Chelonia mydas (Green sea-turtle) (Chelonia agassizi)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18960 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M7BXW5|M7BXW5_CHEMY EH domain-binding protein 1 OS=Chelonia mydas OX=8469 GN=UY3_09947 PE=4 SV=1
MM1 pKa = 7.32WAAVWPWTRR10 pKa = 11.84ALLLLLLFPRR20 pKa = 11.84PLAQGFLLLPGGSPAALLGLGCVLLLGLFGAALPGTLKK58 pKa = 11.05GFDD61 pKa = 3.21QTINLILDD69 pKa = 3.75EE70 pKa = 4.1SHH72 pKa = 6.5EE73 pKa = 4.5RR74 pKa = 11.84VFSSSQGVEE83 pKa = 3.89QVVLGLYY90 pKa = 9.48IVRR93 pKa = 11.84GDD95 pKa = 3.74NVAAAALDD103 pKa = 4.18NIFGTGGVIGSDD115 pKa = 2.73WCYY118 pKa = 10.72GASCTFGTDD127 pKa = 3.2DD128 pKa = 3.67GTGTSIYY135 pKa = 10.0TNIVIDD141 pKa = 4.31AGCDD145 pKa = 3.61GVDD148 pKa = 3.81EE149 pKa = 5.55DD150 pKa = 5.12PGADD154 pKa = 3.25ATPGVV159 pKa = 3.96

Molecular weight: 16.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M7ANA5|M7ANA5_CHEMY 2-phospho-D-glycerate hydro-lyase OS=Chelonia mydas OX=8469 GN=UY3_17006 PE=3 SV=1
MM1 pKa = 7.34LASTAGALRR10 pKa = 11.84PLPVMLASTAGALRR24 pKa = 11.84PLPVMLASTAGALRR38 pKa = 11.84PLPVMLASTAGALRR52 pKa = 11.84PLPVMLASTAGALRR66 pKa = 11.84PLPVMLASTAGALRR80 pKa = 11.84PLPVMLASTAGALRR94 pKa = 11.84PLPVMLASTAGALRR108 pKa = 11.84PLPVMLASTAGALRR122 pKa = 11.84PLPVMLASTAGALRR136 pKa = 11.84PLPVMLASTAGALRR150 pKa = 11.84PLPVMLASTAGALRR164 pKa = 11.84PLPVMLASTAGALRR178 pKa = 11.84PLPVMLASTAGALRR192 pKa = 11.84PLPVMLASTAGALRR206 pKa = 11.84PLPVMLASTAGALRR220 pKa = 11.84PLPVMLASTAGALRR234 pKa = 11.84PLPVMLASTAGALRR248 pKa = 11.84PLPVMLASTAGALRR262 pKa = 11.84PLPVMLASTARR273 pKa = 11.84ALRR276 pKa = 11.84PLPVMLAVVRR286 pKa = 4.34

Molecular weight: 28.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18960

0

18960

9158208

35

34967

483.0

54.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.688 ± 0.016

2.312 ± 0.015

4.901 ± 0.012

7.198 ± 0.025

3.637 ± 0.012

6.1 ± 0.021

2.599 ± 0.011

4.72 ± 0.015

6.074 ± 0.022

9.678 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.009

3.914 ± 0.013

5.637 ± 0.021

4.832 ± 0.017

5.387 ± 0.014

8.431 ± 0.024

5.509 ± 0.016

6.071 ± 0.017

1.192 ± 0.007

2.802 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski