Aconitum latent virus
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91UJ1|Q91UJ1_9VIRU Movement protein TGB2 OS=Aconitum latent virus OX=101764 GN=ORF3 PE=3 SV=1
MM1 pKa = 7.31 NLVLICGLLSFAIALYY17 pKa = 10.06 FLSINDD23 pKa = 4.01 RR24 pKa = 11.84 GDD26 pKa = 3.39 CFVIITGEE34 pKa = 4.05 SIRR37 pKa = 11.84 IQGCHH42 pKa = 5.74 INRR45 pKa = 11.84 EE46 pKa = 4.24 FNEE49 pKa = 4.12 GLKK52 pKa = 10.62 GLRR55 pKa = 11.84 ALNNEE60 pKa = 4.44 CLL62 pKa = 3.7
Molecular weight: 6.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.288
IPC2_protein 5.639
IPC_protein 5.423
Toseland 5.474
ProMoST 5.652
Dawson 5.537
Bjellqvist 5.601
Wikipedia 5.474
Rodwell 5.499
Grimsley 5.448
Solomon 5.537
Lehninger 5.512
Nozaki 5.728
DTASelect 5.855
Thurlkill 5.741
EMBOSS 5.69
Sillero 5.817
Patrickios 3.427
IPC_peptide 5.55
IPC2_peptide 5.83
IPC2.peptide.svr19 5.939
Protein with the highest isoelectric point:
>tr|Q91UI9|Q91UI9_9VIRU Capsid protein OS=Aconitum latent virus OX=101764 GN=ORF5 PE=3 SV=1
MM1 pKa = 7.07 KK2 pKa = 9.36 WWRR5 pKa = 11.84 EE6 pKa = 3.89 VALLLYY12 pKa = 10.69 ADD14 pKa = 5.98 FINKK18 pKa = 9.65 CGRR21 pKa = 11.84 AEE23 pKa = 3.86 FALCVDD29 pKa = 4.0 IALASARR36 pKa = 11.84 PVGGGTSSYY45 pKa = 10.5 ARR47 pKa = 11.84 KK48 pKa = 9.62 RR49 pKa = 11.84 RR50 pKa = 11.84 AKK52 pKa = 10.98 AMGRR56 pKa = 11.84 CHH58 pKa = 6.61 RR59 pKa = 11.84 CFRR62 pKa = 11.84 VWPPTYY68 pKa = 9.09 FTKK71 pKa = 10.62 RR72 pKa = 11.84 CDD74 pKa = 4.51 GINCRR79 pKa = 11.84 PGISHH84 pKa = 6.58 NLSVEE89 pKa = 3.8 RR90 pKa = 11.84 RR91 pKa = 11.84 IKK93 pKa = 10.85 LGVTAVIPSRR103 pKa = 11.84 PNN105 pKa = 2.87
Molecular weight: 11.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.633
IPC_protein 10.409
Toseland 10.643
ProMoST 10.35
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.95
Rodwell 10.95
Grimsley 10.789
Solomon 10.862
Lehninger 10.833
Nozaki 10.687
DTASelect 10.452
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.701
IPC_peptide 10.877
IPC2_peptide 9.853
IPC2.peptide.svr19 8.484
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2795
62
1986
465.8
52.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.37 ± 0.759
2.97 ± 0.427
5.116 ± 0.48
6.44 ± 0.563
4.973 ± 0.553
6.369 ± 0.385
2.54 ± 0.218
5.259 ± 0.542
5.51 ± 0.43
10.304 ± 0.731
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.004 ± 0.282
4.365 ± 0.88
4.186 ± 0.5
2.97 ± 0.413
6.869 ± 0.553
6.512 ± 0.573
5.367 ± 0.449
6.261 ± 0.427
1.145 ± 0.156
3.47 ± 0.454
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here