Amino acid dipepetide frequency for Streptococcus phage IPP54

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.057AlaAla: 4.057 ± 1.204
0.406AlaCys: 0.406 ± 0.171
5.68AlaAsp: 5.68 ± 1.217
5.68AlaGlu: 5.68 ± 0.917
3.002AlaPhe: 3.002 ± 0.391
3.814AlaGly: 3.814 ± 0.608
0.73AlaHis: 0.73 ± 0.216
5.031AlaIle: 5.031 ± 0.767
5.68AlaLys: 5.68 ± 0.769
6.086AlaLeu: 6.086 ± 0.978
1.785AlaMet: 1.785 ± 0.476
3.733AlaAsn: 3.733 ± 0.43
1.866AlaPro: 1.866 ± 0.365
2.84AlaGln: 2.84 ± 0.585
3.165AlaArg: 3.165 ± 0.621
4.463AlaSer: 4.463 ± 0.641
4.463AlaThr: 4.463 ± 0.7
4.706AlaVal: 4.706 ± 0.789
0.73AlaTrp: 0.73 ± 0.22
2.678AlaTyr: 2.678 ± 0.485
0.0AlaXaa: 0.0 ± 0.0
Cys
0.325CysAla: 0.325 ± 0.157
0.081CysCys: 0.081 ± 0.076
0.162CysAsp: 0.162 ± 0.114
0.73CysGlu: 0.73 ± 0.191
0.487CysPhe: 0.487 ± 0.223
0.325CysGly: 0.325 ± 0.155
0.081CysHis: 0.081 ± 0.092
0.406CysIle: 0.406 ± 0.184
0.406CysLys: 0.406 ± 0.165
0.325CysLeu: 0.325 ± 0.15
0.162CysMet: 0.162 ± 0.108
0.081CysAsn: 0.081 ± 0.073
0.162CysPro: 0.162 ± 0.115
0.162CysGln: 0.162 ± 0.119
0.0CysArg: 0.0 ± 0.0
0.243CysSer: 0.243 ± 0.134
0.162CysThr: 0.162 ± 0.119
0.487CysVal: 0.487 ± 0.202
0.162CysTrp: 0.162 ± 0.114
0.243CysTyr: 0.243 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
3.814AspAla: 3.814 ± 0.532
0.243AspCys: 0.243 ± 0.144
3.895AspAsp: 3.895 ± 0.604
3.651AspGlu: 3.651 ± 0.512
3.976AspPhe: 3.976 ± 0.513
6.41AspGly: 6.41 ± 1.069
0.73AspHis: 0.73 ± 0.235
4.219AspIle: 4.219 ± 0.571
6.329AspLys: 6.329 ± 0.628
4.301AspLeu: 4.301 ± 0.671
1.461AspMet: 1.461 ± 0.296
3.976AspAsn: 3.976 ± 0.561
1.542AspPro: 1.542 ± 0.352
1.217AspGln: 1.217 ± 0.285
2.678AspArg: 2.678 ± 0.475
3.408AspSer: 3.408 ± 0.682
3.733AspThr: 3.733 ± 0.501
3.408AspVal: 3.408 ± 0.543
0.406AspTrp: 0.406 ± 0.191
3.733AspTyr: 3.733 ± 0.614
0.0AspXaa: 0.0 ± 0.0
Glu
4.219GluAla: 4.219 ± 0.531
0.406GluCys: 0.406 ± 0.187
3.57GluAsp: 3.57 ± 0.615
5.761GluGlu: 5.761 ± 0.742
2.272GluPhe: 2.272 ± 0.427
2.272GluGly: 2.272 ± 0.324
1.217GluHis: 1.217 ± 0.279
6.654GluIle: 6.654 ± 0.727
6.41GluLys: 6.41 ± 0.71
7.79GluLeu: 7.79 ± 1.005
1.947GluMet: 1.947 ± 0.406
4.625GluAsn: 4.625 ± 0.511
2.191GluPro: 2.191 ± 0.544
4.787GluGln: 4.787 ± 0.556
3.408GluArg: 3.408 ± 0.552
3.083GluSer: 3.083 ± 0.83
3.814GluThr: 3.814 ± 0.482
4.301GluVal: 4.301 ± 0.565
0.568GluTrp: 0.568 ± 0.201
2.84GluTyr: 2.84 ± 0.455
0.0GluXaa: 0.0 ± 0.0
Phe
2.515PheAla: 2.515 ± 0.493
0.325PheCys: 0.325 ± 0.147
4.625PheAsp: 4.625 ± 0.544
4.463PheGlu: 4.463 ± 0.738
1.704PhePhe: 1.704 ± 0.442
2.353PheGly: 2.353 ± 0.445
0.73PheHis: 0.73 ± 0.242
1.947PheIle: 1.947 ± 0.324
2.191PheLys: 2.191 ± 0.421
2.353PheLeu: 2.353 ± 0.432
0.974PheMet: 0.974 ± 0.336
3.165PheAsn: 3.165 ± 0.423
0.73PhePro: 0.73 ± 0.287
1.785PheGln: 1.785 ± 0.444
1.461PheArg: 1.461 ± 0.316
3.327PheSer: 3.327 ± 0.501
3.002PheThr: 3.002 ± 0.544
2.029PheVal: 2.029 ± 0.381
0.162PheTrp: 0.162 ± 0.121
1.623PheTyr: 1.623 ± 0.409
0.0PheXaa: 0.0 ± 0.0
Gly
4.382GlyAla: 4.382 ± 0.741
0.406GlyCys: 0.406 ± 0.175
2.515GlyAsp: 2.515 ± 0.423
4.219GlyGlu: 4.219 ± 0.587
3.733GlyPhe: 3.733 ± 0.708
4.95GlyGly: 4.95 ± 0.675
1.136GlyHis: 1.136 ± 0.324
4.706GlyIle: 4.706 ± 0.597
5.193GlyLys: 5.193 ± 0.676
5.193GlyLeu: 5.193 ± 0.94
2.11GlyMet: 2.11 ± 0.531
3.814GlyAsn: 3.814 ± 0.674
0.325GlyPro: 0.325 ± 0.163
2.597GlyGln: 2.597 ± 0.497
3.733GlyArg: 3.733 ± 0.694
4.787GlySer: 4.787 ± 0.866
4.382GlyThr: 4.382 ± 0.687
5.355GlyVal: 5.355 ± 0.679
0.893GlyTrp: 0.893 ± 0.46
2.272GlyTyr: 2.272 ± 0.428
0.0GlyXaa: 0.0 ± 0.0
His
1.055HisAla: 1.055 ± 0.271
0.081HisCys: 0.081 ± 0.071
0.893HisAsp: 0.893 ± 0.291
1.217HisGlu: 1.217 ± 0.317
0.649HisPhe: 0.649 ± 0.211
0.811HisGly: 0.811 ± 0.304
0.325HisHis: 0.325 ± 0.145
1.379HisIle: 1.379 ± 0.387
1.055HisLys: 1.055 ± 0.253
1.217HisLeu: 1.217 ± 0.345
0.325HisMet: 0.325 ± 0.16
0.649HisAsn: 0.649 ± 0.219
0.811HisPro: 0.811 ± 0.309
0.487HisGln: 0.487 ± 0.169
0.649HisArg: 0.649 ± 0.197
1.542HisSer: 1.542 ± 0.404
1.055HisThr: 1.055 ± 0.248
0.487HisVal: 0.487 ± 0.235
0.243HisTrp: 0.243 ± 0.149
0.649HisTyr: 0.649 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
5.599IleAla: 5.599 ± 0.682
0.243IleCys: 0.243 ± 0.133
4.544IleAsp: 4.544 ± 0.442
6.573IleGlu: 6.573 ± 0.856
2.11IlePhe: 2.11 ± 0.479
5.112IleGly: 5.112 ± 0.742
1.136IleHis: 1.136 ± 0.223
3.733IleIle: 3.733 ± 0.627
6.735IleLys: 6.735 ± 0.729
3.733IleLeu: 3.733 ± 0.661
1.623IleMet: 1.623 ± 0.387
3.814IleAsn: 3.814 ± 0.351
2.515IlePro: 2.515 ± 0.473
2.272IleGln: 2.272 ± 0.365
2.272IleArg: 2.272 ± 0.343
4.706IleSer: 4.706 ± 0.472
3.489IleThr: 3.489 ± 0.464
4.057IleVal: 4.057 ± 0.551
0.487IleTrp: 0.487 ± 0.202
2.191IleTyr: 2.191 ± 0.411
0.0IleXaa: 0.0 ± 0.0
Lys
6.329LysAla: 6.329 ± 0.909
0.568LysCys: 0.568 ± 0.197
3.733LysAsp: 3.733 ± 0.591
6.005LysGlu: 6.005 ± 0.606
2.434LysPhe: 2.434 ± 0.411
6.573LysGly: 6.573 ± 0.988
1.542LysHis: 1.542 ± 0.388
5.437LysIle: 5.437 ± 0.797
6.816LysLys: 6.816 ± 0.794
6.816LysLeu: 6.816 ± 0.641
1.704LysMet: 1.704 ± 0.39
5.274LysAsn: 5.274 ± 0.532
2.029LysPro: 2.029 ± 0.416
3.327LysGln: 3.327 ± 0.491
3.651LysArg: 3.651 ± 0.612
5.599LysSer: 5.599 ± 0.629
5.355LysThr: 5.355 ± 0.638
5.599LysVal: 5.599 ± 0.55
1.866LysTrp: 1.866 ± 0.403
3.327LysTyr: 3.327 ± 0.513
0.0LysXaa: 0.0 ± 0.0
Leu
6.167LeuAla: 6.167 ± 0.794
0.487LeuCys: 0.487 ± 0.223
5.274LeuAsp: 5.274 ± 0.574
6.573LeuGlu: 6.573 ± 0.871
2.272LeuPhe: 2.272 ± 0.44
4.219LeuGly: 4.219 ± 0.579
1.298LeuHis: 1.298 ± 0.35
3.57LeuIle: 3.57 ± 0.616
7.709LeuLys: 7.709 ± 0.894
5.599LeuLeu: 5.599 ± 0.565
1.217LeuMet: 1.217 ± 0.284
5.599LeuAsn: 5.599 ± 0.701
3.083LeuPro: 3.083 ± 0.454
3.327LeuGln: 3.327 ± 0.757
3.002LeuArg: 3.002 ± 0.54
5.193LeuSer: 5.193 ± 0.62
5.68LeuThr: 5.68 ± 0.864
4.301LeuVal: 4.301 ± 0.732
0.487LeuTrp: 0.487 ± 0.207
2.353LeuTyr: 2.353 ± 0.392
0.0LeuXaa: 0.0 ± 0.0
Met
2.434MetAla: 2.434 ± 0.468
0.162MetCys: 0.162 ± 0.118
1.217MetAsp: 1.217 ± 0.255
1.623MetGlu: 1.623 ± 0.394
0.73MetPhe: 0.73 ± 0.226
0.811MetGly: 0.811 ± 0.261
0.081MetHis: 0.081 ± 0.082
1.704MetIle: 1.704 ± 0.488
2.434MetLys: 2.434 ± 0.366
1.542MetLeu: 1.542 ± 0.316
0.568MetMet: 0.568 ± 0.197
1.217MetAsn: 1.217 ± 0.419
0.73MetPro: 0.73 ± 0.198
1.136MetGln: 1.136 ± 0.236
1.704MetArg: 1.704 ± 0.375
0.893MetSer: 0.893 ± 0.223
2.191MetThr: 2.191 ± 0.399
0.974MetVal: 0.974 ± 0.287
0.162MetTrp: 0.162 ± 0.115
0.649MetTyr: 0.649 ± 0.228
0.0MetXaa: 0.0 ± 0.0
Asn
4.787AsnAla: 4.787 ± 0.562
0.162AsnCys: 0.162 ± 0.107
3.976AsnAsp: 3.976 ± 0.519
4.057AsnGlu: 4.057 ± 0.571
2.597AsnPhe: 2.597 ± 0.527
5.68AsnGly: 5.68 ± 0.807
1.055AsnHis: 1.055 ± 0.265
3.002AsnIle: 3.002 ± 0.558
5.274AsnLys: 5.274 ± 0.625
4.787AsnLeu: 4.787 ± 0.76
1.785AsnMet: 1.785 ± 0.406
4.544AsnAsn: 4.544 ± 0.774
2.029AsnPro: 2.029 ± 0.512
2.759AsnGln: 2.759 ± 0.469
2.029AsnArg: 2.029 ± 0.508
4.057AsnSer: 4.057 ± 0.71
2.759AsnThr: 2.759 ± 0.389
2.759AsnVal: 2.759 ± 0.478
0.73AsnTrp: 0.73 ± 0.253
1.623AsnTyr: 1.623 ± 0.308
0.0AsnXaa: 0.0 ± 0.0
Pro
1.866ProAla: 1.866 ± 0.348
0.162ProCys: 0.162 ± 0.119
2.029ProAsp: 2.029 ± 0.54
1.623ProGlu: 1.623 ± 0.356
1.298ProPhe: 1.298 ± 0.373
1.217ProGly: 1.217 ± 0.424
0.162ProHis: 0.162 ± 0.148
2.029ProIle: 2.029 ± 0.4
2.597ProLys: 2.597 ± 0.567
1.542ProLeu: 1.542 ± 0.373
0.325ProMet: 0.325 ± 0.202
2.029ProAsn: 2.029 ± 0.416
0.325ProPro: 0.325 ± 0.161
1.461ProGln: 1.461 ± 0.34
1.055ProArg: 1.055 ± 0.296
1.785ProSer: 1.785 ± 0.284
1.947ProThr: 1.947 ± 0.391
2.029ProVal: 2.029 ± 0.377
0.325ProTrp: 0.325 ± 0.181
1.623ProTyr: 1.623 ± 0.427
0.0ProXaa: 0.0 ± 0.0
Gln
3.489GlnAla: 3.489 ± 0.51
0.081GlnCys: 0.081 ± 0.082
1.461GlnAsp: 1.461 ± 0.308
3.327GlnGlu: 3.327 ± 0.549
1.542GlnPhe: 1.542 ± 0.389
3.408GlnGly: 3.408 ± 0.782
0.487GlnHis: 0.487 ± 0.201
2.84GlnIle: 2.84 ± 0.337
3.651GlnLys: 3.651 ± 0.599
3.246GlnLeu: 3.246 ± 0.391
0.893GlnMet: 0.893 ± 0.321
2.029GlnAsn: 2.029 ± 0.436
1.379GlnPro: 1.379 ± 0.35
2.515GlnGln: 2.515 ± 0.653
2.272GlnArg: 2.272 ± 0.408
2.597GlnSer: 2.597 ± 0.509
1.866GlnThr: 1.866 ± 0.461
3.408GlnVal: 3.408 ± 0.614
0.325GlnTrp: 0.325 ± 0.147
2.029GlnTyr: 2.029 ± 0.381
0.0GlnXaa: 0.0 ± 0.0
Arg
3.327ArgAla: 3.327 ± 0.518
0.081ArgCys: 0.081 ± 0.101
2.434ArgAsp: 2.434 ± 0.396
2.191ArgGlu: 2.191 ± 0.482
2.272ArgPhe: 2.272 ± 0.353
3.083ArgGly: 3.083 ± 0.556
0.649ArgHis: 0.649 ± 0.196
3.002ArgIle: 3.002 ± 0.458
3.327ArgLys: 3.327 ± 0.465
3.976ArgLeu: 3.976 ± 0.55
0.974ArgMet: 0.974 ± 0.237
2.434ArgAsn: 2.434 ± 0.484
1.785ArgPro: 1.785 ± 0.471
1.866ArgGln: 1.866 ± 0.326
1.866ArgArg: 1.866 ± 0.351
1.461ArgSer: 1.461 ± 0.294
2.597ArgThr: 2.597 ± 0.398
2.353ArgVal: 2.353 ± 0.38
0.893ArgTrp: 0.893 ± 0.313
2.191ArgTyr: 2.191 ± 0.402
0.0ArgXaa: 0.0 ± 0.0
Ser
4.625SerAla: 4.625 ± 0.613
0.162SerCys: 0.162 ± 0.099
4.463SerAsp: 4.463 ± 0.563
3.895SerGlu: 3.895 ± 0.63
2.84SerPhe: 2.84 ± 0.562
3.733SerGly: 3.733 ± 0.84
1.379SerHis: 1.379 ± 0.442
3.57SerIle: 3.57 ± 0.523
3.246SerLys: 3.246 ± 0.528
4.706SerLeu: 4.706 ± 0.544
1.704SerMet: 1.704 ± 0.302
3.57SerAsn: 3.57 ± 0.408
1.379SerPro: 1.379 ± 0.365
3.083SerGln: 3.083 ± 0.542
2.11SerArg: 2.11 ± 0.317
4.219SerSer: 4.219 ± 0.912
5.599SerThr: 5.599 ± 0.7
4.625SerVal: 4.625 ± 0.75
0.73SerTrp: 0.73 ± 0.184
1.785SerTyr: 1.785 ± 0.311
0.0SerXaa: 0.0 ± 0.0
Thr
3.489ThrAla: 3.489 ± 1.236
0.243ThrCys: 0.243 ± 0.128
5.112ThrAsp: 5.112 ± 0.77
3.976ThrGlu: 3.976 ± 0.477
2.353ThrPhe: 2.353 ± 0.391
4.95ThrGly: 4.95 ± 0.644
0.73ThrHis: 0.73 ± 0.311
5.923ThrIle: 5.923 ± 0.58
5.031ThrLys: 5.031 ± 0.525
6.005ThrLeu: 6.005 ± 0.707
1.217ThrMet: 1.217 ± 0.376
3.814ThrAsn: 3.814 ± 0.551
2.272ThrPro: 2.272 ± 0.42
2.353ThrGln: 2.353 ± 0.496
2.515ThrArg: 2.515 ± 0.443
2.759ThrSer: 2.759 ± 0.408
4.869ThrThr: 4.869 ± 0.701
5.031ThrVal: 5.031 ± 0.706
1.217ThrTrp: 1.217 ± 0.312
1.866ThrTyr: 1.866 ± 0.298
0.0ThrXaa: 0.0 ± 0.0
Val
4.869ValAla: 4.869 ± 0.576
0.487ValCys: 0.487 ± 0.173
4.544ValAsp: 4.544 ± 0.552
3.976ValGlu: 3.976 ± 0.584
2.434ValPhe: 2.434 ± 0.397
3.976ValGly: 3.976 ± 0.518
1.217ValHis: 1.217 ± 0.306
4.787ValIle: 4.787 ± 0.612
5.274ValLys: 5.274 ± 0.678
3.327ValLeu: 3.327 ± 0.504
1.055ValMet: 1.055 ± 0.25
3.408ValAsn: 3.408 ± 0.48
0.811ValPro: 0.811 ± 0.236
2.515ValGln: 2.515 ± 0.411
2.272ValArg: 2.272 ± 0.39
4.382ValSer: 4.382 ± 0.564
5.599ValThr: 5.599 ± 0.53
3.976ValVal: 3.976 ± 0.685
0.73ValTrp: 0.73 ± 0.301
2.597ValTyr: 2.597 ± 0.549
0.0ValXaa: 0.0 ± 0.0
Trp
1.298TrpAla: 1.298 ± 0.315
0.0TrpCys: 0.0 ± 0.0
0.406TrpAsp: 0.406 ± 0.151
0.811TrpGlu: 0.811 ± 0.222
0.406TrpPhe: 0.406 ± 0.189
0.406TrpGly: 0.406 ± 0.161
0.243TrpHis: 0.243 ± 0.129
0.568TrpIle: 0.568 ± 0.213
1.217TrpLys: 1.217 ± 0.288
0.974TrpLeu: 0.974 ± 0.311
0.162TrpMet: 0.162 ± 0.135
0.568TrpAsn: 0.568 ± 0.157
0.162TrpPro: 0.162 ± 0.171
0.811TrpGln: 0.811 ± 0.251
0.568TrpArg: 0.568 ± 0.209
0.325TrpSer: 0.325 ± 0.148
0.73TrpThr: 0.73 ± 0.327
0.649TrpVal: 0.649 ± 0.233
0.325TrpTrp: 0.325 ± 0.144
1.136TrpTyr: 1.136 ± 0.572
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.947TyrAla: 1.947 ± 0.349
0.406TyrCys: 0.406 ± 0.228
2.597TyrAsp: 2.597 ± 0.426
1.785TyrGlu: 1.785 ± 0.345
2.353TyrPhe: 2.353 ± 0.511
2.597TyrGly: 2.597 ± 0.443
0.649TyrHis: 0.649 ± 0.257
2.434TyrIle: 2.434 ± 0.528
3.489TyrLys: 3.489 ± 0.593
3.895TyrLeu: 3.895 ± 0.659
0.893TyrMet: 0.893 ± 0.25
2.11TyrAsn: 2.11 ± 0.387
1.298TyrPro: 1.298 ± 0.342
1.542TyrGln: 1.542 ± 0.284
2.353TyrArg: 2.353 ± 0.466
2.597TyrSer: 2.597 ± 0.442
2.434TyrThr: 2.434 ± 0.548
1.704TyrVal: 1.704 ± 0.399
0.325TyrTrp: 0.325 ± 0.149
1.866TyrTyr: 1.866 ± 0.572
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12325 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski