Leuconostoc phage LN25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mccleskeyvirinae; Unaquatrovirus; Leuconostoc virus LN25

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A059PAI2|A0A059PAI2_9CAUD Putative phage-related protein OS=Leuconostoc phage LN25 OX=1262518 GN=phiLN25_03 PE=4 SV=1
MM1 pKa = 7.62IFDD4 pKa = 4.07YY5 pKa = 11.23KK6 pKa = 10.85EE7 pKa = 3.6ILQLDD12 pKa = 4.2DD13 pKa = 3.6EE14 pKa = 5.66SYY16 pKa = 10.84IAWTAHH22 pKa = 6.39LEE24 pKa = 4.07MLEE27 pKa = 4.16KK28 pKa = 10.41QALTQAEE35 pKa = 4.9LINPNVQKK43 pKa = 10.78DD44 pKa = 3.81VSNNTLGSYY53 pKa = 9.58CQEE56 pKa = 3.81FVFMAEE62 pKa = 4.13YY63 pKa = 10.69GSDD66 pKa = 3.69LSASMYY72 pKa = 10.05KK73 pKa = 8.99WHH75 pKa = 7.08TSNIATLEE83 pKa = 3.81EE84 pKa = 4.62AIRR87 pKa = 11.84YY88 pKa = 8.65AGG90 pKa = 3.33

Molecular weight: 10.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A059PB18|A0A059PB18_9CAUD Uncharacterized protein OS=Leuconostoc phage LN25 OX=1262518 GN=phiLN25_025 PE=4 SV=1
MM1 pKa = 7.58SARR4 pKa = 11.84KK5 pKa = 9.14IDD7 pKa = 3.63ISDD10 pKa = 3.73LTKK13 pKa = 10.81LPPRR17 pKa = 11.84LMTAKK22 pKa = 10.01ILEE25 pKa = 4.4KK26 pKa = 9.78RR27 pKa = 11.84DD28 pKa = 3.59EE29 pKa = 4.25VAKK32 pKa = 10.68QAGSDD37 pKa = 3.46MTSALSVWRR46 pKa = 11.84STAPIKK52 pKa = 8.94TGRR55 pKa = 11.84MRR57 pKa = 11.84STMTLNRR64 pKa = 11.84GKK66 pKa = 10.68YY67 pKa = 9.32GATLVPRR74 pKa = 11.84DD75 pKa = 3.54SYY77 pKa = 10.78MKK79 pKa = 10.56VVNAVNKK86 pKa = 9.86RR87 pKa = 11.84GKK89 pKa = 9.44HH90 pKa = 4.14QGFLDD95 pKa = 3.59RR96 pKa = 11.84FKK98 pKa = 10.86RR99 pKa = 11.84GQGKK103 pKa = 9.58SFLQKK108 pKa = 9.19WW109 pKa = 3.4

Molecular weight: 12.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

8595

48

855

209.6

23.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.143 ± 0.663

0.291 ± 0.093

6.562 ± 0.371

6.108 ± 0.587

4.607 ± 0.207

7.004 ± 0.692

1.431 ± 0.214

7.318 ± 0.296

7.667 ± 0.44

8.074 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.212

6.713 ± 0.3

2.792 ± 0.198

3.374 ± 0.255

3.455 ± 0.311

6.806 ± 0.634

7.004 ± 0.417

6.504 ± 0.292

1.07 ± 0.122

4.305 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski