Flavobacteriaceae bacterium MAR_2010_188

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6FBX0|A0A1G6FBX0_9FLAO Gliding motility-associated C-terminal domain-containing protein OS=Flavobacteriaceae bacterium MAR_2010_188 OX=1250295 GN=SAMN03097699_3343 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.47NIIKK6 pKa = 10.32GLSILSAVLVMVSCDD21 pKa = 3.44TDD23 pKa = 3.7PDD25 pKa = 3.54NTIYY29 pKa = 10.79EE30 pKa = 4.22VLDD33 pKa = 3.89FEE35 pKa = 5.1KK36 pKa = 10.93GAVLRR41 pKa = 11.84TVSIEE46 pKa = 3.92NNLLNSSNPSSTFSVTVEE64 pKa = 4.11EE65 pKa = 4.23QDD67 pKa = 4.01KK68 pKa = 11.24EE69 pKa = 4.32DD70 pKa = 3.75GGLMEE75 pKa = 5.05SVDD78 pKa = 3.51VYY80 pKa = 11.24VRR82 pKa = 11.84YY83 pKa = 9.79RR84 pKa = 11.84DD85 pKa = 3.55NTPADD90 pKa = 4.64GINEE94 pKa = 4.2SSTAFIKK101 pKa = 10.32SIPASDD107 pKa = 3.68FTVGPVGLPRR117 pKa = 11.84ATITATYY124 pKa = 10.57GEE126 pKa = 4.23ATAALGFGNEE136 pKa = 4.41TISAGDD142 pKa = 3.32ILVFEE147 pKa = 4.94LVLNLTDD154 pKa = 3.74GRR156 pKa = 11.84SFGPEE161 pKa = 3.49SSNSVLTGIFFKK173 pKa = 11.16APFSYY178 pKa = 9.15NTLITCSPQPGDD190 pKa = 3.63YY191 pKa = 9.4VVKK194 pKa = 10.46MFDD197 pKa = 3.29SYY199 pKa = 11.98GDD201 pKa = 3.24GWQTDD206 pKa = 3.83DD207 pKa = 4.16GNGGSGLMIDD217 pKa = 4.23LDD219 pKa = 4.04GTVVEE224 pKa = 5.27VGLCSPYY231 pKa = 10.36LASDD235 pKa = 4.0FDD237 pKa = 4.09CTEE240 pKa = 4.17GVSEE244 pKa = 4.87GEE246 pKa = 4.27VTVTIPEE253 pKa = 4.37GTEE256 pKa = 3.83SASWTFPGDD265 pKa = 3.15QYY267 pKa = 11.86GEE269 pKa = 3.9IRR271 pKa = 11.84FEE273 pKa = 4.3VYY275 pKa = 10.6APDD278 pKa = 3.51GTLVFDD284 pKa = 4.29SGDD287 pKa = 3.44FGEE290 pKa = 4.76QGAGLLPIVVCAEE303 pKa = 3.83

Molecular weight: 32.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6F5H3|A0A1G6F5H3_9FLAO Glycosyltransferase family 10 (Fucosyltransferase) C-term OS=Flavobacteriaceae bacterium MAR_2010_188 OX=1250295 GN=SAMN03097699_3024 PE=3 SV=1
MM1 pKa = 7.84PSGKK5 pKa = 9.32KK6 pKa = 9.59RR7 pKa = 11.84KK8 pKa = 7.05RR9 pKa = 11.84HH10 pKa = 5.11KK11 pKa = 10.59VATHH15 pKa = 5.21KK16 pKa = 10.3RR17 pKa = 11.84KK18 pKa = 9.62KK19 pKa = 9.04RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84ANRR25 pKa = 11.84HH26 pKa = 4.77KK27 pKa = 10.58KK28 pKa = 9.8KK29 pKa = 10.51KK30 pKa = 10.04

Molecular weight: 3.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3255

0

3255

1129829

29

5004

347.1

39.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.179 ± 0.054

0.755 ± 0.021

6.054 ± 0.044

6.751 ± 0.043

5.148 ± 0.042

6.448 ± 0.043

1.672 ± 0.022

8.053 ± 0.05

7.443 ± 0.065

9.174 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.025

6.142 ± 0.044

3.401 ± 0.029

3.243 ± 0.027

3.543 ± 0.036

6.87 ± 0.034

5.69 ± 0.116

6.103 ± 0.039

1.015 ± 0.014

4.031 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski