Amino acid dipepetide frequency for Virgibacillus salinus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.09AlaAla: 5.09 ± 0.093
0.549AlaCys: 0.549 ± 0.025
3.539AlaAsp: 3.539 ± 0.056
4.433AlaGlu: 4.433 ± 0.081
3.201AlaPhe: 3.201 ± 0.058
5.018AlaGly: 5.018 ± 0.083
1.214AlaHis: 1.214 ± 0.036
5.848AlaIle: 5.848 ± 0.073
4.403AlaLys: 4.403 ± 0.068
6.482AlaLeu: 6.482 ± 0.082
1.993AlaMet: 1.993 ± 0.051
2.947AlaAsn: 2.947 ± 0.055
1.936AlaPro: 1.936 ± 0.05
2.03AlaGln: 2.03 ± 0.036
2.411AlaArg: 2.411 ± 0.057
4.009AlaSer: 4.009 ± 0.062
3.521AlaThr: 3.521 ± 0.057
5.123AlaVal: 5.123 ± 0.084
0.604AlaTrp: 0.604 ± 0.023
2.228AlaTyr: 2.228 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.377CysAla: 0.377 ± 0.021
0.077CysCys: 0.077 ± 0.01
0.393CysAsp: 0.393 ± 0.018
0.406CysGlu: 0.406 ± 0.02
0.279CysPhe: 0.279 ± 0.015
0.584CysGly: 0.584 ± 0.025
0.158CysHis: 0.158 ± 0.013
0.468CysIle: 0.468 ± 0.021
0.342CysLys: 0.342 ± 0.016
0.536CysLeu: 0.536 ± 0.021
0.174CysMet: 0.174 ± 0.012
0.276CysAsn: 0.276 ± 0.016
0.287CysPro: 0.287 ± 0.018
0.188CysGln: 0.188 ± 0.016
0.205CysArg: 0.205 ± 0.013
0.436CysSer: 0.436 ± 0.021
0.339CysThr: 0.339 ± 0.019
0.369CysVal: 0.369 ± 0.018
0.056CysTrp: 0.056 ± 0.007
0.225CysTyr: 0.225 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
3.337AspAla: 3.337 ± 0.058
0.315AspCys: 0.315 ± 0.017
3.005AspAsp: 3.005 ± 0.064
4.89AspGlu: 4.89 ± 0.074
2.592AspPhe: 2.592 ± 0.048
3.626AspGly: 3.626 ± 0.07
1.112AspHis: 1.112 ± 0.034
4.627AspIle: 4.627 ± 0.071
3.86AspLys: 3.86 ± 0.065
4.95AspLeu: 4.95 ± 0.067
1.556AspMet: 1.556 ± 0.042
2.545AspAsn: 2.545 ± 0.059
2.046AspPro: 2.046 ± 0.05
2.131AspGln: 2.131 ± 0.049
2.199AspArg: 2.199 ± 0.049
2.994AspSer: 2.994 ± 0.058
2.76AspThr: 2.76 ± 0.049
4.004AspVal: 4.004 ± 0.07
0.701AspTrp: 0.701 ± 0.024
2.331AspTyr: 2.331 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
5.015GluAla: 5.015 ± 0.08
0.322GluCys: 0.322 ± 0.021
4.1GluAsp: 4.1 ± 0.075
6.757GluGlu: 6.757 ± 0.116
2.622GluPhe: 2.622 ± 0.057
4.191GluGly: 4.191 ± 0.067
1.454GluHis: 1.454 ± 0.029
5.687GluIle: 5.687 ± 0.077
6.476GluLys: 6.476 ± 0.091
6.841GluLeu: 6.841 ± 0.09
2.276GluMet: 2.276 ± 0.037
4.268GluAsn: 4.268 ± 0.074
1.975GluPro: 1.975 ± 0.043
3.441GluGln: 3.441 ± 0.072
3.251GluArg: 3.251 ± 0.073
4.091GluSer: 4.091 ± 0.065
3.933GluThr: 3.933 ± 0.072
5.128GluVal: 5.128 ± 0.07
0.797GluTrp: 0.797 ± 0.027
2.298GluTyr: 2.298 ± 0.053
0.0GluXaa: 0.0 ± 0.0
Phe
2.947PheAla: 2.947 ± 0.05
0.295PheCys: 0.295 ± 0.017
2.498PheAsp: 2.498 ± 0.053
2.74PheGlu: 2.74 ± 0.057
2.329PhePhe: 2.329 ± 0.057
3.233PheGly: 3.233 ± 0.063
0.962PheHis: 0.962 ± 0.031
4.174PheIle: 4.174 ± 0.07
2.452PheLys: 2.452 ± 0.053
4.378PheLeu: 4.378 ± 0.084
1.209PheMet: 1.209 ± 0.033
2.172PheAsn: 2.172 ± 0.048
1.664PhePro: 1.664 ± 0.04
1.611PheGln: 1.611 ± 0.035
1.456PheArg: 1.456 ± 0.036
3.301PheSer: 3.301 ± 0.063
2.62PheThr: 2.62 ± 0.059
2.991PheVal: 2.991 ± 0.06
0.458PheTrp: 0.458 ± 0.022
1.779PheTyr: 1.779 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.732GlyAla: 4.732 ± 0.073
0.514GlyCys: 0.514 ± 0.023
3.491GlyAsp: 3.491 ± 0.058
4.681GlyGlu: 4.681 ± 0.08
3.381GlyPhe: 3.381 ± 0.056
4.807GlyGly: 4.807 ± 0.084
1.373GlyHis: 1.373 ± 0.041
6.001GlyIle: 6.001 ± 0.1
4.828GlyLys: 4.828 ± 0.073
6.419GlyLeu: 6.419 ± 0.095
2.175GlyMet: 2.175 ± 0.045
2.94GlyAsn: 2.94 ± 0.063
1.736GlyPro: 1.736 ± 0.041
1.993GlyGln: 1.993 ± 0.046
2.388GlyArg: 2.388 ± 0.046
4.193GlySer: 4.193 ± 0.066
3.914GlyThr: 3.914 ± 0.063
5.005GlyVal: 5.005 ± 0.074
0.841GlyTrp: 0.841 ± 0.032
2.797GlyTyr: 2.797 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.304HisAla: 1.304 ± 0.037
0.169HisCys: 0.169 ± 0.013
1.093HisAsp: 1.093 ± 0.037
1.308HisGlu: 1.308 ± 0.037
0.967HisPhe: 0.967 ± 0.031
1.345HisGly: 1.345 ± 0.038
0.572HisHis: 0.572 ± 0.024
1.545HisIle: 1.545 ± 0.037
1.101HisLys: 1.101 ± 0.034
1.926HisLeu: 1.926 ± 0.04
0.566HisMet: 0.566 ± 0.022
0.906HisAsn: 0.906 ± 0.028
1.009HisPro: 1.009 ± 0.03
0.79HisGln: 0.79 ± 0.026
0.817HisArg: 0.817 ± 0.026
1.108HisSer: 1.108 ± 0.033
1.042HisThr: 1.042 ± 0.032
1.434HisVal: 1.434 ± 0.035
0.198HisTrp: 0.198 ± 0.014
0.812HisTyr: 0.812 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.224IleAla: 6.224 ± 0.08
0.601IleCys: 0.601 ± 0.026
4.813IleAsp: 4.813 ± 0.072
5.625IleGlu: 5.625 ± 0.074
3.461IlePhe: 3.461 ± 0.077
6.188IleGly: 6.188 ± 0.1
1.675IleHis: 1.675 ± 0.039
6.725IleIle: 6.725 ± 0.122
4.781IleLys: 4.781 ± 0.072
7.179IleLeu: 7.179 ± 0.106
1.926IleMet: 1.926 ± 0.049
3.962IleAsn: 3.962 ± 0.067
3.43IlePro: 3.43 ± 0.056
2.882IleGln: 2.882 ± 0.052
3.015IleArg: 3.015 ± 0.06
5.316IleSer: 5.316 ± 0.078
4.722IleThr: 4.722 ± 0.074
5.626IleVal: 5.626 ± 0.086
0.682IleTrp: 0.682 ± 0.029
2.666IleTyr: 2.666 ± 0.051
0.0IleXaa: 0.0 ± 0.0
Lys
4.269LysAla: 4.269 ± 0.074
0.283LysCys: 0.283 ± 0.019
4.082LysAsp: 4.082 ± 0.068
6.661LysGlu: 6.661 ± 0.086
1.963LysPhe: 1.963 ± 0.051
4.145LysGly: 4.145 ± 0.065
1.403LysHis: 1.403 ± 0.035
4.668LysIle: 4.668 ± 0.077
5.688LysLys: 5.688 ± 0.09
5.995LysLeu: 5.995 ± 0.081
2.181LysMet: 2.181 ± 0.054
3.635LysAsn: 3.635 ± 0.063
2.096LysPro: 2.096 ± 0.049
3.334LysGln: 3.334 ± 0.069
3.044LysArg: 3.044 ± 0.07
3.965LysSer: 3.965 ± 0.059
3.577LysThr: 3.577 ± 0.056
4.446LysVal: 4.446 ± 0.062
0.789LysTrp: 0.789 ± 0.028
2.189LysTyr: 2.189 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
6.661LeuAla: 6.661 ± 0.091
0.532LeuCys: 0.532 ± 0.02
4.952LeuAsp: 4.952 ± 0.07
6.353LeuGlu: 6.353 ± 0.085
4.754LeuPhe: 4.754 ± 0.085
6.333LeuGly: 6.333 ± 0.089
1.749LeuHis: 1.749 ± 0.045
7.448LeuIle: 7.448 ± 0.109
6.132LeuLys: 6.132 ± 0.081
9.54LeuLeu: 9.54 ± 0.116
2.509LeuMet: 2.509 ± 0.051
4.456LeuAsn: 4.456 ± 0.067
3.747LeuPro: 3.747 ± 0.064
3.396LeuGln: 3.396 ± 0.058
3.337LeuArg: 3.337 ± 0.061
6.405LeuSer: 6.405 ± 0.09
5.532LeuThr: 5.532 ± 0.072
6.053LeuVal: 6.053 ± 0.076
0.76LeuTrp: 0.76 ± 0.027
3.105LeuTyr: 3.105 ± 0.06
0.0LeuXaa: 0.0 ± 0.0
Met
1.986MetAla: 1.986 ± 0.043
0.133MetCys: 0.133 ± 0.011
1.683MetAsp: 1.683 ± 0.041
2.123MetGlu: 2.123 ± 0.047
1.125MetPhe: 1.125 ± 0.037
1.813MetGly: 1.813 ± 0.042
0.484MetHis: 0.484 ± 0.025
2.148MetIle: 2.148 ± 0.048
2.396MetLys: 2.396 ± 0.048
2.588MetLeu: 2.588 ± 0.056
0.807MetMet: 0.807 ± 0.029
1.672MetAsn: 1.672 ± 0.034
0.978MetPro: 0.978 ± 0.031
1.032MetGln: 1.032 ± 0.034
1.039MetArg: 1.039 ± 0.029
1.723MetSer: 1.723 ± 0.037
1.642MetThr: 1.642 ± 0.036
1.858MetVal: 1.858 ± 0.044
0.191MetTrp: 0.191 ± 0.014
0.803MetTyr: 0.803 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
2.917AsnAla: 2.917 ± 0.054
0.311AsnCys: 0.311 ± 0.018
2.86AsnAsp: 2.86 ± 0.051
3.997AsnGlu: 3.997 ± 0.071
1.893AsnPhe: 1.893 ± 0.043
3.566AsnGly: 3.566 ± 0.065
1.055AsnHis: 1.055 ± 0.035
3.814AsnIle: 3.814 ± 0.073
3.468AsnLys: 3.468 ± 0.063
4.109AsnLeu: 4.109 ± 0.058
1.368AsnMet: 1.368 ± 0.033
2.748AsnAsn: 2.748 ± 0.066
2.184AsnPro: 2.184 ± 0.048
2.25AsnGln: 2.25 ± 0.052
2.11AsnArg: 2.11 ± 0.048
2.656AsnSer: 2.656 ± 0.058
2.531AsnThr: 2.531 ± 0.051
3.29AsnVal: 3.29 ± 0.057
0.581AsnTrp: 0.581 ± 0.023
1.889AsnTyr: 1.889 ± 0.045
0.0AsnXaa: 0.0 ± 0.0
Pro
2.129ProAla: 2.129 ± 0.046
0.177ProCys: 0.177 ± 0.014
2.159ProAsp: 2.159 ± 0.05
3.023ProGlu: 3.023 ± 0.059
1.881ProPhe: 1.881 ± 0.043
2.316ProGly: 2.316 ± 0.056
0.708ProHis: 0.708 ± 0.025
2.873ProIle: 2.873 ± 0.054
2.216ProLys: 2.216 ± 0.048
3.221ProLeu: 3.221 ± 0.064
0.856ProMet: 0.856 ± 0.029
1.737ProAsn: 1.737 ± 0.043
0.985ProPro: 0.985 ± 0.036
1.044ProGln: 1.044 ± 0.033
1.044ProArg: 1.044 ± 0.034
2.228ProSer: 2.228 ± 0.048
1.864ProThr: 1.864 ± 0.038
2.76ProVal: 2.76 ± 0.057
0.395ProTrp: 0.395 ± 0.021
1.39ProTyr: 1.39 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
2.532GlnAla: 2.532 ± 0.052
0.16GlnCys: 0.16 ± 0.013
1.948GlnAsp: 1.948 ± 0.043
2.948GlnGlu: 2.948 ± 0.062
1.637GlnPhe: 1.637 ± 0.041
2.172GlnGly: 2.172 ± 0.049
0.776GlnHis: 0.776 ± 0.028
2.6GlnIle: 2.6 ± 0.054
2.691GlnLys: 2.691 ± 0.054
3.95GlnLeu: 3.95 ± 0.071
1.134GlnMet: 1.134 ± 0.029
1.835GlnAsn: 1.835 ± 0.04
1.176GlnPro: 1.176 ± 0.034
1.904GlnGln: 1.904 ± 0.057
1.37GlnArg: 1.37 ± 0.04
2.282GlnSer: 2.282 ± 0.052
2.057GlnThr: 2.057 ± 0.043
2.434GlnVal: 2.434 ± 0.051
0.417GlnTrp: 0.417 ± 0.02
1.217GlnTyr: 1.217 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
2.17ArgAla: 2.17 ± 0.056
0.222ArgCys: 0.222 ± 0.017
2.033ArgAsp: 2.033 ± 0.045
2.908ArgGlu: 2.908 ± 0.059
1.789ArgPhe: 1.789 ± 0.045
2.161ArgGly: 2.161 ± 0.054
0.728ArgHis: 0.728 ± 0.027
2.984ArgIle: 2.984 ± 0.057
3.005ArgLys: 3.005 ± 0.065
3.6ArgLeu: 3.6 ± 0.072
1.219ArgMet: 1.219 ± 0.036
2.019ArgAsn: 2.019 ± 0.045
1.187ArgPro: 1.187 ± 0.034
1.43ArgGln: 1.43 ± 0.042
1.615ArgArg: 1.615 ± 0.045
2.124ArgSer: 2.124 ± 0.05
1.967ArgThr: 1.967 ± 0.047
2.538ArgVal: 2.538 ± 0.055
0.404ArgTrp: 0.404 ± 0.02
1.466ArgTyr: 1.466 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
3.628SerAla: 3.628 ± 0.057
0.309SerCys: 0.309 ± 0.019
3.317SerAsp: 3.317 ± 0.058
4.29SerGlu: 4.29 ± 0.066
3.201SerPhe: 3.201 ± 0.064
4.609SerGly: 4.609 ± 0.076
1.186SerHis: 1.186 ± 0.032
5.471SerIle: 5.471 ± 0.077
3.816SerLys: 3.816 ± 0.062
5.93SerLeu: 5.93 ± 0.083
1.72SerMet: 1.72 ± 0.039
2.983SerAsn: 2.983 ± 0.056
2.112SerPro: 2.112 ± 0.049
1.994SerGln: 1.994 ± 0.047
2.261SerArg: 2.261 ± 0.049
4.134SerSer: 4.134 ± 0.081
3.353SerThr: 3.353 ± 0.059
4.29SerVal: 4.29 ± 0.064
0.645SerTrp: 0.645 ± 0.025
2.376SerTyr: 2.376 ± 0.047
0.0SerXaa: 0.0 ± 0.0
Thr
3.862ThrAla: 3.862 ± 0.072
0.346ThrCys: 0.346 ± 0.019
3.065ThrAsp: 3.065 ± 0.061
3.743ThrGlu: 3.743 ± 0.078
2.67ThrPhe: 2.67 ± 0.052
4.267ThrGly: 4.267 ± 0.063
1.087ThrHis: 1.087 ± 0.032
4.902ThrIle: 4.902 ± 0.068
3.396ThrLys: 3.396 ± 0.063
5.074ThrLeu: 5.074 ± 0.074
1.373ThrMet: 1.373 ± 0.038
2.773ThrAsn: 2.773 ± 0.047
2.122ThrPro: 2.122 ± 0.047
1.512ThrGln: 1.512 ± 0.041
1.72ThrArg: 1.72 ± 0.041
3.419ThrSer: 3.419 ± 0.067
3.005ThrThr: 3.005 ± 0.062
4.093ThrVal: 4.093 ± 0.066
0.531ThrTrp: 0.531 ± 0.023
2.027ThrTyr: 2.027 ± 0.045
0.0ThrXaa: 0.0 ± 0.0
Val
4.725ValAla: 4.725 ± 0.079
0.545ValCys: 0.545 ± 0.024
3.864ValAsp: 3.864 ± 0.069
4.812ValGlu: 4.812 ± 0.067
3.158ValPhe: 3.158 ± 0.062
4.637ValGly: 4.637 ± 0.071
1.274ValHis: 1.274 ± 0.037
5.992ValIle: 5.992 ± 0.083
4.481ValLys: 4.481 ± 0.071
6.502ValLeu: 6.502 ± 0.08
1.943ValMet: 1.943 ± 0.039
3.412ValAsn: 3.412 ± 0.061
2.649ValPro: 2.649 ± 0.051
2.291ValGln: 2.291 ± 0.051
2.435ValArg: 2.435 ± 0.056
4.503ValSer: 4.503 ± 0.069
4.211ValThr: 4.211 ± 0.07
4.982ValVal: 4.982 ± 0.066
0.646ValTrp: 0.646 ± 0.028
2.272ValTyr: 2.272 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.605TrpAla: 0.605 ± 0.023
0.06TrpCys: 0.06 ± 0.008
0.576TrpAsp: 0.576 ± 0.026
0.724TrpGlu: 0.724 ± 0.025
0.519TrpPhe: 0.519 ± 0.025
0.693TrpGly: 0.693 ± 0.028
0.184TrpHis: 0.184 ± 0.015
0.863TrpIle: 0.863 ± 0.032
0.721TrpLys: 0.721 ± 0.03
1.128TrpLeu: 1.128 ± 0.035
0.316TrpMet: 0.316 ± 0.014
0.558TrpAsn: 0.558 ± 0.024
0.259TrpPro: 0.259 ± 0.016
0.379TrpGln: 0.379 ± 0.023
0.353TrpArg: 0.353 ± 0.02
0.571TrpSer: 0.571 ± 0.023
0.498TrpThr: 0.498 ± 0.023
0.682TrpVal: 0.682 ± 0.029
0.139TrpTrp: 0.139 ± 0.012
0.393TrpTyr: 0.393 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.069TyrAla: 2.069 ± 0.047
0.255TyrCys: 0.255 ± 0.014
2.111TyrAsp: 2.111 ± 0.05
2.502TyrGlu: 2.502 ± 0.062
1.848TyrPhe: 1.848 ± 0.05
2.508TyrGly: 2.508 ± 0.053
0.859TyrHis: 0.859 ± 0.027
2.664TyrIle: 2.664 ± 0.06
2.156TyrLys: 2.156 ± 0.045
3.405TyrLeu: 3.405 ± 0.059
0.919TyrMet: 0.919 ± 0.033
1.755TyrAsn: 1.755 ± 0.049
1.427TyrPro: 1.427 ± 0.039
1.61TyrGln: 1.61 ± 0.043
1.501TyrArg: 1.501 ± 0.039
2.166TyrSer: 2.166 ± 0.043
1.899TyrThr: 1.899 ± 0.045
2.226TyrVal: 2.226 ± 0.036
0.385TyrTrp: 0.385 ± 0.021
1.52TyrTyr: 1.52 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3649 proteins (1048091 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski