Leucobacter sp. HDW9A

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2376 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8FPR9|A0A6G8FPR9_9MICO DUF2200 domain-containing protein OS=Leucobacter sp. HDW9A OX=2714933 GN=G7066_06465 PE=4 SV=1
MM1 pKa = 6.93TAVLVDD7 pKa = 3.48EE8 pKa = 5.37FGNAVSGEE16 pKa = 4.14EE17 pKa = 3.94TALLATTADD26 pKa = 3.9GLGSGGITAFTEE38 pKa = 4.52SVVPGTYY45 pKa = 8.78TAAVTSSVAGNKK57 pKa = 8.25TLAVALSGEE66 pKa = 4.29PVLPAGNTIARR77 pKa = 11.84FVAGGVDD84 pKa = 3.32TGNPGTTYY92 pKa = 10.72AVSGGQQTVGTGAHH106 pKa = 7.07TITVTLVDD114 pKa = 3.66AEE116 pKa = 4.59GNPVPAQAAGISADD130 pKa = 3.64TGDD133 pKa = 4.37DD134 pKa = 3.9LGDD137 pKa = 3.65GSISDD142 pKa = 3.81FTEE145 pKa = 3.74TGTAGTYY152 pKa = 6.87TASVTSTVSGPKK164 pKa = 9.66TITVLYY170 pKa = 9.17GANPVTLAGNDD181 pKa = 3.26TALFVPGAIDD191 pKa = 3.43FTNANTNYY199 pKa = 9.14TVSTGTRR206 pKa = 11.84PVGAGEE212 pKa = 4.09HH213 pKa = 5.16TVTVILSDD221 pKa = 3.46EE222 pKa = 4.43FGNPVSGQQAAIVPATADD240 pKa = 3.25ALGTGEE246 pKa = 4.76FSAFTEE252 pKa = 4.26TATAGTYY259 pKa = 7.66EE260 pKa = 4.11ATVTSSVSGVKK271 pKa = 10.38AITVSVGVNSVRR283 pKa = 11.84LSGNGTAAFVSGDD296 pKa = 3.14VDD298 pKa = 4.7LEE300 pKa = 4.14NSEE303 pKa = 4.26TNYY306 pKa = 10.19VVTTGDD312 pKa = 3.24VSVDD316 pKa = 3.49GGSHH320 pKa = 4.95SVIVTLADD328 pKa = 3.49EE329 pKa = 4.93LNNPVTGQSADD340 pKa = 3.81LLASTINSLGSGTITGFTEE359 pKa = 3.85TAIPGEE365 pKa = 4.21YY366 pKa = 9.63QATITSSIAGNKK378 pKa = 9.89DD379 pKa = 2.69ITVSLGGEE387 pKa = 4.04PVTAAGNTAASFVAGGVDD405 pKa = 3.39VGNSGTRR412 pKa = 11.84YY413 pKa = 9.67SVSAGNQTVSTGSHH427 pKa = 4.85TVTVTLADD435 pKa = 3.89AEE437 pKa = 4.65DD438 pKa = 3.9NAVSGQAAGLSATTAADD455 pKa = 3.68LGTGEE460 pKa = 4.7IGTFTEE466 pKa = 4.25TATAGTYY473 pKa = 9.1QATITSTVSGPKK485 pKa = 9.9PITVTYY491 pKa = 9.97GGSAVTASGNTTAVFVAGEE510 pKa = 3.93VDD512 pKa = 4.28LNNGSTRR519 pKa = 11.84YY520 pKa = 10.03SVTTGDD526 pKa = 3.46QVVGSGQHH534 pKa = 4.88TVTVTLRR541 pKa = 11.84DD542 pKa = 3.64EE543 pKa = 4.76FNNPVTNQASNLVADD558 pKa = 4.44SAQNLGVGTISPFTEE573 pKa = 4.24TSDD576 pKa = 3.64GVYY579 pKa = 9.78EE580 pKa = 4.44ASLSSTVSGSKK591 pKa = 10.57NITVALGLASVPLSGNGVASFVAGSVDD618 pKa = 4.11LNDD621 pKa = 3.56SGTVYY626 pKa = 10.65SVSTGEE632 pKa = 4.09QQVGTGLHH640 pKa = 6.62TITVTLLDD648 pKa = 3.6VFGNGVPGQQALIDD662 pKa = 4.39ANTSAVIGTGSFSAFAPTATPGIYY686 pKa = 9.0TATVSSSISGSKK698 pKa = 10.12PIDD701 pKa = 3.41VSFEE705 pKa = 3.79GAAVDD710 pKa = 4.57ASGNTDD716 pKa = 3.21ALFAAGGVDD725 pKa = 3.53PANPASNYY733 pKa = 10.03AVSTGDD739 pKa = 3.72EE740 pKa = 4.08IVGTGSHH747 pKa = 5.46TVTVALADD755 pKa = 3.67ALGNPVPGQAAQLAATTSGDD775 pKa = 3.46LGTGAFTGFVEE786 pKa = 4.57SASVGIYY793 pKa = 8.59EE794 pKa = 4.43ATMTSTVSGSKK805 pKa = 10.14PVTVTYY811 pKa = 10.92NSAPITLAGNGSASFISGDD830 pKa = 3.49TDD832 pKa = 3.52LTSPATSYY840 pKa = 11.12SVSSGNASVEE850 pKa = 4.43GGSHH854 pKa = 7.19LVTITLADD862 pKa = 3.64SFANPVPGKK871 pKa = 10.43AVDD874 pKa = 4.44LVASTTDD881 pKa = 3.53DD882 pKa = 4.16LGSGTITAVTEE893 pKa = 4.24SLTTPGTYY901 pKa = 9.65QATVTSSVAGGKK913 pKa = 10.07AIAVALGGDD922 pKa = 3.61SVTLNGNGTAQFIAGGPDD940 pKa = 3.14TDD942 pKa = 3.59NAGTVFEE949 pKa = 4.52VTEE952 pKa = 4.65GPQTVGAGSHH962 pKa = 4.53TVRR965 pKa = 11.84VNLRR969 pKa = 11.84DD970 pKa = 3.42SSGNPVSGEE979 pKa = 3.68AAGINANTVDD989 pKa = 3.85GLGSGTISSFTEE1001 pKa = 3.77TGTPGLYY1008 pKa = 9.27EE1009 pKa = 4.23ASVTSTLIGGKK1020 pKa = 9.96SITVNYY1026 pKa = 8.47GASAITAQGNTVALFVAAAVDD1047 pKa = 3.86PGSPGTNYY1055 pKa = 10.61SVTSGNQQVGTGQHH1069 pKa = 5.09TVTVTLADD1077 pKa = 3.28AFGNRR1082 pKa = 11.84VSGQSALLDD1091 pKa = 3.77AATTDD1096 pKa = 3.67SLGSGTIGGFVEE1108 pKa = 4.42TGTTGVYY1115 pKa = 9.84LAPVTSTLAGSKK1127 pKa = 10.4AVTASYY1133 pKa = 11.26NSLPITLSGNGNAVFVAGGVDD1154 pKa = 3.35VGNAATSYY1162 pKa = 9.42TVSSGDD1168 pKa = 3.36ASVSSGSHH1176 pKa = 6.57TITMTLADD1184 pKa = 3.86AFGNPVPGMAPRR1196 pKa = 11.84VTATTAANLGSGTITGVTEE1215 pKa = 3.63QATPGTYY1222 pKa = 9.68QATVTSSIAGNKK1234 pKa = 9.97DD1235 pKa = 2.45ITALFDD1241 pKa = 3.98AAPLTLSGNGIARR1254 pKa = 11.84FVAGEE1259 pKa = 4.73LILQTRR1265 pKa = 11.84APLSPP1270 pKa = 3.86

Molecular weight: 123.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8FMM7|A0A6G8FMM7_9MICO 3-hydroxyacyl-CoA dehydrogenase family protein OS=Leucobacter sp. HDW9A OX=2714933 GN=G7066_01125 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.45GRR40 pKa = 11.84SKK42 pKa = 10.13LTAA45 pKa = 4.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2376

0

2376

718273

29

2824

302.3

32.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.096 ± 0.068

0.62 ± 0.014

5.518 ± 0.043

6.009 ± 0.062

3.317 ± 0.031

8.686 ± 0.055

1.845 ± 0.026

4.833 ± 0.038

2.778 ± 0.04

10.013 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.928 ± 0.021

2.625 ± 0.035

5.076 ± 0.033

3.242 ± 0.029

6.46 ± 0.074

6.46 ± 0.045

6.457 ± 0.078

8.631 ± 0.06

1.377 ± 0.021

2.029 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski