Cryptosporangium aurantiacum
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8789 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M7NP67|A0A1M7NP67_9ACTN Uncharacterized protein OS=Cryptosporangium aurantiacum OX=134849 GN=SAMN05443668_102748 PE=4 SV=1
MM1 pKa = 7.95 ADD3 pKa = 5.07 FEE5 pKa = 5.3 DD6 pKa = 3.88 QVDD9 pKa = 3.62 WDD11 pKa = 4.91 EE12 pKa = 4.06 IDD14 pKa = 3.33 YY15 pKa = 8.27 WTDD18 pKa = 2.71 RR19 pKa = 11.84 CGACDD24 pKa = 3.74 EE25 pKa = 4.69 RR26 pKa = 11.84 CGGTCEE32 pKa = 5.34 DD33 pKa = 4.44 YY34 pKa = 11.17 PIDD37 pKa = 3.75 TTKK40 pKa = 10.74 EE41 pKa = 4.09 GPRR44 pKa = 3.52
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.63
Rodwell 3.49
Grimsley 3.363
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.024
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.783
Patrickios 0.693
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.728
Protein with the highest isoelectric point:
>tr|A0A1M7RDX3|A0A1M7RDX3_9ACTN VOC domain-containing protein OS=Cryptosporangium aurantiacum OX=134849 GN=SAMN05443668_110200 PE=4 SV=1
MM1 pKa = 8.19 DD2 pKa = 5.11 IGHH5 pKa = 6.86 TPLQCSGQMLGGSNPCKK22 pKa = 10.16 APARR26 pKa = 11.84 ATVRR30 pKa = 11.84 PTVVPVPGRR39 pKa = 11.84 TPRR42 pKa = 11.84 CPASRR47 pKa = 11.84 PRR49 pKa = 11.84 SDD51 pKa = 3.38 GQSRR55 pKa = 11.84 RR56 pKa = 11.84 HH57 pKa = 5.54 PGPRR61 pKa = 11.84 RR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 PTVCRR69 pKa = 11.84 RR70 pKa = 11.84 PSTCSPVPDD79 pKa = 3.88 GRR81 pKa = 11.84 RR82 pKa = 11.84 APPARR87 pKa = 11.84 TGSSGSPRR95 pKa = 11.84 RR96 pKa = 11.84 PRR98 pKa = 11.84 CPRR101 pKa = 11.84 SRR103 pKa = 11.84 ARR105 pKa = 11.84 TPRR108 pKa = 11.84 PPGRR112 pKa = 11.84 RR113 pKa = 11.84 RR114 pKa = 11.84 RR115 pKa = 11.84 ASSRR119 pKa = 11.84 RR120 pKa = 11.84 CRR122 pKa = 11.84 SGRR125 pKa = 11.84 GPTAPSRR132 pKa = 11.84 RR133 pKa = 11.84 HH134 pKa = 5.37 RR135 pKa = 11.84 RR136 pKa = 11.84 TPGRR140 pKa = 11.84 TPRR143 pKa = 11.84 PCPAWHH149 pKa = 6.25 RR150 pKa = 11.84 TSSGSRR156 pKa = 11.84 RR157 pKa = 11.84 RR158 pKa = 11.84 RR159 pKa = 11.84 RR160 pKa = 11.84 VGRR163 pKa = 11.84 VRR165 pKa = 11.84 WVPVTLTRR173 pKa = 11.84 RR174 pKa = 11.84 TSSGSASSAIRR185 pKa = 11.84 RR186 pKa = 11.84 PRR188 pKa = 11.84 TGRR191 pKa = 11.84 RR192 pKa = 11.84 RR193 pKa = 11.84 QRR195 pKa = 11.84 PVPSPSNSRR204 pKa = 11.84 RR205 pKa = 11.84 RR206 pKa = 11.84 SRR208 pKa = 11.84 SPVANHH214 pKa = 6.93 RR215 pKa = 11.84 GRR217 pKa = 11.84 ARR219 pKa = 11.84 SSRR222 pKa = 11.84 PAANRR227 pKa = 11.84 SGQAQSSRR235 pKa = 11.84 PAANRR240 pKa = 11.84 SGRR243 pKa = 11.84 ARR245 pKa = 11.84 SSRR248 pKa = 11.84 PVASRR253 pKa = 11.84 RR254 pKa = 11.84 GRR256 pKa = 11.84 ARR258 pKa = 11.84 SSTRR262 pKa = 11.84 RR263 pKa = 11.84 RR264 pKa = 11.84 ARR266 pKa = 11.84 SSSGRR271 pKa = 11.84 GSTSRR276 pKa = 11.84 RR277 pKa = 11.84 GSTSRR282 pKa = 11.84 RR283 pKa = 11.84 GSTSRR288 pKa = 11.84 RR289 pKa = 11.84 GSTSGPRR296 pKa = 11.84 SRR298 pKa = 11.84 RR299 pKa = 11.84 ASSGRR304 pKa = 11.84 RR305 pKa = 11.84 SSHH308 pKa = 6.07 RR309 pKa = 11.84 ASSSGRR315 pKa = 11.84 RR316 pKa = 11.84 SNRR319 pKa = 11.84 TNNSNGPPSSRR330 pKa = 11.84 RR331 pKa = 11.84 ASSSGRR337 pKa = 11.84 RR338 pKa = 11.84 RR339 pKa = 11.84 HH340 pKa = 5.39 RR341 pKa = 11.84 TTSSSGRR348 pKa = 11.84 RR349 pKa = 11.84 SSNRR353 pKa = 11.84 RR354 pKa = 11.84 SGRR357 pKa = 11.84 SSSPAGRR364 pKa = 11.84 RR365 pKa = 11.84 RR366 pKa = 11.84 NRR368 pKa = 11.84 SGPRR372 pKa = 11.84 NSSGPARR379 pKa = 11.84 RR380 pKa = 11.84 TSGLPSRR387 pKa = 11.84 SGPRR391 pKa = 11.84 SSSGRR396 pKa = 11.84 GRR398 pKa = 11.84 RR399 pKa = 11.84 TSGPRR404 pKa = 11.84 RR405 pKa = 11.84 SSSNGLRR412 pKa = 11.84 SSHH415 pKa = 6.46 SGPPSSSPRR424 pKa = 11.84 RR425 pKa = 11.84 RR426 pKa = 11.84 PTSRR430 pKa = 11.84 PSPSSPTSPSNGRR443 pKa = 11.84 RR444 pKa = 11.84 RR445 pKa = 11.84 SSSGPHH451 pKa = 5.0 PRR453 pKa = 11.84 IRR455 pKa = 11.84 TTRR458 pKa = 11.84 SPGSRR463 pKa = 11.84 PPSRR467 pKa = 11.84 AGSRR471 pKa = 11.84 SPATPRR477 pKa = 11.84 PPRR480 pKa = 11.84 RR481 pKa = 11.84 RR482 pKa = 11.84 RR483 pKa = 11.84 DD484 pKa = 3.36 SRR486 pKa = 11.84 SPASPCTTTRR496 pKa = 11.84 YY497 pKa = 9.45 PGSPSPGSPSPGSPSPGSPSPGSPSPGSRR526 pKa = 11.84 CTTTRR531 pKa = 11.84 YY532 pKa = 9.41 PGSRR536 pKa = 11.84 PTGSPSRR543 pKa = 11.84 GSPSRR548 pKa = 11.84 GSTLSPTRR556 pKa = 3.68
Molecular weight: 59.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.14
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.364
Rodwell 12.384
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.076
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.181
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8789
0
8789
2911538
24
8163
331.3
35.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.174 ± 0.044
0.695 ± 0.007
6.157 ± 0.02
5.265 ± 0.024
2.749 ± 0.016
9.185 ± 0.027
2.032 ± 0.012
3.41 ± 0.018
1.652 ± 0.018
10.575 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.513 ± 0.009
1.728 ± 0.014
6.241 ± 0.024
2.585 ± 0.015
8.131 ± 0.03
5.076 ± 0.017
6.151 ± 0.022
9.129 ± 0.028
1.539 ± 0.01
2.013 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here