Streptococcus phage SW14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Brussowvirus; unclassified Brussowvirus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S5H1D7|A0A3S5H1D7_9CAUD Cro-like protein OS=Streptococcus phage SW14 OX=2419634 GN=SW14_029 PE=4 SV=1
MM1 pKa = 7.12VNWVDD6 pKa = 4.58KK7 pKa = 11.1DD8 pKa = 4.24GNDD11 pKa = 3.97LPDD14 pKa = 4.77GADD17 pKa = 3.18QDD19 pKa = 4.92FKK21 pKa = 11.67AGMYY25 pKa = 10.19FSFAGDD31 pKa = 3.52EE32 pKa = 4.3VNITDD37 pKa = 3.77TGEE40 pKa = 3.65GGYY43 pKa = 10.61YY44 pKa = 9.76GGYY47 pKa = 8.0YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S7W9Q1|A0A3S7W9Q1_9CAUD Terminase small subunit OS=Streptococcus phage SW14 OX=2419634 GN=SW14_002 PE=4 SV=1
MM1 pKa = 7.78NKK3 pKa = 9.6RR4 pKa = 11.84QRR6 pKa = 11.84KK7 pKa = 7.1KK8 pKa = 9.48QYY10 pKa = 9.02TRR12 pKa = 11.84AFSKK16 pKa = 10.79AYY18 pKa = 10.19DD19 pKa = 3.4EE20 pKa = 4.24SLKK23 pKa = 10.8YY24 pKa = 10.71KK25 pKa = 10.39HH26 pKa = 6.23FEE28 pKa = 4.02RR29 pKa = 11.84NISISTFTNPRR40 pKa = 11.84GIPIMFLSLNKK51 pKa = 10.17SINYY55 pKa = 8.7NFSHH59 pKa = 7.62DD60 pKa = 4.34EE61 pKa = 3.9LPEE64 pKa = 4.21ISIEE68 pKa = 4.2GCSVGYY74 pKa = 10.32KK75 pKa = 9.69PLKK78 pKa = 10.35DD79 pKa = 2.99WWW81 pKa = 3.97

Molecular weight: 9.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

10840

40

1545

235.7

26.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.694 ± 1.2

0.498 ± 0.122

6.061 ± 0.495

6.614 ± 0.76

4.013 ± 0.194

7.122 ± 0.519

1.301 ± 0.193

6.854 ± 0.332

7.712 ± 0.664

7.685 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.327

5.627 ± 0.429

3.044 ± 0.272

3.81 ± 0.279

4.308 ± 0.422

6.956 ± 0.657

6.411 ± 0.344

6.753 ± 0.269

1.255 ± 0.217

3.847 ± 0.458
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski