Mycobacterium phage Bernal13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bernalvirus; Mycobacterium virus Bernal13

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A023W6M4|A0A023W6M4_9CAUD RecB-like exonuclease/helicase OS=Mycobacterium phage Bernal13 OX=1486424 GN=44 PE=4 SV=1
MM1 pKa = 7.2TALEE5 pKa = 4.37LLGVLAVAALVSVSVLYY22 pKa = 10.86ALAGLIRR29 pKa = 11.84RR30 pKa = 11.84DD31 pKa = 5.49LEE33 pKa = 4.61DD34 pKa = 3.84QDD36 pKa = 5.64DD37 pKa = 4.19DD38 pKa = 4.03AALYY42 pKa = 9.48PSEE45 pKa = 4.83YY46 pKa = 9.52YY47 pKa = 10.69GSYY50 pKa = 10.64LVTLTAEE57 pKa = 4.08QAAGMRR63 pKa = 11.84FTFSDD68 pKa = 3.52GSYY71 pKa = 10.52RR72 pKa = 11.84EE73 pKa = 4.18LAADD77 pKa = 4.43GHH79 pKa = 5.85WEE81 pKa = 3.83WHH83 pKa = 6.2PAPDD87 pKa = 4.14TEE89 pKa = 4.57TASADD94 pKa = 3.46PTRR97 pKa = 4.85

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A023W5X1|A0A023W5X1_9CAUD Uncharacterized protein OS=Mycobacterium phage Bernal13 OX=1486424 GN=30 PE=4 SV=1
MM1 pKa = 7.4VDD3 pKa = 3.25SAPVKK8 pKa = 10.53FGTAGAFVYY17 pKa = 10.59NVAQDD22 pKa = 3.38ATHH25 pKa = 6.7YY26 pKa = 11.05DD27 pKa = 3.78VVLLPGGGGGNAGNSIGQDD46 pKa = 3.15GVGGFFGPFLTRR58 pKa = 11.84TLRR61 pKa = 11.84RR62 pKa = 11.84GIDD65 pKa = 3.4FGLDD69 pKa = 3.14VQQITGTVGAGGASATAGQASTANAGTVALSTPGGNPGSSWNGIGDD115 pKa = 3.43NAYY118 pKa = 9.43IGKK121 pKa = 9.93GPGSIVFNGRR131 pKa = 11.84TYY133 pKa = 10.2TGGGNVQTSAPSAGATGAAGAAPGGGGGGGGGVTFVGTPGKK174 pKa = 10.15GGRR177 pKa = 11.84GADD180 pKa = 3.41GAVWIFGYY188 pKa = 9.25NVKK191 pKa = 9.13STGTGSGSFGWGSGHH206 pKa = 6.61AAGRR210 pKa = 11.84RR211 pKa = 11.84IPKK214 pKa = 10.05AVGSGAFGWTGAAHH228 pKa = 7.02GVQPSKK234 pKa = 11.18GQGAGAFGWTASAGTGRR251 pKa = 11.84RR252 pKa = 11.84IPKK255 pKa = 9.03ACSRR259 pKa = 11.84GDD261 pKa = 3.8YY262 pKa = 9.72GWAGAGYY269 pKa = 9.49GHH271 pKa = 7.33EE272 pKa = 4.48GTPKK276 pKa = 10.36GIGSGVYY283 pKa = 9.82GWVGSAAGRR292 pKa = 11.84IDD294 pKa = 3.77PLGAGAGAYY303 pKa = 9.02AWLYY307 pKa = 9.18QPPASIARR315 pKa = 11.84HH316 pKa = 5.61RR317 pKa = 11.84LSIVPAVDD325 pKa = 3.11RR326 pKa = 11.84NTIVPAVDD334 pKa = 3.38RR335 pKa = 11.84AASVAVTDD343 pKa = 4.16RR344 pKa = 11.84GIEE347 pKa = 4.13VPHH350 pKa = 6.68PGPRR354 pKa = 11.84TAVVAAAPRR363 pKa = 11.84TIAVPDD369 pKa = 3.68VNRR372 pKa = 11.84SAVVSATDD380 pKa = 3.48RR381 pKa = 11.84RR382 pKa = 11.84VTANTTPDD390 pKa = 3.43RR391 pKa = 11.84TSMVPP396 pKa = 3.18

Molecular weight: 38.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13547

45

1361

225.8

24.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.977 ± 0.796

0.923 ± 0.146

6.769 ± 0.289

4.865 ± 0.44

2.82 ± 0.204

9.323 ± 0.852

1.941 ± 0.222

4.267 ± 0.197

3.012 ± 0.186

7.751 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.794 ± 0.19

3.263 ± 0.233

6.252 ± 0.236

4.51 ± 0.286

6.769 ± 0.476

5.403 ± 0.324

6.348 ± 0.305

6.961 ± 0.25

1.845 ± 0.224

2.207 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski