Vibrio virus CTXphi
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7BBA4|Q7BBA4_9VIRU Cep OS=Vibrio virus CTXphi OX=141904 GN=cep PE=4 SV=1
MM1 pKa = 9.05 LMMDD5 pKa = 4.67 TLYY8 pKa = 11.2 DD9 pKa = 3.44 WLIDD13 pKa = 3.64 GFTWLVIKK21 pKa = 10.51 LGIMWIEE28 pKa = 3.84 SKK30 pKa = 10.71 IFVIQFFWEE39 pKa = 4.3 MSQKK43 pKa = 10.43 VIDD46 pKa = 4.21 MFTIYY51 pKa = 10.39 PLIQQAIDD59 pKa = 3.75 MLPPQYY65 pKa = 10.95 SGFLFFLGLDD75 pKa = 3.32 QALAIVLQALMTRR88 pKa = 11.84 FALRR92 pKa = 11.84 ALNLL96 pKa = 3.56
Molecular weight: 11.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.47
IPC2_protein 4.457
IPC_protein 4.253
Toseland 4.037
ProMoST 4.431
Dawson 4.253
Bjellqvist 4.406
Wikipedia 4.215
Rodwell 4.075
Grimsley 3.961
Solomon 4.228
Lehninger 4.19
Nozaki 4.38
DTASelect 4.635
Thurlkill 4.113
EMBOSS 4.215
Sillero 4.368
Patrickios 3.897
IPC_peptide 4.228
IPC2_peptide 4.342
IPC2.peptide.svr19 4.33
Protein with the highest isoelectric point:
>tr|Q8LT24|Q8LT24_9VIRU Cholera toxin B subunit OS=Vibrio virus CTXphi OX=141904 GN=ctxB PE=4 SV=1
MM1 pKa = 7.64 FSSLKK6 pKa = 10.78 NKK8 pKa = 10.41 LNTFKK13 pKa = 10.35 STLSLGVFLLFSAFANQALAAADD36 pKa = 3.71 AGLVTEE42 pKa = 4.41 VTKK45 pKa = 10.35 TLGTSKK51 pKa = 10.11 DD52 pKa = 3.48 TVIALGPLIMGVVGAIVLIVTVIGLIRR79 pKa = 11.84 KK80 pKa = 8.93 AKK82 pKa = 9.89
Molecular weight: 8.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.985
IPC2_protein 9.341
IPC_protein 9.282
Toseland 10.57
ProMoST 10.043
Dawson 10.613
Bjellqvist 10.116
Wikipedia 10.657
Rodwell 11.506
Grimsley 10.613
Solomon 10.643
Lehninger 10.643
Nozaki 10.511
DTASelect 10.131
Thurlkill 10.511
EMBOSS 10.921
Sillero 10.511
Patrickios 11.301
IPC_peptide 10.657
IPC2_peptide 8.361
IPC2.peptide.svr19 8.32
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
1949
82
399
216.6
24.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.978 ± 0.617
1.437 ± 0.364
5.849 ± 0.353
5.233 ± 0.704
5.49 ± 0.429
6.26 ± 0.547
2.668 ± 0.535
6.106 ± 0.476
5.798 ± 0.762
9.8 ± 0.751
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.873 ± 0.344
4.361 ± 0.468
4.72 ± 0.514
3.489 ± 0.606
4.926 ± 0.568
7.542 ± 0.719
5.182 ± 0.702
6.106 ± 0.586
1.539 ± 0.322
3.643 ± 0.542
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here