2.61 ± 0.062
3.155ValPro: 3.155 ± 0.064
2.695ValGln: 2.695 ± 0.055
3.922ValArg: 3.922 ± 0.071
4.467ValSer: 4.467 ± 0.076
4.019ValThr: 4.019 ± 0.084
5.421ValVal: 5.421 ± 0.093
0.742ValTrp: 0.742 ± 0.03
1.728ValTyr: 1.728 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
0.954TrpAla: 0.954 ± 0.036
0.106TrpCys: 0.106 ± 0.012
0.56TrpAsp: 0.56 ± 0.028
0.599TrpGlu: 0.599 ± 0.025
0.475TrpPhe: 0.475 ± 0.025
0.782TrpGly: 0.782 ± 0.028
0.428TrpHis: 0.428 ± 0.024
0.714TrpIle: 0.714 ± 0.026
0.527TrpLys: 0.527 ± 0.027
1.948TrpLeu: 1.948 ± 0.057
0.376TrpMet: 0.376 ± 0.021
0.441TrpAsn: 0.441 ± 0.027
0.475TrpPro: 0.475 ± 0.023
0.963TrpGln: 0.963 ± 0.036
0.912TrpArg: 0.912 ± 0.039
0.693TrpSer: 0.693 ± 0.027
0.534TrpThr: 0.534 ± 0.027
0.861TrpVal: 0.861 ± 0.035
0.209TrpTrp: 0.209 ± 0.018
0.322TrpTyr: 0.322 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.769TyrAla: 2.769 ± 0.056
0.283TyrCys: 0.283 ± 0.019
1.475TyrAsp: 1.475 ± 0.046
1.404TyrGlu: 1.404 ± 0.044
1.154TyrPhe: 1.154 ± 0.042
2.146TyrGly: 2.146 ± 0.053
0.696TyrHis: 0.696 ± 0.03
1.353TyrIle: 1.353 ± 0.041
0.997TyrLys: 0.997 ± 0.039
2.913TyrLeu: 2.913 ± 0.062
0.602TyrMet: 0.602 ± 0.028
0.872TyrAsn: 0.872 ± 0.034
1.345TyrPro: 1.345 ± 0.04
1.384TyrGln: 1.384 ± 0.041
1.918TyrArg: 1.918 ± 0.046
1.633TyrSer: 1.633 ± 0.044
1.379TyrThr: 1.379 ± 0.037
1.735TyrVal: 1.735 ± 0.044
0.425TyrTrp: 0.425 ± 0.024
0.855TyrTyr: 0.855 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2609 proteins (847630 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski