Tortoise microvirus 33

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W671|A0A4P8W671_9VIRU Major capsid protein OS=Tortoise microvirus 33 OX=2583136 PE=3 SV=1
MM1 pKa = 7.99LFRR4 pKa = 11.84TQYY7 pKa = 11.36NFVLGNSEE15 pKa = 4.19TDD17 pKa = 3.1VTTITEE23 pKa = 4.42PSQTVPDD30 pKa = 3.73EE31 pKa = 4.19SLTIRR36 pKa = 11.84EE37 pKa = 3.83IFEE40 pKa = 4.23RR41 pKa = 11.84YY42 pKa = 7.62ATGRR46 pKa = 11.84PLDD49 pKa = 3.81VVDD52 pKa = 3.74SRR54 pKa = 11.84GIYY57 pKa = 9.83TEE59 pKa = 4.63DD60 pKa = 4.26KK61 pKa = 10.74VGKK64 pKa = 10.32DD65 pKa = 3.93PDD67 pKa = 3.86FDD69 pKa = 6.61DD70 pKa = 6.08YY71 pKa = 12.17DD72 pKa = 4.33DD73 pKa = 5.52LCDD76 pKa = 4.14PNADD80 pKa = 3.0ISDD83 pKa = 3.83FHH85 pKa = 7.78AQAAYY90 pKa = 9.82EE91 pKa = 3.98YY92 pKa = 10.63AYY94 pKa = 10.52MLDD97 pKa = 3.35EE98 pKa = 4.81EE99 pKa = 4.55KK100 pKa = 10.56RR101 pKa = 11.84KK102 pKa = 10.01KK103 pKa = 10.59DD104 pKa = 3.25SAKK107 pKa = 10.36KK108 pKa = 9.84KK109 pKa = 9.59RR110 pKa = 11.84QEE112 pKa = 4.07EE113 pKa = 3.44FDD115 pKa = 3.61KK116 pKa = 10.93RR117 pKa = 11.84VKK119 pKa = 10.75EE120 pKa = 3.97EE121 pKa = 3.92AQRR124 pKa = 11.84MMNDD128 pKa = 3.25NSQSSDD134 pKa = 3.47DD135 pKa = 4.31AKK137 pKa = 11.33VEE139 pKa = 4.25SS140 pKa = 4.18

Molecular weight: 16.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FVX8|A0A4V1FVX8_9VIRU DNA pilot protein OS=Tortoise microvirus 33 OX=2583136 PE=4 SV=1
MM1 pKa = 7.62SILGSAAIAGGASLIGGAISGLFGSKK27 pKa = 9.47SSKK30 pKa = 10.04KK31 pKa = 10.06ANQSNEE37 pKa = 3.57RR38 pKa = 11.84LAAQQNEE45 pKa = 4.41YY46 pKa = 10.46NLQYY50 pKa = 10.27QANEE54 pKa = 3.68FAQNQKK60 pKa = 9.71MSDD63 pKa = 3.18LEE65 pKa = 4.11YY66 pKa = 10.85QRR68 pKa = 11.84NLEE71 pKa = 3.96QWHH74 pKa = 6.19LQNEE78 pKa = 4.66YY79 pKa = 10.94NSPAQQRR86 pKa = 11.84QRR88 pKa = 11.84LVEE91 pKa = 4.64AGLNPNLVYY100 pKa = 10.9GSGTASAGNATGSPQYY116 pKa = 10.31QAARR120 pKa = 11.84YY121 pKa = 7.24KK122 pKa = 10.9APNAEE127 pKa = 4.2RR128 pKa = 11.84ATVHH132 pKa = 5.82PQGFVPPAIDD142 pKa = 4.16PYY144 pKa = 10.57QAVGLGQQLAMQKK157 pKa = 10.34AQVSQLAAQTEE168 pKa = 4.64FTKK171 pKa = 10.83QQTANAALEE180 pKa = 4.44SLFKK184 pKa = 9.73TARR187 pKa = 11.84TAGVHH192 pKa = 6.54LDD194 pKa = 3.57NAKK197 pKa = 10.35KK198 pKa = 10.61SSLFDD203 pKa = 3.84LSIDD207 pKa = 3.51QARR210 pKa = 11.84ANLAKK215 pKa = 9.04TQSSTSLDD223 pKa = 3.54SARR226 pKa = 11.84LDD228 pKa = 3.56LQRR231 pKa = 11.84VLNDD235 pKa = 3.4RR236 pKa = 11.84ARR238 pKa = 11.84ALAPLQVKK246 pKa = 8.93QLQTSIDD253 pKa = 3.67RR254 pKa = 11.84LKK256 pKa = 9.94VTRR259 pKa = 11.84DD260 pKa = 2.85LDD262 pKa = 3.72AFRR265 pKa = 11.84LQLLRR270 pKa = 11.84LGISDD275 pKa = 3.82KK276 pKa = 11.38DD277 pKa = 3.74GLVSRR282 pKa = 11.84LAARR286 pKa = 11.84WLIDD290 pKa = 3.49PANSSAVDD298 pKa = 3.76SIKK301 pKa = 11.12NFFSPSQFVKK311 pKa = 10.55DD312 pKa = 3.55VRR314 pKa = 11.84HH315 pKa = 6.26DD316 pKa = 3.91VGAIWNIFKK325 pKa = 9.06KK326 pKa = 8.88TPRR329 pKa = 11.84GFIGRR334 pKa = 3.9

Molecular weight: 36.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1294

140

539

323.5

36.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.578 ± 1.552

0.773 ± 0.498

6.801 ± 1.12

4.946 ± 0.847

5.1 ± 0.541

6.105 ± 0.828

1.855 ± 0.311

5.023 ± 0.784

5.41 ± 1.335

8.501 ± 0.873

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.7 ± 0.268

4.173 ± 0.57

4.946 ± 0.739

5.255 ± 1.621

7.032 ± 0.623

8.346 ± 0.28

4.791 ± 0.373

5.023 ± 0.446

1.082 ± 0.191

4.56 ± 0.796
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski