Sida yellow net virus
Average proteome isoelectric point is 7.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L7YFH4|L7YFH4_9GEMI Capsid protein OS=Sida yellow net virus OX=1266977 PE=3 SV=1
MM1 pKa = 7.28 PRR3 pKa = 11.84 KK4 pKa = 10.11 GSFCIKK10 pKa = 9.77 AKK12 pKa = 10.62 NYY14 pKa = 9.39 FITYY18 pKa = 7.6 PRR20 pKa = 11.84 CSLTKK25 pKa = 10.62 EE26 pKa = 3.94 EE27 pKa = 4.55 TLSQFLDD34 pKa = 3.05 IQTPVNKK41 pKa = 10.0 KK42 pKa = 9.66 FIKK45 pKa = 9.67 ICRR48 pKa = 11.84 EE49 pKa = 3.52 LHH51 pKa = 6.26 EE52 pKa = 4.93 NGEE55 pKa = 4.25 PHH57 pKa = 6.94 LHH59 pKa = 6.18 VLLQFEE65 pKa = 5.61 GKK67 pKa = 9.17 FQCTNNRR74 pKa = 11.84 LFDD77 pKa = 4.14 LVSPTRR83 pKa = 11.84 SAHH86 pKa = 4.86 FHH88 pKa = 6.55 PNIQGAKK95 pKa = 9.25 SSSDD99 pKa = 3.11 VKK101 pKa = 11.24 SYY103 pKa = 10.79 IDD105 pKa = 3.8 KK106 pKa = 11.28 DD107 pKa = 3.26 GDD109 pKa = 3.91 TIEE112 pKa = 4.14 WGQFQVDD119 pKa = 3.41 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTANDD132 pKa = 3.91 AAAEE136 pKa = 4.01 ALNAPDD142 pKa = 4.52 KK143 pKa = 10.32 QTALQIIRR151 pKa = 11.84 EE152 pKa = 3.99 KK153 pKa = 10.97 LPEE156 pKa = 4.14 KK157 pKa = 10.59 FLFQFHH163 pKa = 6.65 NLNSNLNRR171 pKa = 11.84 IFTKK175 pKa = 10.51 APEE178 pKa = 4.04 PWVPPFPLSSFTNVPDD194 pKa = 5.1 EE195 pKa = 4.13 MQEE198 pKa = 3.8 WADD201 pKa = 3.73 EE202 pKa = 4.25 YY203 pKa = 11.01 FGRR206 pKa = 11.84 GSAARR211 pKa = 11.84 PLRR214 pKa = 11.84 PMSLIVEE221 pKa = 4.74 GDD223 pKa = 3.31 SRR225 pKa = 11.84 TGKK228 pKa = 8.55 TMWARR233 pKa = 11.84 ALGPHH238 pKa = 6.63 NYY240 pKa = 10.14 LSGHH244 pKa = 6.9 LDD246 pKa = 3.48 FNSRR250 pKa = 11.84 VYY252 pKa = 11.18 SNEE255 pKa = 3.29 VEE257 pKa = 4.32 YY258 pKa = 11.37 NVIDD262 pKa = 4.44 DD263 pKa = 4.88 VNPQYY268 pKa = 11.47 LKK270 pKa = 10.5 LKK272 pKa = 9.13 HH273 pKa = 5.51 WKK275 pKa = 9.67 EE276 pKa = 3.98 LIGAQKK282 pKa = 10.57 DD283 pKa = 3.42 WQSNCKK289 pKa = 9.43 YY290 pKa = 10.35 GKK292 pKa = 10.2 ASSKK296 pKa = 10.85 LKK298 pKa = 10.51 VGYY301 pKa = 8.93 HH302 pKa = 4.94 QSCSAIQGEE311 pKa = 4.5 GVSYY315 pKa = 10.94 KK316 pKa = 10.58 EE317 pKa = 4.03 YY318 pKa = 10.79 LDD320 pKa = 4.04 KK321 pKa = 11.53 EE322 pKa = 4.13 EE323 pKa = 4.03 NAALKK328 pKa = 10.48 SWTLHH333 pKa = 4.44 NAKK336 pKa = 10.29 FIFLDD341 pKa = 3.55 SPLYY345 pKa = 10.59 QSATQSGEE353 pKa = 3.93 EE354 pKa = 4.32 EE355 pKa = 4.28 RR356 pKa = 11.84 NSPP359 pKa = 4.22
Molecular weight: 40.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.72
IPC2_protein 6.912
IPC_protein 7.0
Toseland 6.927
ProMoST 7.571
Dawson 7.6
Bjellqvist 7.644
Wikipedia 7.541
Rodwell 7.6
Grimsley 7.029
Solomon 7.673
Lehninger 7.688
Nozaki 7.936
DTASelect 7.775
Thurlkill 7.819
EMBOSS 7.878
Sillero 8.039
Patrickios 4.355
IPC_peptide 7.673
IPC2_peptide 7.161
IPC2.peptide.svr19 7.182
Protein with the highest isoelectric point:
>tr|L7YFH4|L7YFH4_9GEMI Capsid protein OS=Sida yellow net virus OX=1266977 PE=3 SV=1
MM1 pKa = 7.94 PKK3 pKa = 9.54 RR4 pKa = 11.84 DD5 pKa = 3.67 PSWRR9 pKa = 11.84 QMAGTSKK16 pKa = 10.62 VSRR19 pKa = 11.84 TSNFSPRR26 pKa = 11.84 GGGGPKK32 pKa = 9.71 FNKK35 pKa = 9.66 ASEE38 pKa = 4.21 WVNRR42 pKa = 11.84 PMYY45 pKa = 9.79 RR46 pKa = 11.84 KK47 pKa = 8.95 PRR49 pKa = 11.84 IYY51 pKa = 9.6 RR52 pKa = 11.84 TLRR55 pKa = 11.84 TPDD58 pKa = 3.21 VPRR61 pKa = 11.84 GCEE64 pKa = 4.34 GPCKK68 pKa = 9.32 VQSYY72 pKa = 6.94 EE73 pKa = 3.74 QRR75 pKa = 11.84 HH76 pKa = 6.38 DD77 pKa = 3.16 ISHH80 pKa = 6.13 VGKK83 pKa = 10.58 VMCISDD89 pKa = 3.41 ITRR92 pKa = 11.84 GNGITHH98 pKa = 6.78 RR99 pKa = 11.84 VGKK102 pKa = 9.44 RR103 pKa = 11.84 FCVKK107 pKa = 9.92 SVYY110 pKa = 10.24 ILGKK114 pKa = 9.73 VWMDD118 pKa = 3.29 EE119 pKa = 4.01 NIKK122 pKa = 10.61 LKK124 pKa = 10.79 NHH126 pKa = 6.24 TNSCMFWLVRR136 pKa = 11.84 DD137 pKa = 3.74 RR138 pKa = 11.84 RR139 pKa = 11.84 PYY141 pKa = 8.05 GTPMDD146 pKa = 4.6 FGQVFNMFDD155 pKa = 3.99 NEE157 pKa = 4.11 PSTATVKK164 pKa = 10.85 NDD166 pKa = 2.82 LRR168 pKa = 11.84 DD169 pKa = 3.54 RR170 pKa = 11.84 FQVMHH175 pKa = 6.97 KK176 pKa = 9.73 FYY178 pKa = 11.33 AKK180 pKa = 9.12 VTGGQYY186 pKa = 11.1 ASNEE190 pKa = 3.73 QALVKK195 pKa = 10.5 RR196 pKa = 11.84 FWKK199 pKa = 10.3 VNNHH203 pKa = 4.48 VVYY206 pKa = 10.67 NHH208 pKa = 5.8 QEE210 pKa = 3.47 AGKK213 pKa = 10.41 YY214 pKa = 8.39 EE215 pKa = 4.09 NHH217 pKa = 6.52 TEE219 pKa = 3.99 NALLLYY225 pKa = 7.29 MACTHH230 pKa = 7.07 ASNPVYY236 pKa = 9.86 ATLKK240 pKa = 9.47 IRR242 pKa = 11.84 IYY244 pKa = 10.59 FYY246 pKa = 11.38 DD247 pKa = 4.2 SITNN251 pKa = 3.6
Molecular weight: 29.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.229
IPC2_protein 9.355
IPC_protein 9.385
Toseland 9.911
ProMoST 9.663
Dawson 10.16
Bjellqvist 9.867
Wikipedia 10.335
Rodwell 10.496
Grimsley 10.233
Solomon 10.175
Lehninger 10.131
Nozaki 9.97
DTASelect 9.838
Thurlkill 9.999
EMBOSS 10.335
Sillero 10.072
Patrickios 9.604
IPC_peptide 10.175
IPC2_peptide 8.712
IPC2.peptide.svr19 8.219
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
956
85
359
191.2
21.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.021 ± 0.872
1.883 ± 0.476
4.916 ± 0.737
5.021 ± 1.013
4.393 ± 0.507
5.753 ± 0.516
3.661 ± 0.595
5.23 ± 0.606
5.962 ± 1.191
6.904 ± 1.19
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.301 ± 0.696
5.962 ± 0.497
5.126 ± 0.708
4.812 ± 0.754
7.113 ± 1.255
8.264 ± 1.545
6.067 ± 1.016
5.649 ± 1.085
1.778 ± 0.126
4.184 ± 0.879
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here