Amino acid dipepetide frequency for Propionibacterium phage G4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.519AlaAla: 18.519 ± 2.05
1.163AlaCys: 1.163 ± 0.361
8.387AlaAsp: 8.387 ± 0.947
6.228AlaGlu: 6.228 ± 0.867
2.74AlaPhe: 2.74 ± 0.629
10.214AlaGly: 10.214 ± 1.071
1.661AlaHis: 1.661 ± 0.347
5.232AlaIle: 5.232 ± 0.872
4.733AlaLys: 4.733 ± 0.639
11.709AlaLeu: 11.709 ± 1.178
4.318AlaMet: 4.318 ± 0.69
2.657AlaAsn: 2.657 ± 0.495
6.643AlaPro: 6.643 ± 0.634
4.484AlaGln: 4.484 ± 0.698
8.886AlaArg: 8.886 ± 1.375
9.052AlaSer: 9.052 ± 0.972
8.221AlaThr: 8.221 ± 0.836
7.723AlaVal: 7.723 ± 0.752
3.903AlaTrp: 3.903 ± 0.733
1.329AlaTyr: 1.329 ± 0.351
0.0AlaXaa: 0.0 ± 0.0
Cys
0.997CysAla: 0.997 ± 0.32
0.083CysCys: 0.083 ± 0.082
0.83CysAsp: 0.83 ± 0.288
0.083CysGlu: 0.083 ± 0.085
0.083CysPhe: 0.083 ± 0.091
1.163CysGly: 1.163 ± 0.424
0.581CysHis: 0.581 ± 0.234
0.332CysIle: 0.332 ± 0.155
0.498CysLys: 0.498 ± 0.182
0.664CysLeu: 0.664 ± 0.231
0.166CysMet: 0.166 ± 0.112
0.332CysAsn: 0.332 ± 0.163
1.246CysPro: 1.246 ± 0.357
0.166CysGln: 0.166 ± 0.111
0.997CysArg: 0.997 ± 0.277
0.415CysSer: 0.415 ± 0.172
0.581CysThr: 0.581 ± 0.213
0.166CysVal: 0.166 ± 0.112
0.581CysTrp: 0.581 ± 0.248
0.083CysTyr: 0.083 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
8.886AspAla: 8.886 ± 1.011
0.83AspCys: 0.83 ± 0.238
3.737AspAsp: 3.737 ± 0.572
4.318AspGlu: 4.318 ± 0.748
1.661AspPhe: 1.661 ± 0.45
7.225AspGly: 7.225 ± 0.778
1.246AspHis: 1.246 ± 0.31
2.906AspIle: 2.906 ± 0.481
1.827AspLys: 1.827 ± 0.356
6.643AspLeu: 6.643 ± 0.876
1.578AspMet: 1.578 ± 0.313
0.664AspAsn: 0.664 ± 0.229
4.069AspPro: 4.069 ± 0.729
1.661AspGln: 1.661 ± 0.467
5.149AspArg: 5.149 ± 0.595
3.073AspSer: 3.073 ± 0.469
3.405AspThr: 3.405 ± 0.496
4.567AspVal: 4.567 ± 0.694
1.246AspTrp: 1.246 ± 0.412
1.246AspTyr: 1.246 ± 0.359
0.0AspXaa: 0.0 ± 0.0
Glu
6.56GluAla: 6.56 ± 0.736
0.415GluCys: 0.415 ± 0.222
3.322GluAsp: 3.322 ± 0.552
2.491GluGlu: 2.491 ± 0.674
1.329GluPhe: 1.329 ± 0.302
2.99GluGly: 2.99 ± 0.699
1.329GluHis: 1.329 ± 0.28
2.491GluIle: 2.491 ± 0.366
1.495GluLys: 1.495 ± 0.417
5.813GluLeu: 5.813 ± 0.835
0.83GluMet: 0.83 ± 0.225
0.747GluAsn: 0.747 ± 0.25
2.491GluPro: 2.491 ± 0.469
2.408GluGln: 2.408 ± 0.455
4.235GluArg: 4.235 ± 0.852
2.906GluSer: 2.906 ± 0.506
3.405GluThr: 3.405 ± 0.528
3.737GluVal: 3.737 ± 0.786
1.661GluTrp: 1.661 ± 0.27
0.415GluTyr: 0.415 ± 0.209
0.0GluXaa: 0.0 ± 0.0
Phe
2.74PheAla: 2.74 ± 0.6
0.332PheCys: 0.332 ± 0.152
1.661PheAsp: 1.661 ± 0.381
1.495PheGlu: 1.495 ± 0.311
0.581PhePhe: 0.581 ± 0.208
3.073PheGly: 3.073 ± 0.717
0.332PheHis: 0.332 ± 0.163
1.08PheIle: 1.08 ± 0.314
0.997PheLys: 0.997 ± 0.217
1.246PheLeu: 1.246 ± 0.328
1.329PheMet: 1.329 ± 0.322
0.249PheAsn: 0.249 ± 0.214
1.08PhePro: 1.08 ± 0.276
1.495PheGln: 1.495 ± 0.29
1.993PheArg: 1.993 ± 0.376
1.329PheSer: 1.329 ± 0.469
2.159PheThr: 2.159 ± 0.353
1.329PheVal: 1.329 ± 0.283
0.498PheTrp: 0.498 ± 0.19
0.581PheTyr: 0.581 ± 0.309
0.0PheXaa: 0.0 ± 0.0
Gly
9.882GlyAla: 9.882 ± 1.161
0.83GlyCys: 0.83 ± 0.295
4.318GlyAsp: 4.318 ± 0.643
4.65GlyGlu: 4.65 ± 0.568
2.657GlyPhe: 2.657 ± 0.55
7.64GlyGly: 7.64 ± 1.044
1.744GlyHis: 1.744 ± 0.456
4.235GlyIle: 4.235 ± 0.627
2.823GlyLys: 2.823 ± 0.387
8.055GlyLeu: 8.055 ± 1.01
1.495GlyMet: 1.495 ± 0.455
1.91GlyAsn: 1.91 ± 0.325
3.82GlyPro: 3.82 ± 0.587
2.657GlyGln: 2.657 ± 0.795
5.896GlyArg: 5.896 ± 0.699
4.9GlySer: 4.9 ± 0.962
5.896GlyThr: 5.896 ± 0.644
6.311GlyVal: 6.311 ± 0.823
2.823GlyTrp: 2.823 ± 0.486
1.661GlyTyr: 1.661 ± 0.335
0.0GlyXaa: 0.0 ± 0.0
His
2.076HisAla: 2.076 ± 0.439
0.498HisCys: 0.498 ± 0.183
1.163HisAsp: 1.163 ± 0.373
1.163HisGlu: 1.163 ± 0.279
0.498HisPhe: 0.498 ± 0.234
1.412HisGly: 1.412 ± 0.327
0.581HisHis: 0.581 ± 0.248
0.581HisIle: 0.581 ± 0.231
0.581HisLys: 0.581 ± 0.213
1.329HisLeu: 1.329 ± 0.366
0.166HisMet: 0.166 ± 0.103
0.166HisAsn: 0.166 ± 0.133
1.246HisPro: 1.246 ± 0.41
0.747HisGln: 0.747 ± 0.198
1.246HisArg: 1.246 ± 0.349
1.246HisSer: 1.246 ± 0.359
1.495HisThr: 1.495 ± 0.416
1.578HisVal: 1.578 ± 0.388
0.997HisTrp: 0.997 ± 0.297
0.249HisTyr: 0.249 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
5.73IleAla: 5.73 ± 0.66
0.249IleCys: 0.249 ± 0.159
4.401IleAsp: 4.401 ± 0.613
3.239IleGlu: 3.239 ± 0.575
1.246IlePhe: 1.246 ± 0.422
3.488IleGly: 3.488 ± 1.296
0.913IleHis: 0.913 ± 0.279
2.823IleIle: 2.823 ± 0.557
1.08IleLys: 1.08 ± 0.268
2.99IleLeu: 2.99 ± 0.623
0.83IleMet: 0.83 ± 0.262
0.83IleAsn: 0.83 ± 0.251
2.657IlePro: 2.657 ± 0.463
2.159IleGln: 2.159 ± 0.4
2.74IleArg: 2.74 ± 0.634
2.906IleSer: 2.906 ± 0.801
4.65IleThr: 4.65 ± 0.492
3.405IleVal: 3.405 ± 0.566
1.08IleTrp: 1.08 ± 0.326
0.997IleTyr: 0.997 ± 0.316
0.0IleXaa: 0.0 ± 0.0
Lys
4.733LysAla: 4.733 ± 0.746
0.166LysCys: 0.166 ± 0.11
1.744LysAsp: 1.744 ± 0.325
0.997LysGlu: 0.997 ± 0.265
0.997LysPhe: 0.997 ± 0.398
2.325LysGly: 2.325 ± 0.41
0.498LysHis: 0.498 ± 0.221
1.163LysIle: 1.163 ± 0.342
0.747LysLys: 0.747 ± 0.237
2.74LysLeu: 2.74 ± 0.383
0.581LysMet: 0.581 ± 0.195
0.581LysAsn: 0.581 ± 0.195
2.242LysPro: 2.242 ± 0.488
1.661LysGln: 1.661 ± 0.4
3.571LysArg: 3.571 ± 0.738
1.661LysSer: 1.661 ± 0.317
2.74LysThr: 2.74 ± 0.524
2.574LysVal: 2.574 ± 0.458
0.498LysTrp: 0.498 ± 0.208
0.581LysTyr: 0.581 ± 0.202
0.0LysXaa: 0.0 ± 0.0
Leu
10.879LeuAla: 10.879 ± 1.211
0.913LeuCys: 0.913 ± 0.302
5.813LeuAsp: 5.813 ± 0.723
3.405LeuGlu: 3.405 ± 0.717
2.408LeuPhe: 2.408 ± 0.412
7.889LeuGly: 7.889 ± 0.906
1.495LeuHis: 1.495 ± 0.353
4.65LeuIle: 4.65 ± 0.849
2.823LeuLys: 2.823 ± 0.48
5.813LeuLeu: 5.813 ± 0.781
2.491LeuMet: 2.491 ± 0.506
1.163LeuAsn: 1.163 ± 0.338
4.484LeuPro: 4.484 ± 0.597
3.073LeuGln: 3.073 ± 0.532
5.398LeuArg: 5.398 ± 0.718
6.145LeuSer: 6.145 ± 0.933
7.142LeuThr: 7.142 ± 0.865
5.564LeuVal: 5.564 ± 0.695
1.744LeuTrp: 1.744 ± 0.371
0.664LeuTyr: 0.664 ± 0.234
0.0LeuXaa: 0.0 ± 0.0
Met
4.318MetAla: 4.318 ± 0.641
0.0MetCys: 0.0 ± 0.0
1.412MetAsp: 1.412 ± 0.346
0.83MetGlu: 0.83 ± 0.271
0.249MetPhe: 0.249 ± 0.133
1.578MetGly: 1.578 ± 0.376
0.415MetHis: 0.415 ± 0.206
1.329MetIle: 1.329 ± 0.269
1.08MetLys: 1.08 ± 0.29
1.91MetLeu: 1.91 ± 0.447
0.664MetMet: 0.664 ± 0.312
0.581MetAsn: 0.581 ± 0.273
1.246MetPro: 1.246 ± 0.403
0.747MetGln: 0.747 ± 0.215
1.329MetArg: 1.329 ± 0.363
2.574MetSer: 2.574 ± 0.467
1.827MetThr: 1.827 ± 0.336
1.495MetVal: 1.495 ± 0.342
0.332MetTrp: 0.332 ± 0.165
0.166MetTyr: 0.166 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
2.242AsnAla: 2.242 ± 0.405
0.0AsnCys: 0.0 ± 0.0
0.913AsnAsp: 0.913 ± 0.281
0.166AsnGlu: 0.166 ± 0.124
0.415AsnPhe: 0.415 ± 0.172
1.91AsnGly: 1.91 ± 0.346
0.498AsnHis: 0.498 ± 0.18
1.246AsnIle: 1.246 ± 0.28
0.332AsnLys: 0.332 ± 0.149
1.495AsnLeu: 1.495 ± 0.422
0.415AsnMet: 0.415 ± 0.177
0.747AsnAsn: 0.747 ± 0.305
1.91AsnPro: 1.91 ± 0.397
0.498AsnGln: 0.498 ± 0.217
1.495AsnArg: 1.495 ± 0.338
0.997AsnSer: 0.997 ± 0.345
0.747AsnThr: 0.747 ± 0.238
1.578AsnVal: 1.578 ± 0.312
0.581AsnTrp: 0.581 ± 0.361
0.249AsnTyr: 0.249 ± 0.144
0.0AsnXaa: 0.0 ± 0.0
Pro
7.308ProAla: 7.308 ± 0.993
0.498ProCys: 0.498 ± 0.218
5.149ProAsp: 5.149 ± 0.771
4.318ProGlu: 4.318 ± 0.679
1.661ProPhe: 1.661 ± 0.472
4.816ProGly: 4.816 ± 0.803
1.246ProHis: 1.246 ± 0.378
2.076ProIle: 2.076 ± 0.418
2.076ProLys: 2.076 ± 0.463
3.571ProLeu: 3.571 ± 0.624
0.913ProMet: 0.913 ± 0.301
1.163ProAsn: 1.163 ± 0.397
3.239ProPro: 3.239 ± 0.723
1.578ProGln: 1.578 ± 0.418
2.325ProArg: 2.325 ± 0.627
3.571ProSer: 3.571 ± 0.628
4.069ProThr: 4.069 ± 0.66
5.066ProVal: 5.066 ± 1.131
0.913ProTrp: 0.913 ± 0.282
0.415ProTyr: 0.415 ± 0.16
0.0ProXaa: 0.0 ± 0.0
Gln
5.066GlnAla: 5.066 ± 0.478
0.332GlnCys: 0.332 ± 0.183
1.661GlnAsp: 1.661 ± 0.377
1.246GlnGlu: 1.246 ± 0.489
1.163GlnPhe: 1.163 ± 0.299
2.491GlnGly: 2.491 ± 0.427
0.913GlnHis: 0.913 ± 0.233
2.657GlnIle: 2.657 ± 0.538
1.163GlnLys: 1.163 ± 0.296
4.484GlnLeu: 4.484 ± 0.914
0.664GlnMet: 0.664 ± 0.301
0.415GlnAsn: 0.415 ± 0.182
1.412GlnPro: 1.412 ± 0.275
1.495GlnGln: 1.495 ± 0.321
2.823GlnArg: 2.823 ± 0.58
2.076GlnSer: 2.076 ± 0.422
1.827GlnThr: 1.827 ± 0.5
4.152GlnVal: 4.152 ± 0.664
0.913GlnTrp: 0.913 ± 0.304
0.249GlnTyr: 0.249 ± 0.132
0.0GlnXaa: 0.0 ± 0.0
Arg
7.059ArgAla: 7.059 ± 0.991
1.246ArgCys: 1.246 ± 0.421
5.481ArgAsp: 5.481 ± 0.688
3.903ArgGlu: 3.903 ± 0.599
2.076ArgPhe: 2.076 ± 0.52
5.232ArgGly: 5.232 ± 0.804
1.412ArgHis: 1.412 ± 0.325
2.823ArgIle: 2.823 ± 0.454
2.242ArgLys: 2.242 ± 0.513
5.149ArgLeu: 5.149 ± 0.759
1.329ArgMet: 1.329 ± 0.362
1.412ArgAsn: 1.412 ± 0.326
4.069ArgPro: 4.069 ± 0.888
3.073ArgGln: 3.073 ± 0.537
6.477ArgArg: 6.477 ± 1.22
3.986ArgSer: 3.986 ± 0.562
4.816ArgThr: 4.816 ± 0.8
5.232ArgVal: 5.232 ± 0.748
2.325ArgTrp: 2.325 ± 0.555
1.08ArgTyr: 1.08 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
8.221SerAla: 8.221 ± 1.244
0.498SerCys: 0.498 ± 0.253
4.152SerAsp: 4.152 ± 0.596
3.156SerGlu: 3.156 ± 0.779
1.412SerPhe: 1.412 ± 0.357
5.979SerGly: 5.979 ± 0.776
0.664SerHis: 0.664 ± 0.262
3.571SerIle: 3.571 ± 0.528
1.744SerLys: 1.744 ± 0.345
5.315SerLeu: 5.315 ± 0.683
1.827SerMet: 1.827 ± 0.438
1.163SerAsn: 1.163 ± 0.282
2.99SerPro: 2.99 ± 0.49
2.159SerGln: 2.159 ± 0.413
3.903SerArg: 3.903 ± 0.502
4.401SerSer: 4.401 ± 1.055
4.567SerThr: 4.567 ± 0.622
4.567SerVal: 4.567 ± 0.748
1.91SerTrp: 1.91 ± 0.545
0.83SerTyr: 0.83 ± 0.297
0.0SerXaa: 0.0 ± 0.0
Thr
7.723ThrAla: 7.723 ± 0.888
1.246ThrCys: 1.246 ± 0.42
5.149ThrAsp: 5.149 ± 0.735
2.076ThrGlu: 2.076 ± 0.432
1.578ThrPhe: 1.578 ± 0.376
6.062ThrGly: 6.062 ± 0.589
1.08ThrHis: 1.08 ± 0.324
4.235ThrIle: 4.235 ± 0.703
2.74ThrLys: 2.74 ± 0.478
6.311ThrLeu: 6.311 ± 0.664
1.661ThrMet: 1.661 ± 0.359
0.913ThrAsn: 0.913 ± 0.204
4.816ThrPro: 4.816 ± 0.826
2.159ThrGln: 2.159 ± 0.413
3.571ThrArg: 3.571 ± 0.674
4.318ThrSer: 4.318 ± 0.777
5.564ThrThr: 5.564 ± 0.918
5.73ThrVal: 5.73 ± 0.884
1.827ThrTrp: 1.827 ± 0.428
0.913ThrTyr: 0.913 ± 0.312
0.0ThrXaa: 0.0 ± 0.0
Val
10.38ValAla: 10.38 ± 0.863
0.581ValCys: 0.581 ± 0.235
3.737ValAsp: 3.737 ± 0.588
5.398ValGlu: 5.398 ± 0.721
2.159ValPhe: 2.159 ± 0.576
5.73ValGly: 5.73 ± 1.397
1.495ValHis: 1.495 ± 0.402
3.571ValIle: 3.571 ± 0.704
2.325ValLys: 2.325 ± 0.416
5.564ValLeu: 5.564 ± 0.971
1.661ValMet: 1.661 ± 0.318
1.495ValAsn: 1.495 ± 0.306
4.152ValPro: 4.152 ± 0.531
2.657ValGln: 2.657 ± 0.398
4.567ValArg: 4.567 ± 0.663
5.315ValSer: 5.315 ± 0.853
4.401ValThr: 4.401 ± 0.6
5.149ValVal: 5.149 ± 1.109
1.744ValTrp: 1.744 ± 0.395
0.83ValTyr: 0.83 ± 0.255
0.0ValXaa: 0.0 ± 0.0
Trp
2.408TrpAla: 2.408 ± 0.477
0.0TrpCys: 0.0 ± 0.0
2.491TrpAsp: 2.491 ± 0.373
1.329TrpGlu: 1.329 ± 0.373
0.166TrpPhe: 0.166 ± 0.109
1.578TrpGly: 1.578 ± 0.415
0.664TrpHis: 0.664 ± 0.278
1.163TrpIle: 1.163 ± 0.264
0.83TrpLys: 0.83 ± 0.263
2.076TrpLeu: 2.076 ± 0.431
0.997TrpMet: 0.997 ± 0.256
0.664TrpAsn: 0.664 ± 0.222
1.329TrpPro: 1.329 ± 0.291
1.329TrpGln: 1.329 ± 0.386
3.073TrpArg: 3.073 ± 0.725
1.495TrpSer: 1.495 ± 0.462
1.661TrpThr: 1.661 ± 0.431
2.242TrpVal: 2.242 ± 0.4
0.747TrpTrp: 0.747 ± 0.236
0.249TrpTyr: 0.249 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.076TyrAla: 2.076 ± 0.43
0.166TyrCys: 0.166 ± 0.135
0.415TyrAsp: 0.415 ± 0.174
0.581TyrGlu: 0.581 ± 0.317
0.498TyrPhe: 0.498 ± 0.198
1.495TyrGly: 1.495 ± 0.383
0.249TyrHis: 0.249 ± 0.185
0.249TyrIle: 0.249 ± 0.147
0.664TyrLys: 0.664 ± 0.227
1.08TyrLeu: 1.08 ± 0.323
0.083TyrMet: 0.083 ± 0.077
0.581TyrAsn: 0.581 ± 0.209
0.83TyrPro: 0.83 ± 0.3
0.997TyrGln: 0.997 ± 0.358
0.747TyrArg: 0.747 ± 0.278
0.664TyrSer: 0.664 ± 0.251
0.498TyrThr: 0.498 ± 0.165
0.747TyrVal: 0.747 ± 0.225
0.166TyrTrp: 0.166 ± 0.131
0.166TyrTyr: 0.166 ± 0.117
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (12043 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski