Ruminococcaceae bacterium TF06-43

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; unclassified Oscillospiraceae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A417ND16|A0A417ND16_9FIRM Phosphatidic acid phosphatase OS=Ruminococcaceae bacterium TF06-43 OX=2292270 GN=DXC62_07160 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.41KK3 pKa = 10.59KK4 pKa = 10.24FMCMLMAAAMTLSMAACGSKK24 pKa = 10.42SDD26 pKa = 5.26DD27 pKa = 3.78TNTDD31 pKa = 3.46DD32 pKa = 4.49NAGGASDD39 pKa = 4.58GVQTFTVGTSGPLTGDD55 pKa = 3.18NAIYY59 pKa = 10.61GMAVKK64 pKa = 10.28QGVEE68 pKa = 3.78LAVNEE73 pKa = 4.34INASDD78 pKa = 3.67STIKK82 pKa = 10.81FEE84 pKa = 5.85FLSQDD89 pKa = 3.66DD90 pKa = 4.0EE91 pKa = 6.31ADD93 pKa = 3.5GEE95 pKa = 4.57KK96 pKa = 10.58AVNAYY101 pKa = 10.75NNMMDD106 pKa = 3.72NGMQVLVGPTTTGASIAVADD126 pKa = 3.61ACYY129 pKa = 10.06TDD131 pKa = 3.8RR132 pKa = 11.84TFMLTPSASSTDD144 pKa = 3.36VTAGKK149 pKa = 10.63DD150 pKa = 3.28NVFQVCFTDD159 pKa = 3.85PNQGVGAADD168 pKa = 3.3YY169 pKa = 9.06MAEE172 pKa = 4.02NFADD176 pKa = 3.53AKK178 pKa = 10.42IAVIYY183 pKa = 10.66RR184 pKa = 11.84NDD186 pKa = 3.46DD187 pKa = 3.57AYY189 pKa = 11.61SQGIRR194 pKa = 11.84DD195 pKa = 4.12AFVKK199 pKa = 10.43EE200 pKa = 4.33AGDD203 pKa = 3.52KK204 pKa = 10.78GLSIVYY210 pKa = 10.02QGTFTLDD217 pKa = 2.97TSSNFSVQLAAAQTAGADD235 pKa = 3.83LVFLPIYY242 pKa = 7.46YY243 pKa = 10.04QPASVIFAQAKK254 pKa = 9.03AMGYY258 pKa = 10.1APTFFGVDD266 pKa = 3.36GMDD269 pKa = 5.55GILDD273 pKa = 3.61MPGFDD278 pKa = 3.18TTLAEE283 pKa = 4.54GLVLMTPFCATVEE296 pKa = 4.26SSKK299 pKa = 11.29SFVDD303 pKa = 4.48AYY305 pKa = 8.85TAAYY309 pKa = 7.19GTTPNQFAADD319 pKa = 4.11AYY321 pKa = 10.39DD322 pKa = 3.07GVYY325 pKa = 9.66IVKK328 pKa = 10.28AALEE332 pKa = 4.18QAGCTADD339 pKa = 3.37MSNEE343 pKa = 4.19EE344 pKa = 3.87ICDD347 pKa = 3.81ALVSAITSLKK357 pKa = 9.31FTGLTGTDD365 pKa = 3.45MSWNAEE371 pKa = 4.09GQVSKK376 pKa = 11.26APTAYY381 pKa = 9.84VVKK384 pKa = 10.38DD385 pKa = 3.48GQYY388 pKa = 10.46VEE390 pKa = 4.63GG391 pKa = 4.4

Molecular weight: 40.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A417MZ85|A0A417MZ85_9FIRM ATPase (Fragment) OS=Ruminococcaceae bacterium TF06-43 OX=2292270 GN=DXC62_16635 PE=4 SV=1
MM1 pKa = 7.53PRR3 pKa = 11.84MSKK6 pKa = 9.9KK7 pKa = 10.0RR8 pKa = 11.84RR9 pKa = 11.84LEE11 pKa = 3.44WSFFLRR17 pKa = 11.84QVKK20 pKa = 9.89VGNTTCDD27 pKa = 3.22RR28 pKa = 11.84ITYY31 pKa = 10.44NDD33 pKa = 3.57LCRR36 pKa = 11.84GCTHH40 pKa = 6.4SCKK43 pKa = 10.03QSFRR47 pKa = 11.84AVIILCPRR55 pKa = 11.84YY56 pKa = 8.96YY57 pKa = 10.38SKK59 pKa = 10.69RR60 pKa = 11.84RR61 pKa = 11.84KK62 pKa = 10.02KK63 pKa = 10.07EE64 pKa = 3.49DD65 pKa = 3.47RR66 pKa = 11.84DD67 pKa = 3.51NGRR70 pKa = 3.64

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3276

0

3276

943759

23

3350

288.1

32.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.283 ± 0.048

1.722 ± 0.019

5.743 ± 0.036

7.037 ± 0.046

3.874 ± 0.029

7.201 ± 0.04

1.936 ± 0.023

5.914 ± 0.043

5.553 ± 0.042

9.659 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.018

3.589 ± 0.027

3.749 ± 0.022

3.323 ± 0.024

5.544 ± 0.047

5.875 ± 0.033

5.633 ± 0.051

6.818 ± 0.033

0.993 ± 0.015

3.741 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski