Dickeya phage vB_DsoM_AD1
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 330 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A384ZYF9|A0A384ZYF9_9CAUD Putative DNA primase OS=Dickeya phage vB_DsoM_AD1 OX=2283029 GN=AD1_223 PE=4 SV=1
MM1 pKa = 7.69 GDD3 pKa = 3.39 EE4 pKa = 5.12 DD5 pKa = 5.32 FDD7 pKa = 5.06 FEE9 pKa = 5.49 FGADD13 pKa = 4.09 DD14 pKa = 4.45 DD15 pKa = 4.13 SGYY18 pKa = 11.32 YY19 pKa = 9.29 IDD21 pKa = 5.41 QPLHH25 pKa = 6.11 MIVDD29 pKa = 3.74 GDD31 pKa = 3.81 EE32 pKa = 4.85 RR33 pKa = 11.84 ILGHH37 pKa = 7.21 LIDD40 pKa = 5.04 SGDD43 pKa = 3.53 TVLMYY48 pKa = 10.77 GGYY51 pKa = 10.11 YY52 pKa = 10.17 VVDD55 pKa = 4.27 DD56 pKa = 5.07 EE57 pKa = 5.13 EE58 pKa = 6.44 DD59 pKa = 3.55 PDD61 pKa = 3.94 AATLIEE67 pKa = 4.26 VPVFKK72 pKa = 10.65 VDD74 pKa = 3.71 PLADD78 pKa = 3.03 IAIVMNADD86 pKa = 3.5 EE87 pKa = 4.46 MRR89 pKa = 11.84 LRR91 pKa = 11.84 HH92 pKa = 5.99 NGEE95 pKa = 4.23 LLTLAFINDD104 pKa = 3.07 IPLVVKK110 pKa = 7.89 STRR113 pKa = 11.84 HH114 pKa = 4.25
Molecular weight: 12.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.017
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.554
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.757
Sillero 3.91
Patrickios 3.083
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.848
Protein with the highest isoelectric point:
>tr|A0A384ZY35|A0A384ZY35_9CAUD Putative tail protein OS=Dickeya phage vB_DsoM_AD1 OX=2283029 GN=AD1_108 PE=4 SV=1
MM1 pKa = 6.66 GQKK4 pKa = 10.47 AIRR7 pKa = 11.84 LGTDD11 pKa = 3.55 LSTGHH16 pKa = 6.4 NGYY19 pKa = 10.08 FPVVPAQASSNVLVNGKK36 pKa = 10.4 GSVRR40 pKa = 11.84 QGDD43 pKa = 4.03 SYY45 pKa = 11.24 RR46 pKa = 11.84 PHH48 pKa = 7.32 WKK50 pKa = 9.43 PKK52 pKa = 9.95 KK53 pKa = 9.46 PPHH56 pKa = 5.79 TGKK59 pKa = 8.83 ATSSSSVRR67 pKa = 11.84 VNGKK71 pKa = 7.85 PAQRR75 pKa = 11.84 AGDD78 pKa = 3.94 ANSCGDD84 pKa = 3.53 TASNGSSNVRR94 pKa = 11.84 FGG96 pKa = 3.28
Molecular weight: 9.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.293
IPC2_protein 9.78
IPC_protein 10.218
Toseland 10.789
ProMoST 10.35
Dawson 10.862
Bjellqvist 10.496
Wikipedia 11.008
Rodwell 11.316
Grimsley 10.891
Solomon 10.935
Lehninger 10.921
Nozaki 10.76
DTASelect 10.496
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 11.082
IPC_peptide 10.95
IPC2_peptide 9.268
IPC2.peptide.svr19 8.625
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
330
0
330
81997
41
1948
248.5
27.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.488 ± 0.142
1.163 ± 0.058
6.877 ± 0.163
6.183 ± 0.179
3.762 ± 0.075
6.551 ± 0.144
2.056 ± 0.083
5.426 ± 0.079
6.279 ± 0.255
8.192 ± 0.125
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.831 ± 0.087
4.592 ± 0.088
4.332 ± 0.086
3.759 ± 0.095
5.347 ± 0.125
6.25 ± 0.12
6.129 ± 0.184
7.192 ± 0.105
1.148 ± 0.05
3.443 ± 0.091
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here