Apis mellifera associated microvirus 26
Average proteome isoelectric point is 7.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5T1|A0A3Q8U5T1_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 26 OX=2494754 PE=4 SV=1
MM1 pKa = 7.04 LQKK4 pKa = 10.71 LFAIRR9 pKa = 11.84 DD10 pKa = 3.68 SKK12 pKa = 10.17 TEE14 pKa = 3.8 YY15 pKa = 10.32 FGSPFCKK22 pKa = 9.37 ATHH25 pKa = 6.51 GEE27 pKa = 3.97 AEE29 pKa = 3.96 RR30 pKa = 11.84 DD31 pKa = 3.43 FRR33 pKa = 11.84 TLVNDD38 pKa = 3.66 EE39 pKa = 4.29 KK40 pKa = 10.78 TNLNQYY46 pKa = 9.92 PEE48 pKa = 5.63 DD49 pKa = 3.85 YY50 pKa = 10.79 DD51 pKa = 4.45 LYY53 pKa = 11.7 YY54 pKa = 10.91 LGEE57 pKa = 4.13 YY58 pKa = 10.31 DD59 pKa = 3.69 NNSGKK64 pKa = 10.39 FEE66 pKa = 4.18 TLEE69 pKa = 4.33 TPQHH73 pKa = 5.69 LMKK76 pKa = 10.72 AIQVKK81 pKa = 9.61 KK82 pKa = 10.26 QPEE85 pKa = 4.07 VV86 pKa = 3.16
Molecular weight: 10.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.995
IPC2_protein 5.143
IPC_protein 4.978
Toseland 4.902
ProMoST 5.105
Dawson 4.952
Bjellqvist 5.08
Wikipedia 4.813
Rodwell 4.863
Grimsley 4.825
Solomon 4.94
Lehninger 4.902
Nozaki 5.067
DTASelect 5.194
Thurlkill 4.902
EMBOSS 4.838
Sillero 5.143
Patrickios 3.859
IPC_peptide 4.952
IPC2_peptide 5.13
IPC2.peptide.svr19 5.185
Protein with the highest isoelectric point:
>tr|A0A3S8UU31|A0A3S8UU31_9VIRU Internal scaffolding protein OS=Apis mellifera associated microvirus 26 OX=2494754 PE=4 SV=1
MM1 pKa = 7.64 NLIQRR6 pKa = 11.84 NKK8 pKa = 10.58 NEE10 pKa = 4.08 TPLRR14 pKa = 11.84 TRR16 pKa = 11.84 SGVHH20 pKa = 5.3 PQNPFKK26 pKa = 10.22 RR27 pKa = 11.84 TKK29 pKa = 9.45 TALEE33 pKa = 4.03 PKK35 pKa = 8.55 PCVRR39 pKa = 11.84 ATRR42 pKa = 11.84 VRR44 pKa = 11.84 EE45 pKa = 3.71 KK46 pKa = 10.32 WLLGPFSFVKK56 pKa = 10.61 SPSLPPSPSPMCEE69 pKa = 4.1 KK70 pKa = 10.45 ILTSSPMCCTKK81 pKa = 10.21 QHH83 pKa = 5.52 NPRR86 pKa = 11.84 RR87 pKa = 11.84 NAAA90 pKa = 3.37
Molecular weight: 10.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.282
IPC2_protein 9.706
IPC_protein 10.452
Toseland 11.199
ProMoST 11.125
Dawson 11.228
Bjellqvist 10.979
Wikipedia 11.477
Rodwell 11.447
Grimsley 11.242
Solomon 11.477
Lehninger 11.433
Nozaki 11.184
DTASelect 10.979
Thurlkill 11.169
EMBOSS 11.623
Sillero 11.169
Patrickios 11.199
IPC_peptide 11.491
IPC2_peptide 10.306
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1378
83
525
196.9
22.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.2 ± 1.204
0.508 ± 0.346
4.717 ± 1.022
6.096 ± 0.818
4.209 ± 1.117
5.515 ± 0.536
2.758 ± 0.548
4.499 ± 0.395
7.837 ± 2.097
7.257 ± 0.526
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.54 ± 0.241
5.443 ± 0.728
6.894 ± 1.228
4.427 ± 0.353
5.515 ± 0.636
7.112 ± 1.02
6.241 ± 0.929
4.935 ± 0.716
1.814 ± 0.267
3.483 ± 0.855
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here