Aquisalibacillus elongatus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Aquisalibacillus

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2958 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N5CDP6|A0A3N5CDP6_9BACI Aldehyde dehydrogenase (Acceptor) OS=Aquisalibacillus elongatus OX=485577 GN=EDC24_0122 PE=3 SV=1
MM1 pKa = 7.39SKK3 pKa = 10.68SLFGKK8 pKa = 10.08FLFVLMLAFVLLIAACNSDD27 pKa = 3.3EE28 pKa = 4.29GGEE31 pKa = 4.08DD32 pKa = 3.9SSDD35 pKa = 3.43NDD37 pKa = 5.12GDD39 pKa = 3.71DD40 pKa = 3.86TEE42 pKa = 5.04QNEE45 pKa = 4.7SDD47 pKa = 5.35DD48 pKa = 5.04DD49 pKa = 5.46DD50 pKa = 6.08SDD52 pKa = 4.29SDD54 pKa = 3.93SDD56 pKa = 4.3SDD58 pKa = 4.08QEE60 pKa = 4.92LYY62 pKa = 11.06SIDD65 pKa = 4.54DD66 pKa = 3.82FEE68 pKa = 6.46SYY70 pKa = 9.79TGEE73 pKa = 4.0GDD75 pKa = 4.32AIDD78 pKa = 4.54GGSLTFGLVSQSPFEE93 pKa = 4.34GTLNWQFYY101 pKa = 10.42SGNPDD106 pKa = 3.39SQVLSWFDD114 pKa = 3.67EE115 pKa = 4.15SLLQYY120 pKa = 10.79DD121 pKa = 3.66EE122 pKa = 4.93NYY124 pKa = 10.97NVTNEE129 pKa = 3.82GAASYY134 pKa = 9.66EE135 pKa = 4.19VNEE138 pKa = 4.47EE139 pKa = 4.01DD140 pKa = 4.53SSITFTIEE148 pKa = 5.8DD149 pKa = 3.7DD150 pKa = 4.51VKK152 pKa = 9.47WHH154 pKa = 7.13DD155 pKa = 3.9GEE157 pKa = 4.63EE158 pKa = 4.22VTAEE162 pKa = 3.79DD163 pKa = 2.96WAYY166 pKa = 9.07SYY168 pKa = 10.94EE169 pKa = 4.56VIMHH173 pKa = 7.09PDD175 pKa = 3.17YY176 pKa = 11.6DD177 pKa = 4.06GVRR180 pKa = 11.84GSSAGFAYY188 pKa = 10.74LEE190 pKa = 4.47GAQEE194 pKa = 4.17YY195 pKa = 10.0KK196 pKa = 10.74AGEE199 pKa = 4.16ADD201 pKa = 3.94SISGIEE207 pKa = 3.97IEE209 pKa = 4.38GEE211 pKa = 3.77KK212 pKa = 10.23TITFNYY218 pKa = 9.54TEE220 pKa = 4.64LTPSLLTGGVWGYY233 pKa = 10.41PMAKK237 pKa = 9.57HH238 pKa = 5.58VFEE241 pKa = 6.08DD242 pKa = 3.92IPVEE246 pKa = 4.24DD247 pKa = 4.18MASSPEE253 pKa = 3.95VRR255 pKa = 11.84EE256 pKa = 3.99EE257 pKa = 4.33PIGFGPYY264 pKa = 9.31KK265 pKa = 10.41VEE267 pKa = 3.79NVVPGEE273 pKa = 4.07SVTYY277 pKa = 10.73SKK279 pKa = 11.3FEE281 pKa = 4.61DD282 pKa = 3.82YY283 pKa = 10.28WRR285 pKa = 11.84GEE287 pKa = 4.06PNLDD291 pKa = 3.25EE292 pKa = 4.37VTLKK296 pKa = 10.7VVSPSNVANALEE308 pKa = 4.4TGDD311 pKa = 3.83VDD313 pKa = 5.66LVDD316 pKa = 5.12SFPADD321 pKa = 3.16QVVDD325 pKa = 4.41NIDD328 pKa = 3.55MPNVQWLGMTDD339 pKa = 3.59LAYY342 pKa = 9.64TYY344 pKa = 10.46IGFKK348 pKa = 10.63LGDD351 pKa = 3.36WNADD355 pKa = 3.41EE356 pKa = 6.22GEE358 pKa = 4.27VNYY361 pKa = 10.89KK362 pKa = 9.83GDD364 pKa = 3.76EE365 pKa = 3.99MKK367 pKa = 10.54MGDD370 pKa = 3.45KK371 pKa = 10.46KK372 pKa = 10.76LRR374 pKa = 11.84KK375 pKa = 9.43AMWYY379 pKa = 10.12AVDD382 pKa = 3.76NEE384 pKa = 4.83TVGEE388 pKa = 4.03RR389 pKa = 11.84FYY391 pKa = 11.8NGLRR395 pKa = 11.84WNGTTLIPPSHH406 pKa = 6.93PAWHH410 pKa = 7.62DD411 pKa = 3.59DD412 pKa = 3.91SIEE415 pKa = 4.12TPTYY419 pKa = 11.03DD420 pKa = 3.32EE421 pKa = 5.35DD422 pKa = 4.35KK423 pKa = 11.33ANEE426 pKa = 4.09LLDD429 pKa = 4.01DD430 pKa = 5.02AGYY433 pKa = 11.18KK434 pKa = 8.79DD435 pKa = 4.26TNDD438 pKa = 3.23DD439 pKa = 4.23GYY441 pKa = 11.32RR442 pKa = 11.84EE443 pKa = 4.22TPDD446 pKa = 3.85GEE448 pKa = 3.97EE449 pKa = 3.83LVINFASMDD458 pKa = 3.74SSDD461 pKa = 4.53LSEE464 pKa = 4.54PLANYY469 pKa = 8.64YY470 pKa = 8.97IQAWDD475 pKa = 3.52KK476 pKa = 11.58VGLNVQKK483 pKa = 10.93LDD485 pKa = 3.75GRR487 pKa = 11.84LHH489 pKa = 6.38EE490 pKa = 5.13FNSFYY495 pKa = 11.28DD496 pKa = 3.53KK497 pKa = 11.09VGQTGEE503 pKa = 4.45DD504 pKa = 3.8DD505 pKa = 4.21PNVDD509 pKa = 3.14VFMGAWSVGYY519 pKa = 10.32DD520 pKa = 3.43VDD522 pKa = 4.11PSGLYY527 pKa = 10.68GRR529 pKa = 11.84DD530 pKa = 2.84ALFNFTRR537 pKa = 11.84WANEE541 pKa = 3.78EE542 pKa = 3.85NDD544 pKa = 3.51EE545 pKa = 4.4LLAEE549 pKa = 4.61GLSEE553 pKa = 3.92EE554 pKa = 5.02AFDD557 pKa = 3.5TEE559 pKa = 4.21YY560 pKa = 11.1RR561 pKa = 11.84QDD563 pKa = 5.0VYY565 pKa = 11.22SQWQEE570 pKa = 3.7LMVEE574 pKa = 4.39EE575 pKa = 4.74VPVFPTLYY583 pKa = 9.46RR584 pKa = 11.84AMMVPANEE592 pKa = 3.76RR593 pKa = 11.84VVNYY597 pKa = 9.63DD598 pKa = 2.76ISVGSGVYY606 pKa = 10.09LYY608 pKa = 10.62EE609 pKa = 5.06LGVTQEE615 pKa = 4.21EE616 pKa = 4.86PVVEE620 pKa = 4.24

Molecular weight: 69.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N5BZQ6|A0A3N5BZQ6_9BACI CubicO group peptidase (Beta-lactamase class C family) OS=Aquisalibacillus elongatus OX=485577 GN=EDC24_0179 PE=4 SV=1
MM1 pKa = 7.52MKK3 pKa = 9.41RR4 pKa = 11.84TYY6 pKa = 10.32QPNKK10 pKa = 8.19RR11 pKa = 11.84KK12 pKa = 9.74RR13 pKa = 11.84NKK15 pKa = 8.71VHH17 pKa = 6.87GFRR20 pKa = 11.84TRR22 pKa = 11.84MKK24 pKa = 8.87TKK26 pKa = 10.27NGRR29 pKa = 11.84KK30 pKa = 8.66VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.2KK38 pKa = 10.05GRR40 pKa = 11.84KK41 pKa = 8.66VLSAA45 pKa = 4.05

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2958

0

2958

843163

29

2845

285.0

32.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.098 ± 0.049

0.58 ± 0.012

5.982 ± 0.042

8.122 ± 0.055

4.529 ± 0.041

6.599 ± 0.047

2.247 ± 0.021

7.762 ± 0.052

6.264 ± 0.041

9.585 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.768 ± 0.02

4.685 ± 0.036

3.481 ± 0.025

4.316 ± 0.039

3.903 ± 0.031

5.94 ± 0.039

5.243 ± 0.032

7.163 ± 0.04

0.997 ± 0.017

3.737 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski