Banna-like virus strain Balaton/2010/HUN

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Seadornavirus; unclassified Seadornavirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9Z350|M9Z350_9REOV VP9 OS=Banna-like virus strain Balaton/2010/HUN OX=1325460 PE=4 SV=1
MM1 pKa = 7.58SGTKK5 pKa = 10.11RR6 pKa = 11.84IQLRR10 pKa = 11.84NGQGSIIKK18 pKa = 8.95TGQSYY23 pKa = 8.3EE24 pKa = 4.04LRR26 pKa = 11.84CRR28 pKa = 11.84RR29 pKa = 11.84LDD31 pKa = 3.46IDD33 pKa = 4.04CTLPLPGVTDD43 pKa = 3.26SRR45 pKa = 11.84GITDD49 pKa = 4.01SIKK52 pKa = 11.1YY53 pKa = 8.61IVEE56 pKa = 4.16YY57 pKa = 10.89LGLTYY62 pKa = 10.58VDD64 pKa = 4.15SNNGIGVDD72 pKa = 3.54EE73 pKa = 4.95RR74 pKa = 11.84DD75 pKa = 3.77FEE77 pKa = 4.68HH78 pKa = 6.8FQANPTLLVCRR89 pKa = 11.84TSIGPIFLKK98 pKa = 10.46KK99 pKa = 10.21LRR101 pKa = 11.84SLPVCKK107 pKa = 10.17CAFFEE112 pKa = 4.39SKK114 pKa = 11.19YY115 pKa = 10.93NILVGLKK122 pKa = 10.3GVIRR126 pKa = 11.84LRR128 pKa = 11.84NDD130 pKa = 2.95RR131 pKa = 11.84TGYY134 pKa = 9.82RR135 pKa = 11.84YY136 pKa = 10.06GVIMNQCFPPVINFTNFVEE155 pKa = 4.85AVCNLNYY162 pKa = 10.4LHH164 pKa = 6.95RR165 pKa = 11.84SEE167 pKa = 4.8VAVLHH172 pKa = 6.7GDD174 pKa = 3.73VNPGNIMSDD183 pKa = 3.25DD184 pKa = 3.95RR185 pKa = 11.84GCLKK189 pKa = 10.67LVDD192 pKa = 4.3PVCILEE198 pKa = 4.36GAVNMVNADD207 pKa = 3.74YY208 pKa = 11.45DD209 pKa = 4.31DD210 pKa = 4.19LTQACEE216 pKa = 3.87MKK218 pKa = 10.77VFIHH222 pKa = 6.33SLVILLAKK230 pKa = 9.77QFKK233 pKa = 9.85TSISEE238 pKa = 4.0LRR240 pKa = 11.84IDD242 pKa = 3.88YY243 pKa = 11.29SNVNPVFSTTKK254 pKa = 10.21GSPLEE259 pKa = 4.6SILDD263 pKa = 3.6YY264 pKa = 11.19SADD267 pKa = 5.41DD268 pKa = 4.1IISWKK273 pKa = 7.98RR274 pKa = 11.84TIMEE278 pKa = 4.38APALPTKK285 pKa = 10.9VNVFNDD291 pKa = 3.5EE292 pKa = 4.22YY293 pKa = 11.43YY294 pKa = 10.81RR295 pKa = 11.84LADD298 pKa = 3.72IPTMITEE305 pKa = 4.38NLDD308 pKa = 3.66DD309 pKa = 5.95DD310 pKa = 6.09DD311 pKa = 4.28YY312 pKa = 11.76TT313 pKa = 4.05

Molecular weight: 35.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9Z5E3|M9Z5E3_9REOV VP6 OS=Banna-like virus strain Balaton/2010/HUN OX=1325460 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 4.52IVRR5 pKa = 11.84TKK7 pKa = 10.4SQSAAITVGISQFFDD22 pKa = 3.22LTQNRR27 pKa = 11.84AVIEE31 pKa = 4.46TYY33 pKa = 10.75SEE35 pKa = 3.96FRR37 pKa = 11.84KK38 pKa = 10.33RR39 pKa = 11.84NAFILKK45 pKa = 9.73YY46 pKa = 10.48AEE48 pKa = 3.9QLFTGLPSLFKK59 pKa = 11.14DD60 pKa = 3.59EE61 pKa = 5.67LDD63 pKa = 3.52GLKK66 pKa = 10.31QIIEE70 pKa = 4.4SEE72 pKa = 4.03GGANSFAKK80 pKa = 8.82ITQVGLSHH88 pKa = 6.13MTQFLLSAKK97 pKa = 10.21LPADD101 pKa = 4.34DD102 pKa = 4.2YY103 pKa = 11.36LARR106 pKa = 11.84ALRR109 pKa = 11.84FIVFQIHH116 pKa = 6.03SNEE119 pKa = 3.75LRR121 pKa = 11.84KK122 pKa = 10.46GGALLMFDD130 pKa = 4.64NLLAYY135 pKa = 10.0LKK137 pKa = 10.55KK138 pKa = 10.04HH139 pKa = 5.4EE140 pKa = 4.31RR141 pKa = 11.84TNKK144 pKa = 9.42QSLISYY150 pKa = 6.79VTKK153 pKa = 10.75DD154 pKa = 3.66DD155 pKa = 3.31VDD157 pKa = 4.32IIIVNGKK164 pKa = 6.76TLTYY168 pKa = 10.11VCRR171 pKa = 11.84NADD174 pKa = 3.36ALKK177 pKa = 9.59TKK179 pKa = 10.01EE180 pKa = 3.76YY181 pKa = 11.06ANAEE185 pKa = 4.0GHH187 pKa = 6.38FVITDD192 pKa = 4.17TIGSFRR198 pKa = 11.84ASSSGMDD205 pKa = 2.95AVTFMTLKK213 pKa = 10.67SEE215 pKa = 4.23AEE217 pKa = 4.1HH218 pKa = 6.96IINLFGNAISIGQLYY233 pKa = 9.53YY234 pKa = 10.38RR235 pKa = 11.84QLHH238 pKa = 6.21AEE240 pKa = 4.07VSTLAKK246 pKa = 10.49LKK248 pKa = 10.32ANPGKK253 pKa = 8.19VHH255 pKa = 6.91RR256 pKa = 11.84MHH258 pKa = 6.94GNVRR262 pKa = 11.84DD263 pKa = 3.86GFVRR267 pKa = 11.84RR268 pKa = 11.84LNDD271 pKa = 3.43STKK274 pKa = 10.48HH275 pKa = 5.33RR276 pKa = 11.84VTGAQRR282 pKa = 11.84ALDD285 pKa = 3.61HH286 pKa = 6.94SNIIRR291 pKa = 11.84FKK293 pKa = 11.06SSGAGKK299 pKa = 9.71DD300 pKa = 3.23EE301 pKa = 4.26RR302 pKa = 11.84LTYY305 pKa = 10.75LVDD308 pKa = 3.4EE309 pKa = 5.21CFLKK313 pKa = 11.19SMLPDD318 pKa = 3.12NRR320 pKa = 11.84ACARR324 pKa = 11.84IDD326 pKa = 3.92NINALIASKK335 pKa = 10.5LLHH338 pKa = 6.66DD339 pKa = 4.71CKK341 pKa = 10.68IGTACMHH348 pKa = 6.66CGVIGSMDD356 pKa = 3.58TVSQLSEE363 pKa = 4.13RR364 pKa = 11.84GVDD367 pKa = 3.38IVANTNEE374 pKa = 4.49TLDD377 pKa = 3.78AMAAPVMTKK386 pKa = 10.31PEE388 pKa = 4.35AEE390 pKa = 4.38TVTDD394 pKa = 3.61ASLNARR400 pKa = 11.84IVQSKK405 pKa = 8.34GQSYY409 pKa = 9.54VPKK412 pKa = 10.06TEE414 pKa = 3.97YY415 pKa = 9.99KK416 pKa = 10.36AKK418 pKa = 10.24KK419 pKa = 9.93SEE421 pKa = 4.06PADD424 pKa = 3.12SGFTVKK430 pKa = 10.45VEE432 pKa = 4.51SNHH435 pKa = 5.93SVTDD439 pKa = 3.76ALVAGVVSRR448 pKa = 11.84EE449 pKa = 3.83KK450 pKa = 11.36LNFF453 pKa = 3.58

Molecular weight: 50.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5704

182

1214

518.5

57.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.679 ± 0.623

1.192 ± 0.195

6.189 ± 0.289

4.786 ± 0.247

3.629 ± 0.222

5.575 ± 0.256

2.454 ± 0.286

6.697 ± 0.236

4.488 ± 0.357

9.309 ± 0.316

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.152

5.54 ± 0.343

4.418 ± 0.32

3.384 ± 0.298

5.75 ± 0.329

7.626 ± 0.171

7.048 ± 0.408

6.96 ± 0.282

0.666 ± 0.143

3.839 ± 0.199
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski