Tychonema bourrellyi FEM_GT703

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Tychonema; Tychonema bourrellyi

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4322 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G4F3D2|A0A2G4F3D2_9CYAN Uncharacterized protein OS=Tychonema bourrellyi FEM_GT703 OX=2040638 GN=CP500_007815 PE=4 SV=1
MM1 pKa = 7.64SSTTRR6 pKa = 11.84EE7 pKa = 3.97QLHH10 pKa = 4.98QQIDD14 pKa = 4.07SLPDD18 pKa = 3.87HH19 pKa = 6.83IVEE22 pKa = 4.7QIASLILSITTQQKK36 pKa = 10.13IGISEE41 pKa = 4.12YY42 pKa = 10.73TDD44 pKa = 3.05WQSTQWQDD52 pKa = 3.17FSLMQLFCEE61 pKa = 4.61DD62 pKa = 4.66DD63 pKa = 4.1EE64 pKa = 5.48IEE66 pKa = 4.31YY67 pKa = 10.86SLEE70 pKa = 4.04DD71 pKa = 3.67AEE73 pKa = 5.93EE74 pKa = 4.65IYY76 pKa = 10.91QPP78 pKa = 4.11

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G4EVH5|A0A2G4EVH5_9CYAN Shikimate kinase OS=Tychonema bourrellyi FEM_GT703 OX=2040638 GN=aroK PE=3 SV=1
MM1 pKa = 6.86TQQTLHH7 pKa = 5.03GTSRR11 pKa = 11.84KK12 pKa = 9.46RR13 pKa = 11.84KK14 pKa = 7.05RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TATGRR29 pKa = 11.84LVIKK33 pKa = 10.39ARR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.48GRR40 pKa = 11.84HH41 pKa = 4.61RR42 pKa = 11.84LAVV45 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4322

0

4322

1342800

29

2561

310.7

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.36 ± 0.043

1.037 ± 0.015

5.133 ± 0.033

6.535 ± 0.043

3.904 ± 0.024

6.825 ± 0.046

1.666 ± 0.016

6.662 ± 0.029

5.015 ± 0.037

10.726 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.951 ± 0.017

4.276 ± 0.032

4.739 ± 0.031

5.006 ± 0.039

5.233 ± 0.026

6.496 ± 0.025

5.495 ± 0.038

6.647 ± 0.032

1.395 ± 0.017

2.899 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski