Chicken anemia virus (isolate Japan 82-2) (CAV)
Average proteome isoelectric point is 8.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|P54096|VP3_CAV82 Apoptin OS=Chicken anemia virus (isolate Japan 82-2) OX=73476 GN=VP3 PE=2 SV=1
MM1 pKa = 7.54 HH2 pKa = 7.49 GNGGQPAAGGSEE14 pKa = 4.12 SALSRR19 pKa = 11.84 EE20 pKa = 4.43 GQPGPSGAAQGQVISNEE37 pKa = 3.83 RR38 pKa = 11.84 SPRR41 pKa = 11.84 RR42 pKa = 11.84 YY43 pKa = 8.28 STRR46 pKa = 11.84 TINGVQATNKK56 pKa = 7.8 FTAVGNPSLQRR67 pKa = 11.84 DD68 pKa = 3.84 PDD70 pKa = 3.55 WYY72 pKa = 9.71 RR73 pKa = 11.84 WNYY76 pKa = 8.1 NHH78 pKa = 7.22 SIAVWLRR85 pKa = 11.84 EE86 pKa = 4.05 CSRR89 pKa = 11.84 SHH91 pKa = 7.08 AKK93 pKa = 9.51 ICNCGQFRR101 pKa = 11.84 KK102 pKa = 9.59 HH103 pKa = 5.49 WFQEE107 pKa = 4.39 CAGLEE112 pKa = 4.08 DD113 pKa = 5.22 RR114 pKa = 11.84 STQASLEE121 pKa = 4.13 EE122 pKa = 5.15 AILRR126 pKa = 11.84 PLRR129 pKa = 11.84 VQGKK133 pKa = 7.45 RR134 pKa = 11.84 AKK136 pKa = 10.3 RR137 pKa = 11.84 KK138 pKa = 9.49 LDD140 pKa = 3.32 YY141 pKa = 10.37 HH142 pKa = 6.72 YY143 pKa = 11.12 SQPTPNRR150 pKa = 11.84 KK151 pKa = 8.91 KK152 pKa = 9.96 VYY154 pKa = 9.0 KK155 pKa = 7.97 TVRR158 pKa = 11.84 WKK160 pKa = 11.07 DD161 pKa = 3.51 EE162 pKa = 4.08 LADD165 pKa = 4.19 RR166 pKa = 11.84 EE167 pKa = 4.28 ADD169 pKa = 3.67 FTPSEE174 pKa = 4.2 EE175 pKa = 4.57 DD176 pKa = 3.16 GGTTSSDD183 pKa = 3.08 FDD185 pKa = 3.81 EE186 pKa = 6.39 DD187 pKa = 3.83 INFDD191 pKa = 3.22 IGGDD195 pKa = 3.55 SGIVDD200 pKa = 4.09 EE201 pKa = 4.93 LLGRR205 pKa = 11.84 PFTTPAPVRR214 pKa = 11.84 IVV216 pKa = 2.92
Molecular weight: 24.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.09
IPC2_protein 7.395
IPC_protein 7.468
Toseland 7.146
ProMoST 7.892
Dawson 8.083
Bjellqvist 8.346
Wikipedia 8.009
Rodwell 8.068
Grimsley 7.176
Solomon 8.229
Lehninger 8.258
Nozaki 8.58
DTASelect 8.17
Thurlkill 8.214
EMBOSS 8.317
Sillero 8.536
Patrickios 4.622
IPC_peptide 8.229
IPC2_peptide 7.541
IPC2.peptide.svr19 7.552
Protein with the highest isoelectric point:
>sp|P54093|VP2_CAV82 Dual specificity protein phosphatase VP2 OS=Chicken anemia virus (isolate Japan 82-2) OX=73476 GN=VP2 PE=2 SV=1
MM1 pKa = 7.42 EE2 pKa = 4.52 RR3 pKa = 11.84 RR4 pKa = 11.84 ARR6 pKa = 11.84 RR7 pKa = 11.84 PRR9 pKa = 11.84 GRR11 pKa = 11.84 FYY13 pKa = 11.27 AFRR16 pKa = 11.84 RR17 pKa = 11.84 GRR19 pKa = 11.84 WNHH22 pKa = 5.65 LKK24 pKa = 10.47 RR25 pKa = 11.84 LRR27 pKa = 11.84 RR28 pKa = 11.84 RR29 pKa = 11.84 YY30 pKa = 9.6 KK31 pKa = 8.5 FRR33 pKa = 11.84 HH34 pKa = 5.64 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 QRR39 pKa = 11.84 YY40 pKa = 7.89 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 AFRR46 pKa = 11.84 KK47 pKa = 9.08 AFHH50 pKa = 6.5 NPRR53 pKa = 11.84 PGTYY57 pKa = 9.13 SVRR60 pKa = 11.84 LPNPQSTMTIRR71 pKa = 11.84 FQGVIFLTEE80 pKa = 3.98 GLILPKK86 pKa = 10.65 NSTAGGYY93 pKa = 9.63 ADD95 pKa = 3.85 HH96 pKa = 7.07 MYY98 pKa = 10.18 GARR101 pKa = 11.84 VAKK104 pKa = 10.39 ISVNLKK110 pKa = 9.73 EE111 pKa = 4.38 FLLASMNLTYY121 pKa = 10.55 VSKK124 pKa = 10.97 LGGPIAGEE132 pKa = 4.41 LIADD136 pKa = 4.38 GSKK139 pKa = 10.61 SEE141 pKa = 5.18 AAEE144 pKa = 3.93 NWPNCCVPLDD154 pKa = 4.04 NNVPSATPSAWWRR167 pKa = 11.84 WALMMMQPTDD177 pKa = 2.89 SCRR180 pKa = 11.84 FFNHH184 pKa = 6.69 PKK186 pKa = 10.56 QMTLQDD192 pKa = 3.9 MGRR195 pKa = 11.84 MFGGWHH201 pKa = 7.18 LFRR204 pKa = 11.84 HH205 pKa = 5.88 IEE207 pKa = 4.02 TRR209 pKa = 11.84 FQLLATKK216 pKa = 10.66 NEE218 pKa = 4.19 GSFSPVASLLSQGEE232 pKa = 4.35 YY233 pKa = 8.83 LTRR236 pKa = 11.84 RR237 pKa = 11.84 DD238 pKa = 3.7 DD239 pKa = 3.65 VKK241 pKa = 11.26 YY242 pKa = 11.07 SSDD245 pKa = 3.15 HH246 pKa = 4.48 QNRR249 pKa = 11.84 WRR251 pKa = 11.84 KK252 pKa = 9.35 GGQPMTGGIAYY263 pKa = 7.3 ATGKK267 pKa = 8.59 MRR269 pKa = 11.84 PDD271 pKa = 3.42 EE272 pKa = 4.15 QQYY275 pKa = 9.59 PAMPPDD281 pKa = 3.92 PPIITSTTAQGTQVRR296 pKa = 11.84 CMNSTQAWWSWDD308 pKa = 3.04 TYY310 pKa = 11.0 MSFATLTALGAQWSFPPGQRR330 pKa = 11.84 SVSRR334 pKa = 11.84 RR335 pKa = 11.84 SFNHH339 pKa = 5.11 HH340 pKa = 6.41 KK341 pKa = 10.76 ARR343 pKa = 11.84 GAGDD347 pKa = 3.45 PKK349 pKa = 9.58 GQRR352 pKa = 11.84 WHH354 pKa = 6.22 TLVPLGTEE362 pKa = 4.78 TITDD366 pKa = 3.72 SYY368 pKa = 10.29 MGAPASEE375 pKa = 4.7 LDD377 pKa = 3.69 TNFFTLYY384 pKa = 9.98 VAQGTNKK391 pKa = 9.19 SQQYY395 pKa = 10.29 KK396 pKa = 10.49 FGTATYY402 pKa = 9.86 ALKK405 pKa = 10.54 EE406 pKa = 4.03 PVMKK410 pKa = 10.25 SDD412 pKa = 2.48 SWAVVRR418 pKa = 11.84 VQSVWQLGNRR428 pKa = 11.84 QRR430 pKa = 11.84 PYY432 pKa = 10.08 PWDD435 pKa = 3.8 VNWANSTMYY444 pKa = 10.02 WGTQPP449 pKa = 4.53
Molecular weight: 51.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.882
IPC_protein 10.76
Toseland 10.643
ProMoST 10.423
Dawson 10.789
Bjellqvist 10.54
Wikipedia 11.023
Rodwell 10.95
Grimsley 10.847
Solomon 10.891
Lehninger 10.847
Nozaki 10.628
DTASelect 10.526
Thurlkill 10.672
EMBOSS 11.052
Sillero 10.716
Patrickios 10.599
IPC_peptide 10.891
IPC2_peptide 9.56
IPC2.peptide.svr19 8.374
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
786
121
449
262.0
29.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.506 ± 0.266
1.654 ± 0.722
4.453 ± 0.943
4.326 ± 1.182
3.69 ± 0.653
7.506 ± 1.108
2.163 ± 0.365
3.308 ± 0.682
4.326 ± 0.142
6.234 ± 0.568
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.545 ± 1.19
4.326 ± 0.29
7.379 ± 1.409
4.835 ± 0.873
10.051 ± 0.5
8.015 ± 0.813
7.634 ± 1.468
4.071 ± 0.464
2.799 ± 0.867
3.181 ± 0.728
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here