Pediococcus claussenii (strain ATCC BAA-344 / DSM 14800 / JCM 18046 / KCTC 3811 / P06)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1877 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G8PCH7|G8PCH7_PEDCP Putative membrane protein insertion efficiency factor OS=Pediococcus claussenii (strain ATCC BAA-344 / DSM 14800 / JCM 18046 / KCTC 3811 / P06) OX=701521 GN=PECL_670 PE=3 SV=1
MM1 pKa = 7.42 SFWRR5 pKa = 11.84 KK6 pKa = 7.2 NHH8 pKa = 6.45 KK9 pKa = 8.84 STNLNTKK16 pKa = 7.89 NHH18 pKa = 5.34 YY19 pKa = 10.85 KK20 pKa = 9.61 MYY22 pKa = 10.47 KK23 pKa = 10.0 AGKK26 pKa = 7.78 QWLFAGITTIAGLGGQSIAGAKK48 pKa = 9.79 ADD50 pKa = 4.08 SLSATSTATKK60 pKa = 10.18 DD61 pKa = 3.6 VQDD64 pKa = 3.8 QDD66 pKa = 3.8 VLATGDD72 pKa = 3.67 SAIIPAEE79 pKa = 3.99 SSVASSSVATASATSATLSSASSAVSSSNSSNTSSASASSADD121 pKa = 3.68 TSSSSSSVASGSNTTSSSVSSTSSSSATSSANASSNNSNSSSATSSTVNSSSNSAATSSTAEE183 pKa = 3.87 SSSPVSSSSSVASITSNALSSATSGTQSLVSSGVTNTTSSSATTSSTATSSSAVASLSMSNSMSLSEE250 pKa = 4.14 SQSLISLASLNIGSSVAASSAASSAKK276 pKa = 9.35 KK277 pKa = 10.09 VAMKK281 pKa = 10.74 AMVMAATVAPTVAVNVGSLSYY302 pKa = 11.86 NMGTQIAYY310 pKa = 9.56 YY311 pKa = 10.64 ALRR314 pKa = 11.84 SWKK317 pKa = 10.37 DD318 pKa = 2.94 SGLNIVSLTPSWLLANFTAISALLTYY344 pKa = 9.26 YY345 pKa = 10.01 QANGTVLRR353 pKa = 11.84 ADD355 pKa = 3.46 GTMDD359 pKa = 4.0 PSLIAALSGIYY370 pKa = 9.58 AAPSWSGQTGIKK382 pKa = 10.38 VSGSTAASSGYY393 pKa = 6.49 TQNGSGWNNTDD404 pKa = 3.13 YY405 pKa = 11.62 TNDD408 pKa = 3.25 YY409 pKa = 10.34 YY410 pKa = 11.59 LIGTVKK416 pKa = 10.64 NPFSATDD423 pKa = 3.62 YY424 pKa = 9.59 YY425 pKa = 11.65 AGIASILNVYY435 pKa = 8.78 AQEE438 pKa = 3.93 VTNYY442 pKa = 9.25 FDD444 pKa = 3.63 QVATKK449 pKa = 10.6 VQDD452 pKa = 3.35 TSGGVGNSLSDD463 pKa = 3.35 QLASYY468 pKa = 7.49 FTYY471 pKa = 10.78 NPNIAQNQSGALGLLANVTALVSPLLSAQGSNDD504 pKa = 3.31 SLTGQIPGIFGFHH517 pKa = 5.56 VTTSDD522 pKa = 3.52 PLIISDD528 pKa = 4.3 LTQNASNAYY537 pKa = 8.15 NTGSLQSLDD546 pKa = 3.64 NLISVVMSSLQPAIEE561 pKa = 4.02 KK562 pKa = 10.55 QLFNDD567 pKa = 3.28 IRR569 pKa = 11.84 TLGINQSGSLSIPSDD584 pKa = 3.92 LYY586 pKa = 9.14 TAVYY590 pKa = 9.44 TLTPGLAQVISYY602 pKa = 10.01 FNFSSLLNLPLLNQIYY618 pKa = 10.22 LSVVNGIRR626 pKa = 11.84 TAIVNDD632 pKa = 4.58 AIVGVNAAFKK642 pKa = 10.86 NILDD646 pKa = 4.52 PKK648 pKa = 10.57 DD649 pKa = 4.28 NPDD652 pKa = 3.58 PDD654 pKa = 3.86 YY655 pKa = 11.21 STVAGYY661 pKa = 10.78 NRR663 pKa = 11.84 TSASGAANTVQTTQNTTLLAQSVGYY688 pKa = 9.95 SWAQQVVPTILRR700 pKa = 11.84 AASADD705 pKa = 3.67 ALNKK709 pKa = 8.25 ATPQNLNEE717 pKa = 4.41 ILNTLLNAGVITSAQVTQLTVAGTQNTSYY746 pKa = 10.07 TNYY749 pKa = 10.06 VADD752 pKa = 3.58 PTGAQNLITSLYY764 pKa = 7.23 TAEE767 pKa = 4.1 YY768 pKa = 10.35 NAVQQAVTDD777 pKa = 4.26 FNNGTPKK784 pKa = 10.48 GANPTWTDD792 pKa = 3.15 PSTVQTDD799 pKa = 3.75 GTNSGIEE806 pKa = 3.97 TLTDD810 pKa = 3.13 YY811 pKa = 11.81 GNVYY815 pKa = 10.64 DD816 pKa = 4.14 YY817 pKa = 11.15 LVKK820 pKa = 10.39 VRR822 pKa = 11.84 HH823 pKa = 6.33 DD824 pKa = 3.72 AMVDD828 pKa = 3.14 ADD830 pKa = 4.11 AKK832 pKa = 10.64 SHH834 pKa = 5.49 SEE836 pKa = 3.78 KK837 pKa = 10.48 DD838 pKa = 3.48 GKK840 pKa = 10.39 VAALTSVSGSTNANFNTTAGLTNYY864 pKa = 7.09 VTNAVSASGILSSTSDD880 pKa = 3.01 ISALAAISQTDD891 pKa = 3.66 YY892 pKa = 10.47 VSEE895 pKa = 4.37 YY896 pKa = 10.1 NAEE899 pKa = 3.97 AALTNTAFAQGAAAAQVQIRR919 pKa = 11.84 LHH921 pKa = 6.3 PNGHH925 pKa = 6.54 LASPASVDD933 pKa = 3.38 YY934 pKa = 10.94 NGYY937 pKa = 10.02 SGSSDD942 pKa = 2.71 KK943 pKa = 10.44 TAQYY947 pKa = 11.62 AVTTDD952 pKa = 3.42 TLQTGQAFTDD962 pKa = 5.04 GYY964 pKa = 9.82 LASVDD969 pKa = 3.86 TVTEE973 pKa = 4.03 IAAPSAPTSSWTTAQLTAFNTAIKK997 pKa = 10.49 NAFPNATQQSNGNWVIGTDD1016 pKa = 3.92 YY1017 pKa = 10.77 VAPGATNVLYY1027 pKa = 8.0 TAPTTVTGFGSTNQTLTTAGAAGNTSDD1054 pKa = 3.44 TVTFTFTAGVNLISNYY1070 pKa = 10.43 GIDD1073 pKa = 4.06 GASSTGLGQDD1083 pKa = 3.23 TSFTPTGTALTIPGLLTGLNGEE1105 pKa = 4.17 LQTWHH1110 pKa = 6.57 NASKK1114 pKa = 10.69 ILFDD1118 pKa = 4.42 FNNTTFSTTSNGVTGSSLSIYY1139 pKa = 10.27 LLAYY1143 pKa = 8.94 YY1144 pKa = 10.02 AQLGYY1149 pKa = 9.33 ATATDD1154 pKa = 4.1 LQTAISNEE1162 pKa = 3.95 FASGSDD1168 pKa = 3.2 KK1169 pKa = 11.17 LALQVLALIQGGLATTSAASSGTVTMNYY1197 pKa = 10.2 AFKK1200 pKa = 9.54 NTNVNSTVATVGSIVDD1216 pKa = 3.33 QRR1218 pKa = 11.84 GNKK1221 pKa = 8.45 LQPDD1225 pKa = 4.07 TTNSLVVGNNLVFQTPTIPTGRR1247 pKa = 11.84 TINFDD1252 pKa = 3.04 NSYY1255 pKa = 10.85 VQMSLDD1261 pKa = 2.97 GSEE1264 pKa = 4.05 PVKK1267 pKa = 10.41 YY1268 pKa = 8.89 TLTQLTQILGITLTGNSQADD1288 pKa = 3.66 LTSIINAFNEE1298 pKa = 3.92 ANGNVTITTGNLLDD1312 pKa = 4.08 NLQALTYY1319 pKa = 10.14 IQLNPQASTASVSGSQSVTYY1339 pKa = 9.36 TGSVPALTTGDD1350 pKa = 3.89 YY1351 pKa = 10.35 TVTLADD1357 pKa = 3.6 GSTYY1361 pKa = 10.16 TLQAGDD1367 pKa = 3.44 IDD1369 pKa = 4.95 IINSSKK1375 pKa = 10.86 NAGTYY1380 pKa = 7.73 QVEE1383 pKa = 4.36 LTAQGLANINAASADD1398 pKa = 3.92 HH1399 pKa = 7.3 IYY1401 pKa = 10.61 TIDD1404 pKa = 3.56 NSTLGTLVINKK1415 pKa = 9.51 ANATINVDD1423 pKa = 3.66 GTQHH1427 pKa = 6.48 VYY1429 pKa = 10.01 TGDD1432 pKa = 3.67 TYY1434 pKa = 11.7 NATATATGAVNGEE1447 pKa = 4.18 TLNYY1451 pKa = 8.21 TVSGSQTEE1459 pKa = 4.37 IGSSPVAVNFDD1470 pKa = 3.62 PADD1473 pKa = 4.72 PINANYY1479 pKa = 10.39 NITVTGAAVITVVRR1493 pKa = 11.84 NATSDD1498 pKa = 3.81 SNSTSASEE1506 pKa = 4.22 SMSDD1510 pKa = 3.55 LGSVSASDD1518 pKa = 3.37 ITSVSQSNSIAGSISISNSNNFSASNSIVEE1548 pKa = 4.45 SASTSASKK1556 pKa = 10.58 QSSIEE1561 pKa = 4.16 TNDD1564 pKa = 3.34 KK1565 pKa = 10.75 VSEE1568 pKa = 4.08 LMSDD1572 pKa = 3.48 SASLSEE1578 pKa = 4.06 EE1579 pKa = 3.68 ASAGGSQSLQNSQSLSNSMSTNGDD1603 pKa = 3.1 NSISNVNSLSEE1614 pKa = 4.44 SNSLSAQNSLSIAQGNSLNNEE1635 pKa = 3.85 LSDD1638 pKa = 4.07 AEE1640 pKa = 4.39 SGVASGDD1647 pKa = 3.54 TSQSNSVNDD1656 pKa = 4.16 AGSQSLVNSVSEE1668 pKa = 4.41 SNSIIDD1674 pKa = 3.78 RR1675 pKa = 11.84 GSDD1678 pKa = 3.28 GSNDD1682 pKa = 3.91 SISNSNSYY1690 pKa = 10.86 SVANSNSVQNSISASNSLSLDD1711 pKa = 3.16 DD1712 pKa = 4.65 SNNVNSDD1719 pKa = 2.94 TSEE1722 pKa = 4.19 SVSLSISNSQNIVNSVSTSTSGSTSFSMSGNEE1754 pKa = 4.11 SVSGSTSISEE1764 pKa = 4.35 SEE1766 pKa = 4.29 SLSSVDD1772 pKa = 3.63 SVSGSVSNSNNVSNTTSASLSLSNSNSEE1800 pKa = 4.19 STVEE1804 pKa = 4.09 SLSVSNSLSGFAKK1817 pKa = 10.55 CC1818 pKa = 5.43
Molecular weight: 185.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.837
IPC2_protein 3.986
IPC_protein 4.024
Toseland 3.795
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.846
Grimsley 3.694
Solomon 4.012
Lehninger 3.973
Nozaki 4.126
DTASelect 4.406
Thurlkill 3.846
EMBOSS 3.961
Sillero 4.139
Patrickios 0.693
IPC_peptide 4.012
IPC2_peptide 4.126
IPC2.peptide.svr19 4.013
Protein with the highest isoelectric point:
>tr|G8PE98|G8PE98_PEDCP NADH oxidase OS=Pediococcus claussenii (strain ATCC BAA-344 / DSM 14800 / JCM 18046 / KCTC 3811 / P06) OX=701521 GN=nox PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.69 QPKK8 pKa = 8.69 KK9 pKa = 7.77 RR10 pKa = 11.84 HH11 pKa = 4.99 RR12 pKa = 11.84 QRR14 pKa = 11.84 VHH16 pKa = 6.07 GFRR19 pKa = 11.84 KK20 pKa = 10.03 RR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 RR29 pKa = 11.84 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.8 VLSAA44 pKa = 4.11
Molecular weight: 5.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.464
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.398
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.135
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.099
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1877
0
1877
566800
21
3609
302.0
33.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.024 ± 0.128
0.379 ± 0.011
5.874 ± 0.069
5.944 ± 0.078
4.496 ± 0.047
6.606 ± 0.055
1.878 ± 0.025
7.832 ± 0.073
6.809 ± 0.07
9.401 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.708 ± 0.032
5.541 ± 0.052
3.314 ± 0.031
4.232 ± 0.046
4.052 ± 0.056
6.698 ± 0.134
5.745 ± 0.063
7.146 ± 0.049
0.978 ± 0.021
3.344 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here