Cinara cedri
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22140 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5E4M159|A0A5E4M159_9HEMI Isoform of A0A5E4M372 Uncharacterized protein OS=Cinara cedri OX=506608 GN=CINCED_3A021463 PE=4 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 LFLYY6 pKa = 9.78 TLLVSTLAISKK17 pKa = 7.37 TVRR20 pKa = 11.84 TDD22 pKa = 2.91 NPQPSDD28 pKa = 3.43 QTNLEE33 pKa = 4.19 LSVSNDD39 pKa = 2.97 QSNSYY44 pKa = 10.41 QNQNSLKK51 pKa = 10.65 ADD53 pKa = 3.37 VTNDD57 pKa = 3.22 GKK59 pKa = 10.26 HH60 pKa = 5.97 ASLDD64 pKa = 3.55 ASSQTVQNSQSKK76 pKa = 10.82 NEE78 pKa = 3.72 LHH80 pKa = 7.09 LNIEE84 pKa = 4.21 QSDD87 pKa = 4.0 TDD89 pKa = 3.91 SDD91 pKa = 4.63 PNDD94 pKa = 3.52 STISNSAMTNEE105 pKa = 5.09 NINTLQQQSYY115 pKa = 9.19 NFKK118 pKa = 10.38 EE119 pKa = 4.49 SNSLLLNMQNNNIDD133 pKa = 3.53 NDD135 pKa = 4.08 NNNSPDD141 pKa = 3.42 SGTDD145 pKa = 3.32 DD146 pKa = 5.18 LEE148 pKa = 5.02 SNNPTGQNFGLISPQPDD165 pKa = 3.45 GASPQILGSGIQTNSPTQGQNIMSLSGTDD194 pKa = 3.34 PNSINNADD202 pKa = 4.04 AYY204 pKa = 10.81 GPTQPRR210 pKa = 11.84 DD211 pKa = 3.32 SSSPQGSPINYY222 pKa = 9.13 NDD224 pKa = 4.54 DD225 pKa = 4.58 NIPTAGGSSNGNTPPSSGGTTSPDD249 pKa = 2.9 SSQIGSGSLPISTSPGGQNGLNPSLFDD276 pKa = 4.4 PNSPNDD282 pKa = 3.59 AGTFGPSQFPDD293 pKa = 3.13 SSSLQGSPNNNNNDD307 pKa = 4.09 NIPPAGGISNGNTPPSSGGPASPDD331 pKa = 3.16 SPQIGSGSLPISTSPGGQNGLNPSLFDD358 pKa = 4.4 PNSPNDD364 pKa = 3.59 AGTFGPSQFPDD375 pKa = 3.13 SSSLQGSPNNNNNDD389 pKa = 4.09 NIPPAGGSSNGNTPPSSGGPASPSYY414 pKa = 9.33 PQFGIGLVPLSYY426 pKa = 10.61 LPSGQNGVNPSLFDD440 pKa = 4.33 PNSINNAGAFGPSQPPDD457 pKa = 3.22 SSSPQGSPNNNNNDD471 pKa = 4.09 NIPPAGGSSNGNTPPSSGGPASPDD495 pKa = 3.16 SPQIGSGSIPISTSPGGQNGLNPSLFDD522 pKa = 4.4 PNSPNDD528 pKa = 3.75 AGSNDD533 pKa = 3.68 AGSFGPSQFPDD544 pKa = 3.13 SSSLQGSPNNNNNDD558 pKa = 4.09 NIPPAGGLSNGNSPPSSEE576 pKa = 4.09 GSSTVTNLYY585 pKa = 8.82 TKK587 pKa = 10.52 SSEE590 pKa = 4.18 LSNSEE595 pKa = 4.02 YY596 pKa = 10.8 FNGGVSHH603 pKa = 6.68 SEE605 pKa = 3.6 SSQFEE610 pKa = 3.93 EE611 pKa = 4.94 SISITSDD618 pKa = 2.94 STEE621 pKa = 4.23 MEE623 pKa = 4.05 TQQYY627 pKa = 9.6 VLNSYY632 pKa = 10.96 SSGKK636 pKa = 9.69 FICDD640 pKa = 3.34 GYY642 pKa = 11.28 GKK644 pKa = 10.42 IIGVVFNHH652 pKa = 6.4 SADD655 pKa = 3.56 SGQGLDD661 pKa = 5.1 TIDD664 pKa = 5.27 LDD666 pKa = 4.22 TNSVIWEE673 pKa = 4.75 DD674 pKa = 3.53 INMLGLDD681 pKa = 3.78 IYY683 pKa = 10.97 QFSGNAGNYY692 pKa = 10.02 VGDD695 pKa = 4.21 KK696 pKa = 10.74 ADD698 pKa = 3.44 NAYY701 pKa = 9.36 TSSKK705 pKa = 10.91 NSLGDD710 pKa = 3.25 AANYY714 pKa = 10.39 VGDD717 pKa = 4.29 KK718 pKa = 10.94 ADD720 pKa = 3.58 NAYY723 pKa = 7.7 TASKK727 pKa = 10.88 NSLGDD732 pKa = 3.26 AANYY736 pKa = 10.39 VGDD739 pKa = 4.29 KK740 pKa = 10.83 ADD742 pKa = 3.44 NAYY745 pKa = 9.36 TSSKK749 pKa = 10.91 NSLGDD754 pKa = 3.49 AANHH758 pKa = 6.35 VGDD761 pKa = 4.44 IADD764 pKa = 3.64 NAYY767 pKa = 9.31 TSSKK771 pKa = 10.91 NSLGDD776 pKa = 3.25 AANYY780 pKa = 10.39 VGDD783 pKa = 3.83 KK784 pKa = 10.93 AGAVNDD790 pKa = 4.0 ASKK793 pKa = 10.74 EE794 pKa = 4.04 AGNSIHH800 pKa = 7.01 SATNDD805 pKa = 3.62 YY806 pKa = 11.37 GHH808 pKa = 7.5 DD809 pKa = 4.03 LYY811 pKa = 11.44 DD812 pKa = 3.96 AGHH815 pKa = 6.21 NFKK818 pKa = 10.84 EE819 pKa = 4.25 NAFGSSDD826 pKa = 3.38 GDD828 pKa = 3.59 VTNNVV833 pKa = 3.18
Molecular weight: 85.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.674
IPC2_protein 3.617
IPC_protein 3.681
Toseland 3.439
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.668
Rodwell 3.503
Grimsley 3.338
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.126
Thurlkill 3.503
EMBOSS 3.668
Sillero 3.808
Patrickios 1.163
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.739
Protein with the highest isoelectric point:
>tr|A0A5E4NGA4|A0A5E4NGA4_9HEMI 39S ribosomal protein L28 mitochondrial OS=Cinara cedri OX=506608 GN=CINCED_3A006303 PE=3 SV=1
MM1 pKa = 7.65 GGQKK5 pKa = 9.56 KK6 pKa = 9.38 AAAEE10 pKa = 3.99 TLSLGGGGPRR20 pKa = 11.84 TPGVYY25 pKa = 9.15 TRR27 pKa = 11.84 AAFFNSAPGQTHH39 pKa = 5.34 CRR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 TTRR49 pKa = 11.84 CRR51 pKa = 11.84 FVAPRR56 pKa = 11.84 TTPPPVVYY64 pKa = 9.15 PAFPLRR70 pKa = 11.84 SIRR73 pKa = 11.84 SPPPPPPPPKK83 pKa = 10.35 SPPEE87 pKa = 4.17 HH88 pKa = 6.18 VCSTTKK94 pKa = 10.13 GHH96 pKa = 6.93 CASQPLAPGKK106 pKa = 9.86 WNDD109 pKa = 2.71 IFFGIFRR116 pKa = 11.84 DD117 pKa = 3.88 STHH120 pKa = 7.43 DD121 pKa = 3.38 EE122 pKa = 4.51 LYY124 pKa = 10.25 TLGFRR129 pKa = 11.84 EE130 pKa = 4.1 RR131 pKa = 11.84 PLDD134 pKa = 3.73 EE135 pKa = 5.2 KK136 pKa = 11.11 SRR138 pKa = 11.84 CDD140 pKa = 3.14 RR141 pKa = 11.84 TVPSGDD147 pKa = 3.34 VFSS150 pKa = 4.53
Molecular weight: 16.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 9.545
IPC_protein 10.365
Toseland 10.613
ProMoST 10.335
Dawson 10.716
Bjellqvist 10.452
Wikipedia 10.935
Rodwell 10.862
Grimsley 10.76
Solomon 10.847
Lehninger 10.818
Nozaki 10.643
DTASelect 10.438
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 10.599
IPC_peptide 10.847
IPC2_peptide 9.78
IPC2.peptide.svr19 8.537
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16923
5217
22140
10829743
38
16606
489.1
55.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.286 ± 0.018
2.051 ± 0.034
5.558 ± 0.013
6.231 ± 0.019
3.914 ± 0.015
5.058 ± 0.023
2.426 ± 0.008
6.562 ± 0.019
7.139 ± 0.023
8.952 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.379 ± 0.008
6.232 ± 0.018
4.742 ± 0.026
4.167 ± 0.021
4.637 ± 0.017
8.209 ± 0.021
5.957 ± 0.013
6.028 ± 0.014
1.055 ± 0.007
3.398 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here