Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) (Chromatium vinosum)
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3211 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D3RVH2|D3RVH2_ALLVD Phospho-N-acetylmuramoyl-pentapeptide-transferase OS=Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) OX=572477 GN=mraY PE=3 SV=1
MM1 pKa = 7.11 NAEE4 pKa = 4.51 ADD6 pKa = 3.86 LEE8 pKa = 4.41 APLVFTASAASKK20 pKa = 9.42 VAEE23 pKa = 4.88 LIRR26 pKa = 11.84 DD27 pKa = 3.59 EE28 pKa = 4.65 GNPGLMLRR36 pKa = 11.84 VYY38 pKa = 9.87 IQGGGCSGFQYY49 pKa = 10.98 GFTFDD54 pKa = 5.04 EE55 pKa = 4.86 DD56 pKa = 4.03 VQDD59 pKa = 3.89 GDD61 pKa = 4.33 TEE63 pKa = 4.53 VVTDD67 pKa = 4.35 GVKK70 pKa = 10.81 LLVDD74 pKa = 4.06 PMSLQYY80 pKa = 11.73 LMGAEE85 pKa = 3.76 IDD87 pKa = 3.97 YY88 pKa = 10.81 TEE90 pKa = 4.56 GLQGAQFVIRR100 pKa = 11.84 NPNATTTCGCGSSFSAA116 pKa = 4.79
Molecular weight: 12.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.528
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.846
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|D3RSC0|D3RSC0_ALLVD Transposase IS605 OrfB family OS=Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) OX=572477 GN=Alvin_1140 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 LKK11 pKa = 10.48 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.93 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 SATRR25 pKa = 11.84 NGRR28 pKa = 11.84 KK29 pKa = 9.31 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.62 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LIPP44 pKa = 3.91
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3211
0
3211
1097853
30
3764
341.9
37.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.156 ± 0.044
0.965 ± 0.017
5.894 ± 0.037
6.569 ± 0.044
3.337 ± 0.027
7.895 ± 0.044
2.317 ± 0.02
4.98 ± 0.029
2.559 ± 0.031
11.637 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.112 ± 0.023
2.398 ± 0.028
5.35 ± 0.035
3.703 ± 0.025
8.175 ± 0.056
5.21 ± 0.034
5.137 ± 0.041
6.917 ± 0.035
1.399 ± 0.018
2.289 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here