Capybara microvirus Cap3_SP_407
Average proteome isoelectric point is 5.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5T9|A0A4P8W5T9_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_407 OX=2585445 PE=4 SV=1
MM1 pKa = 7.71 SNFNGVNNLPRR12 pKa = 11.84 SLGDD16 pKa = 3.39 CYY18 pKa = 10.94 NYY20 pKa = 10.61 PFMSPSDD27 pKa = 3.39 ITDD30 pKa = 3.17 EE31 pKa = 4.16 NGITRR36 pKa = 11.84 VVFVCTPPPDD46 pKa = 4.82 LGTLDD51 pKa = 5.03 LYY53 pKa = 11.52 SDD55 pKa = 5.0 DD56 pKa = 4.2 VLANNGIYY64 pKa = 10.35 NVASFSPVGSRR75 pKa = 11.84 LSGISRR81 pKa = 11.84 VDD83 pKa = 3.05 KK84 pKa = 11.2 SLGDD88 pKa = 3.81 VVSKK92 pKa = 11.01 LPTPHH97 pKa = 7.06 PQPQPEE103 pKa = 4.34 TDD105 pKa = 3.11 NKK107 pKa = 10.97
Molecular weight: 11.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.301
IPC2_protein 4.368
IPC_protein 4.279
Toseland 4.05
ProMoST 4.457
Dawson 4.279
Bjellqvist 4.444
Wikipedia 4.266
Rodwell 4.101
Grimsley 3.961
Solomon 4.279
Lehninger 4.228
Nozaki 4.406
DTASelect 4.698
Thurlkill 4.126
EMBOSS 4.266
Sillero 4.406
Patrickios 3.465
IPC_peptide 4.266
IPC2_peptide 4.38
IPC2.peptide.svr19 4.333
Protein with the highest isoelectric point:
>tr|A0A4P8W613|A0A4P8W613_9VIRU Replication initiator protein OS=Capybara microvirus Cap3_SP_407 OX=2585445 PE=4 SV=1
MM1 pKa = 7.24 IAFMHH6 pKa = 6.37 FYY8 pKa = 10.5 VYY10 pKa = 10.52 KK11 pKa = 10.49 CYY13 pKa = 10.29 YY14 pKa = 10.01 LCANMYY20 pKa = 9.93 LCIVKK25 pKa = 10.13 SIKK28 pKa = 10.17 LIIMEE33 pKa = 4.33 IPNVRR38 pKa = 11.84 SIYY41 pKa = 10.33 GVFIKK46 pKa = 10.38 RR47 pKa = 11.84 VEE49 pKa = 4.1 SSLKK53 pKa = 10.52 RR54 pKa = 11.84 KK55 pKa = 9.36 GLQPLRR61 pKa = 11.84 FKK63 pKa = 10.94 LFKK66 pKa = 10.57 FDD68 pKa = 4.74 DD69 pKa = 3.77 SSVYY73 pKa = 10.31 QLVILPSNDD82 pKa = 3.14 FGCFCQNTIVVVSRR96 pKa = 11.84 LCDD99 pKa = 3.12 KK100 pKa = 10.92 FGYY103 pKa = 10.19 NFLIGVEE110 pKa = 4.23 KK111 pKa = 10.91 NGTEE115 pKa = 3.78 FVPYY119 pKa = 9.94 FFVNFF124 pKa = 3.83
Molecular weight: 14.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.631
IPC2_protein 8.419
IPC_protein 8.346
Toseland 8.507
ProMoST 8.756
Dawson 9.033
Bjellqvist 9.165
Wikipedia 9.107
Rodwell 9.107
Grimsley 8.521
Solomon 9.18
Lehninger 9.151
Nozaki 9.297
DTASelect 8.975
Thurlkill 9.019
EMBOSS 9.194
Sillero 9.282
Patrickios 3.3
IPC_peptide 9.165
IPC2_peptide 8.2
IPC2.peptide.svr19 8.001
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1591
107
581
318.2
36.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.783 ± 1.286
1.32 ± 0.436
6.223 ± 0.824
4.965 ± 1.0
5.468 ± 1.018
5.531 ± 0.555
1.697 ± 0.582
5.343 ± 0.427
5.343 ± 0.486
8.171 ± 0.547
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.074 ± 0.218
5.72 ± 0.731
4.714 ± 0.813
4.337 ± 0.994
4.84 ± 0.599
9.302 ± 0.837
5.657 ± 0.913
5.72 ± 0.924
1.006 ± 0.256
6.788 ± 0.995
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here