Pseudomonas caspiana

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas syringae group

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5280 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3P468|A0A1Y3P468_9PSED Uncharacterized protein OS=Pseudomonas caspiana OX=1451454 GN=AUC60_07720 PE=4 SV=1
MM1 pKa = 7.43VGFIGTVRR9 pKa = 11.84QVVGEE14 pKa = 4.12VFAVAGDD21 pKa = 3.57GTRR24 pKa = 11.84RR25 pKa = 11.84LLIEE29 pKa = 4.32GDD31 pKa = 3.35RR32 pKa = 11.84LFAGEE37 pKa = 3.93QLQTGSGGAVAVNLDD52 pKa = 3.37AGGEE56 pKa = 4.15LTLGRR61 pKa = 11.84GSSLQLTPQLLNHH74 pKa = 6.01QAGHH78 pKa = 5.01VTTGDD83 pKa = 3.47EE84 pKa = 4.49LTPSLTPSSSTPAVLPEE101 pKa = 4.01QVFSADD107 pKa = 4.22APTQTPEE114 pKa = 3.6AAAPEE119 pKa = 4.24PAPDD123 pKa = 4.03GSSLGGGHH131 pKa = 6.44SAMLLTEE138 pKa = 4.12VGGEE142 pKa = 3.94VSPEE146 pKa = 3.54IGFTTQGLSSAPEE159 pKa = 3.87FPEE162 pKa = 4.16GRR164 pKa = 11.84IEE166 pKa = 4.2GLPDD170 pKa = 3.34SSDD173 pKa = 3.12ASGSVFVPPVVVVPPTEE190 pKa = 4.13PEE192 pKa = 4.06PPVEE196 pKa = 4.03PEE198 pKa = 4.15PPVEE202 pKa = 4.22HH203 pKa = 6.71PVDD206 pKa = 3.63HH207 pKa = 6.44GVEE210 pKa = 3.95LSGGQLTLAEE220 pKa = 5.17GDD222 pKa = 4.14LSCGPVTQSGVFTVNAVDD240 pKa = 4.25GLQSLVVGGLAVISGGVVAGFPLSTQTPLGNTFSVTAYY278 pKa = 10.47DD279 pKa = 3.72PATGEE284 pKa = 3.83VSYY287 pKa = 11.07SYY289 pKa = 9.63TLQGAIEE296 pKa = 4.29HH297 pKa = 6.52PVGEE301 pKa = 4.5GANTLGEE308 pKa = 4.42SFTVVARR315 pKa = 11.84DD316 pKa = 3.52TDD318 pKa = 3.73GDD320 pKa = 4.0QADD323 pKa = 3.76STLDD327 pKa = 3.25INIVDD332 pKa = 4.72DD333 pKa = 4.24VPEE336 pKa = 3.87AHH338 pKa = 7.04CIEE341 pKa = 4.47VTLSADD347 pKa = 3.81PDD349 pKa = 4.17CPGAPLPGASGSLLQGGTFGADD371 pKa = 2.48GGFIHH376 pKa = 7.13SISLNGTTYY385 pKa = 10.73SYY387 pKa = 11.4DD388 pKa = 3.59PEE390 pKa = 4.77TGCSTGSCEE399 pKa = 4.16GTGVFDD405 pKa = 4.04PATYY409 pKa = 10.71SLTVTSEE416 pKa = 3.97GGGTLVVNMLNGDD429 pKa = 4.24FSYY432 pKa = 10.84TPSTNPACEE441 pKa = 3.83PLTEE445 pKa = 4.6NIHH448 pKa = 6.05YY449 pKa = 9.96VISDD453 pKa = 3.56NDD455 pKa = 3.49GDD457 pKa = 4.35LAGSDD462 pKa = 3.87LQIHH466 pKa = 6.0VPAILPPEE474 pKa = 4.68GPTAVVDD481 pKa = 3.86NVITNILAPCIEE493 pKa = 4.46VPGVLLVTNDD503 pKa = 3.32VPGSGGPLTASPTVFHH519 pKa = 6.65TGWQVKK525 pKa = 10.63GEE527 pKa = 4.22GFSNDD532 pKa = 3.29CQKK535 pKa = 10.0TIDD538 pKa = 3.96FSGKK542 pKa = 9.3RR543 pKa = 11.84DD544 pKa = 3.42NSANQLKK551 pKa = 10.2NLEE554 pKa = 4.27RR555 pKa = 11.84SDD557 pKa = 3.98FANTGAMTALVVITGYY573 pKa = 10.9LGAFSGPGANAQDD586 pKa = 4.31LYY588 pKa = 11.35SVNLMAGEE596 pKa = 4.32SVAVDD601 pKa = 4.23LKK603 pKa = 11.49SLGDD607 pKa = 3.49QVGVFWQLDD616 pKa = 3.46GGEE619 pKa = 4.41FQPLDD624 pKa = 3.45AGGTFTATEE633 pKa = 3.89NGVYY637 pKa = 10.3RR638 pKa = 11.84LLVVNQPDD646 pKa = 3.45AGQPGPAVHH655 pKa = 5.73YY656 pKa = 9.45TLDD659 pKa = 3.7LVIDD663 pKa = 3.9YY664 pKa = 10.28SAADD668 pKa = 3.49TTPNHH673 pKa = 6.07EE674 pKa = 4.1GAYY677 pKa = 8.45TVNDD681 pKa = 3.52GHH683 pKa = 6.62GGSDD687 pKa = 3.09SAAVNISYY695 pKa = 10.58QEE697 pKa = 4.24GCTLLGTTGDD707 pKa = 3.95DD708 pKa = 3.55VLLGGAQDD716 pKa = 4.41DD717 pKa = 4.51SLHH720 pKa = 6.65GGDD723 pKa = 4.84GDD725 pKa = 4.46DD726 pKa = 4.09VLSGGAGNNDD736 pKa = 3.21LYY738 pKa = 11.37GDD740 pKa = 3.93NGNDD744 pKa = 3.37VLFSGSGNDD753 pKa = 3.67VLDD756 pKa = 4.39GGAGNNTASYY766 pKa = 10.87ALADD770 pKa = 3.6SGVTVSTAQLGPQHH784 pKa = 6.5TGGAGTDD791 pKa = 3.39TLINIQNLTGSDD803 pKa = 3.95YY804 pKa = 11.33DD805 pKa = 3.61DD806 pKa = 5.18HH807 pKa = 6.88LTGDD811 pKa = 3.74YY812 pKa = 8.44EE813 pKa = 5.1ANVINGGLGNDD824 pKa = 3.62VLVGGLGNDD833 pKa = 3.71TLTGGAGHH841 pKa = 7.22DD842 pKa = 3.69TFKK845 pKa = 10.78WLAGDD850 pKa = 3.84SGHH853 pKa = 7.49DD854 pKa = 3.6LVTDD858 pKa = 4.35FSLGADD864 pKa = 3.41TLDD867 pKa = 4.64LSKK870 pKa = 10.89LLQGFSVGTDD880 pKa = 3.14SLDD883 pKa = 3.66DD884 pKa = 3.86FLHH887 pKa = 6.36FKK889 pKa = 9.13VTGSGADD896 pKa = 3.33LVSSIEE902 pKa = 3.98IGSGGSSQTIDD913 pKa = 3.25LAGVNLAQHH922 pKa = 6.34YY923 pKa = 9.9DD924 pKa = 3.5VGVGAGGIVAGADD937 pKa = 3.02AGSIISGMLGDD948 pKa = 3.91HH949 pKa = 6.4SLRR952 pKa = 11.84ADD954 pKa = 3.56VVV956 pKa = 3.47

Molecular weight: 96.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3P3S5|A0A1Y3P3S5_9PSED TetR family transcriptional regulator OS=Pseudomonas caspiana OX=1451454 GN=AUC60_23615 PE=4 SV=1
MM1 pKa = 8.22DD2 pKa = 5.9DD3 pKa = 4.11ALIAALLTQVTFWLCMSLFIRR24 pKa = 11.84LFTFRR29 pKa = 11.84RR30 pKa = 11.84KK31 pKa = 9.24GARR34 pKa = 11.84FRR36 pKa = 11.84RR37 pKa = 11.84DD38 pKa = 3.31MSCLAWLMMASAGAAMVYY56 pKa = 9.64IGKK59 pKa = 10.33GMLIVQVTAWPLVLILAVFVGSVYY83 pKa = 10.45QSSGNLARR91 pKa = 11.84VWKK94 pKa = 10.63VGG96 pKa = 3.19

Molecular weight: 10.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5280

0

5280

1716129

36

5915

325.0

35.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.631 ± 0.038

0.937 ± 0.011

5.411 ± 0.026

5.702 ± 0.026

3.707 ± 0.021

7.74 ± 0.034

2.183 ± 0.016

5.114 ± 0.028

3.757 ± 0.031

11.471 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.017

3.219 ± 0.023

4.764 ± 0.024

4.466 ± 0.027

6.11 ± 0.029

6.185 ± 0.025

5.058 ± 0.025

7.178 ± 0.027

1.407 ± 0.015

2.54 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski