Octadecabacter temperatus
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3276 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K0Y2K1|A0A0K0Y2K1_9RHOB Uncharacterized protein OS=Octadecabacter temperatus OX=1458307 GN=OSB_05890 PE=4 SV=1
MM1 pKa = 7.0 KK2 pKa = 9.72 TLFIGTAIAMTAVTSAAAQEE22 pKa = 4.13 LSYY25 pKa = 11.36 GRR27 pKa = 11.84 LIADD31 pKa = 4.29 FEE33 pKa = 4.49 MLSTPGGDD41 pKa = 3.21 VNASILGAEE50 pKa = 4.23 AGVRR54 pKa = 11.84 VQDD57 pKa = 3.21 FDD59 pKa = 3.41 FWINGTQVTISPDD72 pKa = 3.37 GVPVNLTADD81 pKa = 3.3 VSTIGAGYY89 pKa = 7.67 TFSGNYY95 pKa = 9.11 RR96 pKa = 11.84 VDD98 pKa = 3.34 VSSSNLGFGIGGTGLSIGLDD118 pKa = 4.22 EE119 pKa = 6.14 IGVAYY124 pKa = 10.45 DD125 pKa = 2.84 NGTFFGRR132 pKa = 11.84 LSFADD137 pKa = 3.18 INQPVPGVDD146 pKa = 3.29 GLLGLHH152 pKa = 6.54 VGYY155 pKa = 10.54 QFNDD159 pKa = 3.45 YY160 pKa = 10.67 ADD162 pKa = 3.5 VSMSVHH168 pKa = 6.54 ALDD171 pKa = 4.06 EE172 pKa = 4.43 TSGTVDD178 pKa = 3.46 PLIILGGEE186 pKa = 4.18 YY187 pKa = 9.18 DD188 pKa = 3.38 TGQWGVKK195 pKa = 10.0 AEE197 pKa = 4.08 YY198 pKa = 10.66 ASISLGGTDD207 pKa = 5.07 LSLISLAGNYY217 pKa = 9.17 QINDD221 pKa = 3.01 QWGVRR226 pKa = 11.84 GAYY229 pKa = 8.03 STADD233 pKa = 3.21 IAGTDD238 pKa = 3.24 ADD240 pKa = 5.16 LIRR243 pKa = 11.84 LGGTYY248 pKa = 10.3 NVNDD252 pKa = 4.56 KK253 pKa = 9.12 YY254 pKa = 9.67 TLYY257 pKa = 10.88 AGLSRR262 pKa = 11.84 VDD264 pKa = 3.53 LGGFEE269 pKa = 4.21 VDD271 pKa = 3.95 GFNIGMTMDD280 pKa = 4.84 FGDD283 pKa = 4.1 KK284 pKa = 9.39 PASYY288 pKa = 7.7 EE289 pKa = 4.04 TTADD293 pKa = 3.55 RR294 pKa = 11.84 LLGVIEE300 pKa = 3.97 TAGAFDD306 pKa = 3.89 YY307 pKa = 11.38
Molecular weight: 32.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.732
IPC_protein 3.757
Toseland 3.528
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.164
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.884
Patrickios 0.871
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A0K0Y1Z3|A0A0K0Y1Z3_9RHOB Uncharacterized protein OS=Octadecabacter temperatus OX=1458307 GN=OSB_03600 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.25 GRR39 pKa = 11.84 AEE41 pKa = 3.9 LSAA44 pKa = 4.93
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3276
0
3276
992371
30
3928
302.9
32.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.519 ± 0.057
0.93 ± 0.015
6.459 ± 0.059
5.887 ± 0.045
3.92 ± 0.032
8.549 ± 0.049
1.972 ± 0.021
5.603 ± 0.035
3.493 ± 0.041
9.515 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.948 ± 0.026
3.089 ± 0.024
4.701 ± 0.029
3.207 ± 0.021
5.688 ± 0.042
5.441 ± 0.027
5.977 ± 0.052
7.481 ± 0.038
1.351 ± 0.019
2.271 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here