Beihai picorna-like virus 7
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KHJ6|A0A1L3KHJ6_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 7 OX=1922616 PE=4 SV=1
MM1 pKa = 7.68 PVLDD5 pKa = 5.6 SIPEE9 pKa = 4.04 NDD11 pKa = 5.7 DD12 pKa = 3.62 VLQCDD17 pKa = 3.44 TRR19 pKa = 11.84 LIKK22 pKa = 10.55 PEE24 pKa = 4.25 LFRR27 pKa = 11.84 CATSGFTPQSGEE39 pKa = 3.51 VDD41 pKa = 3.43 QTVTQAPEE49 pKa = 4.06 GTVEE53 pKa = 4.28 QIVAFDD59 pKa = 3.79 DD60 pKa = 5.05 QEE62 pKa = 4.89 AGWMKK67 pKa = 10.57 DD68 pKa = 2.72 ASGQFDD74 pKa = 3.57 STMDD78 pKa = 3.9 LVDD81 pKa = 5.7 KK82 pKa = 11.21 SDD84 pKa = 3.58 TDD86 pKa = 3.59 LGAFLGRR93 pKa = 11.84 PVKK96 pKa = 10.15 IDD98 pKa = 3.17 TQAWVVGQPLYY109 pKa = 10.79 KK110 pKa = 10.37 QINPWEE116 pKa = 4.19 LFVTHH121 pKa = 7.16 PAMANKK127 pKa = 9.49 LAHH130 pKa = 6.09 YY131 pKa = 8.65 EE132 pKa = 3.91 LLRR135 pKa = 11.84 GNLNVKK141 pKa = 9.33 VVISGTGFHH150 pKa = 6.78 YY151 pKa = 10.7 GRR153 pKa = 11.84 TLVSYY158 pKa = 10.89 NPLNGYY164 pKa = 10.17 DD165 pKa = 3.64 EE166 pKa = 5.71 ASVTRR171 pKa = 11.84 NFIDD175 pKa = 3.21 QDD177 pKa = 4.26 LIAASQRR184 pKa = 11.84 PCIFLNPTDD193 pKa = 3.78 NSGGQMKK200 pKa = 10.7 LPFFFLQNYY209 pKa = 6.72 MSLSKK214 pKa = 10.93 GDD216 pKa = 3.68 YY217 pKa = 10.16 RR218 pKa = 11.84 DD219 pKa = 3.69 MGVLDD224 pKa = 3.94 YY225 pKa = 11.4 KK226 pKa = 11.39 SFGNLLHH233 pKa = 7.16 ANGGDD238 pKa = 3.41 DD239 pKa = 3.46 PVYY242 pKa = 8.81 ITTYY246 pKa = 10.61 AWMTEE251 pKa = 4.12 TSLTMPTSITSTITLQNEE269 pKa = 4.39 TVDD272 pKa = 3.67 PDD274 pKa = 4.04 VFPLGPPYY282 pKa = 10.79 GVGNGAEE289 pKa = 4.24 NGVGPPFSPQSGFQPQAGGKK309 pKa = 9.46 PNMKK313 pKa = 9.92 KK314 pKa = 10.47 SNKK317 pKa = 9.38 LQSKK321 pKa = 9.87 GNARR325 pKa = 11.84 GKK327 pKa = 10.69 AKK329 pKa = 10.76 LNTGDD334 pKa = 4.34 EE335 pKa = 4.37 YY336 pKa = 11.47 GQGIISAPASAVAKK350 pKa = 9.79 AAGMLTSIPFIEE362 pKa = 5.27 PYY364 pKa = 10.83 ARR366 pKa = 11.84 ATQMVASKK374 pKa = 9.83 VGQVASIFGYY384 pKa = 9.93 SRR386 pKa = 11.84 PAVVSDD392 pKa = 4.31 FLLQKK397 pKa = 10.27 PSPTGNFANVDD408 pKa = 2.99 ASDD411 pKa = 3.4 AVNRR415 pKa = 11.84 LTLDD419 pKa = 3.34 SKK421 pKa = 11.18 QEE423 pKa = 3.6 ITIDD427 pKa = 3.46 SRR429 pKa = 11.84 TVGLDD434 pKa = 3.14 GGDD437 pKa = 3.23 QMAFDD442 pKa = 5.29 NIQTRR447 pKa = 11.84 EE448 pKa = 4.0 SYY450 pKa = 9.07 LTSFTMSSTDD460 pKa = 3.43 APDD463 pKa = 4.79 AILWNSYY470 pKa = 7.16 VTPNLFGINNPEE482 pKa = 3.75 LHH484 pKa = 5.63 MTPMAMLAQYY494 pKa = 9.79 FSAWQGTIKK503 pKa = 10.4 FRR505 pKa = 11.84 FQIVKK510 pKa = 10.78 SNFHH514 pKa = 6.83 KK515 pKa = 10.83 GRR517 pKa = 11.84 ILVRR521 pKa = 11.84 YY522 pKa = 8.91 DD523 pKa = 3.43 PRR525 pKa = 11.84 SHH527 pKa = 7.29 SSDD530 pKa = 3.08 VNYY533 pKa = 6.9 TTNYY537 pKa = 9.87 SRR539 pKa = 11.84 VIDD542 pKa = 4.62 LATEE546 pKa = 4.1 DD547 pKa = 4.3 DD548 pKa = 4.39 FEE550 pKa = 6.08 IEE552 pKa = 4.36 IGWGQNVPFLGNQQANVSRR571 pKa = 11.84 VYY573 pKa = 11.02 YY574 pKa = 10.51 GGTRR578 pKa = 11.84 LTTDD582 pKa = 2.57 SGGNHH587 pKa = 6.38 NGVIEE592 pKa = 4.32 VNVVNSLVAPAADD605 pKa = 3.22 SDD607 pKa = 3.4 IRR609 pKa = 11.84 INVFVSAGDD618 pKa = 3.53 DD619 pKa = 3.88 MKK621 pKa = 10.47 WGRR624 pKa = 11.84 PNPDD628 pKa = 3.45 KK629 pKa = 10.82 IRR631 pKa = 11.84 NLHH634 pKa = 5.83 YY635 pKa = 10.45 FPATAPPLNEE645 pKa = 4.21 FQPQSGMGGDD655 pKa = 4.13 LSGTTGDD662 pKa = 3.9 SATDD666 pKa = 3.36 RR667 pKa = 11.84 PTEE670 pKa = 4.04 ADD672 pKa = 3.21 NVPSVGCPADD682 pKa = 4.64 DD683 pKa = 3.79 DD684 pKa = 5.15 HH685 pKa = 8.17 MMDD688 pKa = 3.32 VFFGEE693 pKa = 4.46 MPVSLRR699 pKa = 11.84 EE700 pKa = 3.47 IFRR703 pKa = 11.84 RR704 pKa = 11.84 YY705 pKa = 9.36 VFEE708 pKa = 4.65 KK709 pKa = 10.48 YY710 pKa = 9.6 FVPTAPTADD719 pKa = 3.62 GALTTSNYY727 pKa = 9.76 RR728 pKa = 11.84 IKK730 pKa = 10.73 SFPFHH735 pKa = 7.46 SGDD738 pKa = 4.51 DD739 pKa = 3.84 PDD741 pKa = 5.26 GLDD744 pKa = 3.42 VKK746 pKa = 11.24 SDD748 pKa = 3.31 GLRR751 pKa = 11.84 YY752 pKa = 9.83 NQVQTSPIAFFAPCFAGWRR771 pKa = 11.84 GSLRR775 pKa = 11.84 HH776 pKa = 5.65 KK777 pKa = 9.7 WVFSGEE783 pKa = 4.3 SLQSPLISNYY793 pKa = 10.05 GFIAANGAQSTEE805 pKa = 3.8 WTVTNDD811 pKa = 3.19 LEE813 pKa = 4.58 AEE815 pKa = 4.28 KK816 pKa = 10.44 FLSATFGKK824 pKa = 10.24 FSNGGSAGTNLGVNNTIEE842 pKa = 4.29 TEE844 pKa = 3.89 MPYY847 pKa = 11.02 YY848 pKa = 10.23 NGNRR852 pKa = 11.84 FSSSRR857 pKa = 11.84 ILSADD862 pKa = 3.33 NNSSYY867 pKa = 11.04 SYY869 pKa = 10.26 QVEE872 pKa = 4.87 TIQSKK877 pKa = 9.68 SVPSGPVPSVVTRR890 pKa = 11.84 DD891 pKa = 2.88 AFYY894 pKa = 11.03 RR895 pKa = 11.84 MCAAGEE901 pKa = 4.07 DD902 pKa = 3.81 FTLFFWTGVPIMYY915 pKa = 9.9 NYY917 pKa = 10.35 SRR919 pKa = 11.84 TYY921 pKa = 9.9 TSS923 pKa = 2.9
Molecular weight: 101.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.732
IPC2_protein 4.762
IPC_protein 4.749
Toseland 4.596
ProMoST 4.889
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.635
Rodwell 4.596
Grimsley 4.507
Solomon 4.724
Lehninger 4.685
Nozaki 4.838
DTASelect 5.067
Thurlkill 4.609
EMBOSS 4.66
Sillero 4.889
Patrickios 3.808
IPC_peptide 4.736
IPC2_peptide 4.876
IPC2.peptide.svr19 4.852
Protein with the highest isoelectric point:
>tr|A0A1L3KHM6|A0A1L3KHM6_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 7 OX=1922616 PE=4 SV=1
MM1 pKa = 7.09 GQSPCTWANRR11 pKa = 11.84 PNKK14 pKa = 8.87 TLYY17 pKa = 10.62 FVMFQINSKK26 pKa = 8.97 TKK28 pKa = 10.52 LDD30 pKa = 3.53 VSSARR35 pKa = 11.84 GRR37 pKa = 11.84 KK38 pKa = 8.2 SKK40 pKa = 10.39 SYY42 pKa = 10.6 RR43 pKa = 11.84 GTLQGLRR50 pKa = 11.84 IQQCLFVGAGWFLTSYY66 pKa = 10.01 MPIRR70 pKa = 11.84 EE71 pKa = 4.29 CTTLSFEE78 pKa = 4.09 GLSYY82 pKa = 11.0 DD83 pKa = 3.45 FQLFVPLFRR92 pKa = 11.84 PTCDD96 pKa = 3.01 FVVALYY102 pKa = 10.78 VEE104 pKa = 4.56 EE105 pKa = 4.42 LADD108 pKa = 3.7 VQSKK112 pKa = 9.22 VVLQSDD118 pKa = 4.06 APDD121 pKa = 3.27 SFQYY125 pKa = 10.9 LFSGKK130 pKa = 8.44 SHH132 pKa = 7.06 SLTKK136 pKa = 9.76 MVPYY140 pKa = 9.84 YY141 pKa = 11.15 GKK143 pKa = 10.3 GQRR146 pKa = 11.84 ADD148 pKa = 3.32 IIKK151 pKa = 9.66 CDD153 pKa = 3.72 EE154 pKa = 3.91 MALVYY159 pKa = 10.31 GINKK163 pKa = 9.73 YY164 pKa = 10.07 ILPKK168 pKa = 10.41 FSFFRR173 pKa = 11.84 FKK175 pKa = 10.03 THH177 pKa = 5.98 LCEE180 pKa = 4.1 VCKK183 pKa = 11.01 SNVNGRR189 pKa = 11.84 VTYY192 pKa = 10.44 GVLAYY197 pKa = 10.49 HH198 pKa = 6.82 KK199 pKa = 8.47 TQDD202 pKa = 2.94 WKK204 pKa = 11.02 RR205 pKa = 11.84 GNKK208 pKa = 9.87 LGGLRR213 pKa = 11.84 VYY215 pKa = 9.38 TQGTLSATYY224 pKa = 10.26 KK225 pKa = 10.55 RR226 pKa = 11.84 LRR228 pKa = 3.69
Molecular weight: 26.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.859
IPC2_protein 9.165
IPC_protein 9.092
Toseland 9.589
ProMoST 9.502
Dawson 9.897
Bjellqvist 9.663
Wikipedia 10.101
Rodwell 10.16
Grimsley 9.999
Solomon 9.926
Lehninger 9.882
Nozaki 9.75
DTASelect 9.619
Thurlkill 9.736
EMBOSS 10.043
Sillero 9.853
Patrickios 4.673
IPC_peptide 9.926
IPC2_peptide 8.536
IPC2.peptide.svr19 8.042
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2764
228
1613
921.3
103.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.368 ± 0.549
1.23 ± 0.477
6.766 ± 0.672
4.522 ± 0.681
6.006 ± 0.205
6.766 ± 0.931
1.7 ± 0.235
5.174 ± 0.686
5.644 ± 1.046
8.068 ± 0.864
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.075 ± 0.309
4.957 ± 0.672
4.848 ± 0.758
3.654 ± 0.567
4.667 ± 0.429
8.466 ± 0.153
5.861 ± 0.743
6.838 ± 0.15
1.447 ± 0.085
3.944 ± 0.532
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here