Conidiobolus coronatus (strain ATCC 28846 / CBS 209.66 / NRRL 28638) (Delacroixia coronata)
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10567 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A137P700|A0A137P700_CONC2 Vacuolar transporter chaperone 4 OS=Conidiobolus coronatus (strain ATCC 28846 / CBS 209.66 / NRRL 28638) OX=796925 GN=CONCODRAFT_58054 PE=4 SV=1
NN1 pKa = 7.46 EE2 pKa = 3.97 GDD4 pKa = 3.27 KK5 pKa = 11.53 AKK7 pKa = 10.36 DD8 pKa = 3.64 YY9 pKa = 10.74 KK10 pKa = 11.0 SKK12 pKa = 10.47 PVYY15 pKa = 9.59 STEE18 pKa = 4.86 DD19 pKa = 3.24 VDD21 pKa = 5.88 DD22 pKa = 4.58 YY23 pKa = 11.45 SAKK26 pKa = 10.21 PSYY29 pKa = 10.49 SDD31 pKa = 3.53 KK32 pKa = 11.08 DD33 pKa = 3.82 YY34 pKa = 11.51 SDD36 pKa = 4.77 EE37 pKa = 4.14 VDD39 pKa = 4.22 DD40 pKa = 4.57 YY41 pKa = 11.27 KK42 pKa = 11.42 SKK44 pKa = 10.69 PIYY47 pKa = 9.5 EE48 pKa = 4.81 DD49 pKa = 4.02 KK50 pKa = 11.1 DD51 pKa = 4.19 DD52 pKa = 4.11 NDD54 pKa = 3.69 EE55 pKa = 4.14 VDD57 pKa = 4.91 DD58 pKa = 4.13 YY59 pKa = 12.14 SSVKK63 pKa = 9.88 PYY65 pKa = 10.35 KK66 pKa = 10.71 SKK68 pKa = 10.78 DD69 pKa = 3.7 YY70 pKa = 10.46 PSEE73 pKa = 5.08 PILNTEE79 pKa = 3.77 VDD81 pKa = 4.72 DD82 pKa = 4.33 FDD84 pKa = 6.47 SMPLYY89 pKa = 9.71 PGKK92 pKa = 10.63 VDD94 pKa = 4.51 DD95 pKa = 5.62 YY96 pKa = 11.12 EE97 pKa = 5.17 DD98 pKa = 4.81 FYY100 pKa = 10.98 PTYY103 pKa = 10.39 EE104 pKa = 4.61 KK105 pKa = 11.04 VDD107 pKa = 4.01 DD108 pKa = 4.3 YY109 pKa = 12.0 SNEE112 pKa = 4.11 DD113 pKa = 3.54 DD114 pKa = 4.94 CDD116 pKa = 3.85 TAVEE120 pKa = 5.11 DD121 pKa = 4.01 YY122 pKa = 11.27 SSDD125 pKa = 3.03 KK126 pKa = 11.01 SYY128 pKa = 11.88 SNDD131 pKa = 3.06 YY132 pKa = 10.63 VSNSKK137 pKa = 10.48 YY138 pKa = 10.22 GKK140 pKa = 10.22 QYY142 pKa = 11.0 DD143 pKa = 4.08 EE144 pKa = 4.69 EE145 pKa = 5.18 SDD147 pKa = 3.41 IVVVFIEE154 pKa = 4.83 DD155 pKa = 3.33 SDD157 pKa = 4.92 GEE159 pKa = 4.38 LEE161 pKa = 4.17 MLISSEE167 pKa = 4.27 DD168 pKa = 3.26
Molecular weight: 19.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.465
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.872
Wikipedia 3.643
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.075
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.821
Patrickios 0.769
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A137PHE8|A0A137PHE8_CONC2 Ras-related protein rab-21-like protein OS=Conidiobolus coronatus (strain ATCC 28846 / CBS 209.66 / NRRL 28638) OX=796925 GN=CONCODRAFT_54725 PE=4 SV=1
CC1 pKa = 7.39 ANCFSTTTPLWRR13 pKa = 11.84 RR14 pKa = 11.84 DD15 pKa = 3.49 PQGRR19 pKa = 11.84 TICNACGLYY28 pKa = 10.23 YY29 pKa = 10.84 KK30 pKa = 10.18 LNKK33 pKa = 8.08 VHH35 pKa = 7.31 RR36 pKa = 11.84 PACLKK41 pKa = 10.09 QDD43 pKa = 3.79 VIRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 3.12
Molecular weight: 5.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.648
IPC_protein 10.555
Toseland 10.628
ProMoST 10.891
Dawson 10.745
Bjellqvist 10.526
Wikipedia 10.979
Rodwell 10.877
Grimsley 10.804
Solomon 10.891
Lehninger 10.847
Nozaki 10.701
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.701
Patrickios 10.672
IPC_peptide 10.891
IPC2_peptide 10.043
IPC2.peptide.svr19 8.697
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10563
4
10567
3652955
49
4552
345.7
39.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.188 ± 0.024
1.44 ± 0.011
5.251 ± 0.016
5.981 ± 0.024
4.708 ± 0.02
5.143 ± 0.025
2.132 ± 0.012
7.267 ± 0.023
7.049 ± 0.023
10.178 ± 0.03
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.936 ± 0.01
6.554 ± 0.029
4.698 ± 0.023
4.058 ± 0.02
3.717 ± 0.015
8.601 ± 0.029
5.699 ± 0.025
5.351 ± 0.017
1.091 ± 0.008
3.958 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here