bacterium HR41

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1586 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6B7P1|A0A2H6B7P1_9BACT Uncharacterized protein OS=bacterium HR41 OX=2035436 GN=HRbin41_01029 PE=4 SV=1
MM1 pKa = 7.69EE2 pKa = 4.28SHH4 pKa = 6.46EE5 pKa = 4.36LEE7 pKa = 4.38KK8 pKa = 10.17THH10 pKa = 7.03ADD12 pKa = 3.51SGGSAGDD19 pKa = 3.65PSAAAGDD26 pKa = 3.98GPPTVEE32 pKa = 4.35EE33 pKa = 3.99VRR35 pKa = 11.84EE36 pKa = 4.0ALTNVIDD43 pKa = 4.45PEE45 pKa = 5.32LGLDD49 pKa = 3.98FVEE52 pKa = 5.65LGLIYY57 pKa = 9.95DD58 pKa = 3.99IQVEE62 pKa = 4.2DD63 pKa = 3.85GDD65 pKa = 4.51VYY67 pKa = 10.49VTYY70 pKa = 10.2TLTSPGCPIGPHH82 pKa = 6.17IADD85 pKa = 3.55QIEE88 pKa = 4.5EE89 pKa = 4.41FVSEE93 pKa = 4.2LPGVQRR99 pKa = 11.84VYY101 pKa = 10.99PKK103 pKa = 10.11LTFDD107 pKa = 4.64PPWTPDD113 pKa = 3.22MMSEE117 pKa = 4.08DD118 pKa = 3.6AKK120 pKa = 10.95FALGYY125 pKa = 10.59

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6B883|A0A2H6B883_9BACT UPF0109 protein HRbin41_01221 OS=bacterium HR41 OX=2035436 GN=HRbin41_01221 PE=3 SV=1
MM1 pKa = 7.49GAPRR5 pKa = 11.84PPRR8 pKa = 11.84ATKK11 pKa = 10.57VPATTSRR18 pKa = 11.84APRR21 pKa = 11.84ATPQLAAALPAPRR34 pKa = 11.84AAKK37 pKa = 10.36LGATARR43 pKa = 11.84RR44 pKa = 11.84STSSCRR50 pKa = 11.84RR51 pKa = 11.84WASRR55 pKa = 11.84CKK57 pKa = 10.0RR58 pKa = 11.84APSSSGSSASATRR71 pKa = 11.84SARR74 pKa = 11.84DD75 pKa = 3.32SRR77 pKa = 11.84SSRR80 pKa = 11.84FRR82 pKa = 11.84PIRR85 pKa = 11.84STPRR89 pKa = 11.84SPHH92 pKa = 6.69PPTEE96 pKa = 4.05PSPRR100 pKa = 11.84SSSRR104 pKa = 11.84ATRR107 pKa = 11.84RR108 pKa = 11.84SPSVPSSAGSQRR120 pKa = 11.84VRR122 pKa = 11.84RR123 pKa = 11.84RR124 pKa = 11.84RR125 pKa = 11.84RR126 pKa = 11.84SSRR129 pKa = 11.84ATLAVKK135 pKa = 9.83DD136 pKa = 3.7APRR139 pKa = 11.84RR140 pKa = 11.84RR141 pKa = 11.84SRR143 pKa = 11.84VLRR146 pKa = 11.84VGRR149 pKa = 11.84RR150 pKa = 11.84PGPRR154 pKa = 11.84LSRR157 pKa = 11.84RR158 pKa = 11.84AAPGSATGLQRR169 pKa = 11.84RR170 pKa = 11.84PSLAVRR176 pKa = 11.84RR177 pKa = 11.84RR178 pKa = 11.84LTVSTSRR185 pKa = 11.84ACRR188 pKa = 11.84AAGPGVASPKK198 pKa = 9.32RR199 pKa = 11.84TSCVQQAARR208 pKa = 11.84APPAAMAQQPTALATPAAPAWLPLARR234 pKa = 11.84RR235 pKa = 11.84ALRR238 pKa = 11.84RR239 pKa = 11.84HH240 pKa = 5.91RR241 pKa = 11.84PFGRR245 pKa = 11.84CRR247 pKa = 11.84CADD250 pKa = 3.42RR251 pKa = 11.84RR252 pKa = 11.84RR253 pKa = 11.84RR254 pKa = 11.84WPATWSAAARR264 pKa = 11.84SRR266 pKa = 11.84RR267 pKa = 11.84RR268 pKa = 11.84RR269 pKa = 11.84ASARR273 pKa = 11.84SRR275 pKa = 11.84STSSTGAGASSRR287 pKa = 11.84PPANVCRR294 pKa = 11.84SLTT297 pKa = 3.51

Molecular weight: 31.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1586

0

1586

457819

29

1209

288.7

31.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.74 ± 0.107

1.027 ± 0.019

5.284 ± 0.051

6.742 ± 0.078

2.88 ± 0.034

8.816 ± 0.059

1.97 ± 0.032

3.38 ± 0.045

1.661 ± 0.037

10.633 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.347 ± 0.024

1.537 ± 0.025

5.763 ± 0.044

2.55 ± 0.033

10.832 ± 0.074

4.61 ± 0.042

4.528 ± 0.037

8.758 ± 0.058

1.176 ± 0.026

1.768 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski