Hubei unio douglasiae virus 1
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KGU5|A0A1L3KGU5_9VIRU Capsid protein beta OS=Hubei unio douglasiae virus 1 OX=1923321 PE=4 SV=1
MM1 pKa = 7.15 TLAGEE6 pKa = 4.7 GFLKK10 pKa = 10.81 CAFAPPDD17 pKa = 3.95 FNSDD21 pKa = 3.11 AGRR24 pKa = 11.84 GIPDD28 pKa = 3.95 DD29 pKa = 3.85 FQGKK33 pKa = 8.95 VLTRR37 pKa = 11.84 KK38 pKa = 9.97 DD39 pKa = 3.6 VLTTSLSYY47 pKa = 11.02 GAGQDD52 pKa = 3.47 VYY54 pKa = 11.01 ILVAPTPGISYY65 pKa = 8.84 WYY67 pKa = 10.32 ASVANGASLSGTAFRR82 pKa = 11.84 PNPYY86 pKa = 9.24 PGYY89 pKa = 10.07 RR90 pKa = 11.84 SLFGDD95 pKa = 3.59 TGLEE99 pKa = 3.81 RR100 pKa = 11.84 ANNVTSFRR108 pKa = 11.84 YY109 pKa = 10.03 ASTCVGLYY117 pKa = 6.81 PTSNMQQFAGSISVWKK133 pKa = 10.35 FPMEE137 pKa = 5.3 LINQIVGVGTPVVGQPCWVLNGTDD161 pKa = 3.3 NFSGIGYY168 pKa = 9.76 EE169 pKa = 4.02 NYY171 pKa = 9.3 TDD173 pKa = 4.07 SFIKK177 pKa = 10.54 GMYY180 pKa = 8.48 SQSVCNEE187 pKa = 3.51 PDD189 pKa = 3.15 FEE191 pKa = 4.13 FRR193 pKa = 11.84 PIIEE197 pKa = 4.88 EE198 pKa = 3.59 IGAIPSGVITATQAGMFCTFATSQPAAGQLCGIVGMGTMDD238 pKa = 5.36 CILIKK243 pKa = 10.7 VATPVGATNSAVLKK257 pKa = 8.96 TWACVEE263 pKa = 3.93 YY264 pKa = 10.66 RR265 pKa = 11.84 PNTNSALYY273 pKa = 10.06 SYY275 pKa = 10.91 AHH277 pKa = 7.35 DD278 pKa = 4.3 SPAPDD283 pKa = 4.07 LMALAAYY290 pKa = 10.19 RR291 pKa = 11.84 EE292 pKa = 4.13 IAKK295 pKa = 9.59 NVPIAVPCSQNEE307 pKa = 4.43 GFWKK311 pKa = 10.25 RR312 pKa = 11.84 VLTILRR318 pKa = 11.84 GGLSLTSKK326 pKa = 10.48 IPGPIGMTATGVSGIMDD343 pKa = 4.46 MMTGLATT350 pKa = 3.64
Molecular weight: 37.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.946
IPC2_protein 5.143
IPC_protein 5.003
Toseland 4.851
ProMoST 5.118
Dawson 4.965
Bjellqvist 5.105
Wikipedia 4.851
Rodwell 4.851
Grimsley 4.762
Solomon 4.965
Lehninger 4.914
Nozaki 5.08
DTASelect 5.258
Thurlkill 4.863
EMBOSS 4.863
Sillero 5.13
Patrickios 1.062
IPC_peptide 4.965
IPC2_peptide 5.118
IPC2.peptide.svr19 5.07
Protein with the highest isoelectric point:
>tr|A0A1L3KGU5|A0A1L3KGU5_9VIRU Capsid protein beta OS=Hubei unio douglasiae virus 1 OX=1923321 PE=4 SV=1
MM1 pKa = 7.65 DD2 pKa = 4.36 QFIEE6 pKa = 4.56 CYY8 pKa = 9.74 YY9 pKa = 10.35 IEE11 pKa = 6.13 LIFCTIILYY20 pKa = 10.23 LVLVLQRR27 pKa = 11.84 VKK29 pKa = 10.92 EE30 pKa = 4.23 KK31 pKa = 10.65 ILASHH36 pKa = 6.49 WNKK39 pKa = 10.15 KK40 pKa = 7.17 KK41 pKa = 10.43 QRR43 pKa = 11.84 ALTAQHH49 pKa = 6.3 ISTRR53 pKa = 11.84 VVRR56 pKa = 11.84 YY57 pKa = 9.46 ASATLRR63 pKa = 11.84 TRR65 pKa = 11.84 FQRR68 pKa = 11.84 QMEE71 pKa = 4.32 RR72 pKa = 11.84 VHH74 pKa = 7.31 SSVRR78 pKa = 11.84 VGHH81 pKa = 5.49 SHH83 pKa = 6.15 QSAASEE89 pKa = 4.14 RR90 pKa = 11.84 NSATEE95 pKa = 3.5 TMLAVVRR102 pKa = 11.84 KK103 pKa = 9.77 LGMIPYY109 pKa = 9.33 VISPSPRR116 pKa = 11.84 EE117 pKa = 3.85 KK118 pKa = 10.92 DD119 pKa = 3.07 CDD121 pKa = 4.29 GIRR124 pKa = 11.84 DD125 pKa = 4.21 YY126 pKa = 10.85 YY127 pKa = 10.51 TLADD131 pKa = 4.59 LRR133 pKa = 11.84 CQTKK137 pKa = 10.45 NDD139 pKa = 5.17 PITDD143 pKa = 3.27 NHH145 pKa = 6.88 VIIMTDD151 pKa = 2.23 VDD153 pKa = 4.41 YY154 pKa = 11.05 YY155 pKa = 11.87 VNMDD159 pKa = 4.48 EE160 pKa = 4.65 ILSHH164 pKa = 6.05 GRR166 pKa = 11.84 PVLLYY171 pKa = 8.36 TFQPTKK177 pKa = 10.51 VAGPVKK183 pKa = 10.41 NGYY186 pKa = 7.72 FTIEE190 pKa = 4.31 HH191 pKa = 6.47 DD192 pKa = 3.57 TVTYY196 pKa = 9.76 HH197 pKa = 6.38 VKK199 pKa = 10.18 GGKK202 pKa = 8.57 DD203 pKa = 3.28 VKK205 pKa = 10.8 HH206 pKa = 6.48 KK207 pKa = 9.64 IWNYY211 pKa = 10.44 SVDD214 pKa = 3.76 TIYY217 pKa = 10.87 TPNIVSGWFEE227 pKa = 4.4 TYY229 pKa = 10.16 FYY231 pKa = 10.85 AITDD235 pKa = 3.79 TLGEE239 pKa = 4.27 LTPIDD244 pKa = 4.07 GFFGLLSGLGKK255 pKa = 8.93 LTPFHH260 pKa = 7.19 KK261 pKa = 10.48 EE262 pKa = 3.44 INNFLYY268 pKa = 9.19 QTVTVSTIDD277 pKa = 3.12 QFYY280 pKa = 10.39 LSEE283 pKa = 4.45 HH284 pKa = 5.66 RR285 pKa = 11.84 TITTIVPYY293 pKa = 10.23 ARR295 pKa = 11.84 CPTDD299 pKa = 4.6 IIDD302 pKa = 4.05 TLAPATRR309 pKa = 11.84 LVRR312 pKa = 11.84 MNYY315 pKa = 8.98 QVPDD319 pKa = 3.66 EE320 pKa = 4.35 DD321 pKa = 5.45 HH322 pKa = 7.21 IEE324 pKa = 4.28 PKK326 pKa = 10.34 NDD328 pKa = 2.91 LRR330 pKa = 11.84 FNCLVHH336 pKa = 8.51 ISDD339 pKa = 4.11 SGPNISVGIAGEE351 pKa = 4.18 LAQATLPLSLFEE363 pKa = 4.58 SLRR366 pKa = 11.84 TAHH369 pKa = 6.63 SLAKK373 pKa = 10.31 ARR375 pKa = 11.84 ALSDD379 pKa = 3.18 TVRR382 pKa = 11.84 RR383 pKa = 11.84 SKK385 pKa = 11.21 LDD387 pKa = 3.56 NNEE390 pKa = 4.03 SATLHH395 pKa = 6.2 CFLTGVQKK403 pKa = 9.37 STPDD407 pKa = 3.19 IVHH410 pKa = 6.14 SPGLFSRR417 pKa = 11.84 HH418 pKa = 5.42 FNSVDD423 pKa = 3.49 QQDD426 pKa = 3.53 DD427 pKa = 4.29 TIPYY431 pKa = 10.57 DD432 pKa = 3.33 NGKK435 pKa = 10.39 EE436 pKa = 4.05 YY437 pKa = 10.99 ARR439 pKa = 11.84 DD440 pKa = 3.68 YY441 pKa = 11.65 APGPLSDD448 pKa = 3.36 TAVFPTEE455 pKa = 4.07 SSNNDD460 pKa = 2.85 KK461 pKa = 10.33 ATIRR465 pKa = 11.84 GRR467 pKa = 11.84 IDD469 pKa = 2.87 IPQRR473 pKa = 11.84 IANGTQYY480 pKa = 10.81 IKK482 pKa = 10.73 PQFLKK487 pKa = 10.33 FAHH490 pKa = 6.29 EE491 pKa = 4.3 FVSIILKK498 pKa = 8.57 NTTEE502 pKa = 4.4 RR503 pKa = 11.84 LGQPYY508 pKa = 10.69 SNDD511 pKa = 3.24 YY512 pKa = 11.02 VIEE515 pKa = 4.21 KK516 pKa = 9.87 QNKK519 pKa = 6.96 PRR521 pKa = 11.84 QRR523 pKa = 11.84 QRR525 pKa = 11.84 NDD527 pKa = 2.57 QGLFHH532 pKa = 7.38 VIDD535 pKa = 3.92 KK536 pKa = 10.1 FVVRR540 pKa = 11.84 AFQKK544 pKa = 10.26 RR545 pKa = 11.84 EE546 pKa = 3.82 AYY548 pKa = 10.23 NGPNYY553 pKa = 9.21 PRR555 pKa = 11.84 NISTVPGYY563 pKa = 10.99 HH564 pKa = 5.35 NTRR567 pKa = 11.84 LSGFTYY573 pKa = 10.42 AFKK576 pKa = 10.82 DD577 pKa = 3.44 AVLRR581 pKa = 11.84 THH583 pKa = 7.41 DD584 pKa = 3.54 WYY586 pKa = 11.1 QPCRR590 pKa = 11.84 TPEE593 pKa = 3.97 EE594 pKa = 3.75 IAANVHH600 pKa = 6.37 ALAQKK605 pKa = 10.47 HH606 pKa = 5.46 EE607 pKa = 4.3 YY608 pKa = 10.19 LVEE611 pKa = 3.87 TDD613 pKa = 3.42 YY614 pKa = 11.89 SKK616 pKa = 11.44 FDD618 pKa = 3.42 GTVTRR623 pKa = 11.84 WIRR626 pKa = 11.84 EE627 pKa = 3.97 NVEE630 pKa = 4.24 FPCYY634 pKa = 9.93 LRR636 pKa = 11.84 WVAPEE641 pKa = 4.57 HH642 pKa = 6.81 RR643 pKa = 11.84 DD644 pKa = 3.58 EE645 pKa = 5.99 LNTLLLSEE653 pKa = 5.12 LDD655 pKa = 4.16 APGTTNTISYY665 pKa = 10.75 SPGCSRR671 pKa = 11.84 LSGSPLTTDD680 pKa = 4.26 GNTICNAFVSYY691 pKa = 10.51 CAARR695 pKa = 11.84 SMKK698 pKa = 9.13 YY699 pKa = 8.7 TEE701 pKa = 5.2 AEE703 pKa = 3.97 AFSDD707 pKa = 4.13 IGLCYY712 pKa = 10.5 GDD714 pKa = 4.55 DD715 pKa = 3.81 GLRR718 pKa = 11.84 GGNVANTKK726 pKa = 9.33 LRR728 pKa = 11.84 KK729 pKa = 8.5 IASRR733 pKa = 11.84 LGFDD737 pKa = 4.45 LRR739 pKa = 11.84 VCNRR743 pKa = 11.84 AKK745 pKa = 10.61 AGSPVSFLSRR755 pKa = 11.84 VFADD759 pKa = 3.75 PWTSPASIQSPLRR772 pKa = 11.84 TLLKK776 pKa = 10.05 IHH778 pKa = 5.65 TTVDD782 pKa = 2.95 RR783 pKa = 11.84 RR784 pKa = 11.84 TKK786 pKa = 10.64 LSVVGAAKK794 pKa = 7.23 TTAYY798 pKa = 10.67 LVTDD802 pKa = 3.28 SRR804 pKa = 11.84 TPFISDD810 pKa = 3.01 WCRR813 pKa = 11.84 AYY815 pKa = 10.7 QKK817 pKa = 10.45 AAKK820 pKa = 8.52 AQPVANFNDD829 pKa = 3.94 LDD831 pKa = 5.33 DD832 pKa = 4.1 IPFWVLDD839 pKa = 3.99 TDD841 pKa = 4.27 NLAHH845 pKa = 7.34 PWPQDD850 pKa = 3.36 DD851 pKa = 4.55 PEE853 pKa = 5.82 NYY855 pKa = 8.66 MDD857 pKa = 4.99 IVATEE862 pKa = 4.16 LGITASEE869 pKa = 4.08 LSDD872 pKa = 4.21 HH873 pKa = 6.95 IKK875 pKa = 10.75 LLNEE879 pKa = 3.86 YY880 pKa = 9.89 SGDD883 pKa = 3.77 VAGLPVLQTTIDD895 pKa = 3.72 QTPKK899 pKa = 10.42 LAVVLDD905 pKa = 4.17 GEE907 pKa = 4.48 IHH909 pKa = 7.21 AGPNLQDD916 pKa = 4.27 DD917 pKa = 4.54 SNKK920 pKa = 10.46 HH921 pKa = 5.05 GIARR925 pKa = 11.84 QDD927 pKa = 3.29 VPVAQHH933 pKa = 5.95 RR934 pKa = 11.84 RR935 pKa = 11.84 SGAVEE940 pKa = 3.8 RR941 pKa = 11.84 EE942 pKa = 3.66 NRR944 pKa = 11.84 ARR946 pKa = 11.84 PPHH949 pKa = 6.49 VSTAPRR955 pKa = 11.84 RR956 pKa = 11.84 GPQSNYY962 pKa = 9.43 PRR964 pKa = 11.84 GSTNPHH970 pKa = 6.51 KK971 pKa = 10.44 DD972 pKa = 3.11 SKK974 pKa = 11.13 EE975 pKa = 3.82 RR976 pKa = 11.84 TDD978 pKa = 3.71 PVSSKK983 pKa = 10.58 HH984 pKa = 6.08 RR985 pKa = 11.84 GADD988 pKa = 3.04 QTHH991 pKa = 6.61 PPKK994 pKa = 10.86 CEE996 pKa = 3.73 ANGRR1000 pKa = 11.84 RR1001 pKa = 11.84 SGKK1004 pKa = 7.94 TRR1006 pKa = 11.84 GRR1008 pKa = 11.84 TGKK1011 pKa = 10.61 SNAATDD1017 pKa = 3.78 QSNHH1021 pKa = 4.71 GSAAGGRR1028 pKa = 11.84 QSKK1031 pKa = 7.98 ITQGLAKK1038 pKa = 9.75 TDD1040 pKa = 3.31
Molecular weight: 116.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.342
IPC2_protein 7.732
IPC_protein 7.541
Toseland 7.337
ProMoST 8.112
Dawson 8.273
Bjellqvist 8.595
Wikipedia 8.214
Rodwell 8.302
Grimsley 7.454
Solomon 8.317
Lehninger 8.317
Nozaki 8.712
DTASelect 8.361
Thurlkill 8.404
EMBOSS 8.463
Sillero 8.697
Patrickios 4.317
IPC_peptide 8.302
IPC2_peptide 7.541
IPC2.peptide.svr19 7.672
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1544
154
1040
514.7
57.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.578 ± 0.697
1.619 ± 0.397
5.894 ± 1.041
4.275 ± 0.615
3.368 ± 0.711
6.412 ± 1.633
2.591 ± 0.989
5.829 ± 0.183
4.534 ± 0.613
8.355 ± 1.918
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.813 ± 1.081
4.793 ± 0.124
5.829 ± 0.453
4.016 ± 0.522
5.959 ± 1.129
7.124 ± 0.214
8.225 ± 0.081
6.736 ± 0.418
1.101 ± 0.156
3.951 ± 0.422
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here