Fusarium poae partitivirus 2
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B4ZA58|A0A1B4ZA58_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae partitivirus 2 OX=1849533 PE=4 SV=1
MM1 pKa = 7.6 HH2 pKa = 7.35 SLFTIVNLLLLARR15 pKa = 11.84 FQLRR19 pKa = 11.84 RR20 pKa = 11.84 KK21 pKa = 9.87 RR22 pKa = 11.84 KK23 pKa = 7.34 EE24 pKa = 3.81 TTKK27 pKa = 10.47 TLIYY31 pKa = 9.47 PIRR34 pKa = 11.84 NILFEE39 pKa = 4.17 WEE41 pKa = 4.35 LRR43 pKa = 11.84 RR44 pKa = 11.84 STPRR48 pKa = 11.84 EE49 pKa = 3.3 PGIPLRR55 pKa = 11.84 YY56 pKa = 8.96 FDD58 pKa = 4.14 YY59 pKa = 11.02 SKK61 pKa = 11.51 SLLSYY66 pKa = 10.64 IDD68 pKa = 4.4 LQTCHH73 pKa = 7.4 KK74 pKa = 10.47 INIHH78 pKa = 6.81 LDD80 pKa = 3.41 DD81 pKa = 4.32 HH82 pKa = 6.68 TDD84 pKa = 3.3 ALMEE88 pKa = 4.63 RR89 pKa = 11.84 YY90 pKa = 9.52 ASRR93 pKa = 11.84 DD94 pKa = 3.34 EE95 pKa = 4.25 PFAVYY100 pKa = 8.18 QHH102 pKa = 6.79 ISDD105 pKa = 4.03 EE106 pKa = 4.26 DD107 pKa = 3.9 LPPEE111 pKa = 4.16 RR112 pKa = 11.84 TPAPGIRR119 pKa = 11.84 HH120 pKa = 5.26 AQCRR124 pKa = 11.84 YY125 pKa = 9.84 HH126 pKa = 7.19 EE127 pKa = 4.85 IPSGTLNLDD136 pKa = 3.59 EE137 pKa = 4.83 KK138 pKa = 11.05 QHH140 pKa = 5.88 VLTDD144 pKa = 3.83 DD145 pKa = 3.95 PDD147 pKa = 4.94 FIEE150 pKa = 4.5 TPEE153 pKa = 3.87 FRR155 pKa = 11.84 SGILYY160 pKa = 10.5 DD161 pKa = 3.57 EE162 pKa = 5.26 TIDD165 pKa = 4.84 LSGSPPIPEE174 pKa = 3.93 IAQIIHH180 pKa = 6.79 DD181 pKa = 4.69 WFPHH185 pKa = 5.58 FEE187 pKa = 4.23 PFLAEE192 pKa = 3.88 YY193 pKa = 10.11 CRR195 pKa = 11.84 PPSFGPQAFRR205 pKa = 11.84 DD206 pKa = 4.41 FNRR209 pKa = 11.84 PTPHH213 pKa = 7.33 PPPPPHH219 pKa = 6.54 EE220 pKa = 3.91 RR221 pKa = 11.84 HH222 pKa = 5.4 EE223 pKa = 4.93 AIMDD227 pKa = 3.77 IVRR230 pKa = 11.84 AKK232 pKa = 10.78 FNLKK236 pKa = 9.74 PYY238 pKa = 10.63 RR239 pKa = 11.84 PMHH242 pKa = 5.82 YY243 pKa = 10.18 VDD245 pKa = 5.27 ALAAEE250 pKa = 4.78 TPLNTSASYY259 pKa = 10.64 YY260 pKa = 10.8 SKK262 pKa = 10.86 FNPTSRR268 pKa = 11.84 VFARR272 pKa = 11.84 YY273 pKa = 8.47 SAPSRR278 pKa = 11.84 YY279 pKa = 9.18 KK280 pKa = 10.73 DD281 pKa = 3.14 KK282 pKa = 9.64 PTSKK286 pKa = 10.51 GYY288 pKa = 10.43 NFNVVMNEE296 pKa = 3.2 FRR298 pKa = 11.84 TEE300 pKa = 3.89 YY301 pKa = 10.74 HH302 pKa = 6.28 HH303 pKa = 7.03 IKK305 pKa = 10.27 YY306 pKa = 10.55 DD307 pKa = 3.85 GVPFPADD314 pKa = 3.26 LHH316 pKa = 7.12 DD317 pKa = 4.83 PEE319 pKa = 6.56 ANASILNTWFAKK331 pKa = 10.45 HH332 pKa = 6.21 PSQLFIRR339 pKa = 11.84 TQISKK344 pKa = 10.39 RR345 pKa = 11.84 DD346 pKa = 3.56 PNDD349 pKa = 3.29 PKK351 pKa = 10.68 KK352 pKa = 10.3 IRR354 pKa = 11.84 PVYY357 pKa = 10.59 SVDD360 pKa = 4.16 DD361 pKa = 3.94 RR362 pKa = 11.84 FLHH365 pKa = 6.0 IEE367 pKa = 3.85 KK368 pKa = 9.55 TLVVPALAQLRR379 pKa = 11.84 NPQCCVAHH387 pKa = 5.99 GLEE390 pKa = 4.28 TFRR393 pKa = 11.84 GSMSLLDD400 pKa = 3.72 RR401 pKa = 11.84 TALVYY406 pKa = 9.82 TSYY409 pKa = 11.21 ISLDD413 pKa = 2.82 WSQFDD418 pKa = 3.23 QRR420 pKa = 11.84 LPYY423 pKa = 10.14 YY424 pKa = 10.23 VIIAFFLDD432 pKa = 4.22 FLPSLLIISHH442 pKa = 6.85 GYY444 pKa = 8.72 FPSRR448 pKa = 11.84 GYY450 pKa = 11.0 EE451 pKa = 3.86 STPQDD456 pKa = 2.6 IHH458 pKa = 8.44 AFASKK463 pKa = 10.12 IFNVVLFLTTWYY475 pKa = 11.09 LNMTFVSFDD484 pKa = 2.78 GFAYY488 pKa = 9.5 IRR490 pKa = 11.84 EE491 pKa = 4.38 HH492 pKa = 6.76 GGVPSGLLNTQFLDD506 pKa = 3.54 SFGNMYY512 pKa = 10.37 IIVDD516 pKa = 3.9 CLLEE520 pKa = 4.33 FGFTPAEE527 pKa = 4.24 CLDD530 pKa = 3.53 MLYY533 pKa = 10.72 CVLGDD538 pKa = 4.24 DD539 pKa = 3.97 NLIFARR545 pKa = 11.84 QNFDD549 pKa = 4.17 RR550 pKa = 11.84 ICDD553 pKa = 3.63 FMIFLTKK560 pKa = 10.68 YY561 pKa = 9.37 ADD563 pKa = 3.58 TRR565 pKa = 11.84 HH566 pKa = 5.29 GMVVSILKK574 pKa = 10.03 SVYY577 pKa = 8.7 TKK579 pKa = 10.66 LRR581 pKa = 11.84 SKK583 pKa = 10.56 ISFLSYY589 pKa = 10.13 EE590 pKa = 4.16 NTYY593 pKa = 11.01 GMPTRR598 pKa = 11.84 PIGKK602 pKa = 9.14 LVAQLSMPEE611 pKa = 4.23 RR612 pKa = 11.84 PIPDD616 pKa = 3.07 NRR618 pKa = 11.84 KK619 pKa = 7.99 WIHH622 pKa = 5.8 AARR625 pKa = 11.84 ALGLAYY631 pKa = 10.26 ANCGQDD637 pKa = 2.95 AHH639 pKa = 6.34 FHH641 pKa = 6.23 LLCHH645 pKa = 5.45 MVYY648 pKa = 10.67 EE649 pKa = 4.65 KK650 pKa = 10.61 FRR652 pKa = 11.84 PDD654 pKa = 3.12 TPVPSLHH661 pKa = 6.37 IEE663 pKa = 4.07 KK664 pKa = 10.35 VFKK667 pKa = 9.77 KK668 pKa = 9.46 WKK670 pKa = 8.01 YY671 pKa = 8.86 QLPEE675 pKa = 3.77 FDD677 pKa = 4.58 IEE679 pKa = 4.69 SVTYY683 pKa = 7.87 TFPDD687 pKa = 4.6 FPTLFSIRR695 pKa = 11.84 QLVSDD700 pKa = 3.27 YY701 pKa = 11.28 HH702 pKa = 8.1 GFFSEE707 pKa = 4.09 TDD709 pKa = 2.78 KK710 pKa = 11.36 WNTDD714 pKa = 3.08 MFEE717 pKa = 4.8 APPSDD722 pKa = 3.73 NLHH725 pKa = 7.29 DD726 pKa = 3.93 YY727 pKa = 8.88 VTLKK731 pKa = 10.87 EE732 pKa = 4.06 YY733 pKa = 10.59 MNSNVHH739 pKa = 5.7 MSHH742 pKa = 6.21 TVNEE746 pKa = 4.27 FMHH749 pKa = 6.71 GKK751 pKa = 10.12 RR752 pKa = 11.84 SFLL755 pKa = 3.74
Molecular weight: 88.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.059
IPC2_protein 6.008
IPC_protein 6.122
Toseland 6.561
ProMoST 6.532
Dawson 6.453
Bjellqvist 6.44
Wikipedia 6.491
Rodwell 6.453
Grimsley 6.751
Solomon 6.453
Lehninger 6.453
Nozaki 6.751
DTASelect 6.927
Thurlkill 6.956
EMBOSS 6.927
Sillero 6.854
Patrickios 3.884
IPC_peptide 6.478
IPC2_peptide 6.795
IPC2.peptide.svr19 6.74
Protein with the highest isoelectric point:
>tr|A0A1B4ZA58|A0A1B4ZA58_9VIRU RNA-dependent RNA polymerase OS=Fusarium poae partitivirus 2 OX=1849533 PE=4 SV=1
MM1 pKa = 7.37 SRR3 pKa = 11.84 FGPRR7 pKa = 11.84 TTIPAPPPPEE17 pKa = 4.98 PIQAATDD24 pKa = 4.16 PNTPAPPNPLHH35 pKa = 7.71 DD36 pKa = 3.29 ITQAINAPGNVTSNEE51 pKa = 3.56 GDD53 pKa = 3.88 LFILDD58 pKa = 4.15 TVPDD62 pKa = 3.64 HH63 pKa = 7.44 RR64 pKa = 11.84 FPLLSWLYY72 pKa = 10.05 SIPRR76 pKa = 11.84 VLQPTAYY83 pKa = 9.8 SQAPNASPASLLGYY97 pKa = 8.57 TYY99 pKa = 11.51 LMYY102 pKa = 10.5 IGLLFHH108 pKa = 7.16 NDD110 pKa = 2.42 AFQRR114 pKa = 11.84 PSPSKK119 pKa = 9.21 HH120 pKa = 5.52 ARR122 pKa = 11.84 SILSDD127 pKa = 3.73 YY128 pKa = 11.2 YY129 pKa = 11.3 LATFFNSLLDD139 pKa = 3.83 LPVPAFAALDD149 pKa = 3.88 FEE151 pKa = 4.61 ALRR154 pKa = 11.84 FFNHH158 pKa = 6.88 DD159 pKa = 3.45 LASNLVAIGSEE170 pKa = 3.86 AGFSFAHH177 pKa = 6.51 DD178 pKa = 4.36 FGRR181 pKa = 11.84 FFTAGTFFGLHH192 pKa = 5.35 ATLATMPGNTQLAAAQLAFSKK213 pKa = 8.16 MTVARR218 pKa = 11.84 ITTAPGQTVNLAPGHH233 pKa = 6.24 LFGTVMNDD241 pKa = 3.11 TPYY244 pKa = 11.29 LNWLNTRR251 pKa = 11.84 LSAILNQSEE260 pKa = 4.21 IRR262 pKa = 11.84 SMASRR267 pKa = 11.84 PNAGRR272 pKa = 11.84 IPMFAIPEE280 pKa = 4.03 QEE282 pKa = 3.91 MSDD285 pKa = 3.73 VNPYY289 pKa = 10.79 LLMMSISDD297 pKa = 4.66 DD298 pKa = 3.65 NLEE301 pKa = 5.23 LLTSWIRR308 pKa = 11.84 NLASFTRR315 pKa = 11.84 EE316 pKa = 3.51 TLTTSRR322 pKa = 11.84 PLRR325 pKa = 11.84 NYY327 pKa = 7.06 TQPGNPEE334 pKa = 4.0 VLRR337 pKa = 11.84 HH338 pKa = 5.73 LVFEE342 pKa = 4.75 APPPTWHH349 pKa = 6.77 SDD351 pKa = 3.09 SLGTHH356 pKa = 6.45 TSLNPTPEE364 pKa = 4.02 PQNPFRR370 pKa = 11.84 VGNHH374 pKa = 5.58 PHH376 pKa = 5.92 THH378 pKa = 4.8 TQFAAARR385 pKa = 11.84 NFRR388 pKa = 11.84 TRR390 pKa = 11.84 SGIPAPIATNNPLAANAAAPPSVDD414 pKa = 3.29 NSWFASLVSTNEE426 pKa = 3.64 NAHH429 pKa = 6.41 PNDD432 pKa = 3.36 PVVRR436 pKa = 11.84 RR437 pKa = 11.84 RR438 pKa = 11.84 LTADD442 pKa = 2.91 GHH444 pKa = 5.81 FANTPRR450 pKa = 11.84 AVIFEE455 pKa = 4.34 PTSATEE461 pKa = 4.1 STAHH465 pKa = 5.81 HH466 pKa = 6.01 VAVITSGKK474 pKa = 9.64 IIEE477 pKa = 4.87 SGDD480 pKa = 3.37 FTGTVIPTVHH490 pKa = 6.96 PRR492 pKa = 11.84 RR493 pKa = 11.84 NLYY496 pKa = 9.46 LQNTHH501 pKa = 5.89 YY502 pKa = 11.02 LSGAVLISQTRR513 pKa = 11.84 PAIRR517 pKa = 11.84 NEE519 pKa = 3.59 YY520 pKa = 10.28 FDD522 pKa = 3.44 IHH524 pKa = 6.52 NVTDD528 pKa = 3.67 EE529 pKa = 4.48 DD530 pKa = 4.55 LLRR533 pKa = 11.84 SPLGILRR540 pKa = 11.84 GYY542 pKa = 9.58 SSRR545 pKa = 11.84 LRR547 pKa = 11.84 LPLFRR552 pKa = 11.84 QGIVEE557 pKa = 4.01 RR558 pKa = 11.84 ALTFEE563 pKa = 4.32 SANFSSLIASGATLLPNAHH582 pKa = 7.03 RR583 pKa = 11.84 AFDD586 pKa = 3.66 STNVFLAPDD595 pKa = 3.95 NQGHH599 pKa = 6.5 LVDD602 pKa = 5.59 DD603 pKa = 5.43 EE604 pKa = 4.52 IWPIWSSYY612 pKa = 7.22 RR613 pKa = 11.84 HH614 pKa = 5.48 YY615 pKa = 10.82 NQEE618 pKa = 3.26 ARR620 pKa = 11.84 QWYY623 pKa = 7.93 VLPSLRR629 pKa = 11.84 HH630 pKa = 4.9 IFGTQARR637 pKa = 11.84 TFLTDD642 pKa = 3.09 HH643 pKa = 7.27 PSRR646 pKa = 11.84 RR647 pKa = 11.84 IPAA650 pKa = 3.83
Molecular weight: 71.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.501
IPC2_protein 6.249
IPC_protein 6.415
Toseland 7.073
ProMoST 6.825
Dawson 6.751
Bjellqvist 6.707
Wikipedia 6.781
Rodwell 6.751
Grimsley 7.307
Solomon 6.751
Lehninger 6.751
Nozaki 7.015
DTASelect 7.234
Thurlkill 7.263
EMBOSS 7.234
Sillero 7.132
Patrickios 4.355
IPC_peptide 6.766
IPC2_peptide 7.117
IPC2.peptide.svr19 6.955
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1405
650
755
702.5
79.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.616 ± 1.721
0.783 ± 0.5
5.552 ± 0.795
4.27 ± 0.566
6.619 ± 0.494
3.986 ± 0.402
4.27 ± 0.173
5.694 ± 0.394
2.562 ± 1.343
9.751 ± 0.258
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.993 ± 0.29
5.125 ± 0.953
8.754 ± 0.501
2.847 ± 0.147
6.335 ± 0.18
7.331 ± 0.428
6.975 ± 0.95
4.342 ± 0.12
1.139 ± 0.059
4.057 ± 0.922
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here