Sparganum proliferum
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22389 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A654I8C3|A0A654I8C3_9CEST Reverse transcriptase domain-containing protein OS=Sparganum proliferum OX=64606 GN=SPRV2_LOCUS22653 PE=4 SV=1
PP1 pKa = 7.02 SAHH4 pKa = 6.76 YY5 pKa = 10.79 DD6 pKa = 4.46 DD7 pKa = 5.71 IDD9 pKa = 4.13 VDD11 pKa = 4.39 DD12 pKa = 5.48 DD13 pKa = 3.85 VAAAAVDD20 pKa = 4.19 DD21 pKa = 5.3 ADD23 pKa = 5.91 GDD25 pKa = 5.06 DD26 pKa = 6.18 DD27 pKa = 7.05 DD28 pKa = 7.73 DD29 pKa = 7.72 DD30 pKa = 7.61 DD31 pKa = 7.68 DD32 pKa = 7.65 DD33 pKa = 7.65 DD34 pKa = 7.65 DD35 pKa = 7.65 DD36 pKa = 7.65 DD37 pKa = 7.65 DD38 pKa = 7.65 DD39 pKa = 7.61 DD40 pKa = 7.44 DD41 pKa = 7.09 DD42 pKa = 6.58 DD43 pKa = 6.51 GGGGGCSVSGPSHH56 pKa = 6.99 HH57 pKa = 7.99 DD58 pKa = 2.89 IRR60 pKa = 11.84 PQTRR64 pKa = 11.84 DD65 pKa = 2.68 KK66 pKa = 10.78 RR67 pKa = 11.84 TIKK70 pKa = 10.74 VMTPVHH76 pKa = 5.81 TFFVLIGHH84 pKa = 7.1 PSAYY88 pKa = 10.08 DD89 pKa = 3.37 SPPCHH94 pKa = 6.94 ISDD97 pKa = 4.44 LVYY100 pKa = 10.58 KK101 pKa = 10.05 PPHH104 pKa = 6.28 LCCLSYY110 pKa = 10.65 SVHH113 pKa = 6.75 VLFPINLLFCIHH125 pKa = 6.81 NIGNATT131 pKa = 3.41
Molecular weight: 14.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.688
IPC2_protein 3.757
IPC_protein 3.821
Toseland 3.567
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.859
Rodwell 3.63
Grimsley 3.465
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.342
Thurlkill 3.643
EMBOSS 3.859
Sillero 3.948
Patrickios 0.896
IPC_peptide 3.834
IPC2_peptide 3.923
IPC2.peptide.svr19 3.825
Protein with the highest isoelectric point:
>tr|A0A654GFY6|A0A654GFY6_9CEST Uncharacterized protein OS=Sparganum proliferum OX=64606 GN=SPRV2_LOCUS20 PE=4 SV=1
RR1 pKa = 7.24 RR2 pKa = 11.84 RR3 pKa = 11.84 WRR5 pKa = 11.84 GWWRR9 pKa = 11.84 NRR11 pKa = 11.84 RR12 pKa = 11.84 WRR14 pKa = 11.84 RR15 pKa = 11.84 SRR17 pKa = 11.84 RR18 pKa = 11.84 WRR20 pKa = 11.84 RR21 pKa = 11.84 NRR23 pKa = 11.84 RR24 pKa = 11.84 WRR26 pKa = 11.84 SRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 IGRR33 pKa = 11.84 WARR36 pKa = 11.84 WWTRR40 pKa = 11.84 RR41 pKa = 11.84 WTRR44 pKa = 11.84 RR45 pKa = 11.84 WTRR48 pKa = 11.84 RR49 pKa = 11.84 WTCC52 pKa = 2.99
Molecular weight: 7.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22389
0
22389
9039214
13
10665
403.7
44.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.021 ± 0.016
2.05 ± 0.01
5.402 ± 0.015
5.697 ± 0.02
3.782 ± 0.014
5.656 ± 0.018
2.7 ± 0.007
4.36 ± 0.013
4.31 ± 0.015
9.805 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.932 ± 0.008
3.683 ± 0.009
6.388 ± 0.022
4.184 ± 0.012
7.001 ± 0.017
8.979 ± 0.027
6.533 ± 0.017
6.131 ± 0.014
1.07 ± 0.005
2.307 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here